BBa_M36054
type: ComponentDefinition
persistent identity: BBa M36054
display id: BBa_M36054
version: 1
was generated by: iGEM to SBOL conversion
description: DNA damage sensor with gemini actuator (medium LexA generator)
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: composite
owned by: james
owned by: myers
mutable description: DNA damage sensor with a PoPs output attached to a gemini actuator. The sensor uses a medium strength promoter and a medium strength 5' UTR
discontinued: false
dominant: false
experience: experience/None
group u list: _833_848_
m user id: 0
owner id: 9234
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This uses a medium strength promoter and 5'UTR
star: false
creator: Ryan Kent
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_M36709
component: BBa_M36053
sequence annotation: BBa_M36053
sequence annotation: BBa_M36709
sequence: https://synbiohub.org/public/igem/BBa_M36054_sequence/1

BBa_M36709
type: Component
persistent identity: component2118860
display id: component2118860
version: 1
top level: BBa_M36054
definition: BBa_M36709
access: public

BBa_M36053
type: Component
persistent identity: component2118850
display id: component2118850
version: 1
top level: BBa_M36054
definition: BBa_M36053
access: public

BBa_M36053
type: SequenceAnnotation
persistent identity: annotation2118850
display id: annotation2118850
version: 1
top level: BBa_M36054
location: https://synbiohub.org/public/igem/BBa_M36054/annotation2118850/range2118850/1
component: BBa_M36053

https://synbiohub.org/public/igem/BBa_M36054/annotation2118850/range2118850/1
type: Range
persistent identity: range2118850
display id: range2118850
version: 1
top level: BBa_M36054
direction: direction/reverse
start: 1
end: 868
orientation: inline

BBa_M36709
type: SequenceAnnotation
persistent identity: annotation2118860
display id: annotation2118860
version: 1
top level: BBa_M36054
location: https://synbiohub.org/public/igem/BBa_M36054/annotation2118860/range2118860/1
component: BBa_M36709

https://synbiohub.org/public/igem/BBa_M36054/annotation2118860/range2118860/1
type: Range
persistent identity: range2118860
display id: range2118860
version: 1
top level: BBa_M36054
direction: direction/reverse
start: 869
end: 1915
orientation: inline

BBa_M36053
type: ComponentDefinition
persistent identity: BBa M36053
display id: BBa_M36053
version: 1
was generated by: iGEM to SBOL conversion
description: DNA damage sensor
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: composite
owned by: james
owned by: myers
mutable description: This part senses damaged DNA by using endogenous recA to cleave the provided lexA and prevent it from repressing the provided lexA promoter.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 9234
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This construct is using a medium strength promoter and 5'UTR so that the amount of lexA won't be so great as to not allow for endogenous levels of recA
star: false
creator: Ryan Kent
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_M36051
component: BBa_M36010
component: BBa_M36050
component: BBa_M36001
component: BBa_J23106
sequence annotation: BBa_J23106
sequence annotation: BBa_M36001
sequence annotation: BBa_M36050
sequence annotation: BBa_M36010
sequence annotation: BBa_M36051
sequence: https://synbiohub.org/public/igem/BBa_M36053_sequence/1

BBa_M36051
type: Component
persistent identity: component2118574
display id: component2118574
version: 1
top level: BBa_M36053
definition: BBa_M36051
access: public

BBa_M36010
type: Component
persistent identity: component2118573
display id: component2118573
version: 1
top level: BBa_M36053
definition: BBa_M36010
access: public

BBa_M36050
type: Component
persistent identity: component2118572
display id: component2118572
version: 1
top level: BBa_M36053
definition: BBa_M36050
access: public

BBa_M36001
type: Component
persistent identity: component2118571
display id: component2118571
version: 1
top level: BBa_M36053
definition: BBa_M36001
access: public

BBa_J23106
type: Component
persistent identity: component2118570
display id: component2118570
version: 1
top level: BBa_M36053
definition: BBa_J23106
access: public

