BBa_M36034 1 BBa_M36034 BCD02 - Bicistronic Design 02 2013-10-23T11:00:00Z 2015-05-08T01:14:02Z We found it in a parts registry linked to from the paper detailed in the description of this part This part is the bicistronic with the highest expression of GFP in a paper or bicistronics entitled "Precise and reliable gene expression via standard transcription and translation initiation elements" (Mutalek 2012). false false _848_ 0 19059 9 Not in stock false The start codon at the end had to be removed false Jacob Waggoenr BBa_M36032 1 BBa_M36032 Composite part for Bone Sialoprotein Actuator 2013-10-23T11:00:00Z 2015-05-08T01:14:02Z It comes from a database (unknown biological origin) and human DNA. This is a part composed of BCD02, a bicistronic, the bone sialoprotein gene, and the M13_central_T_linker_rrnD_T1 terminator. The terminator and bicistronic are both very high efficiency. false false _848_ 0 19059 9 Not in stock false We had to remove an extra start codon from the bicistronic. false Jacob Waggoenr component2370556 1 BBa_M36033 component2370555 1 BBa_M36036 component2370554 1 BBa_M36034 annotation2370556 1 BBa_M36033 range2370556 1 1051 1245 annotation2370555 1 BBa_M36036 range2370555 1 92 1042 annotation2370554 1 BBa_M36034 range2370554 1 1 85 BBa_M36033 1 BBa_M36033 M13_central_T_linker_rrnD_T1 Terminator 2013-10-23T11:00:00Z 2015-05-08T01:14:02Z We found it in a parts registry linked to BioFab This is a double terminator that terminates with ~99.8% efficiency. It is 195 bp long. false false _848_ 0 19059 9 Not in stock false None in particular false Jacob Waggoenr BBa_M36036 1 BBa_M36036 Human Bone Sialoprotein Gene 2013-10-23T11:00:00Z 2015-05-08T01:14:02Z Biologically, it originates from humans. This gene encodes for human bone sialoprotein, a protein that has been shown to catalyze the nucleation of hydroxyapatite crystals. false false _848_ 0 19059 9 Not in stock false The original DNA contained a large number of palindromes that we had to perform silent mutations on in order to bring down production costs. false Jacob Waggoenr BBa_M36034_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttcta BBa_M36032_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctatactagatgaaaacagccctgatcctgctgtccattcttggcatggcatgtgccttcagcatgaagaatttgcatcgccgcgttaaaatcgaagatagcgaagaaaacggtgttttcaagtaccgcccccgctattacttgtataaacacgcctatttctatccgcatctgaagcgctttcctgtccagggaagctcggattcttccgaagagaatggcgacgacagctcggaagaagaggaggaagaagaggaaacttctaacgaaggtgaaaataacgaggaatccaacgaggatgaagactctgaagcggaaaatacgacactcagcgccactaccctgggctatggcgaggatgccacccccggcaccgggtataccggactcgccgcgatccagttaccaaaaaaggcaggagatattacgaataaagctaccaaagaaaaagaaagtgatgaggaggaagaagaggaggaagaggggaatgaaaacgaggagagtgaggcggaagtagacgagaacgaacagggtatcaacggaacgtccactaactctaccgaagctgaaaacggtaatggctctagcggtggcgacaatggtgaagagggcgaagaggagagcgtgaccggcgctaacgccgaagatactaccgaaacgggtcgtcagggcaaaggtacgtctaaaacgactacctctccgaacggcggatttgagccaacaactccgccacaagtatatcgtaccacatcaccaccgtttggcaaaaccaccacagtggaatatgaaggcgagtatgagtatacgggtgccaatgaatatgacaacggatatgagatctatgaaagcgagaacggcgagccacgtggtgataactatcgcgcatatgaggatgaatattcttactttaagggccaaggttatgacggctatgatggtcaaaattactaccatcaccaatactagagaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacatgaaaaaattattatttgatgatcagatagcggcggggaactgccagacatcaaataaaacaaaaggctcagtcggaagactgggccttttgttttatctgttgtttgtcggtgaacactctcccg BBa_M36033_sequence 1 aaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacatgaaaaaattattatttgatgatcagatagcggcggggaactgccagacatcaaataaaacaaaaggctcagtcggaagactgggccttttgttttatctgttgtttgtcggtgaacactctcccg BBa_M36036_sequence 1 atgaaaacagccctgatcctgctgtccattcttggcatggcatgtgccttcagcatgaagaatttgcatcgccgcgttaaaatcgaagatagcgaagaaaacggtgttttcaagtaccgcccccgctattacttgtataaacacgcctatttctatccgcatctgaagcgctttcctgtccagggaagctcggattcttccgaagagaatggcgacgacagctcggaagaagaggaggaagaagaggaaacttctaacgaaggtgaaaataacgaggaatccaacgaggatgaagactctgaagcggaaaatacgacactcagcgccactaccctgggctatggcgaggatgccacccccggcaccgggtataccggactcgccgcgatccagttaccaaaaaaggcaggagatattacgaataaagctaccaaagaaaaagaaagtgatgaggaggaagaagaggaggaagaggggaatgaaaacgaggagagtgaggcggaagtagacgagaacgaacagggtatcaacggaacgtccactaactctaccgaagctgaaaacggtaatggctctagcggtggcgacaatggtgaagagggcgaagaggagagcgtgaccggcgctaacgccgaagatactaccgaaacgggtcgtcagggcaaaggtacgtctaaaacgactacctctccgaacggcggatttgagccaacaactccgccacaagtatatcgtaccacatcaccaccgtttggcaaaaccaccacagtggaatatgaaggcgagtatgagtatacgggtgccaatgaatatgacaacggatatgagatctatgaaagcgagaacggcgagccacgtggtgataactatcgcgcatatgaggatgaatattcttactttaagggccaaggttatgacggctatgatggtcaaaattactaccatcaccaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z