BBa_M33351 1 BBa_M33351 Ptod promoter activated by TodT protein 2010-06-05T11:00:00Z 2015-05-08T01:14:01Z The sequence of the Ptod promoter was given in the following article. Lacal, J., Guazzaroni, M. E., Busch, A., Krell, T. & Ramos, J. L. (2008). Hierarchical binding of the TodT response regulator to its multiple recognition sites at the tod pathway operon promoter. J. Mol. Biol. 376, 325???337. This identical sequence was found upstream of the todX gene sequence natural existent in Pseudomonas putida given at http://www.ncbi.nlm.nih.gov/nuccore/AB042508.1 (todX coordinates: 14325..15686). Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. The catabolic genes of the toluene dioxygenase pathway are transcribed from a single promoter termed Ptod, located upstream from the todX gene. The TodS/TodT TCS mediate Ptod in response to toluene and other aromatic hydrocarbons. Ptod is activated once the response regulator TodT is phosphorylated by the TodS sensor kinase in response to pathway substrates. This part should be used in conjunction with false false _577_ 0 6929 9 Not in stock false We added restriction digestion sites to ensure compliance with Assembly Standard 10. We also modified the stop codon to TAA. false Sunny Kim annotation2070202 1 pTod promoter range2070202 1 1 116 BBa_M33355 1 BBa_M33355 pTod + Purple Color Generator 2010-06-05T11:00:00Z 2015-05-08T01:14:02Z The sequence of the Ptod promoter was given in the article referenced below. This identicle sequence was found upstream of the todX gene sequence natural existent in Pseudomonas putida given in http://www.ncbi.nlm.nih.gov/nuccore/AB042508.1 (todX coordinates: 14325..15686). Pseudomonas putida possesses a two-component regulatory system (TCS) which can sense changes in the toluene level of the environment. TodS and TodT proteins control the expression of genes involved in the degradation of toluene, benzene, and ethylbenzene via the toluene dioxygenase pathway. The catabolic genes of the toluene dioxygenase pathway are transcribed from a single promoter termed pTod, located upstream from the todX gene. The TodS/TodT TCS mediate pTod in response to toluene and other aromatic hydrocarbons. Once TodT is activated, it binds to a site on the pTod promoter approximately 107 base pairs upstream from the RNA polymerase-binding site. DNA bending caused by integration host factor (IHF) enables contact between TodT and RNA polymerase, initiating transcription. This part should be used in conjunction with BBa_M33350, the CDS for TodS downstream of an RBS, and BBa_M33354, the CDS for TodT downstream of an RBS. false false _577_ 0 6925 9 Not in stock false None false Alejandro Virrueta component2070261 1 BBa_K274002 component2070250 1 BBa_M33351 annotation2070261 1 BBa_K274002 range2070261 1 125 7469 annotation2070250 1 BBa_M33351 range2070250 1 1 116 BBa_K274002 1 BBa_K274002 Vio operon 2009-10-07T11:00:00Z 2015-05-08T01:11:45Z The VioA-E genes were originally from from Chromobacterium voilaceum