BBa_M34576
type: ComponentDefinition
persistent identity: BBa M34576
display id: BBa_M34576
version: 1
was generated by: iGEM to SBOL conversion
description: Arsenic Transport
created: 2011-05-08T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Arabadopsis Thaliana
owned by: james
owned by: myers
mutable description: This part combines the arsenic transport genes and the photochelatin synthase gene from Arabadopsis Thaliana. Coupled with the Arsenic resistance pathway, this part is designed to complex and transport arsenic into a vacuole.
discontinued: false
dominant: false
experience: experience/None
group u list: _838_
m user id: 0
owner id: 9444
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The largest issue is the promoter being used- it really should be a promoter that is designed to work in plants. Finding a promoter that works in plants, but is sensitive to arsenic concentrations has almost impossible. The same can be said about the RBS and terminating sequences- ideally, these would be plant specific as well.
star: false
creator: Shambhu Koirala, Nathan Kipniss, Tyler Wagner
type: DnaRegion
role: partType/Coding
role: SO:0000316
component: BBa_M34572
component: BBa_J63003
component: BBa_M34569
component: BBa_J52016
component: BBa_M34573
component: BBa_K165000
sequence annotation: BBa_K165000
sequence annotation: BBa_J63003
sequence annotation: BBa_M34569
sequence annotation: BBa_M34572
sequence annotation: BBa_M34573
sequence annotation: BBa_J52016
sequence: https://synbiohub.org/public/igem/BBa_M34576_sequence/1

BBa_M34572
type: Component
persistent identity: component2119788
display id: component2119788
version: 1
top level: BBa_M34576
definition: BBa_M34572
access: public

BBa_J63003
type: Component
persistent identity: component2119787
display id: component2119787
version: 1
top level: BBa_M34576
definition: Kozak & st
access: public

BBa_M34569
type: Component
persistent identity: component2119785
display id: component2119785
version: 1
top level: BBa_M34576
definition: BBa_M34569
access: public

BBa_J63003
type: Component
persistent identity: component2119784
display id: component2119784
version: 1
top level: BBa_M34576
definition: Kozak & st
access: public

BBa_J52016
type: Component
persistent identity: component2119794
display id: component2119794
version: 1
top level: BBa_M34576
definition: BBa_J52016
access: public

BBa_M34573
type: Component
persistent identity: component2119791
display id: component2119791
version: 1
top level: BBa_M34576
definition: BBa_M34573
access: public

BBa_J63003
type: Component
persistent identity: component2119790
display id: component2119790
version: 1
top level: BBa_M34576
definition: Kozak & st
access: public

BBa_K165000
type: Component
persistent identity: component2119782
display id: component2119782
version: 1
top level: BBa_M34576
definition: BBa_K165000
access: public

BBa_K165000
type: SequenceAnnotation
persistent identity: annotation2119782
display id: annotation2119782
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119782/range2119782/1
component: BBa_K165000

https://synbiohub.org/public/igem/BBa_M34576/annotation2119782/range2119782/1
type: Range
persistent identity: range2119782
display id: range2119782
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 1
end: 387
orientation: inline

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2119784
display id: annotation2119784
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119784/range2119784/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_M34576/annotation2119784/range2119784/1
type: Range
persistent identity: range2119784
display id: range2119784
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 396
end: 413
orientation: inline

BBa_M34569
type: SequenceAnnotation
persistent identity: annotation2119785
display id: annotation2119785
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119785/range2119785/1
component: BBa_M34569

https://synbiohub.org/public/igem/BBa_M34576/annotation2119785/range2119785/1
type: Range
persistent identity: range2119785
display id: range2119785
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 422
end: 3579
orientation: inline

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2119787
display id: annotation2119787
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119787/range2119787/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_M34576/annotation2119787/range2119787/1
type: Range
persistent identity: range2119787
display id: range2119787
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 3588
end: 3605
orientation: inline

BBa_M34572
type: SequenceAnnotation
persistent identity: annotation2119788
display id: annotation2119788
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119788/range2119788/1
component: BBa_M34572

https://synbiohub.org/public/igem/BBa_M34576/annotation2119788/range2119788/1
type: Range
persistent identity: range2119788
display id: range2119788
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 3614
end: 8781
orientation: inline