BBa_J23106
type: SequenceAnnotation
persistent identity: annotation2118570
display id: annotation2118570
version: 1
top level: BBa_M36053
location: https://synbiohub.org/public/igem/BBa_M36053/annotation2118570/range2118570/1
component: BBa_J23106

https://synbiohub.org/public/igem/BBa_M36053/annotation2118570/range2118570/1
type: Range
persistent identity: range2118570
display id: range2118570
version: 1
top level: BBa_M36053
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_M36001
type: SequenceAnnotation
persistent identity: annotation2118571
display id: annotation2118571
version: 1
top level: BBa_M36053
location: https://synbiohub.org/public/igem/BBa_M36053/annotation2118571/range2118571/1
component: BBa_M36001

https://synbiohub.org/public/igem/BBa_M36053/annotation2118571/range2118571/1
type: Range
persistent identity: range2118571
display id: range2118571
version: 1
top level: BBa_M36053
direction: direction/reverse
start: 36
end: 82
orientation: inline

BBa_M36050
type: SequenceAnnotation
persistent identity: annotation2118572
display id: annotation2118572
version: 1
top level: BBa_M36053
location: https://synbiohub.org/public/igem/BBa_M36053/annotation2118572/range2118572/1
component: BBa_M36050

https://synbiohub.org/public/igem/BBa_M36053/annotation2118572/range2118572/1
type: Range
persistent identity: range2118572
display id: range2118572
version: 1
top level: BBa_M36053
direction: direction/reverse
start: 83
end: 691
orientation: inline

BBa_M36010
type: SequenceAnnotation
persistent identity: annotation2118573
display id: annotation2118573
version: 1
top level: BBa_M36053
location: https://synbiohub.org/public/igem/BBa_M36053/annotation2118573/range2118573/1
component: BBa_M36010

https://synbiohub.org/public/igem/BBa_M36053/annotation2118573/range2118573/1
type: Range
persistent identity: range2118573
display id: range2118573
version: 1
top level: BBa_M36053
direction: direction/reverse
start: 692
end: 773
orientation: inline

BBa_M36051
type: SequenceAnnotation
persistent identity: annotation2118574
display id: annotation2118574
version: 1
top level: BBa_M36053
location: https://synbiohub.org/public/igem/BBa_M36053/annotation2118574/range2118574/1
component: BBa_M36051

https://synbiohub.org/public/igem/BBa_M36053/annotation2118574/range2118574/1
type: Range
persistent identity: range2118574
display id: range2118574
version: 1
top level: BBa_M36053
direction: direction/reverse
start: 774
end: 868
orientation: inline

BBa_M36709
type: ComponentDefinition
persistent identity: BBa M36709
display id: BBa_M36709
version: 1
was generated by: iGEM to SBOL conversion
description: Gemini Protein Generator
created: 2011-05-02T11:00:00Z
modified: 2015-05-08T01:14:06Z
mutable provenance: Gemini, a Bifunctional Enzymatic and Fluorescent Reporter of Gene Expression. PLOS One. Lance Martin et al.
owned by: james
owned by: myers
mutable description: A medium strength RBS expressing Gemini, ending with a strong terminator.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9654
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Test Actuator
star: false
creator: Jeff Quinn and Wyatt Woodson
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_E0051
component: BBa_B0032
sequence annotation: BBa_B0032
sequence annotation: BBa_E0051
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_M36709_sequence/1

BBa_B0010
type: Component
persistent identity: component2118635
display id: component2118635
version: 1
top level: BBa_M36709
definition: BBa_B0010
access: public

BBa_E0051
type: Component
persistent identity: component2118634
display id: component2118634
version: 1
top level: BBa_M36709
definition: BBa_E0051
access: public

