BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M33352 1 BBa_M33352 CDS for TodS protein 2010-06-05T11:00:00Z 2015-05-08T01:14:02Z The sequence was found at http://www.ncbi.nlm.nih.gov/nuccore/Y18245.1 (TodS coordinates: 11292..14228). Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed Ptod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated with an increase in the rate of transphosphorylation of TodT. Ptod is activated once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates. This part should be used in conjunction with BBa_M33351, the Ptod promoter. false false _577_ 0 6929 9 Not in stock false We added restriction digestion sites and made 3 silent mutations, 1 for EcoRI and 2 for PstI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA. false Sunny Kim annotation2070203 1 todS range2070203 1 1 2937 BBa_M33350 1 BBa_M33350 RBS+CDS for TodS protein 2010-06-05T11:00:00Z 2015-05-08T01:14:01Z The sequence of the Ptod promoter was given in the following article. Lacal, J., Guazzaroni, M. E., Busch, A., Krell, T. & Ramos, J. L. (2008). Hierarchical binding of the TodT response regulator to its multiple recognition sites at the tod pathway operon promoter. J. Mol. Biol. 376, 325???337. This identicle sequence was found upstream of the todX gene sequence natural existent in Pseudomonas putida given in http://www.ncbi.nlm.nih.gov/nuccore/AB042508.1 (todX coordinates: 14325..15686). Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. The catabolic genes of the toluene dioxygenase pathway are transcribed from a single promoter termed Ptod, located upstream from the todX gene. The TodS/TodT TCS mediate Ptod in response to toluene and other aromatic hydrocarbons. Ptod is activated once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates. This part should be used in conjunction with false false _577_ 0 6929 9 Not in stock false The sequence was checked for restriction digest sites to ensure compliance with Assembly Standard 10. false Sunny Kim component2070216 1 BBa_M33352 component2070214 1 BBa_B0034 annotation2070214 1 BBa_B0034 range2070214 1 1 12 annotation2070216 1 BBa_M33352 range2070216 1 19 2955 BBa_B0034_sequence 1 aaagaggagaaa BBa_M33352_sequence 1 atgagctccttggatagaaaaaagcctcaaaatagatcgaaaaataattattataatatctgcctcaaggagaaaggatctgaagagctgacgtgtgaagaacatgcacgcatcatatttgatgggctctacgagtttgtgggccttcttgatgctcatggaaatgtgcttgaagtgaaccaggtcgcattggaggggggcgggattactctggaagaaatacgagggaagccattctggaaggcgcgttggtggcaaatttcaaaaaaaaccgaggcgacccaaaagcgacttgttgaaactgcatcatccggtgaatttgttcgctgtgatgttgagattcttggaaaatcaggtggaagagaggtaatatcggtcgatttttcattgctgccaatttgcaatgaagaagggagcattgtttaccttcttgcggaagggcgcaatattaccgataagaagaaagccgaggccatgctggcgttgaagaaccaggaattggagcagtcggttgagtgtatccgaaaactcgataatgcgaagagtgatttctttgccaaggtgagccatgagttgcgcactccgctgtctttgattctaggccactggaagccgttatggcgggcagaggctgggcgtgaatcgccgtattggaagcagtttgaggtcattcagcgtaatgcaatgaccctgttgaaacaggttaacacgctgcttgacttggcgaaaatggacgcccggcagatggggctttcctatcggcgagccaatcttagtcagctcacccgtactattagctcgaattttgaaggaatagcccagcaaaaatcaataacgttcgatacaaaactgcctgtacagatggtcgctgaggtggattgtgagaaatacgaacgcattatccttaacttgctttccaatgcgtttaaattcacccctgacggggggcttatccgttgctgtcttagtttgagtcgaccaaattatgccttggttactgtatctgatagcgggccgggtattcctcctgcactgcgtaaagaaatatttgaacgtttccaccagctaagccaggaaggtcaacaagctacgcggggtacaggcttggggctttccattgtgaaagagttcgttgaattgcaccgtggaacaatttctgtaagtgatgccccgggcgggggggcgctttttcaggtaaagctgccgctgaatgctcctgaaggtgcttatgttgcgagtaacaccgcgccgcgaagagataatcctcaggtcgtggatacggatgagtaccttttgctggcgcccaatgcggaaaatgaagccgaggtgcttccatttcaatccgaccagcctcgggtgctaatcgttgaagataaccctgatatgcgtggttttataaaggactgtctcagtagcgactatcaagtttatgttgcacccgacggtgcaaaggcattggagttgatgtcaaacatgccgccagacctgttgattacagacctgatgatgcctgttatgagcggcgatatgctggttcaccaagtgcgtaagaaaaatgaactttcacatatcccgatcatggtgctgtcggccaagtcagacgcagaactgcgtgtgaaattgctctccgagtcggtgcaggactttcttcttaagccattttctgctcatgagctacgagcgcgtgtaagcaatctggtatccatgaaggtggcaggcgatgcgttgcgtaaggagctttccgatcagggggatgatattgcgatacttactcaccgtctgatcaaaagtcgccatcgtcttcagcagagtaacatcgcattatccgcctcggaagcgcgttggaaagcagtgtatgaaaactctgcggccggtattgtactgaccgacccggaaaaccgaatactcaacgccaatcctgcatttcaacgcattaccggatatggggaaaaggatttggagggactttccatggagcaattgactccatctgacgaaagcccacagataaagcagcgtctggccaatttgcttcagggtgggggagcggaatacagtgtggagcgctcctatctatgcaaaaatggttctacgatttgggccaatgcgagtgtctcgctgatgcctcaacgtgtcggtgaatctccagttatactccagatcatcgatgacatcactgagaagaaacaagcacaggaaaatcttaaccaattgcagcaacaacttgtgtacgtttcccgatcagctacgatgggtgaatttgcagcctatattgcacacgagataaaccaaccgctctcggcgatcatgaccaatgccaatgctggcacacgttggttaggtaatgagccatctaacatcccagaggctaaagaggcactggctcgcattatccgagattccgaccgcgccgcagaaattatccgtatggtacgctccttcctgaagcgtcaagaaacggtgctgaaaccgattgatctaaaagcactggtaactgatacaagcctgatacttaaggcccctagtcagaataacagtgtcaatttggatgttgttgcggatgatgaactccctgagatatggggggatggtgtacagatccagcagttgataataaatctggctatgaacgctattgaagcgatcagccaagccgactgtgaaaccaggcagctaaccctgtcattctcaggcaatgatacaggtgatgcgcttgttatctcagtgaaagatacaggtccaggtatttcagagaggcagatggcgcagttgttcaacgcattctacaccacaaaaaaagaagggcttggtatgggattggcaatctgtcttacaatcacggaagtgcataacggtaaaatatgggttgagtgcccgcccgctgggggtgcttgtttcctggtaagtatccctgccagacagggctccggcacataa BBa_M33350_sequence 1 