LOCUS BBa_M33355 7469 bp DNA linear 23-Dec-2024 DEFINITION pTod + Purple Color Generator ACCESSION BBa_M33355 VERSION BBa_M33355.1 FEATURES Location/Qualifiers promoter 1..116 /label=pTod promoter promoter 1..116 /label=BBa_M33351 RBS 125..141 /label=rbs CDS 142..1398 /label=vioA RBS 1399..1415 /label=rbs CDS 1416..4418 /label=vioB RBS 4419..4435 /label=rbs CDS 4436..5731 /label=vioC RBS 5732..5748 /label=rbs CDS 5749..6876 /label=vioD RBS 6877..6893 /label=rbs CDS 6894..7469 /label=vioE misc_feature 125..7469 /label=BBa_K274002 ORIGIN 1 ggcataaacc atcgtttatc acagttaaac tttggttttc taagttgcga tagccatata 61 aacccataag ccaaaaaaca atatttccca gggcgtgatt gtaatactgt gcgtgctact 121 agagttaagg aggtaaaaaa aatgaaacat tcttccgata tctgcattgt tggtgctggt 181 atttctggtt tgacgtgcgc aagccatctg ctggacagcc cggcatgccg tggtctgagc 241 ctgcgtatct ttgacatgca gcaagaagcc ggtggccgta tccgcagcaa aatgctggat 301 ggtaaggcaa gcattgaact gggcgcaggt cgctactccc ctcagttgca cccgcatttc 361 caaagcgcaa tgcagcacta tagccaaaag agcgaagtct atccgttcac ccagttgaag 421 ttcaaatctc acgtgcagca aaagctgaag cgcgccatga atgaactgtc cccgcgtctg 481 aaagagcatg gtaaagagag ctttttgcag tttgtcagcc gttatcaagg tcacgatagc 541 gcggttggta tgatccgctc tatgggttac gacgcactgt tcctgccgga tatcagcgca 601 gaaatggcct acgacattgt gggtaagcac ccggagatcc agagcgtgac ggacaacgac 661 gcgaaccaat ggtttgcagc ggaaacgggc tttgctggtc tgattcaggg catcaaggct 721 aaggttaagg cggcaggtgc gcgttttagc ctgggttatc gtctgctgag cgtccgtacc 781 gacggtgacg gctacctgct gcaactggca ggtgacgacg gctggaaact ggagcaccgt 841 acccgccatc tgattctggc gattccgccg agcgcgatgg cgggtttgaa tgttgatttt 901 ccagaagcct ggtccggtgc gcgctatggc agcctgccgc tgtttaaggg ctttctgacg 961 tacggtgagc cgtggtggtt ggactacaaa ctggacgatc aggtgctgat tgttgacaac 1021 ccgctgcgca aaatctattt caaaggcgat aagtacctgt tcttctatac cgatagcgag 1081 atggcgaatt actggcgcgg ttgtgtcgcg gagggcgagg acggttacct ggagcaaatt 1141 cgcacccatt tggctagcgc actgggtatc gtccgtgaac gtatcccgca accgctggca 1201 cacgttcaca agtattgggc gcacggcgtt gagttttgcc gtgattctga tattgaccac 1261 ccgagcgcac tgtctcatcg cgacagcggt atcatcgcgt gctccgatgc gtacacggag 1321 cattgtggtt ggatggaggg cggtctgctg agcgcccgtg aggcaagccg tctgctgttg 1381 cagcgtatcg ccgcgtgatt aaggaggtaa aaaaaatgag cattctggat ttcccgcgta 1441 tccacttccg tggctgggcc cgtgtcaatg cgccgaccgc gaaccgcgat ccgcacggcc 1501 acatcgatat ggccagcaat accgtggcga tggcgggtga