BBa_B0032
type: Component
persistent identity: component2118629
display id: component2118629
version: 1
top level: BBa_M36709
definition: BBa_B0032
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2118629
display id: annotation2118629
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_M36709/annotation2118629/range2118629/1
type: Range
persistent identity: range2118629
display id: range2118629
version: 1
top level: BBa_M36709
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0051
type: SequenceAnnotation
persistent identity: annotation2118634
display id: annotation2118634
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
component: BBa_E0051

https://synbiohub.org/public/igem/BBa_M36709/annotation2118634/range2118634/1
type: Range
persistent identity: range2118634
display id: range2118634
version: 1
top level: BBa_M36709
direction: direction/reverse
start: 14
end: 967
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2118635
display id: annotation2118635
version: 1
top level: BBa_M36709
location: https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M36709/annotation2118635/range2118635/1
type: Range
persistent identity: range2118635
display id: range2118635
version: 1
top level: BBa_M36709
direction: direction/forward
start: 968
end: 1047
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_J23106
type: ComponentDefinition
persistent identity: BBa J23106
display id: BBa_J23106
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-13T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23106_sequence/1

BBa_M36001
type: ComponentDefinition
persistent identity: BBa M36001
display id: BBa_M36001
version: 1
was generated by: iGEM to SBOL conversion
description: 5' Bicistronic UTR (medium), does not include ATG start
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Vivek Mutalik et al. c/o BIOFAB Emeryville.
owned by: james
owned by: myers
mutable description: 5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
discontinued: false
dominant: false
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Please see long description.
star: false
creator: Drew Endy
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M36001_sequence/1

BBa_M36010
type: ComponentDefinition
persistent identity: BBa M36010
display id: BBa_M36010
version: 1
was generated by: iGEM to SBOL conversion
description: Transcription Terminator (Strong)
created: 2011-04-25T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: Guillaume Cambray et al. c/o BIOFAB Emeryville
owned by: james
owned by: myers
mutable description: E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
discontinued: false
dominant: true
group u list: _848_
m user id: 0
owner id: 52
owning group id: 432
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: please see long description
star: false
creator: Drew Endy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence: https://synbiohub.org/public/igem/BBa_M36010_sequence/1

BBa_M36050
type: ComponentDefinition
persistent identity: BBa M36050
display id: BBa_M36050
version: 1
was generated by: iGEM to SBOL conversion
description: LexA gene
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: escherichia coli k-12
owned by: james
owned by: myers
mutable description: LexA is a repressor that represses SOS response genes coding for DNA polymerases required for repairing DNA damage.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 9234
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: Ryan Kent, Evan Clark
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: lexA
sequence annotation: start
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_M36050_sequence/1

lexA
type: SequenceAnnotation
persistent identity: annotation2118549
display id: annotation2118549
version: 1
top level: BBa_M36050
location: https://synbiohub.org/public/igem/BBa_M36050/annotation2118549/range2118549/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M36050/annotation2118549/range2118549/1
type: Range
persistent identity: range2118549
display id: range2118549
version: 1
top level: BBa_M36050
direction: direction/forward
start: 1
end: 609
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2119199
display id: annotation2119199
version: 1
top level: BBa_M36050
location: https://synbiohub.org/public/igem/BBa_M36050/annotation2119199/range2119199/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M36050/annotation2119199/range2119199/1
type: Range
persistent identity: range2119199
display id: range2119199
version: 1
top level: BBa_M36050
direction: direction/forward
start: 1
end: 1
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2119200
display id: annotation2119200
version: 1
top level: BBa_M36050
location: https://synbiohub.org/public/igem/BBa_M36050/annotation2119200/range2119200/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M36050/annotation2119200/range2119200/1
type: Range
persistent identity: range2119200
display id: range2119200
version: 1
top level: BBa_M36050
direction: direction/forward
start: 609
end: 609
orientation: inline

