This part combines the arsenic transport genes and the photochelatin synthase gene from Arabadopsis Thaliana. Coupled with the Arsenic resistance pathway, this part is designed to complex and transport arsenic into a vacuole.
The largest issue is the promoter being used- it really should be a promoter that is designed to work in plants. Finding a promoter that works in plants, but is sensitive to arsenic concentrations has almost impossible. The same can be said about the RBS and terminating sequences- ideally, these would be plant specific as well.
Shambhu Koirala, Nathan Kipniss, Tyler Wagner
From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
Coding Sequence for Phytochelatin Synthase found in Arabidopsis thaliana.
Arabidopsis Thaliana, http://www.ncbi.nlm.nih.gov/nuccore/AF085231.1
Polychelatins are a polymer of glutathione that are used stress responses to toxins. The phytochelatins bind to the toxins (commonly heavy metal toxins like Arsenic) and are then transported in to the vacuole in order to isolate the toxins from important components in the cytosol such as vital proteins and organelles.
Unwanted restriction sites had to be removed.
Nathan Kipniss, Tyler Wagner, Shambhu Koirala
Coding Sequence for Arsenic Transport 1 found in Arabadopsis Thaliana
Arabadopsis Thaliana http://www.ncbi.nlm.nih.gov/nuccore/NM_102777.2
This part takes an arsenic-phytochelatin complex and transports it into the vacuole. That way, arsenic does not interfere with important processes in the cell (like glycolysis, which arsenic can shut down).
Coding Sequence for Arsenic Transport 2, from Arabadopsis Thaliana
This part codes for a second transporter which takes phytochelatin-arsenic complexes into the vacuole.
Nathan Kipniss, Shambhu Koirala, Tyler Wagner
eukaryotic -- derived from SV40 early poly A signal sequence
It is cloned in BioBrick vector pSB1AK3.
consensus Kozak and start built from oligonucleotides
consensus Kozak sequence and start codon
designed such that fusion using fusion bricks assembly method leads to in frame translation
Conversion of the iGEM parts registry to SBOL2.1