gccgttcgac ctggcacgcc 1561 atcctacgga gttccaccgt cacctgcgct ccctgggtcc gcgcttcggc ttggatggtc 1621 gtgctgaccc ggaaggcccg ttcagcctgg ccgagggcta caacgctgcc ggtaacaacc 1681 acttttcgtg ggagagcgca accgttagcc acgtgcaatg ggatggcggt gaggcggatc 1741 gtggtgacgg tctggtcggt gctcgtttgg cactgtgggg tcactacaat gattatctgc 1801 gtaccacctt caatcgtgct cgttgggtcg acagcgaccc gacgcgccgt gacgctgcac 1861 aaatctatgc gggccaattc accattagcc cggctggtgc cggtccgggt acgccgtggc 1921 tgtttacggc agacattgat gatagccatg gtgcacgttg gacgcgtggc ggccacattg 1981 cagagcgtgg cggccacttc ttggatgaag agtttggtct ggcacgcctg tttcagttct 2041 ctgtgccgaa agatcaccca cattttctgt ttcacccggg tccgtttgat tccgaggcct 2101 ggcgtcgtct gcaattggct ctggaggatg acgacgttct gggtctgacc gtgcaatatg 2161 cgttgttcaa tatgagcacc ccgcctcagc cgaacagccc ggtttttcac gatatggtcg 2221 gtgttgtcgg tctgtggcgt cgtggtgaac tggcgagcta cccggctggt cgtctgctgc 2281 gtccgcgtca accgggtctg ggtgacctga ccctgcgcgt caacggtggt cgcgttgcgc 2341 tgaatttggc gtgtgccatt ccgttcagca ctcgtgccgc gcagccaagc gcaccggacc 2401 gcctgacccc ggacctgggt gccaaactgc cgctgggcga tctgctgctg cgtgatgagg 2461 acggcgcact gttggcacgt gtgccgcagg ctctgtacca agactattgg acgaatcacg 2521 gtattgtgga cctgccgctg ctgcgcgaac cgcgtggtag cttgaccctg agcagcgaac 2581 tggcggagtg gcgtgagcaa gactgggtca cccaaagcga cgcgtctaac ctgtacctgg 2641 aggcaccgga tcgccgtcac ggtcgctttt tccctgagag catcgcgctg cgcagctact 2701 ttcgcggtga agcgcgtgcg cgtccggata tcccgcatcg tatcgagggc atgggcctgg 2761 tcggcgtcga atctcgtcag gatggcgacg ctgcggaatg gcgtctgacg ggtctgcgtc 2821 cgggtccggc acgcattgtt ctggacgatg gtgccgaggc gatccctctg cgtgttctgc 2881 ctgacgattg ggcgctggat gacgcgaccg tcgaagaagt ggattacgcc tttttgtacc 2941 gccacgttat ggcgtattac gagctggtgt atccattcat gagcgacaag gtgttttccc 3001 tggctgatcg ttgcaaatgt gaaacgtacg cacgtctgat gtggcagatg tgtgatccgc 3061 agaaccgcaa caagtcctat tacatgccga gcacccgcga actgtcggca ccgaaagctc 3121 gtttgttctt gaagtatctg gcccacgtgg aaggccaggc acgcctgcaa gcacctccgc 3181 cagcgggtcc ggcacgcatt gaatctaaag cccagttggc ggcagagctg cgtaaagccg 3241 tcgacctgga gctgtctgtg atgctgcaat acctgtacgc ggcgtatagc attccgaact 3301 atgcacaggg ccaacaacgt gttcgtgacg gtgcgtggac cgccgagcag ctgcaactgg 3361 cgtgcggtag cggtgaccgt cgccgtgatg gcggtattcg tgcagcactg ctggaaattg 3421 ctcatgaaga aatgattcat tacctggtcg ttaacaacct gctgatggcc ctgggcgagc 3481 cgttctacgc gggtgtcccg ctgatgggcg