BBa_J63003
type: SequenceAnnotation
persistent identity: annotation2119790
display id: annotation2119790
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119790/range2119790/1
component: BBa_J63003

https://synbiohub.org/public/igem/BBa_M34576/annotation2119790/range2119790/1
type: Range
persistent identity: range2119790
display id: range2119790
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 8790
end: 8807
orientation: inline

BBa_M34573
type: SequenceAnnotation
persistent identity: annotation2119791
display id: annotation2119791
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119791/range2119791/1
component: BBa_M34573

https://synbiohub.org/public/igem/BBa_M34576/annotation2119791/range2119791/1
type: Range
persistent identity: range2119791
display id: range2119791
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 8816
end: 14432
orientation: inline

BBa_J52016
type: SequenceAnnotation
persistent identity: annotation2119794
display id: annotation2119794
version: 1
top level: BBa_M34576
location: https://synbiohub.org/public/igem/BBa_M34576/annotation2119794/range2119794/1
component: BBa_J52016

https://synbiohub.org/public/igem/BBa_M34576/annotation2119794/range2119794/1
type: Range
persistent identity: range2119794
display id: range2119794
version: 1
top level: BBa_M34576
direction: direction/reverse
start: 14441
end: 14678
orientation: inline

BBa_K165000
type: ComponentDefinition
persistent identity: BBa K165000
display id: BBa_K165000
version: 1
was generated by: iGEM to SBOL conversion
description: MET 25 Promoter
created: 2008-10-24T11:00:00Z
modified: 2015-05-08T01:10:55Z
mutable provenance: From the Saccharomyces cerevisiae genome, by way of Caroline Ajo-Franklin.
owned by: james
owned by: myers
mutable description: The is a repressable promoter for use in Saccharomyces cerevisiae. It is repressed by the presence of methionine. When unrepresssed, is has a moderate level of activity.
discontinued: false
dominant: false
experience: experience/Works
group u list: _267_
m user id: 0
owner id: 2512
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: John Szymanski
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K165000_sequence/1

BBa_M34569
type: ComponentDefinition
persistent identity: BBa M34569
display id: BBa_M34569
version: 1
was generated by: iGEM to SBOL conversion
description: Coding Sequence for Phytochelatin Synthase found in Arabidopsis thaliana.
created: 2011-05-08T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Arabidopsis Thaliana, http://www.ncbi.nlm.nih.gov/nuccore/AF085231.1
owned by: james
owned by: myers
mutable description: Polychelatins are a polymer of glutathione that are used stress responses to toxins. The phytochelatins bind to the toxins (commonly heavy metal toxins like Arsenic) and are then transported in to the vacuole in order to isolate the toxins from important components in the cytosol such as vital proteins and organelles.
discontinued: false
dominant: false
experience: experience/None
group u list: _838_
m user id: 0
owner id: 9444
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Unwanted restriction sites had to be removed.
star: false
creator: Nathan Kipniss, Tyler Wagner, Shambhu Koirala
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M34569_sequence/1

BBa_M34572
type: ComponentDefinition
persistent identity: BBa M34572
display id: BBa_M34572
version: 1
was generated by: iGEM to SBOL conversion
description: Coding Sequence for Arsenic Transport 1 found in Arabadopsis Thaliana
created: 2011-05-08T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Arabadopsis Thaliana http://www.ncbi.nlm.nih.gov/nuccore/NM_102777.2
owned by: james
owned by: myers
mutable description: This part takes an arsenic-phytochelatin complex and transports it into the vacuole. That way, arsenic does not interfere with important processes in the cell (like glycolysis, which arsenic can shut down).
discontinued: false
dominant: false
experience: experience/None
group u list: _838_
m user id: 0
owner id: 9444
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Unwanted restriction sites had to be removed.
star: false
creator: Nathan Kipniss, Tyler Wagner, Shambhu Koirala
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_M34572_sequence/1