BBa_M36051
type: ComponentDefinition
persistent identity: BBa M36051
display id: BBa_M36051
version: 1
was generated by: iGEM to SBOL conversion
description: LexA regulated promoter
created: 2011-05-01T11:00:00Z
modified: 2015-05-08T01:14:02Z
mutable provenance: escherichia coli k -12
owned by: james
owned by: myers
mutable description: This is the promoter sequence for the LexA gene. The lexA protein regulates its own promoter. There is a common binding site for the lexA protein found in this promoter that is also found in ~40 other SOS genes.
discontinued: false
dominant: true
group u list: _848_
m user id: 0
owner id: 9234
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We know exactly were the lexA protein binds, but we are not entirely sure of the exact deliniation of the promoter itself. Therefore this sequence may contain superfluous information.
star: false
creator: Ryan Kent
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: lexA binding
sequence annotation: 8 nt spacer
sequence: https://synbiohub.org/public/igem/BBa_M36051_sequence/1

lexA binding
type: SequenceAnnotation
persistent identity: annotation2119366
display id: annotation2119366
version: 1
top level: BBa_M36051
location: https://synbiohub.org/public/igem/BBa_M36051/annotation2119366/range2119366/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36051/annotation2119366/range2119366/1
type: Range
persistent identity: range2119366
display id: range2119366
version: 1
top level: BBa_M36051
direction: direction/forward
start: 65
end: 68
orientation: inline

8 nt spacer
type: SequenceAnnotation
persistent identity: annotation2119367
display id: annotation2119367
version: 1
top level: BBa_M36051
location: https://synbiohub.org/public/igem/BBa_M36051/annotation2119367/range2119367/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M36051/annotation2119367/range2119367/1
type: Range
persistent identity: range2119367
display id: range2119367
version: 1
top level: BBa_M36051
direction: direction/forward
start: 69
end: 76
orientation: inline

lexA binding
type: SequenceAnnotation
persistent identity: annotation2119368
display id: annotation2119368
version: 1
top level: BBa_M36051
location: https://synbiohub.org/public/igem/BBa_M36051/annotation2119368/range2119368/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M36051/annotation2119368/range2119368/1
type: Range
persistent identity: range2119368
display id: range2119368
version: 1
top level: BBa_M36051
direction: direction/forward
start: 77
end: 80
orientation: inline

BBa_E0051
type: ComponentDefinition
persistent identity: BBa E0051
display id: BBa_E0051
version: 1
was generated by: iGEM to SBOL conversion
description: lacZa.GFP fusion
created: 2009-03-01T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: amino acids 3-60 from lacZ and amino acids 2-end of GFP (E0043)
owned by: james
owned by: myers
mutable description: This part is a fusion protein between GFP and the alpha fragment of lacZ. It can be measured via fluorescence from GFP or through an assay for beta-galactosidase in a strain containing the omega fragment of lacZ.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 22
owning group id: 84
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The amino acid linker AGGSEGGGSEHHHHHHGSE is between the lacZa and GFP. The 6-his can also facilitate detection on a Western blot, for purification, etc.
star: false
creator: Austin Che
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: lacZa
sequence annotation: linker
sequence annotation: GFP
sequence: https://synbiohub.org/public/igem/BBa_E0051_sequence/1

lacZa
type: SequenceAnnotation
persistent identity: annotation2001726
display id: annotation2001726
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001726/range2001726/1
type: Range
persistent identity: range2001726
display id: range2001726
version: 1
top level: BBa_E0051
direction: direction/forward
start: 1
end: 180
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation2001727
display id: annotation2001727
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0051/annotation2001727/range2001727/1
type: Range
persistent identity: range2001727
display id: range2001727
version: 1
top level: BBa_E0051
direction: direction/forward
start: 181
end: 237
orientation: inline

