BBa_M31101 1 BBa_M31101 CrtI gene 2008-04-30T11:00:00Z 2015-05-08T01:13:59Z This gene was taken from the genome of the bacterium Solfolobus solfataricus and has been codon optimized for the lactobacillus acidophilus strain NCFM. The CrtI gene produces the enzyme phytoene desaturase, which converts phytoene to lycopene. It is found in the carotenoid synthesis pathway. false false _102_ 0 1392 102 Not in stock false Contact biomagik@mit.edu for specific questions. false biomagik@mit BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M333334 1 BBa_M333334 Produces Beta-carotene from the biochemical input GGPP 2008-05-05T11:00:00Z 2015-05-08T01:14:01Z Each gene is taken from a bacterial strain in which the carotenoid synthesis pathway is present. CrtB-R capsulatus CrtI-S solfataricus CrtY-C pelagibacter This part contains 3 genes, each of which codes for an enzyme in the carotenoid synthesis pathway: CrtB (Phytoene synthase), CrtI (Phytoene desaturase) and CrtY (Lycopene cyclase). The conversion process is GGPP->Phytoene->Lycopene->B-carotene Composite order: 1) Constitutive promoter BBa_J23100 2) CrtY 3) RBS BBa_B0034 4) CrtI 5) RBS BBa_J23100 6) CrtB 7) Terminator B0015 false false _102_48_ 0 52 167 Not in stock false Contact biomagik@mit.edu for specific questions. false biomagik@mit component1962410 1 BBa_J23100 component1962411 1 BBa_M31100 component1962420 1 BBa_B0012 component1962414 1 BBa_M31101 component1962417 1 BBa_M31102 component1962416 1 BBa_B0034 component1962413 1 BBa_B0034 component1962418 1 BBa_B0010 annotation1962414 1 BBa_M31101 range1962414 1 1088 2770 annotation1962411 1 BBa_M31100 range1962411 1 42 1061 annotation1962413 1 BBa_B0034 range1962413 1 1070 1081 annotation1962418 1 BBa_B0010 range1962418 1 3837 3916 annotation1962417 1 BBa_M31102 range1962417 1 2797 3828 annotation1962420 1 BBa_B0012 range1962420 1 3925 3965 annotation1962410 1 BBa_J23100 range1962410 1 1 35 annotation1962416 1 BBa_B0034 range1962416 1 2779 2790 BBa_M31100 1 BBa_M31100 CrtB gene 2008-04-30T11:00:00Z 2015-05-08T01:13:59Z This gene is taken from the genome of the bacterium R capsulatus. This part encodes for the CrtB gene, which produces the phytoene synthase enzyme in the carotenoid synthesis pathway. This enzyme converts Geranylgeranyl diphosphate (GGPP) to phytoene. false false _102_ 0 1392 102 Not in stock false Contact biomagik@mit.edu for specific questions. false biomagik@mit BBa_M31102 1 BBa_M31102 CrtY gene 2008-04-30T11:00:00Z 2015-05-08T01:13:59Z This gene was taken from the genome of the bacterium C pelagibacter. The CrtY gene produces the enzyme lycopene cyclase, which converts lycopene to beta carotene in the carotenoid synthesis pathway. false false _102_ 0 1392 