BBa_K416003
1
BBa_K416003
Yeast Secretion Tag
2010-06-22T11:00:00Z
2015-05-08T01:12:27Z
This part was sent to use from Dr. Sheldon Park of the University of Buffalo.
This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS.
false
false
_527_
0
7430
9
It's complicated
true
There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence.
false
John Phair
BBa_K392003
1
BBa_K392003
yeast ADH1 terminator
2010-10-09T11:00:00Z
2015-05-08T01:12:20Z
Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak).
Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream.
false
false
_528_
0
6220
9
It's complicated
true
Assembled by 3A method.
false
Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda
BBa_M36081
1
BBa_M36081
Tenebrio Molitor Antifreeze Protein Actuator With Secretion Tag
2014-04-29T11:00:00Z
2015-05-08T01:14:02Z
Gene of Interest from Uniprot, Kozac sequence, Terminator and Yeast secretion tag from parts registry
This is an actuator that will generate antifreeze proteins in yeast and hopefully, reduce ice growth within the yeast themselves. This part is specific because it has a yeast secretion tag on it because we do not know whether the signal peptide on the sequence will naturally be secreted (it is still an area of study within the field).
false
false
_848_
0
22183
9
Not in stock
false
We had to include the yeast secretion tag to make sure the antifreeze protein would go into the solution and exit the yeast cell.
false
Ashley Hammerbacher Celina Malav??
component2372811
1
BBa_M36084
component2372810
1
BBa_K416003
component2372812
1
BBa_K392003
component2372809
1
BBa_M36083
component2372808
1
BBa_K792001
annotation2372810
1
BBa_K416003
range2372810
1
370
483
annotation2372808
1
BBa_K792001
range2372808
1
1
21
annotation2372811
1
BBa_M36084
range2372811
1
492
512
annotation2372812
1
BBa_K392003
range2372812
1
521
649
annotation2372809
1
BBa_M36083
range2372809
1
28
363
BBa_K792001
1
MFa1-Kozak
Kozak sequence from yeast α-factor mating pheromone (MFα1)
2012-09-23T11:00:00Z
2015-05-08T01:13:23Z
http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=MFA1
The Kozak sequence is the eukaryotic analog of the bacterial RBS, it is short sequence that includes the ATG, required for proficient initiation of translation. It is known that the 5???UTR sequence of the MFα1 gene of yeast produces efficient initiation of translation.
This part is that specific region of the MFα1 gene.
false
false
_1047_
0
11811
9
Not in stock
false
None
false
Manuel Gim??nez
annotation2197872
1
start
range2197872
1
19
21
annotation2197870
1
BamHI restriction site
range2197870
1
1
6
annotation2197871
1
rbs
range2197871
1
7
21
BBa_M36083
1
BBa_M36083
Tenebrio Molitor Antifreeze Protein
2014-04-29T11:00:00Z
2015-05-08T01:14:02Z
Uniprot
The Antifreeze Protein DNA
false
false
_848_
0
22183
9
Not in stock
false
We simply got the sequence off of Uniprot so that we could create the basic part to integrate into our actuator
false
Ashley Hammerbacher Celina Malav??
BBa_M36084
1
BBa_M36084
Histidine Tag and Stop Codon
2014-04-29T11:00:00Z
2015-05-08T01:14:02Z
We know that histidine tags are a sequence of 6 Histidines and 1 stop codon
Histidine Tag and Stop Codon Sequences
false
false
_848_
0
22183
9
Not in stock
false
Histidine tag must be before stop codon
false
Ashley Hammerbacher Celina Malav??
BBa_M36083_sequence
1
atggcgtttaaaacctgcggctttagcaaaaaatggctggtgattgcggtgattgtgatgtgcctgtgcaccgaatgctattgccagtgcaccggcggcgcggattgcaccagctgcaccggcgcgtgcaccggctgcggcaactgcccgaacgcggtgacctgcaccaacagccagcattgcgtgaaagcgaacacctgcaccggcagcaccgattgcaacaccgcgcagacctgcaccaacagcaaagattgctttgaagcgaacacctgcaccgatagcaccaactgctataaagcgaccgcgtgcaccaacagcagcggctgcccgggccat
BBa_M36081_sequence
1
ggatccacgattaaaagaatgtactagatggcgtttaaaacctgcggctttagcaaaaaatggctggtgattgcggtgattgtgatgtgcctgtgcaccgaatgctattgccagtgcaccggcggcgcggattgcaccagctgcaccggcgcgtgcaccggctgcggcaactgcccgaacgcggtgacctgcaccaacagccagcattgcgtgaaagcgaacacctgcaccggcagcaccgattgcaacaccgcgcagacctgcaccaacagcaaagattgctttgaagcgaacacctgcaccgatagcaccaactgctataaagcgaccgcgtgcaccaacagcagcggctgcccgggccattactagatgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaagatactagagcatcatcatcatcatcattagtactagaggcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag
BBa_M36084_sequence
1
catcatcatcatcatcattag
BBa_K416003_sequence
1
atgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaaga
BBa_K392003_sequence
1
gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag
BBa_K792001_sequence
1
ggatccacgattaaaagaatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z