BBa_M33352 1 BBa_M33352 CDS for TodS protein 2010-06-05T11:00:00Z 2015-05-08T01:14:02Z The sequence was found at http://www.ncbi.nlm.nih.gov/nuccore/Y18245.1 (TodS coordinates: 11292..14228). Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. In response to toluene and other aromatic hydrocarbons, the TodS/TodT TCS mediate a single promoter termed Ptod, which transcribes the catabolic genes involved in the toluene dioxygenase pathway. TodS exhibits basal autophosphorylation activity that increases in the presence of toluene and is translated with an increase in the rate of transphosphorylation of TodT. Ptod is activated once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates. This part should be used in conjunction with BBa_M33351, the Ptod promoter. false false _577_ 0 6929 9 Not in stock false We added restriction digestion sites and made 3 silent mutations, 1 for EcoRI and 2 for PstI, to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA. false Sunny Kim annotation2070203 1 todS range2070203 1 1 2937 BBa_M33352_sequence 1 atgagctccttggatagaaaaaagcctcaaaatagatcgaaaaataattattataatatctgcctcaaggagaaaggatctgaagagctgacgtgtgaagaacatgcacgcatcatatttgatgggctctacgagtttgtgggccttcttgatgctcatggaaatgtgcttgaagtgaaccaggtcgcattggaggggggcgggattactctggaagaaatacgagggaagccattctggaaggcgcgttggtggcaaatttcaaaaaaaaccgaggcgacccaaaagcgacttgttgaaactgcatcatccggtgaatttgttcgctgtgatgttgagattcttggaaaatcaggtggaagagaggtaatatcggtcgatttttcattgctgccaatttgcaatgaagaagggagcattgtttaccttcttgcggaagggcgcaatattaccgataagaagaaagccgaggccatgctggcgttgaagaaccaggaattggagcagtcggttgagtgtatccgaaaactcgataatgcgaagagtgatttctttgccaaggtgagccatgagttgcgcactccgctgtctttgattctaggccactggaagccgttatggcgggcagaggctgggcgtgaatcgccgtattggaagcagtttgaggtcattcagcgtaatgcaatgaccctgttgaaacaggttaacacgctgcttgacttggcgaaaatggacgcccggcagatggggctttcctatcggcgagccaatcttagtcagctcacccgtactattagctcgaattttgaaggaatagcccagcaaaaatcaataacgttcgatacaaaactgcctgtacagatggtcgctgaggtggattgtgagaaatacgaacgcattatccttaacttgctttccaatgcgtttaaattcacccctgacggggggcttatccgttgctgtcttagtttgagtcgaccaaattatgccttggttactgtatctgatagcgggccgggtattcctcctgcactgcgtaaagaaatatttgaacgtttccaccagctaagccaggaaggtcaacaagctacgcggggtacaggcttggggctttccattgtgaaagagttcgttgaattgcaccgtggaacaatttctgtaagtgatgccccgggcgggggggcgctttttcaggtaaagctgccgctgaatgctcctgaaggtgcttatgttgcgagtaacaccgcgccgcgaagagataatcctcaggtcgtggatacggatgagtaccttttgctggcgcccaatgcggaaaatgaagccgaggtgcttccatttcaatccgaccagcctcgggtgctaatcgttgaagataaccctgatatgcgtggttttataaaggactgtctcagtagcgactatcaagtttatgttgcacccgacggtgcaaaggcattggagttgatgtcaaacatgccgccagacctgttgattacagacctgatgatgcctgttatgagcggcgatatgctggttcaccaagtgcgtaagaaaaatgaactttcacatatcccgatcatggtgctgtcggccaagtcagacgcagaactgcgtgtgaaattgctctccgagtcggtgcaggactttcttcttaagccattttctgctcatgagctacgagcgcgtgtaagcaatctggtatccatgaaggtggcaggcgatgcgttgcgtaaggagctttccgatcagggggatgatattgcgatacttactcaccgtctgatcaaaagtcgccatcgtcttcagcagagtaacatcgcattatccgcctcggaagcgcgttggaaagcagtgtatgaaaactctgcggccggtattgtactgaccgacccggaaaaccgaatactcaacgccaatcctgcatttcaacgcattaccggatatggggaaaaggatttggagggactttccatggagcaattgactccatctgacgaaagcccacagataaagcagcgtctggccaatttgcttcagggtgggggagcggaatacagtgtggagcgctcctatctatgcaaaaatggttctacgatttgggccaatgcgagtgtctcgctgatgcctcaacgtgtcggtgaatctccagttatactccagatcatcgatgacatcactgagaagaaacaagcacaggaaaatcttaaccaattgcagcaacaacttgtgtacgtttcccgatcagctacgatgggtgaatttgcagcctatattgcacacgagataaaccaaccgctctcggcgatcatgaccaatgccaatgctggcacacgttggttaggtaatgagccatctaacatcccagaggctaaagaggcactggctcgcattatccgagattccgaccgcgccgcagaaattatccgtatggtacgctccttcctgaagcgtcaagaaacggtgctgaaaccgattgatctaaaagcactggtaactgatacaagcctgatacttaaggcccctagtcagaataacagtgtcaatttggatgttgttgcggatgatgaactccctgagatatggggggatggtgtacagatccagcagttgataataaatctggctatgaacgctattgaagcgatcagccaagccgactgtgaaaccaggcagctaaccctgtcattctcaggcaatgatacaggtgatgcgcttgttatctcagtgaaagatacaggtccaggtatttcagagaggcagatggcgcagttgttcaacgcattctacaccacaaaaaaagaagggcttggtatgggattggcaatctgtcttacaatcacggaagtgcataacggtaaaatatgggttgagtgcccgcccgctgggggtgcttgtttcctggtaagtatccctgccagacagggctccggcacataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z