BBa_M36009 1 BBa_M36009 5' Bicistronic UTR (strong), does not include ATG start 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z c/o Vivek Mutalik et al. BIOFAB Emeryville 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information. false false _848_ 0 52 432 Not in stock false please see long description. false Drew Endy BBa_M36006 1 BBa_M36006 glucose1phosphate adenylyltransferase 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z The sequence provided here is from the CL1136 strain, with an Arg67Cys mutation. Codes for the glucose-1-phosphate adenylyltransferase enzyme, utilized in the rate-limiting first step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE&object=EG10379 for full enzyme details. false false _848_ 0 9641 9 Not in stock false This mutation results in greater glycogen production compared to WT. false Jesse Palmer, Elliot Lui annotation2119203 1 stop range2119203 1 1294 1296 annotation2119202 1 C->T range2119202 1 199 199 annotation2119064 1 start range2119064 1 1 3 BBa_M36724 1 BBa_M36724 5' Bicistronic UTR (strong), does not include ATG start 2011-04-27T11:00:00Z 2015-05-08T01:14:06Z Vivek Mutalik et al. c/o BIOFAB Emeryville. 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information. false false _848_ 0 52 432 Not in stock false Vivek says, "This is (the) Makoff design, it gives good expression and is seq independent of the Schoner design." false Drew Endy BBa_M36008 1 BBa_M36008 predicted glycogen synthesis protein 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Genomic sequence glgS as provided on EcoCyc database. Codes for a protein involved in the biosynthesis of glycogen. Overproduction stimulates glycogen synthesis. Influences the first two enzymes in the e coli glycogen biosynthesis pathway (glucose-1-phosphate adenylyltransferase, glycogen synthase). See http://BioCyc.org/ECOLI/NEW-IMAGE?type=GENE-IN-REG-SUMMARY&object=EG11381 for more information. false false _848_ 0 9641 9 Not in stock false false Jesse Palmer, Elliot Lui annotation2119219 1 stop range2119219 1 199 201 annotation2119218 1 start range2119218 1 1 3 BBa_M36001 1 BBa_M36001 5' Bicistronic UTR (medium), does not include ATG start 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Vivek Mutalik et al. c/o BIOFAB Emeryville. 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information. false false _848_ 0 52 432 Not in stock false Please see long description. false Drew Endy BBa_M36007 1 BBa_M36007 glycogen synthase 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Comes from standard genomic sequence (glgA) for the e coli glycogen synthase enzyme. Codes for the glycogen synthase enzyme, utilized in the second step of e coli glycogen biosynthesis (as described in EcoCyc database). See http://biocyc.org/ECOLI/new-image?type=GENE&object=EG10377 for full enzyme details. false false _848_ 0 9641 9 Not in stock false false Jesse Palmer, Elliot Lui annotation2119212 1 Start Codon range2119212 1 1 3 annotation2119213 1 Stop Codon range2119213 1 1432 1434 BBa_M36012 1 BBa_M36012 Glycogen Synthesis Actuator (ideal) 2011-04-27T11:00:00Z 2015-05-08T01:14:02Z Expresses the glgC, glgA, and glgS proteins/enzymes usually not expressed in e. coli. false false _848_ 0 9642 9 Not in stock false Using different RBSs so that secondary structures and self pairing don't occur. false Elliott Lui and Jesse Palmer component2118438 1 BBa_M36001 component2118437 1 BBa_M36006 component2118442 1 BBa_M36010 component2118440 1 BBa_M36724 component2118441 1 BBa_M36008 component2118439 1 BBa_M36007 component2118436 1 BBa_M36009 annotation2118440 1 BBa_M36724 range2118440 1 2825 2909 annotation2118438 1 BBa_M36001 range2118438 1 1344 1390 annotation2118437 1 BBa_M36006 range2118437 1 48 1343 annotation2118442 1 BBa_M36010 range2118442 1 3111 3192 annotation2118439 1 BBa_M36007 range2118439 1 1391 2824 annotation2118441 1 BBa_M36008 range2118441 1 2910 3110 annotation2118436 1 BBa_M36009 range2118436 1 1 47 BBa_M36010 1 BBa_M36010 Transcription Terminator (Strong) 2011-04-25T11:00:00Z 2015-05-08T01:14:02Z Guillaume Cambray et al. c/o BIOFAB Emeryville E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator. false true _848_ 0 52 432 Not in stock false please see long description false Drew Endy BBa_M36001_sequence 1 tatagagggtattaataatgtatggattaaagggggaggcataacaa