aagcggcacg tcaggcgttt ggcctggaca 3541 ccgagttcgc tctggaaccg tttagcgaaa gcacgctggc acgttttgtt cgtctggaat 3601 ggccgcactt tatcccagca ccgggcaaat ccatcgcgga ctgctatgcc gccattcgtc 3661 aggcgttttt ggatctgccg gacttgtttg gtggcgaggc aggtaagcgt ggcggtgaac 3721 accacctgtt cctgaatgag ctgaccaacc gtgcgcatcc gggttatcaa ctggaagttt 3781 tcgatcgcga ctcggcgctg tttggtattg catttgtgac cgatcagggc gaaggtggcg 3841 ctctggacag cccgcactac gaacatagcc attttcaacg tctgcgtgaa atgagcgcgc 3901 gtatcatggc tcaaagcgca ccgttcgaac cggcgctgcc ggcgttgcgt aatccggttc 3961 tggatgagag cccgggttgc caacgtgtcg cagacggtcg tgcgcgtgcg ctgatggcat 4021 tgtaccaagg cgtttatgag ctgatgtttg cgatgatggc gcagcacttc gccgtgaaac 4081 cgctgggtag cttgcgtcgc agccgcctga tgaacgcagc aatcgatctg atgaccggtc 4141 tgttgcgtcc gctgagctgc gcgctgatga acctgccaag cggcatcgcc ggtcgcacgg 4201 ccggtccgcc gctgccgggt ccggttgaca cccgtagcta tgacgactac gcgctgggct 4261 gtcgcatgct ggcacgccgt tgcgagcgtc tgctggagca ggcgagcatg ctggaaccgg 4321 gttggctgcc ggatgcgcag atggagctgc tggatttcta tcgtcgccaa atgctggact 4381 tggcgtgcgg caaactgagc cgcgaggcct aaggatcctt aaggaggtaa aaaaaatgaa 4441 acgtgcgatt atcgttggtg gcggcctggc gggtggcctg accgcgatct acctggcgaa 4501 gcgtggctac gaagtgcacg tcgtggagaa gcgtggtgat cctctgcgcg atctgagctc 4561 ttacgtggac gttgttagca gccgtgcgat cggcgtgagc atgaccgttc gtggtatcaa 4621 gagcgttttg gctgcgggca ttccgcgtgc agagctggat gcgtgtggcg aaccgatcgt 4681 ggcaatggct ttctccgtgg gtggtcagta tcgcatgcgc gaactgaagc cgttggagga 4741 tttccgtccg ctgagcttga accgtgcggc gtttcaaaag ctgctgaaca aatacgcgaa 4801 cctggcaggc gttcgttact actttgagca taagtgcctg gatgttgacc tggatggtaa 4861 gagcgtgttg attcagggca aagatggtca gccgcagcgt ctgcaaggtg acatgattat 4921 cggtgcggat ggcgcccaca gcgccgtccg tcaggcgatg cagagcggcc tgcgtcgttt 4981 cgagttccag caaacgttct tccgccatgg ctacaaaacc ctggttttgc cggacgcgca 5041 agcactgggt taccgtaaag acacgctgta ctttttcggc atggattccg gtggcctgtt 5101 cgcgggtcgt gcggctacga tcccagatgg tagcgtcagc atcgccgttt gcctgccgta 5161 ctcgggtagc ccttccctga cgaccaccga cgaaccgacg atgcgtgcgt tcttcgatcg 5221 ttacttcggt ggcctgccgc gtgacgcgcg tgacgaaatg ctgcgtcagt ttctggcgaa 5281 gccgagcaac gacctgatta acgtgcgctc tagcaccttt cactataagg gtaatgtgct 5341 gttgctgggt gatgctgcgc atgcgactgc gccgttcctg ggtcagggta tgaacatggc 5401 gctggaggac gcccgcacgt ttgtcgagct gctggaccgc caccagggcg accaagacaa 5461 agcctttccg gagttcacgg agctgcgcaa