102 Not in stock false Contact biomagik@mit.edu for further details. false biomagik@mit BBa_J23100 1 BBa_J23100 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z Isolated from library of promoters Released HQ 2013 Replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_J23100_sequence 1 ttgacggctagctcagtcctaggtacagtgctagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M333334_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagatgattgctgaagctgatatggaagtttgtcgtgaattaattcgtactggttcatattcatttcatgctgcttcacgtgttttaccagctcgtgttcgtgatccagctttagctttatatgctttttgtcgtgttgctgatgatgaagttgatgaagttggtgctccacgtgataaagctgctgctgttttaaaattaggtgatcgtttagaagatatttatgctggtcgtccacgtaatgctccatcagatcgtgcttttgctgctgttgttgaagaatttgaaatgccacgtgaattaccagaagctttattagaaggttttgcttgggatgctgaaggtcgttggtatcatactttatcagatgttcaagcttattcagctcgtgttgctgctgctgttggtgctatgatgtgtgttttaatgcgtgttcgtaatccagatgctttagctcgtgcttgtgatttaggtttagctatgcaaatgtcaaatattgctcgtgatgttggtgaagatgctcgtgctggtcgtttatttttaccaactgattggatggttgaagaaggtattgatccacaagcttttttagctgatccacaaccaactaaaggtattcgtcgtgttactgaacgtttattaaatcgtgctgatcgtttatattggcgtgctgctactggtgttcgtttattaccatttgattgtcgtccaggtattatggctgctggtaaaatttatgctgctattggtgctgaagttgctaaagctaaatatgataatattactcgtcgtgctcatactactaaaggtcgtaaattatggttagttgctaattcagctatgtcagctactgctacttcaatgttaccattatcaccacgtgttcatgctaaaccagaaccagaagttgctcatttagttgatgctgctgctcatcgtaatttacatccagaacgttcagaagttttaatttcagctttaatggctttaaaagctcgtgatcgtggtttagctatggattagtactagagaaagaggagaaatactagatgatgaaagctgttgttgttggtgctggtttaggtggtatttcaactgctattttattaaaaaaattttttgatgaagttgttgttttagaaaaaaatactactccaggtggtcgtgctcgttatttttcagttggtgaatttcgttttgatatgggtccatcatggtatttaatgccagaaatttttgaagaatttttttcagaagctggtgaaaatccacatccaattattcgtgttaatccaccagttaaagttatggaatcaaaatttttaaaaaaagaatatcgtgaatttactggtgataatgttgatgatcgttatttaaataaaactcgtttattttatgatttttcaaaaaaatttattaaaaaagaaatggttttttcagattttttagataaagaagttttagttaatatttcagctttatttgatactttagataattttaatgctcgttattttcgtgattcattaatgcgtaaagctatgggtttttcatcagtttttattggtggttcaccatttcgtgttccagctgtttatgctatgattaattatgctatttatggtcaaggtgttttttatccatataaaggttttgaaggttatgttaaacgtttatatgaaattgctcgtaaaaaaggtgttgaatttaaatttgattttccagttaataaagttaaaattgatgaaggtaaagttgttgaagtttcatcaaatattaaacgtgaaatgggtgatgtttttgtttttaatatggattatttttatgctgattcattattaccagaagaatataaagttatttcaggtcgtcgttataaattatcaccatcagctattttagcttatattggtttagaagatactttagatttaccacatcatactgttattattaatggtgattggcgtgaacattttacttcaattgatcgtaattcattaccatcagttgaaaatacttcatattatgtttcatatcgtggtgctactaataaagaaactcgtgatttagttattttaattccagttggtactggtactgaaatttcaaaagaattaattgatggtgttattcgtgatttagaagttaaaattggttgtaaaattaaagcttcatatattcgtatttatggtccagatgattttaaacgtgattataatgcttatgaaggtactgcttttggtttagctcatactttagatcaaactggtccatttcgtttaccaatgaaaaatactcgtttaaaaaatttatattatgttggtcaatatactcaaccaggtattggtgttccaatggttactttatcatcaatgattgtttcaaaaaaaatttcaaaagaatttttaatgatgaaagctgttgttgttggtgctggtttaggtggtatttcaactgctattttattaaaaaaattttttgatgaagttgttgttttagaaaaaaatactactccaggtggtcgtgctcgttatttttcagttggtgaatttcgttttgatatgggtccatcatggtatttaatgccagaaatttttgaagaatttttttcagaagctggtgaaaatccacatccaattattcgtgttaatccaccagttaaagttatggaatcaaaatttttaaaaaaagaatatcgtgaatttactggtgataatgttgatgattagtactagagaaagaggagaaatactagatgtcattagcttatgaattagatttacataataaattaaaagatgctactttagctattattgaaccacgtttagaatataaacgtgataaaacttggtcatattggaaaactattgatcataattttcaagattgtgttcaaaaatcatggaataatttttcaattaatattccaggtaaaactaaatttttagaatgtaatggtttaccatatgaagctattgattcaggtttattttatgaaaaaattaatactcgtttaaaaaaaaataaaaatatttcattttataaaaatactaatgaaattgatttaaattcagcttttatttttaattcagttccattattagattcaaaagaagataaattatggcaacattttaaaggtattgaaattgaaactaaaaaaaatgtttttgatgatgaaatttttaatttaatggattttgattgtgatcaacgtgattcagttcattttttttatactttaccatatactaaaaataaagctttaattgaaactacttggttatcaaaaattaaaaatgattcattaaaagattatgatgaacaattaaaaaattatattcgtaatcatttaaatattcgtgattataaagttacttattcagaagttggtgctattccattatttcatccaactatttcaaatgaacgtaataaaatgaatattggtactgctggtggtatgactcgtttatcaactggttatacttttttaaatattcaagaacattcaaaatatattcgtaaaaatattcatactattaaagaatcaaaacgttatgaaattaataaaaaatataaatttttagataaaatttttttaaaagttttaaattatcatccagaaaaaatggctaatattttttttgatatgtttaataattcaccagaaactattattaaatttttatcaaataaatcaactttattacaagatttatttattattttaaaaatgccaaaatggttatttattaaaattttttttaaaaatgataaaatgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_M31100_sequence 1 atgattgctgaagctgatatggaagtttgtcgtgaattaattcgtactggttcatattcatttcatgctgcttcacgtgttttaccagctcgtgttcgtgatccagctttagctttatatgctttttgtcgtgttgctgatgatgaagttgatgaagttggtgctccacgtgataaagctgctgctgttttaaaattaggtgatcgtttagaagatatttatgctggtcgtccacgtaatgctccatcagatcgtgcttttgctgctgttgttgaagaatttgaaatgccacgtgaattaccagaagctttattagaaggttttgcttgggatgctgaaggtcgttggtatcatactttatcagatgttcaagcttattcagctcgtgttgctgctgctgttggtgctatgatgtgtgttttaatgcgtgttcgtaatccagatgctttagctcgtgcttgtgatttaggtttagctatgcaaatgtcaaatattgctcgtgatgttggtgaagatgctcgtgctggtcgtttatttttaccaactgattggatggttgaagaaggtattgatccacaagcttttttagctgatccacaaccaactaaaggtattcgtcgtgttactgaacgtttattaaatcgtgctgatcgtttatattggcgtgctgctactggtgttcgtttattaccatttgattgtcgtccaggtattatggctgctggtaaaatttatgctgctattggtgctgaagttgctaaagctaaatatgataatattactcgtcgtgctcatactactaaaggtcgtaaattatggttagttgctaattcagctatgtcagctactgctacttcaatgttaccattatcaccacgtgttcatgctaaaccagaaccagaagttgctcatttagttgatgctgctgctcatcgtaatttacatccagaacgttcagaagttttaatttcagctttaatggctttaaaagctcgtgatcgtggtttagctatggattag BBa_M31102_sequence 1 