GFP
type: SequenceAnnotation
persistent identity: annotation2001728
display id: annotation2001728
version: 1
top level: BBa_E0051
location: https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0051/annotation2001728/range2001728/1
type: Range
persistent identity: range2001728
display id: range2001728
version: 1
top level: BBa_E0051
direction: direction/forward
start: 238
end: 954
orientation: inline

https://synbiohub.org/public/igem/BBa_M36054_sequence/1
type: Sequence
persistent identity: BBa M36054 sequence
display id: BBa_M36054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatgaaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccacttctggcgcaacagcatattgaaggtcattatcaggtcgatccttccttattcaagccgaatgctgatttcctgctgcgcgtcagcgggatgtcgatgaaagatatcggcattatggatggtgacttgctggcagtgcataaaactcaggatgtacgtaacggtcaggtcgttgtcgcacgtattgatgacgaagttaccgttaagcgcctgaaaaaacagggcaataaagtcgaactgttgccagaaaatagcgagtttaaaccaattgtcgttgaccttcgtcagcagagcttcaccattgaagggctggcggttggggttattcgcaacggcgactggctgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccccttccagaattcgataaatctctggtttattgtgcagtttatggttccaaaatcgccttttgctgtatatactcacagcataactgtatatactcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36053_sequence/1
type: Sequence
persistent identity: BBa M36053 sequence
display id: BBa_M36053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagctatagagggtattaataatgtatggattaaagggggaggcataacaaatgaaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccacttctggcgcaacagcatattgaaggtcattatcaggtcgatccttccttattcaagccgaatgctgatttcctgctgcgcgtcagcgggatgtcgatgaaagatatcggcattatggatggtgacttgctggcagtgcataaaactcaggatgtacgtaacggtcaggtcgttgtcgcacgtattgatgacgaagttaccgttaagcgcctgaaaaaacagggcaataaagtcgaactgttgccagaaaatagcgagtttaaaccaattgtcgttgaccttcgtcagcagagcttcaccattgaagggctggcggttggggttattcgcaacggcgactggctgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccccttccagaattcgataaatctctggtttattgtgcagtttatggttccaaaatcgccttttgctgtatatactcacagcataactgtatatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36709_sequence/1
type: Sequence
persistent identity: BBa M36709 sequence
display id: BBa_M36709_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23106_sequence/1
type: Sequence
persistent identity: BBa J23106 sequence
display id: BBa_J23106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtatagtgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36001_sequence/1
type: Sequence
persistent identity: BBa M36001 sequence
display id: BBa_M36001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatagagggtattaataatgtatggattaaagggggaggcataacaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36010_sequence/1
type: Sequence
persistent identity: BBa M36010 sequence
display id: BBa_M36010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36050_sequence/1
type: Sequence
persistent identity: BBa M36050 sequence
display id: BBa_M36050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaagcgttaacggccaggcaacaagaggtgtttgatctcatccgtgatcacatcagccagacaggtatgccgccgacgcgtgcggaaatcgcgcagcgtttggggttccgttccccaaacgcggctgaagaacatctgaaggcgctggcacgcaaaggcgttattgaaattgtttccggcgcatcacgcgggattcgtctgttgcaggaagaggaagaagggttgccgctggtaggtcgtgtggctgccggtgaaccacttctggcgcaacagcatattgaaggtcattatcaggtcgatccttccttattcaagccgaatgctgatttcctgctgcgcgtcagcgggatgtcgatgaaagatatcggcattatggatggtgacttgctggcagtgcataaaactcaggatgtacgtaacggtcaggtcgttgtcgcacgtattgatgacgaagttaccgttaagcgcctgaaaaaacagggcaataaagtcgaactgttgccagaaaatagcgagtttaaaccaattgtcgttgaccttcgtcagcagagcttcaccattgaagggctggcggttggggttattcgcaacggcgactggctgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36051_sequence/1
type: Sequence
persistent identity: BBa M36051 sequence
display id: BBa_M36051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttccagaattcgataaatctctggtttattgtgcagtttatggttccaaaatcgccttttgctgtatatactcacagcataactgtatatac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0051_sequence/1
type: Sequence
persistent identity: BBa E0051 sequence
display id: BBa_E0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgcgggcggcagcgaaggcggcggcagcgaacatcatcatcatcatcatggcagcgaacgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactaccctgacctatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z