BBa_M36028
1
BBa_M36028
phiX174 gene D - mutated rbs and start codon of E, and rbs of J
2011-05-02T11:00:00Z
2015-05-08T01:14:02Z
wt phiX174, then mutated by Jon Rodriguez and Adrian Sierra
Took the wt gene K from coliphage phiX174, and then mutated it synonymously in order to destroy the RBS and start codon of the overlapping gene E, and the overlapping RBS of gene J.
false
false
_848_
0
9553
9
Not in stock
false
Made only synonymous changes, and tried to keep the codon usage bias as similar as possible to wt, according to this table http://openwetware.org/wiki/Escherichia_coli/Codon_usage
false
Jon Rodriguez
annotation2119080
1
stop codon
range2119080
1
457
459
annotation2119081
1
CDS
range2119081
1
1
459
annotation2119093
1
g->a
range2119093
1
168
168
annotation2119100
1
a->t
range2119100
1
450
450
annotation2119090
1
t->c
range2119090
1
180
180
annotation2119096
1
a->g
range2119096
1
447
447
annotation2119079
1
start codon
range2119079
1
1
3
BBa_M36028_sequence
1
atgagtcaagttactgaacaatccgtacgtttccagaccgctttggcctctattaagctcattcaggcttctgccgttttggatttaaccgaagatgatttcgattttctgacgagtaacaaagtttggattgctactgaccgctctcgtgctcgtcgctgcgttgaagcttgcgtttacggtacgctggactttgtgggataccctcgctttcctgctcctgttgagtttattgctgccgtcattgcttattatgttcatcccgtcaacattcaaacggcctgtctcatcatggaaggcgctgaatttacggaaaacattattaatggcgtcgagcgtccggttaaagccgctgaattgttcgcgtttaccttgcgtgtacgcgcaggaaacactgacgttcttactgacgcagaagaaaacgtgcgtcaaaaattacgtgcggagggtgtgatgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z