atgtcattagcttatgaattagatttacataataaattaaaagatgctactttagctattattgaaccacgtttagaatataaacgtgataaaacttggtcatattggaaaactattgatcataattttcaagattgtgttcaaaaatcatggaataatttttcaattaatattccaggtaaaactaaatttttagaatgtaatggtttaccatatgaagctattgattcaggtttattttatgaaaaaattaatactcgtttaaaaaaaaataaaaatatttcattttataaaaatactaatgaaattgatttaaattcagcttttatttttaattcagttccattattagattcaaaagaagataaattatggcaacattttaaaggtattgaaattgaaactaaaaaaaatgtttttgatgatgaaatttttaatttaatggattttgattgtgatcaacgtgattcagttcattttttttatactttaccatatactaaaaataaagctttaattgaaactacttggttatcaaaaattaaaaatgattcattaaaagattatgatgaacaattaaaaaattatattcgtaatcatttaaatattcgtgattataaagttacttattcagaagttggtgctattccattatttcatccaactatttcaaatgaacgtaataaaatgaatattggtactgctggtggtatgactcgtttatcaactggttatacttttttaaatattcaagaacattcaaaatatattcgtaaaaatattcatactattaaagaatcaaaacgttatgaaattaataaaaaatataaatttttagataaaatttttttaaaagttttaaattatcatccagaaaaaatggctaatattttttttgatatgtttaataattcaccagaaactattattaaatttttatcaaataaatcaactttattacaagatttatttattattttaaaaatgccaaaatggttatttattaaaattttttttaaaaatgataaaatg BBa_B0034_sequence 1 aaagaggagaaa BBa_M31101_sequence 1 atgatgaaagctgttgttgttggtgctggtttaggtggtatttcaactgctattttattaaaaaaattttttgatgaagttgttgttttagaaaaaaatactactccaggtggtcgtgctcgttatttttcagttggtgaatttcgttttgatatgggtccatcatggtatttaatgccagaaatttttgaagaatttttttcagaagctggtgaaaatccacatccaattattcgtgttaatccaccagttaaagttatggaatcaaaatttttaaaaaaagaatatcgtgaatttactggtgataatgttgatgatcgttatttaaataaaactcgtttattttatgatttttcaaaaaaatttattaaaaaagaaatggttttttcagattttttagataaagaagttttagttaatatttcagctttatttgatactttagataattttaatgctcgttattttcgtgattcattaatgcgtaaagctatgggtttttcatcagtttttattggtggttcaccatttcgtgttccagctgtttatgctatgattaattatgctatttatggtcaaggtgttttttatccatataaaggttttgaaggttatgttaaacgtttatatgaaattgctcgtaaaaaaggtgttgaatttaaatttgattttccagttaataaagttaaaattgatgaaggtaaagttgttgaagtttcatcaaatattaaacgtgaaatgggtgatgtttttgtttttaatatggattatttttatgctgattcattattaccagaagaatataaagttatttcaggtcgtcgttataaattatcaccatcagctattttagcttatattggtttagaagatactttagatttaccacatcatactgttattattaatggtgattggcgtgaacattttacttcaattgatcgtaattcattaccatcagttgaaaatacttcatattatgtttcatatcgtggtgctactaataaagaaactcgtgatttagttattttaattccagttggtactggtactgaaatttcaaaagaattaattgatggtgttattcgtgatttagaagttaaaattggttgtaaaattaaagcttcatatattcgtatttatggtccagatgattttaaacgtgattataatgcttatgaaggtactgcttttggtttagctcatactttagatcaaactggtccatttcgtttaccaatgaaaaatactcgtttaaaaaatttatattatgttggtcaatatactcaaccaggtattggtgttccaatggttactttatcatcaatgattgtttcaaaaaaaatttcaaaagaatttttaatgatgaaagctgttgttgttggtgctggtttaggtggtatttcaactgctattttattaaaaaaattttttgatgaagttgttgttttagaaaaaaatactactccaggtggtcgtgctcgttatttttcagttggtgaatttcgttttgatatgggtccatcatggtatttaatgccagaaatttttgaagaatttttttcagaagctggtgaaaatccacatccaattattcgtgttaatccaccagttaaagttatggaatcaaaatttttaaaaaaagaatatcgtgaatttactggtgataatgttgatgattag BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z