BBa_M36008_sequence 1 atggatcatagtcttaattctttaaataatttcgatttcctggcgcgtagttttgccagaatgcacgcagaaggtcgcccggtcgatattctggccgttactggtaacatggatgaagaacatagaacctggttttgcgcacgttatgcctggtattgtcaacagatgatgcaggcaagagagctggagttagagcactga BBa_M36007_sequence 1 atgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcgcaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcgcacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaattcaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcagctggcgctactcggcgcgggcgatccggtgctgcaggaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggtgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaatag BBa_M36012_sequence 1 tatagagggtattaataatgtatggattaaaaggggaggtataacaaatggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatctgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaattcgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccggcggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaattcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataatatagagggtattaataatgtatggattaaagggggaggcataacaaatgcaggttttacatgtatgttcagagatgttcccgctgcttaaaaccggcggtctggctgatgttattggggcattacccgcagcacaaatcgcagacggcgttgacgctcgcgtactgttgcctgcatttcccgatattcgccgtggcgtgaccgatgcgcaggtagtatcccgtcgtgataccttcgccggacatatcacgctgttgttcggtcattacaacggggttggcatttacctgattgacgcgccgcatctctatgatcgtccgggaagcccgtatcacgataccaacttatttgcctataccgacaacgtattgcgttttgcgctgctggggtgggttggggcagaaatggccagcgggcttgacccattctggcgtcctgatgtggtgcatgcgcacgactggcatgcaggccttgcgcctgcgtatctggcggcgcgcgggcgtccggcgaagtcggtgtttactgtgcacaacctggcctatcaaggcatgttttatgcacatcacatgaatgacatccaattgccatggtcattctttaatattcatgggctggaattcaacggacaaatctctttcctgaaggccggtctgtactatgccgatcacattacggcggtcagtccaacctacgctcgcgagatcaccgaaccgcagtttgcctacggtatggaaggtctgttgcaacagcgtcaccgtgaagggcgtctttccggcgtactgaacggcgtggacgagaaaatctggagtccagagacggacttactgttggcctcgcgttacacccgcgatacgttggaagataaagcggaaaataagcgccagttacaaatcgcaatggggcttaaggttgacgataaagtgccgctttttgcagtggtgagccgtctgaccagccagaaaggtctcgacctggtgctggaagccttaccgggtcttctggagcagggcgggcagctggcgctactcggcgcgggcgatccggtgctgcaggaaggtttccttgcggcggcagcggaataccccggtcaggtgggcgttcagattggctatcacgaagcattttcgcatcgcattatgggcggcgcggacgtcattctggtgcccagccgttttgaaccgtgcggcttaacgcaactttatggattgaagtacggtacgctgccgttagtgcggcgcaccggtgggcttgctgatacggtttctgactgttctcttgagaaccttgcagatggcgtcgccagtgggtttgtctttgaagatagtaatgcctggtcgctgttacgggctattcgacgtgcttttgtactgtggtcccgtccttcactgtggcggtttgtgcaacgtcaggctatggcaatggattttagctggcaggtcgcggcgaagtcgtaccgtgagctttactatcgcttgaaataggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctgatggatcatagtcttaattctttaaataatttcgatttcctggcgcgtagttttgccagaatgcacgcagaaggtcgcccggtcgatattctggccgttactggtaacatggatgaagaacatagaacctggttttgcgcacgttatgcctggtattgtcaacagatgatgcaggcaagagagctggagttagagcactgatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36010_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36006_sequence 1 atggttagtttagagaagaacgatcacttaatgttggcgcgccagctgccattgaaatctgttgccctgatactggcgggaggacgtggtacccgcctgaaggatttaaccaataagcgagcaaaaccggccgtacacttcggcggtaagttccgcattatcgactttgcgctgtctaactgcatcaactccgggatctgtcgtatgggcgtgatcacccagtaccagtcccacactctggtgcagcacattcagcgcggctggtcattcttcaatgaagaaatgaacgagtttgtcgatctgctgccagcacagcagagaatgaaaggggaaaactggtatcgcggcaccgcagatgcggtcacccaaaacctcgacattatccgccgttataaagcggaatacgtggtgatcctggcgggcgaccatatctacaagcaagactactcgcgtatgcttatcgatcacgtcgaaaaaggcgcacgttgcaccgttgcttgtatgccagtaccgattgaagaagcctccgcatttggcgttatggcggttgatgagaacgataaaattatcgaattcgttgaaaaacctgctaacccgccgtcaatgccgaacgatccgagcaaatctctggcgagtatgggtatctacgtctttgacgccgactatctgtatgaactgctggaagaagacgatcgcgatgagaactccagccacgactttggcaaagatttgattcccaagatcaccgaagccggtctggcctatgcgcacccgttcccgctctcttgcgtacaatccgacccggatgccgagccgtactggcgcgatgtgggtacgctggaagcttactggaaagcgaacctcgatctggcctctgtggtgccggaactggatatgtacgatcgcaattggccaattcgcacctacaatgaatcattaccgccagcgaaattcgtgcaggatcgctccggtagccacgggatgacccttaactcactggtttccggcggttgtgtgatctccggttcggtggtggtgcagtccgttctgttctcgcgcgttcgcgtgaattcattctgcaacattgattccgccgtattgttaccggaagtatgggtaggtcgctcgtgccgtctgcgccgctgcgtcatcgatcgtgcttgtgttattccggaaggcatggtgattggtgaaaacgcagaggaagatgcacgtcgtttctatcgttcagaagaaggcatcgtgctggtaacgcgcgaaatgctacggaagttagggcataaacaggagcgataa BBa_M36009_sequence 1 tatagagggtattaataatgtatggattaaaaggggaggtataacaa BBa_M36724_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z