BBa_M36070
1
BBa_M36070
Bicistronic RBS - BIOFAB ID: apFAB681 (BCD1)
2012-12-07T12:00:00Z
2015-05-08T01:14:02Z
Biofab.org's BCD+GOI promoter library: http://io.biofab.org:5000/simple/bd_gois
Bicistronic RBS with high levels of expression. Strain performance: 435.43 Strain performance SD: 53.55
false
false
_848_
0
15056
9
Not in stock
false
N/a
false
Alex Rosay, Justin Diep, Ben Blankenmeister
BBa_M36071
1
SoDA
K12 e.coli native Manganese Superoxide Dismutase
2012-12-07T12:00:00Z
2015-05-08T01:14:02Z
This sequence is derived from the amino acid sequence of Mn-SOD protein found from Durfee et. al at the University of Wisconsin which also provided the entire genomic sequence of the E. coli (strain: k-12, substrain: DH10B) genome.
Coding sequence for the enzyme Mn-SOD. This protein catalyzes the dismutation of superoxide, a free radical. Sequence found by converting the amino acid sequence of Mn-SOD protein native to Escherichia coli, the organism for which it is also optimized for.
false
false
_848_
0
15057
9
Not in stock
false
This DNA sequence is converted from the amino acid sequence of Mn-SOD protein using the online converter from In-Silico.
false
Alex Rosay, Justin Diep, Ben Blankenmeister
annotation2213706
1
Start Codon
range2213706
1
1
3
BBa_M36072
1
SoDA
Superoxide Dismutase A Actuator
2012-12-07T12:00:00Z
2015-05-08T01:14:02Z
Durfee et al. "The Complete Genome Sequence of Escherichia coli DH10B: Insights into the Biology of a Laboratory Workhorse" 1 Feb 2008.
This device includes a strong bicistronic RBS (BBa_M36070), the enzyme sequence of sodA (BBa_M36071), and a strong terminator (BBa_B0010) that takes the input signal PoPs to produce sodA, an enzyme that dismutates superoxide free radicals.
false
false
_848_
0
15057
9
Not in stock
false
The encoded enzyme sequence (BBa_M36070) was derived from converting the amino acid sequence of sodA obtained from Durfee et al. from the University of Wisconsin (obtained from E. coli strain: k-12, substrain: DH10B genome).
false
Justin Diep, Alex Rosay, Ben Blankenmeister
component2213707
1
BBa_M36070
component2213709
1
BBa_M36071
component2213710
1
BBa_B0010
annotation2213710
1
BBa_B0010
range2213710
1
721
800
annotation2213709
1
BBa_M36071
range2213709
1
95
712
annotation2213707
1
BBa_M36070
range2213707
1
1
88
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M36070_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctaatg
BBa_M36071_sequence
1
atgtcttatactctgccaagtctgccctacgcgtatgacgctctcgagccccattttgataagcagacgatggaaattcatcacacaaaacatcatcaaacctacgtcaataatgccaatgctgctctcgagtccttgcccgagttcgccaacttgccagtggaggagctgataaccaaattggaccagctaccggccgataaaaagaccgtactgcggaacaacgcgggcgggcatgctaaccattccttgttctggaaaggactgaagaagggcacgacgctgcagggtgatctgaaggcagccatagaacgggatttcgggtccgttgataactttaaagcggaattcgaaaaggcagcagcaagccgatttgggtccggatgggcttggcttgtattgaagggcgacaaattggcggtagtctcgacggcaaaccaagatagcccactaatgggtgaggcgatctcaggggcgtcaggcttcccgattatgggcctggacgtgtgggaacacgcgtactacctgaagttccagaaccgccggcccgactacattaaagagttttggaatgtagttaattgggatgaagctgcagcccggttcgcggcaaagaaa
BBa_M36072_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctaatgtactagatgtcttatactctgccaagtctgccctacgcgtatgacgctctcgagccccattttgataagcagacgatggaaattcatcacacaaaacatcatcaaacctacgtcaataatgccaatgctgctctcgagtccttgcccgagttcgccaacttgccagtggaggagctgataaccaaattggaccagctaccggccgataaaaagaccgtactgcggaacaacgcgggcgggcatgctaaccattccttgttctggaaaggactgaagaagggcacgacgctgcagggtgatctgaaggcagccatagaacgggatttcgggtccgttgataactttaaagcggaattcgaaaaggcagcagcaagccgatttgggtccggatgggcttggcttgtattgaagggcgacaaattggcggtagtctcgacggcaaaccaagatagcccactaatgggtgaggcgatctcaggggcgtcaggcttcccgattatgggcctggacgtgtgggaacacgcgtactacctgaagttccagaaccgccggcccgactacattaaagagttttggaatgtagttaattgggatgaagctgcagcccggttcgcggcaaagaaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z