aaagaggagaaatactagatgagctccttggatagaaaaaagcctcaaaatagatcgaaaaataattattataatatctgcctcaaggagaaaggatctgaagagctgacgtgtgaagaacatgcacgcatcatatttgatgggctctacgagtttgtgggccttcttgatgctcatggaaatgtgcttgaagtgaaccaggtcgcattggaggggggcgggattactctggaagaaatacgagggaagccattctggaaggcgcgttggtggcaaatttcaaaaaaaaccgaggcgacccaaaagcgacttgttgaaactgcatcatccggtgaatttgttcgctgtgatgttgagattcttggaaaatcaggtggaagagaggtaatatcggtcgatttttcattgctgccaatttgcaatgaagaagggagcattgtttaccttcttgcggaagggcgcaatattaccgataagaagaaagccgaggccatgctggcgttgaagaaccaggaattggagcagtcggttgagtgtatccgaaaactcgataatgcgaagagtgatttctttgccaaggtgagccatgagttgcgcactccgctgtctttgattctaggccactggaagccgttatggcgggcagaggctgggcgtgaatcgccgtattggaagcagtttgaggtcattcagcgtaatgcaatgaccctgttgaaacaggttaacacgctgcttgacttggcgaaaatggacgcccggcagatggggctttcctatcggcgagccaatcttagtcagctcacccgtactattagctcgaattttgaaggaatagcccagcaaaaatcaataacgttcgatacaaaactgcctgtacagatggtcgctgaggtggattgtgagaaatacgaacgcattatccttaacttgctttccaatgcgtttaaattcacccctgacggggggcttatccgttgctgtcttagtttgagtcgaccaaattatgccttggttactgtatctgatagcgggccgggtattcctcctgcactgcgtaaagaaatatttgaacgtttccaccagctaagccaggaaggtcaacaagctacgcggggtacaggcttggggctttccattgtgaaagagttcgttgaattgcaccgtggaacaatttctgtaagtgatgccccgggcgggggggcgctttttcaggtaaagctgccgctgaatgctcctgaaggtgcttatgttgcgagtaacaccgcgccgcgaagagataatcctcaggtcgtggatacggatgagtaccttttgctggcgcccaatgcggaaaatgaagccgaggtgcttccatttcaatccgaccagcctcgggtgctaatcgttgaagataaccctgatatgcgtggttttataaaggactgtctcagtagcgactatcaagtttatgttgcacccgacggtgcaaaggcattggagttgatgtcaaacatgccgccagacctgttgattacagacctgatgatgcctgttatgagcggcgatatgctggttcaccaagtgcgtaagaaaaatgaactttcacatatcccgatcatggtgctgtcggccaagtcagacgcagaactgcgtgtgaaattgctctccgagtcggtgcaggactttcttcttaagccattttctgctcatgagctacgagcgcgtgtaagcaatctggtatccatgaaggtggcaggcgatgcgttgcgtaaggagctttccgatcagggggatgatattgcgatacttactcaccgtctgatcaaaagtcgccatcgtcttcagcagagtaacatcgcattatccgcctcggaagcgcgttggaaagcagtgtatgaaaactctgcggccggtattgtactgaccgacccggaaaaccgaatactcaacgccaatcctgcatttcaacgcattaccggatatggggaaaaggatttggagggactttccatggagcaattgactccatctgacgaaagcccacagataaagcagcgtctggccaatttgcttcagggtgggggagcggaatacagtgtggagcgctcctatctatgcaaaaatggttctacgatttgggccaatgcgagtgtctcgctgatgcctcaacgtgtcggtgaatctccagttatactccagatcatcgatgacatcactgagaagaaacaagcacaggaaaatcttaaccaattgcagcaacaacttgtgtacgtttcccgatcagctacgatgggtgaatttgcagcctatattgcacacgagataaaccaaccgctctcggcgatcatgaccaatgccaatgctggcacacgttggttaggtaatgagccatctaacatcccagaggctaaagaggcactggctcgcattatccgagattccgaccgcgccgcagaaattatccgtatggtacgctccttcctgaagcgtcaagaaacggtgctgaaaccgattgatctaaaagcactggtaactgatacaagcctgatacttaaggcccctagtcagaataacagtgtcaatttggatgttgttgcggatgatgaactccctgagatatggggggatggtgtacagatccagcagttgataataaatctggctatgaacgctattgaagcgatcagccaagccgactgtgaaaccaggcagctaaccctgtcattctcaggcaatgatacaggtgatgcgcttgttatctcagtgaaagatacaggtccaggtatttcagagaggcagatggcgcagttgttcaacgcattctacaccacaaaaaaagaagggcttggtatgggattggcaatctgtcttacaatcacggaagtgcataacggtaaaatatgggttgagtgcccgcccgctgggggtgcttgtttcctggtaagtatccctgccagacagggctccggcacataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z