BBa_M36020 1 BBa_M36020 Transcription Factor lldR 2014-10-22T11:00:00Z 2015-05-08T01:14:02Z Sequence obtained from http://biocyc.org/ECOL386585/sequence?type=GENE&object=GJFA-4449 This is the segment of DNA that codes for the lldR protein, which is a transcription factor that regulates the lldPRD operon. In the absence of lactate, lldR binds to the operon lldPRD and inhibits transcription of the gene downstream. Includes coding sequence from START to STOP codon. Promotor, RBS, terminator, and lldPRD operon not included. false false _848_ 0 24147 9 Not in stock false - false Rachel Lim BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_J61127 1 BBa_J61127 Ribosome Binding Site Family Member 2007-04-24T11:00:00Z 2015-08-31T01:59:49Z N/A {{JCA_Arkin_RBSFamily}} false false _95_ 0 483 95 In stock false N/A true John Anderson BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M36019 1 BBa_M36019 Lactate Sensor 2014-10-22T11:00:00Z 2015-05-08T01:14:02Z lldR sequence obtained from http://biocyc.org/ECOL386585/sequence?type=GENE&object=GJFA-4449. The rest obtained from Parts Registry. This sequence contains a promoter, RBS, coding sequence for lldR transcription factor, and a operon/promoter sequence. In the absence of lactate, lldR protein binds to the operon and prevents transcription downstream. Lactate binds to lldR and causes it to release the operon, so transcription downstream can continue. false false _848_ 0 24147 9 Not in stock false - false Rachel Lim component2429120 1 BBa_M36021 component2429112 1 BBa_M36022 component2429119 1 BBa_B0015 component2429109 1 BBa_J23110 component2429111 1 BBa_M36020 component2429110 1 BBa_J61127 annotation2429110 1 BBa_J61127 range2429110 1 44 55 annotation2429120 1 BBa_M36021 range2429120 1 1001 1332 annotation2429111 1 BBa_M36020 range2429111 1 62 838 annotation2429119 1 BBa_B0015 range2429119 1 864 992 annotation2429112 1 BBa_M36022 range2429112 1 847 855 annotation2429109 1 BBa_J23110 range2429109 1 1 35 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_M36022 1 BBa_M36022 Pre-Terminator Spacer 2014-10-22T11:00:00Z 2015-05-08T01:14:02Z Randomly generated. Short spacer sequence which separates coding region from terminator. false false _848_ 0 24147 9 Not in stock false - false Rachel Lim BBa_M36021 1 BBa_M36021 lldPRD operon promoter 2014-10-22T11:00:00Z 2015-05-08T01:14:02Z Sourced from Parts Registry: BBa_K822000 - removed bases from RBS onwards This is the promoter region for the lldPRD operon (previously named lct) of E. coli. The promoter is repressed/activated by the regulatory protein lldR encoded by the operon. In the absence of L-lactate, lldR binds to two operator sites O1 and O2 in the promoter region and inhibits expression. (Description obtained from Parts' Registry BBa_K822000, by Jarle Pahr) false false _848_ 0 24147 9 Not in stock false - false Rachel Lim BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J61127_sequence 1 aaagagtggaac BBa_M36020_sequence 1 atgattgttttacccagacgcctgtcagacgaggttgccgatcgtgtgcgggcgctgattgatgaaaaaaacctggaagcgggcatgaagttgcccgctgagcgccaactggcgatgcaactcggcgtatcacgtaattcactacgcgaggcgctggcaaaactggtgagcgaaggcgtgctgctcagtcgacgcggcggcgggacgtttattcgctggcgtcatgacacatggtcggagcaaaacatcgtccagccgctaaaaacgctgatggccgatgatccggattacagtttcgatattctggaagcccgctacgccattgaagccagcaccgcatggcatgcggcaatgcgcgccacacctggcgacaaagaaaagattcagctttgctttgaagcaacgctaagtgaagacccggatatcgcctcacaagcggacgttcgttttcatctggcgattgccgaagcctcacataacatcgtgctgctgcaaaccatacgcggtttcttcgatgtcctgcaatcctcagtgaagcatagccgtcagcggatgtatctggtgccaccggttttttcacaactgaccgaacaacatcaggctgtcattgacgccatttttgctggtgatgccgacggggcgcgtaaagcaatgatggcgcaccttagttttgttcacaccaccatgaaacgattcgatgaagatcaggctcgccacgcacggattacccgcctgcccggtgagcataatgagcattcgagggagaaaaacgcatga BBa_M36022_sequence 1 acatccaac BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_M36021_sequence 1 cacattcctataggccgagtaaggtgttcacgccgcatccggcaagataaggcgctctggatcaacaacctaagggcaattctctgatgaggattgcccttttctttaccagacatctccccccacaagaattggccctaccaattcttcgcttatctgacctctggttcacaatttcccaattaaaactcacatcaatgttgccaatacataacatttagttaaccattcattgtcattatccctacacaacacaattggcagtgccacttttacacaacgtgtgacaaggagatgagcaacagactcattacacgatgtgcgtggactcc BBa_M36019_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagagtggaactactagatgattgttttacccagacgcctgtcagacgaggttgccgatcgtgtgcgggcgctgattgatgaaaaaaacctggaagcgggcatgaagttgcccgctgagcgccaactggcgatgcaactcggcgtatcacgtaattcactacgcgaggcgctggcaaaactggtgagcgaaggcgtgctgctcagtcgacgcggcggcgggacgtttattcgctggcgtcatgacacatggtcggagcaaaacatcgtccagccgctaaaaacgctgatggccgatgatccggattacagtttcgatattctggaagcccgctacgccattgaagccagcaccgcatggcatgcggcaatgcgcgccacacctggcgacaaagaaaagattcagctttgctttgaagcaacgctaagtgaagacccggatatcgcctcacaagcggacgttcgttttcatctggcgattgccgaagcctcacataacatcgtgctgctgcaaaccatacgcggtttcttcgatgtcctgcaatcctcagtgaagcatagccgtcagcggatgtatctggtgccaccggttttttcacaactgaccgaacaacatcaggctgtcattgacgccatttttgctggtgatgccgacggggcgcgtaaagcaatgatggcgcaccttagttttgttcacaccaccatgaaacgattcgatgaagatcaggctcgccacgcacggattacccgcctgcccggtgagcataatgagcattcgagggagaaaaacgcatgatactagagacatccaactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcacattcctataggccgagtaaggtgttcacgccgcatccggcaagataaggcgctctggatcaacaacctaagggcaattctctgatgaggattgcccttttctttaccagacatctccccccacaagaattggccctaccaattcttcgcttatctgacctctggttcacaatttcccaattaaaactcacatcaatgttgccaatacataacatttagttaaccattcattgtcattatccctacacaacacaattggcagtgccacttttacacaacgtgtgacaaggagatgagcaacagactcattacacgatgtgcgtggactcc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z