agtccaggca gacgcaatgc aagacatggc 5521 tcgcgccaac tatgacgttt tgagctgctc gaacccgatc tttttcatgc gtgcgcgtta 5581 cacgcgttac atgcattcca agtttccggg cctgtatccg ccggatatgg ccgagaaact 5641 gtactttacg agcgagccgt acgatcgtct gcaacaaatc cagcgtaaac agaatgtttg 5701 gtacaagatt ggtcgcgtga attgaagatc tttaaggagg taaaaaaaat gaagattctg 5761 gtcattggtg ctggtccagc tggtctggtt ttcgcatccc aactgaagca ggcacgccct 5821 ttgtgggcca ttgacatcgt ggagaagaat gacgagcaag aagtgctggg ctggggtgtc 5881 gtgctgcctg gccgtccggg tcagcacccg gcgaacccgc tgtcctatct ggatgcaccg 5941 gagcgtctga atccgcaatt tctggaggac ttcaaactgg tgcatcataa tgagccgtcc 6001 ttgatgtcca cgggcgtttt gttgtgcggc gtggagcgtc gcggtctggt tcacgcgctg 6061 cgcgataagt gccgcagcca aggcattgct attcgtttcg aaagcccgtt gctggaacac 6121 ggtgagctgc cgctggcgga ctatgatctg gtggtcctgg ctaatggtgt taatcacaaa 6181 accgcgcatt tcaccgaggc tctggtcccg caggtggact acggccgcaa taagtacatt 6241 tggtatggca ctagccagct gttcgatcag atgaatctgg tttttcgtac ccatggtaaa 6301 gatatcttta tcgcgcatgc ctataagtat agcgatacca tgagcacgtt cattgtcgaa 6361 tgtagcgaag agacttacgc acgcgcacgc ctgggcgaaa tgtccgaaga ggcgagcgca 6421 gaatacgttg ctaaggtgtt ccaggccgag ctgggtggtc acggcctggt gagccagccg 6481 ggtctgggtt ggcgtaactt catgacgttg tctcatgacc gttgtcatga tggtaagttg 6541 gttctgctgg gtgacgcgct gcaaagcggt cactttagca tcggccacgg caccacgatg 6601 gccgtggtgg tggcgcagct gctggttaaa gcgctgtgta ccgaagatgg tgtgcctgcc 6661 gcgctgaaac gtttcgaaga gcgtgccctg ccgctggtgc agttgttccg tggccacgca 6721 gacaacagcc gcgtttggtt cgaaaccgtc gaagagcgca tgcacctgtc ctcggcggaa 6781 tttgtgcaaa gcttcgacgc acgccgcaaa agcctgccgc cgatgccgga agcactggcg 6841 cagaatctgc gttatgcttt gcagcgctga tgatcattaa ggaggtaaaa aaaatggaga 6901 accgtgagcc accactgttg ccagcccgtt ggagcagcgc ctatgtctct tattggagcc 6961 cgatgctgcc ggatgaccag ctgaccagcg gctattgctg gttcgactat gaacgtgaca 7021 tctgtcgtat tgacggcctg ttcaatccgt ggagcgagcg tgatactggt tatcgcctgt 7081 ggatgtcgga ggttggtaat gcggccagcg gccgtacctg gaaacaaaaa gtcgcctatg 7141 gtcgtgagcg taccgccctg ggtgaacagc tgtgtgagcg tccgctggat gatgagactg 7201 gcccttttgc cgaattgttc ctgccacgcg atgtcctgcg ccgtctgggt gcccgtcaca 7261 ttggccgtcg cgtggttctg ggtcgcgaag cggacggttg gcgttaccag cgcccaggta 7321 aaggtccgag caccctgtac ctggatgcgg cgagcggcac tccactgcgc atggtcaccg 7381 gcgatgaagc gtcgcgtgca agcctgcgtg attttccgaa tgtgagcgag gcggagatcc 7441 cggacgcggt tttcgcggcc aagcgctaa //