BBa_M34573
type: ComponentDefinition
persistent identity: BBa M34573
display id: BBa_M34573
version: 1
was generated by: iGEM to SBOL conversion
description: Coding Sequence for Arsenic Transport 2, from Arabadopsis Thaliana
created: 2011-05-08T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: http://www.ncbi.nlm.nih.gov/nuccore/NM_129020.3
owned by: james
owned by: myers
mutable description: This part codes for a second transporter which takes phytochelatin-arsenic complexes into the vacuole.
discontinued: false
dominant: false
experience: experience/None
group u list: _838_
m user id: 0
owner id: 9444
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Unwanted restriction sites had to be removed.
star: false
creator: Nathan Kipniss, Shambhu Koirala, Tyler Wagner
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_M34573_sequence/1

BBa_J52016
type: ComponentDefinition
persistent identity: BBa J52016
display id: BBa_J52016
version: 1
was generated by: iGEM to SBOL conversion
description: eukaryotic -- derived from SV40 early poly A signal sequence
created: 2006-10-13T11:00:00Z
modified: 2015-08-31T03:54:04Z
mutable provenance: -
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _80_
m user id: 0
owner id: 800
owning group id: 80
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: It is cloned in BioBrick vector pSB1AK3.
star: false
creator: Monika Ciglic
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_J52016_sequence/1

terminator
type: SequenceAnnotation
persistent identity: annotation1902869
display id: annotation1902869
version: 1
top level: BBa_J52016
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J52016/annotation1902869/range1902869/1
type: Range
persistent identity: range1902869
display id: range1902869
version: 1
top level: BBa_J52016
direction: direction/forward
start: 1
end: 238
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation1902870
display id: annotation1902870
version: 1
top level: BBa_J52016
location: https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J52016/annotation1902870/range1902870/1
type: Range
persistent identity: range1902870
display id: range1902870
version: 1
top level: BBa_J52016
direction: direction/forward
start: 1
end: 238
orientation: inline

Kozak & st
type: ComponentDefinition
persistent identity: BBa J63003
display id: BBa_J63003
version: 1
was generated by: iGEM to SBOL conversion
description: designed yeast Kozak sequence
created: 2006-10-10T11:00:00Z
modified: 2015-08-31T01:56:26Z
mutable provenance: consensus Kozak and start built from oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: consensus Kozak sequence and start codon
discontinued: false
dominant: false
experience: experience/Works
group u list: _97_
m user id: 0
owner id: 545
owning group id: 97
sample status: In stock
status: status/Available
bookmark: false
mutable notes: designed such that fusion using fusion bricks assembly method leads to in frame translation
star: true
creator: Caroline Ajo-Franklin
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: start codon
sequence: https://synbiohub.org/public/igem/BBa_J63003_sequence/1

start codon
type: SequenceAnnotation
persistent identity: annotation1902852
display id: annotation1902852
version: 1
top level: Kozak & st
location: https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_J63003/annotation1902852/range1902852/1
type: Range
persistent identity: range1902852
display id: range1902852
version: 1
top level: Kozak & st
direction: direction/forward
start: 13
end: 15
orientation: inline

