BBa_M36009
1
BBa_M36009
5' Bicistronic UTR (strong), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
c/o Vivek Mutalik et al. BIOFAB Emeryville
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
please see long description.
false
Drew Endy
BBa_M36006
1
BBa_M36006
glucose1phosphate adenylyltransferase
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation.
Codes for the glucose-1-phosphate adenylyltransferase enzyme, utilized in the rate-limiting first step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10379 for full enzyme details.
false
false
_848_
0
9641
9
Not in stock
false
This mutation results in greater glycogen production compared to WT.
false
Jesse Palmer, Elliot Lui
annotation2119203
1
stop
range2119203
1
1294
1296
annotation2119202
1
C->T
range2119202
1
199
199
annotation2119064
1
start
range2119064
1
1
3
BBa_M36724
1
BBa_M36724
5' Bicistronic UTR (strong), does not include ATG start
2011-04-27T11:00:00Z
2015-05-08T01:14:06Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Vivek says, "This is (the) Makoff design, it gives good expression and is seq independent of the Schoner design."
false
Drew Endy
BBa_M36008
1
BBa_M36008
predicted glycogen synthesis protein
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Genomic sequence glgS as provided on EcoCyc database.
Codes for a protein involved in the biosynthesis of glycogen. Overproduction stimulates glycogen synthesis. Influences the first two enzymes in the e coli glycogen biosynthesis pathway (glucose-1-phosphate adenylyltransferase, glycogen synthase). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-REG-SUMMARY&object=EG11381 for more information.
false
false
_848_
0
9641
9
Not in stock
false
false
Jesse Palmer, Elliot Lui
annotation2119219
1
stop
range2119219
1
199
201
annotation2119218
1
start
range2119218
1
1
3
BBa_M36001
1
BBa_M36001
5' Bicistronic UTR (medium), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Please see long description.
false
Drew Endy
BBa_M36007
1
BBa_M36007
glycogen synthase
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Comes from standard genomic sequence (glgA) for the e coli glycogen synthase enzyme.
Codes for the glycogen synthase enzyme, utilized in the second step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://biocyc.org/ECOLI/new-image?type=GENE&object=EG10377 for full enzyme details.
false
false
_848_
0
9641
9
Not in stock
false
false
Jesse Palmer, Elliot Lui
annotation2119212
1
Start Codon
range2119212
1
1
3
annotation2119213
1
Stop Codon
range2119213
1
1432
1434
BBa_M36012
1
BBa_M36012
Glycogen Synthesis Actuator (ideal)
2011-04-27T11:00:00Z
2015-05-08T01:14:02Z
Expresses the glgC, glgA, and glgS proteins/enzymes usually not expressed in e. coli.
false
false
_848_
0
9642
9
Not in stock
false
Using different RBSs so that secondary structures and self pairing don't occur.
false
Elliott Lui and Jesse Palmer
component2118438
1
BBa_M36001
component2118437
1
BBa_M36006
component2118442
1
BBa_M36010
component2118440
1
BBa_M36724
component2118441
1
BBa_M36008
component2118439
1
BBa_M36007
component2118436
1
BBa_M36009
annotation2118440
1
BBa_M36724
range2118440
1
2825
2909
annotation2118438
1
BBa_M36001
range2118438
1
1344
1390
annotation2118437
1
BBa_M36006
range2118437
1
48
1343
annotation2118442
1
BBa_M36010
range2118442
1
3111
3192
annotation2118439
1
BBa_M36007
range2118439
1
1391
2824
annotation2118441
1
BBa_M36008
range2118441
1
2910
3110
annotation2118436
1
BBa_M36009
range2118436
1
1
47
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_M36001_sequence
1
tatagagggtattaataatgtatggattaaagggggaggcataacaa
BBa_M36008_sequence
1
atggatcatagtcttaattctttaaataatttcgatttcctggcgcgtagttttgccagaatgcacgcagaaggtcgcccggtcgatattctggccgttactggtaacatggatgaagaacatagaacctggttttgcgcacgttatgcctggtattgtcaacagatgatgcaggcaagagagctggagttagagcactga
BBa_M36007_sequence
1
atgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcgcaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcgcacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaattcaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcagctggcgctactcggcgcgggcgatccggtgctgcaggaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggtgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaatag
BBa_M36012_sequence
1
tatagagggtattaataatgtatggattaaaaggggaggtataacaaatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatctgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaattcgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccggcggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaattcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataatatagagggtattaataatgtatggattaaagggggaggcataacaaatgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcgcaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcgcacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaattcaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcagctggcgctactcggcgcgggcgatccggtgctgcaggaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggtgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctgatggatcatagtcttaattctttaaataatttcgatttcctggcgcgtagttttgccagaatgcacgcagaaggtcgcccggtcgatattctggccgttactggtaacatggatgaagaacatagaacctggttttgcgcacgttatgcctggtattgtcaacagatgatgcaggcaagagagctggagttagagcactgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36006_sequence
1
atggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatctgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaattcgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccggcggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaattcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataa
BBa_M36009_sequence
1
tatagagggtattaataatgtatggattaaaaggggaggtataacaa
BBa_M36724_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z