ATCC 12472 in the pPSX vio+ plasmid. This was kindly provided by John Pemberton; Department of Microbiology and parasitology, University of Queensland, Brisbane, Australia. (Sarovich & Pemberton (2007) Plasmid 57:306-313). The codons were optimised for E. coli by DNA2.0 (Welch, Villalobos, Gustafsson and Minshull (2009) J. R. Soc. Interface) Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites. false false _374_ 0 5412 9 It's complicated true We added complementary restriction sites on either side of vioC and vioD (BamHI, BglII, HindIII). This allows them to be seperately removed to produce more colours from the operon. false Shuna Gould annotation2032720 1 rbs range2032720 1 1275 1291 annotation2032821 1 rbs range2032821 1 4295 4311 annotation2032552 1 rbs range2032552 1 1 17 annotation2032825 1 rbs range2032825 1 6753 6769 annotation2032668 1 vioA range2032668 1 18 1274 annotation2032824 1 vioD range2032824 1 5625 6752 annotation2032822 1 vioC range2032822 1 4312 5607 annotation2032721 1 vioB range2032721 1 1292 4294 annotation2032823 1 rbs range2032823 1 5608 5624 annotation2032826 1 vioE range2032826 1 6770 7345 BBa_M33351_sequence 1 ggcataaaccatcgtttatcacagttaaactttggttttctaagttgcgatagccatataaacccataagccaaaaaacaatatttcccagggcgtgattgtaatactgtgcgtgc BBa_M33355_sequence 1 ggcataaaccatcgtttatcacagttaaactttggttttctaagttgcgatagccatataaacccataagccaaaaaacaatatttcccagggcgtgattgtaatactgtgcgtgctactagagttaaggaggtaaaaaaaatgaaacattcttccgatatctgcattgttggtgctggtatttctggtttgacgtgcgcaagccatctgctggacagcccggcatgccgtggtctgagcctgcgtatctttgacatgcagcaagaagccggtggccgtatccgcagcaaaatgctggatggtaaggcaagcattgaactgggcgcaggtcgctactcccctcagttgcacccgcatttccaaagcgcaatgcagcactatagccaaaagagcgaagtctatccgttcacccagttgaagttcaaatctcacgtgcagcaaaagctgaagcgcgccatgaatgaactgtccccgcgtctgaaagagcatggtaaagagagctttttgcagtttgtcagccgttatcaaggtcacgatagcgcggttggtatgatccgctctatgggttacgacgcactgttcctgccggatatcagcgcagaaatggcctacgacattgtgggtaagcacccggagatccagagcgtgacggacaacgacgcgaaccaatggtttgcagcggaaacgggctttgctggtctgattcagggcatcaaggctaaggttaaggcggcaggtgcgcgttttagcctgggttatcgtctgctgagcgtccgtaccgacggtgacggctacctgctgcaactggcaggtgacgacggctggaaactggagcaccgtacccgccatctgattctggcgattccgccgagcgcgatggcgggtttgaatgttgattttccagaagcctggtccggtgcgcgctatggcagcctgccgctgtttaagggctttctgacgtacggtgagccgtggtggttggactacaaactggacgatcaggtgctgattgttgacaacccgctgcgcaaaatctatttcaaaggcgataagtacctgttcttctataccgatagcgagatggcgaattactggcgcggttgtgtcgcggagggcgaggacggttacctggagcaaattcgcacccatttggctagcgcactgggtatcgtccgtgaacgtatcccgcaaccgctggcacacgttcacaagtattgggcgcacggcgttgagttttgccgtgattctgatattgaccacccgagcgcactgtctcatcgcgacagcggtatcatcgcgtgctccgatgcgtacacggagcattgtggttggatggagggcggtctgctgagcgcccgtgaggcaagccgtctgctgttgcagcgtatcgccgcgtgattaaggaggtaaaaaaaatgagcattctggatttcccgcgtatccacttccgtggctgggcccgtgtcaatgcgccgaccgcgaaccgcgatccgcacggccacatcgatatggccagcaataccgtggcgatggcgggtgagccgttcgacctggcacgccatcctacggagttccaccgtcacctgcgctccctgggtccgcgcttcggcttggatggtcgtgctgacccggaaggcccgttcagcctggccgagggctacaacgctgccggtaacaaccacttttcgtgggagagcgcaaccgttagccacgtgcaatgggatggcggtgaggcggatcgtggtgacggtctggtcggtgctcgtttggcactgtggggtcactacaatgattatctgcgtaccaccttcaatcgtgctcgttgggtcgacagcgacccgacgcgccgtgacgctgcacaaatctatgcgggccaattcaccattagcccggctggtgccggtccgggtacgccgtggctgtttacggcagacattgatgatagccatggtgcacgttggacgcgtggcggccacattgcagagcgtggcggccacttcttggatgaagagtttggtctggcacgcctgtttcagttctctgtgccgaaagatcacccacattttctgtttcacccgggtccgtttgattccgaggcctggcgtcgtctgcaattggctctggaggatgacgacgttctgggtctgaccgtgcaatatgcgttgttcaatatgagcaccccgcctcagccgaacagcccggtttttcacgatatggtcggtgttgtcggtctgtggcgtcgtggtgaactggcgagctacccggctggtcgtctgctgcgtccgcgtcaaccgggtctgggtgacctgaccctgcgcgtcaacggtggtcgcgttgcgctgaatttggcgtgtgccattccgttcagcactcgtgccgcgcagccaagcgcaccggaccgcctgaccccggacctgggtgccaaactgccgctgggcgatctgctgctgcgtgatgaggacggcgcactgttggcacgtgtgccgcaggctctgtaccaagactattggacgaatcacggtattgtggacctgccgctgctgcgcgaaccgcgtggtagcttgaccctgagcagcgaactggcggagtggcgtgagcaagactgggtcacccaaagcgacgcgtctaacctgtacctggaggcaccggatcgccgtcacggtcgctttttccctgagagcatcgcgctgcgcagctactttcgcggtgaagcgcgtgcgcgtccggatatcccgcatcgtatcgagggcatgggcctggtcggcgtcgaatctcgtcaggatggcgacgctgcggaatggcgtctgacgggtctgcgtccgggtccggcacgcattgttctggacgatggtgccgaggcgatccctctgcgtgttctgcctgacgattgggcgctggatgacgcgaccgtcgaagaagtggattacgcctttttgtaccgccacgttatggcgtattacgagctggtgtatccattcatgagcgacaaggtgttttccctggctgatcgttgcaaatgtgaaacgtacgcacgtctgatgtggcagatgtgtgatccgcagaaccgcaacaagtcctattacatgccgagcacccgcgaactgtcggcaccgaaagctcgtttgttcttgaagtatctggcccacgtggaaggccaggcacgcctgcaagcacctccgccagcgggtccggcacgcattgaatctaaagcccagttggcggcagagctgcgtaaagccgtcgacctggagctgtctgtgatgctgcaatacctgtacgcggcgtatagcattccgaactatgcacagggccaacaacgtgttcgtgacggtgcgtggaccgccgagcagctgcaactggcgtgcggtagcggtgaccgtcgccgtgatggcggtattcgtgcagcactgctggaaattgctcatgaagaaatgattcattacctggtcgttaacaacctgctgatggccctgggcgagccgttctacgcgggtgtcccgctgatgggcgaagcggcacgtcaggcgtttggcctggacaccgagttcgctctggaaccgtttagcgaaagcacgctggcacgttttgttcgtctggaatggccgcactttatcccagcaccgggcaaatccatcgcggactgctatgccgccattcgtcaggcgtttttggatctgccggacttgtttggtggcgaggcaggtaagcgtggcggtgaacaccacctgttcctgaatgagctgaccaaccgtgcgcatccgggttatcaactggaagttttcgatcgcgactcggcgctgtttggtattgcatttgtgaccgatcagggcgaaggtggcgctctggacagcccgcactacgaacatagccattttcaacgtctgcgtgaaatgagcgcgcgtatcatggctcaaagcgcaccgttcgaaccggcgctgccggcgttgcgtaatccggttctggatgagagcccgggttgccaacgtgtcgcagacggtcgtgcgcgtgcgctgatggcattgtaccaaggcgtttatgagctgatgtttgcgatgatggcgcagcacttcgccgtgaaaccgctgggtagcttgcgtcgcagccgcctgatgaacgcagcaatcgatctgatgaccggtctgttgcgtccgctgagctgcgcgctgatgaacctgccaagcggcatcgccggtcgcacggccggtccgccgctgccgggtccggttgacacccgtagctatgacgactacgcgctgggctgtcgcatgctggcacgccgttgcgagcgtctgctggagcaggcgagcatgctggaaccgggttggctgccggatgcgcagatggagctgctggatttctatcgtcgccaaatgctggacttggcgtgcggcaaactgagccgcgaggcctaaggatccttaaggaggtaaaaaaaatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaagtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcggcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcgaaccgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccgctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagcataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaaggtgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtttcgagttccagcaaacgttcttccgccatggctacaaaaccctggttttgccggacgcgcaagcactgggttaccgtaaagacacgctgtactttttcggcatggattccggtggcctgttcgcgggtcgtgcggctacgatcccagatggtagcgtcagcatcgccgtttgcctgccgtactcgggtagcccttccctgacgaccaccgacgaaccgacgatgcgtgcgttcttcgatcgttacttcggtggcctgccgcgtgacgcgcgtgacgaaatgctgcgtcagtttctggcgaagccgagcaacgacctgattaacgtgcgctctagcacctttcactataagggtaatgtgctgttgctgggtgatgctgcgcatgcgactgcgccgttcctgggtcagggtatgaacatggcgctggaggacgcccgcacgtttgtcgagctgctggaccgccaccagggcgaccaagacaaagcctttccggagttcacggagctgcgcaaagtccaggcagacgcaatgcaagacatggctcgcgccaactatgacgttttgagctgctcgaacccgatctttttcatgcgtgcgcgttacacgcgttacatgcattccaagtttccgggcctgtatccgccggatatggccgagaaactgtactttacgagcgagccgtacgatcgtctgcaacaaatccagcgtaaacagaatgtttggtacaagattggtcgcgtgaattgaagatctttaaggaggtaaaaaaaatgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcagaatctgcgttatgctttgcagcgctgatgatcattaaggaggtaaaaaaaatggagaaccgtgagccaccactgttgccagcccgttggagcagcgcctatgtctcttattggagcccgatgctgccggatgaccagctgaccagcggctattgctggttcgactatgaacgtgacatctgtcgtattgacggcctgttcaatccgtggagcgagcgtgatactggttatcgcctgtggatgtcggaggttggtaatgcggccagcggccgtacctggaaacaaaaagtcgcctatggtcgtgagcgtaccgccctgggtgaacagctgtgtgagcgtccgctggatgatgagactggcccttttgccgaattgttcctgccacgcgatgtcctgcgccgtctgggtgcccgtcacattggccgtcgcgtggttctgggtcgcgaagcggacggttggcgttaccagcgcccaggtaaaggtccgagcaccctgtacctggatgcggcgagcggcactccactgcgcatggtcaccggcgatgaagcgtcgcgtgcaagcctgcgtgattttccgaatgtgagcgaggcggagatcccggacgcggttttcgcggccaagcgctaa BBa_K274002_sequence 1 ttaaggaggtaaaaaaaatgaaacattcttccgatatctgcattgttggtgctggtatttctggtttgacgtgcgcaagccatctgctggacagcccggcatgccgtggtctgagcctgcgtatctttgacatgcagcaagaagccggtggccgtatccgcagcaaaatgctggatggtaaggcaagcattgaactgggcgcaggtcgctactcccctcagttgcacccgcatttccaaagcgcaatgcagcactatagccaaaagagcgaagtctatccgttcacccagttgaagttcaaatctcacgtgcagcaaaagctgaagcgcgccatgaatgaactgtccccgcgtctgaaagagcatggtaaagagagctttttgcagtttgtcagccgttatcaaggtcacgatagcgcggttggtatgatccgctctatgggttacgacgcactgttcctgccggatatcagcgcagaaatggcctacgacattgtgggtaagcacccggagatccagagcgtgacggacaacgacgcgaaccaatggtttgcagcggaaacgggctttgctggtctgattcagggcatcaaggctaaggttaaggcggcaggtgcgcgttttagcctgggttatcgtctgctgagcgtccgtaccgacggtgacggctacctgctgcaactggcaggtgacgacggctggaaactggagcaccgtacccgccatctgattctggcgattccgccgagcgcgatggcgggtttgaatgttgattttccagaagcctggtccggtgcgcgctatggcagcctgccgctgtttaagggctttctgacgtacggtgagccgtggtggttggactacaaactggacgatcaggtgctgattgttgacaacccgctgcgcaaaatctatttcaaaggcgataagtacctgttcttctataccgatagcgagatggcgaattactggcgcggttgtgtcgcggagggcgaggacggttacctggagcaaattcgcacccatttggctagcgcactgggtatcgtccgtgaacgtatcccgcaaccgctggcacacgttcacaagtattgggcgcacggcgttgagttttgccgtgattctgatattgaccacccgagcgcactgtctcatcgcgacagcggtatcatcgcgtgctccgatgcgtacacggagcattgtggttggatggagggcggtctgctgagcgcccgtgaggcaagccgtctgctgttgcagcgtatcgccgcgtgattaaggaggtaaaaaaaatgagcattctggatttcccgcgtatccacttccgtggctgggcccgtgtcaatgcgccgaccgcgaaccgcgatccgcacggccacatcgatatggccagcaataccgtggcgatggcgggtgagccgttcgacctggcacgccatcctacggagttccaccgtcacctgcgctccctgggtccgcgcttcggcttggatggtcgtgctgacccggaaggcccgttcagcctggccgagggctacaacgctgccggtaacaaccacttttcgtgggagagcgcaaccgttagccacgtgcaatgggatggcggtgaggcggatcgtggtgacggtctggtcggtgctcgtttggcactgtggggtcactacaatgattatctgcgtaccaccttcaatcgtgctcgttgggtcgacagcgacccgacgcgccgtgacgctgcacaaatctatgcgggccaattcaccattagcccggctggtgccggtccgggtacgccgtggctgtttacggcagacattgatgatagccatggtgcacgttggacgcgtggcggccacattgcagagcgtggcggccacttcttggatgaagagtttggtctggcacgcctgtttcagttctctgtgccgaaagatcacccacattttctgtttcacccgggtccgtttgattccgaggcctggcgtcgtctgcaattggctctggaggatgacgacgttctgggtctgaccgtgcaatatgcgttgttcaatatgagcaccccgcctcagccgaacagcccggtttttcacgatatggtcggtgttgtcggtctgtggcgtcgtggtgaactggcgagctacccggctggtcgtctgctgcgtccgcgtcaaccgggtctgggtgacctgaccctgcgcgtcaacggtggtcgcgttgcgctgaatttggcgtgtgccattccgttcagcactcgtgccgcgcagccaagcgcaccggaccgcctgaccccggacctgggtgccaaactgccgctgggcgatctgctgctgcgtgatgaggacggcgcactgttggcacgtgtgccgcaggctctgtaccaagactattggacgaatcacggtattgtggacctgccgctgctgcgcgaaccgcgtggtagcttgaccctgagcagcgaactggcggagtggcgtgagcaagactgggtcacccaaagcgacgcgtctaacctgtacctggaggcaccggatcgccgtcacggtcgctttttccctgagagcatcgcgctgcgcagctactttcgcggtgaagcgcgtgcgcgtccggatatcccgcatcgtatcgagggcatgggcctggtcggcgtcgaatctcgtcaggatggcgacgctgcggaatggcgtctgacgggtctgcgtccgggtccggcacgcattgttctggacgatggtgccgaggcgatccctctgcgtgttctgcctgacgattgggcgctggatgacgcgaccgtcgaagaagtggattacgcctttttgtaccgccacgttatggcgtattacgagctggtgtatccattcatgagcgacaaggtgttttccctggctgatcgttgcaaatgtgaaacgtacgcacgtctgatgtggcagatgtgtgatccgcagaaccgcaacaagtcctattacatgccgagcacccgcgaactgtcggcaccgaaagctcgtttgttcttgaagtatctggcccacgtggaaggccaggcacgcctgcaagcacctccgccagcgggtccggcacgcattgaatctaaagcccagttggcggcagagctgcgtaaagccgtcgacctggagctgtctgtgatgctgcaatacctgtacgcggcgtatagcattccgaactatgcacagggccaacaacgtgttcgtgacggtgcgtggaccgccgagcagctgcaactggcgtgcggtagcggtgaccgtcgccgtgatggcggtattcgtgcagcactgctggaaattgctcatgaagaaatgattcattacctggtcgttaacaacctgctgatggccctgggcgagccgttctacgcgggtgtcccgctgatgggcgaagcggcacgtcaggcgtttggcctggacaccgagttcgctctggaaccgtttagcgaaagcacgctggcacgttttgttcgtctggaatggccgcactttatcccagcaccgggcaaatccatcgcggactgctatgccgccattcgtcaggcgtttttggatctgccggacttgtttggtggcgaggcaggtaagcgtggcggtgaacaccacctgttcctgaatgagctgaccaaccgtgcgcatccgggttatcaactggaagttttcgatcgcgactcggcgctgtttggtattgcatttgtgaccgatcagggcgaaggtggcgctctggacagcccgcactacgaacatagccattttcaacgtctgcgtgaaatgagcgcgcgtatcatggctcaaagcgcaccgttcgaaccggcgctgccggcgttgcgtaatccggttctggatgagagcccgggttgccaacgtgtcgcagacggtcgtgcgcgtgcgctgatggcattgtaccaaggcgtttatgagctgatgtttgcgatgatggcgcagcacttcgccgtgaaaccgctgggtagcttgcgtcgcagccgcctgatgaacgcagcaatcgatctgatgaccggtctgttgcgtccgctgagctgcgcgctgatgaacctgccaagcggcatcgccggtcgcacggccggtccgccgctgccgggtccggttgacacccgtagctatgacgactacgcgctgggctgtcgcatgctggcacgccgttgcgagcgtctgctggagcaggcgagcatgctggaaccgggttggctgccggatgcgcagatggagctgctggatttctatcgtcgccaaatgctggacttggcgtgcggcaaactgagccgcgaggcctaaggatccttaaggaggtaaaaaaaatgaaacgtgcgattatcgttggtggcggcctggcgggtggcctgaccgcgatctacctggcgaagcgtggctacgaagtgcacgtcgtggagaagcgtggtgatcctctgcgcgatctgagctcttacgtggacgttgttagcagccgtgcgatcggcgtgagcatgaccgttcgtggtatcaagagcgttttggctgcgggcattccgcgtgcagagctggatgcgtgtggcgaaccgatcgtggcaatggctttctccgtgggtggtcagtatcgcatgcgcgaactgaagccgttggaggatttccgtccgctgagcttgaaccgtgcggcgtttcaaaagctgctgaacaaatacgcgaacctggcaggcgttcgttactactttgagcataagtgcctggatgttgacctggatggtaagagcgtgttgattcagggcaaagatggtcagccgcagcgtctgcaaggtgacatgattatcggtgcggatggcgcccacagcgccgtccgtcaggcgatgcagagcggcctgcgtcgtttcgagttccagcaaacgttcttccgccatggctacaaaaccctggttttgccggacgcgcaagcactgggttaccgtaaagacacgctgtactttttcggcatggattccggtggcctgttcgcgggtcgtgcggctacgatcccagatggtagcgtcagcatcgccgtttgcctgccgtactcgggtagcccttccctgacgaccaccgacgaaccgacgatgcgtgcgttcttcgatcgttacttcggtggcctgccgcgtgacgcgcgtgacgaaatgctgcgtcagtttctggcgaagccgagcaacgacctgattaacgtgcgctctagcacctttcactataagggtaatgtgctgttgctgggtgatgctgcgcatgcgactgcgccgttcctgggtcagggtatgaacatggcgctggaggacgcccgcacgtttgtcgagctgctggaccgccaccagggcgaccaagacaaagcctttccggagttcacggagctgcgcaaagtccaggcagacgcaatgcaagacatggctcgcgccaactatgacgttttgagctgctcgaacccgatctttttcatgcgtgcgcgttacacgcgttacatgcattccaagtttccgggcctgtatccgccggatatggccgagaaactgtactttacgagcgagccgtacgatcgtctgcaacaaatccagcgtaaacagaatgtttggtacaagattggtcgcgtgaattgaagatctttaaggaggtaaaaaaaatgaagattctggtcattggtgctggtccagctggtctggttttcgcatcccaactgaagcaggcacgccctttgtgggccattgacatcgtggagaagaatgacgagcaagaagtgctgggctggggtgtcgtgctgcctggccgtccgggtcagcacccggcgaacccgctgtcctatctggatgcaccggagcgtctgaatccgcaatttctggaggacttcaaactggtgcatcataatgagccgtccttgatgtccacgggcgttttgttgtgcggcgtggagcgtcgcggtctggttcacgcgctgcgcgataagtgccgcagccaaggcattgctattcgtttcgaaagcccgttgctggaacacggtgagctgccgctggcggactatgatctggtggtcctggctaatggtgttaatcacaaaaccgcgcatttcaccgaggctctggtcccgcaggtggactacggccgcaataagtacatttggtatggcactagccagctgttcgatcagatgaatctggtttttcgtacccatggtaaagatatctttatcgcgcatgcctataagtatagcgataccatgagcacgttcattgtcgaatgtagcgaagagacttacgcacgcgcacgcctgggcgaaatgtccgaagaggcgagcgcagaatacgttgctaaggtgttccaggccgagctgggtggtcacggcctggtgagccagccgggtctgggttggcgtaacttcatgacgttgtctcatgaccgttgtcatgatggtaagttggttctgctgggtgacgcgctgcaaagcggtcactttagcatcggccacggcaccacgatggccgtggtggtggcgcagctgctggttaaagcgctgtgtaccgaagatggtgtgcctgccgcgctgaaacgtttcgaagagcgtgccctgccgctggtgcagttgttccgtggccacgcagacaacagccgcgtttggttcgaaaccgtcgaagagcgcatgcacctgtcctcggcggaatttgtgcaaagcttcgacgcacgccgcaaaagcctgccgccgatgccggaagcactggcgcagaatctgcgttatgctttgcagcgctgatgatcattaaggaggtaaaaaaaatggagaaccgtgagccaccactgttgccagcccgttggagcagcgcctatgtctcttattggagcccgatgctgccggatgaccagctgaccagcggctattgctggttcgactatgaacgtgacatctgtcgtattgacggcctgttcaatccgtggagcgagcgtgatactggttatcgcctgtggatgtcggaggttggtaatgcggccagcggccgtacctggaaacaaaaagtcgcctatggtcgtgagcgtaccgccctgggtgaacagctgtgtgagcgtccgctggatgatgagactggcccttttgccgaattgttcctgccacgcgatgtcctgcgccgtctgggtgcccgtcacattggccgtcgcgtggttctgggtcgcgaagcggacggttggcgttaccagcgcccaggtaaaggtccgagcaccctgtacctggatgcggcgagcggcactccactgcgcatggtcaccggcgatgaagcgtcgcgtgcaagcctgcgtgattttccgaatgtgagcgaggcggagatcccggacgcggttttcgcggccaagcgctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z