BBa_M36060 1 BBa_M36060 Poly-beta-hydroxybutyrate Growth Actuator 2015-10-22T11:00:00Z 2015-10-23T12:59:48Z Source of the part is the genomic sequence Xanthomonas campestris pv. raphani 756C, specifically G0CFI5. Part codes for the mRNA sequence for poly-beta-hydroxybutyrate polymerase, which synthesizes poly-3-hydroxybutyrate from glucose. Part should be inserted within pD431-SR with T7 promoter. Poly-3-hydroxybutyrate is a biodegradable polymer, which will break down after it fulfills its intended function and this part is intended to provide a means with which to synthesize the described useful polymer. Part is intended to be transformed into E. coli and grown on kanamycin plates. Expression of the vector can be induced with IPTG. true false _848_ 29108 29108 9 false Design considerations include keeping the DNA sequence less than 1500 bp, avoiding the sites BsbI, BsaI, and BsmBI, and having less than 5 repeats of 10 bp. false Taylor Marie Chavez BBa_M36060_sequence 1 atgaagggtcctcttggtttttctgccgaagatttgatgcaagagacgttgtccatgcagcgtaaactgcgtgaaggcctgaaactcctgcctggtgtggaggatgttgactatggtgttactgaacgccaggaagtttggcgcgacggcaaagtcgtcttgtatcgtttcgtcggagacgcggcaccagttgcacgtacaccgttgctgattgtgtatgctttagttaatcgtccttacatggtagatctgcaggccgaccgttccctcgttaaaggtctgttgggccatggccaggatgtgtatgtgctggattggggttatccggatcgttccgaacgttatctgacgctggaagattatttacttcgctacatcgatggcgccgttgaccatcttcgcgctgcaagtggtctggaggcggttgatgttctgggtatctgccaaggtgggacgtttgctctctgctacgcagcattagaacgcgctaaaatccgcaatctgatcaccatggtgaccccagtcgacttccacaccccggacaatatgttatcgaactgggcccgcatggtggacgttgatctgtttgtggataccatggggaatgtaccggcggatttaatgaacgcgagctatctcatgctgaaaccgttccgcctgaacctccaaaagtatgtgggattactggatattctcgatgacaagcaggccttagaagacttcctgcgtatggaaaaatggatctttgatagccccgacctcgcgggcgaagcattccgtgagttcgtgacccagttttatcagcgtaatggcctggtgaccggcgatgtccgtattggcggtcaagctgtggatctgcacgcagtggatatgccggtcctgaacatctatgcagaacatgatcatctggttccgccggacgcttctcgcgcgctgcgtggacttgtcgggtcggaagattataccgaactgtcatttcgtggagggcacattggtatttatgtctcggggcgcgcccagcgcgaagtgcctgtagctattcatcgttggttacaggcccgcggtggtaacggttga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z