https://synbiohub.org/public/igem/BBa_M34576_sequence/1
type: Sequence
persistent identity: BBa M34576 sequence
display id: BBa_M34576_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatactactagagcccgccgccaccatggagtactagagttattttcgaatccactaacgaatcttccacagcaaacacttttgtgttcctctgtaatttctcagtatatatagataccaaaacaagcagtgaaaaatggctatggcgagtttatatcggcgatctcttccttctcctccggccattgacttttcttccgccgaaggcaaggtacgtacggatcaagtttcgatttcatggattgcgattataaactaggtttttggttacgcgaatttttctgtggttctgatgaatcacgatctccagactccaatctcgatttcgtttttgtttttgtcttcaatactcatgagatcttacaattcgttcagaacttgaatcaaacgcgttttgtaaaattagatactggatttggatttgaattgtgaaaacacataatgtagtcgttgacagtgatcatcacttgtgaaccttctaaggtcagtgacagtgatcacagagatctgaatatatctccaagtaatttcagtgatagtgtttctggaagtagtacgcttggtggttatatttactggagatgtacttgttaatgttagctgtgttgatgtctaaaaagtatttttgatcatttgtggcagctaatcttcaatgaagcgcttcagaaaggaactatggaaggatttttcaggttgatttcgtattttcagacacaatccgaacctgcgtattgtggtttggctagtctctcagtggtgttgaatgctctttctatcgatcctggacgtaaatggaaaggtatgcaagattgaagcaaattatgtccacactaaataaatgatggttttttataaaaagttttcctctcttccaatagggccttggaggtggtttgatgaatcaatgttggattgctgcgaacctctggaagtagtgaaggaaaaaggcatttcatttggaaaagttgtctgtttggctcattgttcaggagcaaaagttgaggctttccgtacaagtcagagcaccattgatgatttccgcaaatttgtcgtcaaatgcacgagttctgagaattgtcatatgatctcaacatatcaccgaggtgtatttaagcaggtaaagcataactttgtacacactgttttgctaaggctcagtcatattttcaagttttaaaacgtctggtggctataattgcagactgggactggtcacttttcacctattggtggctataatgctgagagagatatggctttgattcttgatgttgctcgtttcaagtatccccctcactgggttcctcttaaacttctttgggaagccatggacagtattgatcagtcaacagggaaacgtagagggtacataatcttttcttatctttcgcttaggttcttactgtatcatggatgtaatatttctatataatatggtctgatttttttctgtgttgttctcaggttcatgctcatatctgaaccacacagagaacccggattgctctatactctggtatgttcgatgcaatataggttctctttaagtttgctaatatctttttcttcaattttgctattctattaatgcaattttctcactcacaatctcttccac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K165000_sequence/1
type: Sequence
persistent identity: BBa K165000 sequence
display id: BBa_K165000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctccggatgcaagggttcgaatcccttagctctcattattttttgctttttctcttgaggtcacatgatcgcaaaatggcaaatggcacgtgaagctgtcgatattggggaactgtggtggttggcaaatgactaattaagttagtcaaggcgccatcctcatgaaaactgtgtaacataataaccgaagtgtcgaaaaggtggcaccttgtccaattgaacacgctcgatgaaaaaaataagatatatataaggttaagtaaagcgtctgttagaaaggaagtttttcctttttcttgctctcttgtcttttcatctactatttccttcgtgtaatacagggtcgtcagatacatagatacaattctattacccccatccatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M34569_sequence/1
type: Sequence
persistent identity: BBa M34569 sequence
display id: BBa_M34569_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattttcgaatccactaacgaatcttccacagcaaacacttttgtgttcctctgtaatttctcagtatatatagataccaaaacaagcagtgaaaaatggctatggcgagtttatatcggcgatctcttccttctcctccggccattgacttttcttccgccgaaggcaaggtacgtacggatcaagtttcgatttcatggattgcgattataaactaggtttttggttacgcgaatttttctgtggttctgatgaatcacgatctccagactccaatctcgatttcgtttttgtttttgtcttcaatactcatgagatcttacaattcgttcagaacttgaatcaaacgcgttttgtaaaattagatactggatttggatttgaattgtgaaaacacataatgtagtcgttgacagtgatcatcacttgtgaaccttctaaggtcagtgacagtgatcacagagatctgaatatatctccaagtaatttcagtgatagtgtttctggaagtagtacgcttggtggttatatttactggagatgtacttgttaatgttagctgtgttgatgtctaaaaagtatttttgatcatttgtggcagctaatcttcaatgaagcgcttcagaaaggaactatggaaggatttttcaggttgatttcgtattttcagacacaatccgaacctgcgtattgtggtttggctagtctctcagtggtgttgaatgctctttctatcgatcctggacgtaaatggaaaggtatgcaagattgaagcaaattatgtccacactaaataaatgatggttttttataaaaagttttcctctcttccaatagggccttggaggtggtttgatgaatcaatgttggattgctgcgaacctctggaagtagtgaaggaaaaaggcatttcatttggaaaagttgtctgtttggctcattgttcaggagcaaaagttgaggctttccgtacaagtcagagcaccattgatgatttccgcaaatttgtcgtcaaatgcacgagttctgagaattgtcatatgatctcaacatatcaccgaggtgtatttaagcaggtaaagcataactttgtacacactgttttgctaaggctcagtcatattttcaagttttaaaacgtctggtggctataattgcagactgggactggtcacttttcacctattggtggctataatgctgagagagatatggctttgattcttgatgttgctcgtttcaagtatccccctcactgggttcctcttaaacttctttgggaagccatggacagtattgatcagtcaacagggaaacgtagagggtacataatcttttcttatctttcgcttaggttcttactgtatcatggatgtaatatttctatataatatggtctgatttttttctgtgttgttctcaggttcatgctcatatctgaaccacacagagaacccggattgctctatactctggtatgttcgatgcaatataggttctctttaagtttgctaatatctttttcttcaattttgctattctattaatgcaattttctcactcacaatctcttccacatgtattgaatgaacttgagatccttatgagcattttggtaagacaaaacttgtggattggtgataattttgcttttatatatctataccagagctgcaaggatgaaagctggatcgaaatagccaagtatttgaaggaagatgttcctcgtcttgtaagttcacagcatgtagattctgtggagaaaatcatatcagttgtgttcaagtcacttccatcaaatttcaaccaattcatcagatgggtggctgagatccgaattacagaggactcaaaccaaaatctcagcgcagaggagaagtctaggctgaaactaaaggtatcttcaggataatttttcatagaaacctaaagctgcatggaaactgttctgttggtttatgtgacacatgattttaatcataactttaatctaccgca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M34572_sequence/1
type: Sequence
persistent identity: BBa M34572 sequence
display id: BBa_M34572_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aatttgcactctttacctctctttgactccgtgagattcgaggattgttagtttcttgtgatgtgtagtctttgaagcaggggatttttattgtattgaggaagaagatggggtttgagccgttggattggtattgcaagccggtgccgaatggtgtgtggactaaaactgtggattatgcgtttggtgcatacacgccttgtgctattgactcttttgtgcttggtatctctcatctggttctgttgattctgtgtctttatcgcttgtggctcatcacgaaggatcacaaagtggataagttctgcttgaggtctaaatggtttagctattttctggctcttttggctgcttatgctactgcggagcctttgtttagattggtcatgaggatctctgttttggatttggatggagctgggtttcctccctatgaggcgtttatgttggtccttgaggcttttgcttggggttctgctttggtcatgactgttgtggaaactaaaacgtatatccatgaactccgttggtatgtcagattcgctgtcatttatgctcttgtgggagacatggtgttgttaaatcttgttctctctgttaaggagtactatggcagttttaaactgtatctttacataagcgaggtggcagttcaggttgcatttggaaccctcttgtttgtgtatttccctaatttggacccttaccctggttacacaccagttgggactgaaaattccgaggattacgagtatgaagagcttcctggaggagaaaatatatgtcctgagaggcatgcaaatttatttgacagtatcttcttctcatggttgaacccattgatgactctgggatcaaaacgacctctcaccgagaaggatgtatggcatctggacacttgggataaaactgaaactcttatgaggagcttccagaagtcctgggataaggaactagaaaagcccaaaccgtggcttttgagagcactgaacaacagccttgggggaaggttttggtggggtggcttttggaagattgggaatgactgttcacagttcgtggggcctcttctactgaatgagctcttaaagtcaatgcaacttaatgaaccagcgtggataggttacatctatgcaatctcaatctttgttggagtggtattgggggttttatgtgaagctcagtatttccaaaatgtgatgcgtgttggttaccggcttaggtctgcactgattgctgctgtgttccgaaaatctttgaggctaactaatgaggggcggaagaagtttcaaacaggaaaaataacaaacttaatgactactgatgctgagtcgctgtagcaaatctgccaatcacttcataccatgtggtcggcgccatttcgtataattgtagcactggttctcctctatcaacaattgggtgttgcctcgatcattggtgcattgtttcttgtccttatgttccccatacagactgttattataagcaaaacgcagaagttaacaaaagaagggttgcagcgtactgacaagagaattggcctaatgaatgaggttttagcggcaatggatacagtgaagtgttacgcttgggaaaacagttttcagtccaaggttcaaactgtacgtgatgatgaattatcttggttccggaaagcacaactcctgtcagcgttcaatatgttcatactaaacagcattcctgtcctcgtgactgttgtttcatttggtgtgttctcattgcttggaggagatctgacacctgcaagagcgtttacgtcactctctctattttctgtgcttcgcttccctttattcatgcttccaaacattataactcagatggtaaatgctaatgtatccttaaaccgtttggaggaggtactgtcaaccgaagagagagttctcttaccgaatcctcccattgaacctggacagccagctatctcaataagaaatggatacttctcctgggattcaaaggcggataggccaaca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M34573_sequence/1
type: Sequence
persistent identity: BBa M34573 sequence
display id: BBa_M34573_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaatctccaggtaaagcttctccctttttctctgttttctctgttttgttattctcccttttctccattgtagctttttcctgtaaagtgggattgatagttttgtttcatggatttcaaatttgtgttatttgactcgataccatcttaaatgcagagtcttttcgtgataataaaattatggattcgtttcaaagttttttttttttcgtatggaaaacacttgagctctctcaatcttgtagtcttgactcttgatgattcttctatgttctcgttgtgattgcttgtcactgttctatcttttatatatgattaaatgcaattttgcccctttttacgcgcgaatgtatttattatctttcgcactctgggtccatttcttgtcacttgagcacataatgattgatttatgactttttaaagttatgaaaatttattatttttgttgctatggttttttggaattagaagctcatttcaaagttgttgattttctttgcagggtagggaattggtgtggtagcttgtgatgcactgtgtttgagggaaaggaaaggataacgatggggtttgagtttattgaatggtattgtaagccggtgcctaatggtgtttggacaaaacaagtggctaatgcatttggtgcatacacgccttgtgctactgactcttttgtgcttggtatctctcaactggttctgttggttctgtgcctgtatcgtatatggctcgccttaaaggatcacaaggtggagaggttctgtttgaggtcaagattgtataactatttcctggctttgttggctgcgtatgctactgctgagcctttgtttagattgatcatggggatttcagttttagattttgatggacctggacttcctccttttgaggcattcggattgggtgtcaaagcttttgcttggggcgctgtaatggtcatgattttaatggaaactaaaatttacatccgtgaactccgttggtatgtcaggtttgctgtcatatatgctcttgtgggggatatggtcttgttaaatcttgttctctcagtcaaggagtactatagcagttatgttctgtatctctacacaagcgaagtgggagctcaggttctgtttggaatcctcttgtttatgcatcttcccaatttggatacttaccctggctacatgccagtgcggagtgaaactgtggatgattatgagtatgaagagatttctgatggacaacaaatatgccctgagaagcatgcaaatatatttgacaaaatcttcttctcatggatgaatcccttgatgactttgggatctaaaaggcctctaacagagaaggatgtgtggtatctggacacttgggatcagactgaaactctgttcacgagtttccagcattcctgggataaggaactacaaaagccgcaaccgtggctgttgagagcattgaacaatagcctgggaggaaggttttggtggggaggattttggaagatcgggaatgattgctcacagtttgtgggacctcttttactgaatcaactcttaaagtcaatgcaagaggatgcgccagcttggatgggttacatctatgcgttctcaatctttgttggagtggtgttcggggtgctatgtgaagctcaatatttccagaatgtcatgcgtgttggttaccgactgagatctgctctgattgctgctgtgttccgcaaatcgttgaggttaactaatgaaggtcgtagaaagtttcaaacaggaaagataaccaacttaatgacgactgatgccgaatctcttcagcaaatatgccaatcacttcataccatgtggtcggctccatttcgtataattatagcactgattctcctctatcagcaattgggtgttgcctcgctcattggtgcattgttgttggtccttatgttccctttacagactgttattataagcaaaatgcagaagctgacaaaggaaggtttacagcgtactgacaagagaat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J52016_sequence/1
type: Sequence
persistent identity: BBa J52016 sequence
display id: BBa_J52016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagctcgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatatgca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J63003_sequence/1
type: Sequence
persistent identity: BBa J63003 sequence
display id: BBa_J63003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgccgccaccatggag
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z