BBa_M36050
1
BBa_M36050
LexA gene
2011-05-01T11:00:00Z
2015-05-08T01:14:02Z
escherichia coli k-12
LexA is a repressor that represses SOS response genes coding for DNA polymerases required for repairing DNA damage.
false
false
_848_
0
9234
9
Not in stock
false
N/A
false
Ryan Kent, Evan Clark
annotation2119199
1
start
range2119199
1
1
1
annotation2119200
1
stop
range2119200
1
609
609
annotation2118549
1
lexA
range2118549
1
1
609
BBa_M36051
1
BBa_M36051
LexA regulated promoter
2011-05-01T11:00:00Z
2015-05-08T01:14:02Z
escherichia coli k -12
This is the promoter sequence for the LexA gene. The lexA protein regulates its own promoter. There is a common binding site for the lexA protein found in this promoter that is also found in ~40 other SOS genes.
false
true
_848_
0
9234
9
Not in stock
false
We know exactly were the lexA protein binds, but we are not entirely sure of the exact deliniation of the promoter itself. Therefore this sequence may contain superfluous information.
false
Ryan Kent
annotation2119366
1
lexA binding
range2119366
1
65
68
annotation2119367
1
8 nt spacer
range2119367
1
69
76
annotation2119368
1
lexA binding
range2119368
1
77
80
BBa_M36001
1
BBa_M36001
5' Bicistronic UTR (medium), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Please see long description.
false
Drew Endy
BBa_J23106
1
BBa_J23106
constitutive promoter family member
2006-08-13T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_M36053
1
BBa_M36053
DNA damage sensor
2011-05-01T11:00:00Z
2015-05-08T01:14:02Z
composite
This part senses damaged DNA by using endogenous recA to cleave the provided lexA and prevent it from repressing the provided lexA promoter.
false
false
_848_
0
9234
9
Not in stock
false
This construct is using a medium strength promoter and 5'UTR so that the amount of lexA won't be so great as to not allow for endogenous levels of recA
false
Ryan Kent
component2118570
1
BBa_J23106
component2118574
1
BBa_M36051
component2118572
1
BBa_M36050
component2118571
1
BBa_M36001
component2118573
1
BBa_M36010
annotation2118572
1
BBa_M36050
range2118572
1
83
691
annotation2118570
1
BBa_J23106
range2118570
1
1
35
annotation2118573
1
BBa_M36010
range2118573
1
692
773
annotation2118571
1
BBa_M36001
range2118571
1
36
82
annotation2118574
1
BBa_M36051
range2118574
1
774
868
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_M36001_sequence
1
tatagagggtattaataatgtatggattaaagggggaggcataacaa
BBa_M36051_sequence
1
cccttccagaattcgataaatctctggtttattgtgcagtttatggttccaaaatcgccttttgctgtatatactcacagcataactgtatatac
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_J23106_sequence
1
tttacggctagctcagtcctaggtatagtgctagc
BBa_M36050_sequence
1
atgaaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccacttctggcgcaacagcatattgaaggtcattatcaggtcgatccttccttattcaagccgaatgctgatttcctgctgcgcgtcagcgggatgtcgatgaaagatatcggcattatggatggtgacttgctggcagtgcataaaactcaggatgtacgtaacggtcaggtcgttgtcgcacgtattgatgacgaagttaccgttaagcgcctgaaaaaacagggcaataaagtcgaactgttgccagaaaatagcgagtttaaaccaattgtcgttgaccttcgtcagcagagcttcaccattgaagggctggcggttggggttattcgcaacggcgactggctgtaa
BBa_M36053_sequence
1
tttacggctagctcagtcctaggtatagtgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatgaaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccacttctggcgcaacagcatattgaaggtcattatcaggtcgatccttccttattcaagccgaatgctgatttcctgctgcgcgtcagcgggatgtcgatgaaagatatcggcattatggatggtgacttgctggcagtgcataaaactcaggatgtacgtaacggtcaggtcgttgtcgcacgtattgatgacgaagttaccgttaagcgcctgaaaaaacagggcaataaagtcgaactgttgccagaaaatagcgagtttaaaccaattgtcgttgaccttcgtcagcagagcttcaccattgaagggctggcggttggggttattcgcaacggcgactggctgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccccttccagaattcgataaatctctggtttattgtgcagtttatggttccaaaatcgccttttgctgtatatactcacagcataactgtatatac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z