LOCUS BBa_M36092 6047 bp DNA linear 22-Dec-2024 DEFINITION S. cerevisiae actuator test rig ACCESSION BBa_M36092 VERSION BBa_M36092.1 FEATURES Location/Qualifiers promoter 1..128 /label=Pamp CDS 129..938 /label=KanR misc_feature 939..1742 /label=pUCori promoter 1757..2171 /label=Gal1 promoter misc_feature 2173..2883 /label=student actuators go here promoter 2899..3398 /label=Pleu CDS 3399..4493 /label=Leu polyA_site 4494..4593 /label=Leu polyA misc_feature 4598..6043 /label=2um ori ORIGIN 1 tcagaattgg ttaattggtt gtaacactga cccctatttg tttatttttc taaatacatt 61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 121 ggaagaatat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg 181 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa 241 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta 301 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc 361 cacttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg 421 cgatccccgg aaaaacagcg ttccaggtat tagaagaata tcctgattca ggtgaaaata 481 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcactc gattcctgtt tgtaattgtc 541 cttttaacag cgatcgcgta tttcgcctcg ctcaggcgca atcacgaatg aataacggtt 601 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga 661 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct 721 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag 781 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt 841 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata 901 aattgcaatt tcatttgatg ctcgatgagt ttttctaact catgaccaaa atcccttaac 961 gtgagttacg cgcgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 1021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 1081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 1141 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt tagcccacca 1201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 1261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 1321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 1381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 1441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 1501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 1561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 1621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 1681 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 1741 tcggggtcgt gcaggtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaac 1801 actctcctcc gtgcgtcctc ctcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg 1861 cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg 1921 aaaaattggc agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca 1981 taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg 2041 atgatttttg atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa 2101 tactttcaac attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa 2161 aaaattgtta aaatgacagc tttaactgaa ggggccaagc ttttcgaaaa agagatccca 2221 tatatcaccg aacttgaagg tgacgttgag ggtatgaagt ttatcataaa aggagaaggc 2281 acaggtgatg cgacaacggg tacaatcaag gcaaagtaca tttgtacaac tggggacctg 2341 cctgtcccat gggccacttt ggtgtctact ttgtcttacg gcgtacaatg ttttgctaag 2401 tacccttcac acatcaaaga tttctttaag tctgcaatgc ctgaaggata cacacaggaa 2461 cgtacaattt catttgaggg cgacggtgtc tataaaacaa gagctatggt tacttatgaa 2521 agaggttcca tctacaacag agtgacacta acgggcgaaa atttcaaaaa ggatggacat 2581 attttgcgta aaaacgtagc tttccaatgc ccaccatcaa tactatacat tctgccagat 2641 actgtaaaca atggtattag agtcgagttt aatcaagctt atgatataga aggtgtcact 2701 gaaaaattgg ttacaaaatg cagccaaatg aatagaccat tggcaggatc tgccgctgtg 2761 catatcccta gataccatca cattacctac cacaccaaat taagtaaaga cagggatgaa 2821 cgaagagatc atatgtgttt agttgaggtt gttaaggcag ttgatctcga cacttaccaa 2881 taaaacctgc acgcaaaata ggataattat actctatttc tcaacaagta attggttgtt 2941 tggccgagcg gtctaaggcg cctgattcaa gaaatatctt gaccgcagtt aactgtggga 3001 atactcaggt atcgtaagat gcaagagttc gaatctctta gcaaccatta tttttttcct 3061 caacataacg agaacacaca ggggcgctat cgcacagaat caaattcgat gactggaaat 3121 tttttgttaa tttcagaggt cgcctgacgc atataccttt ttcaactgaa aaattgggag 3181 aaaaaggaaa ggtgagagcg ccggaaccgg cttttcatat agaatagaga agcgttcatg 3241 actaaatgct tgcatcacaa tacttgaagt tgacaatatt atttaaggac ctattgtttt 3301 ttccaatagg tggttagcaa tcgtcttact ttctaacttt tcttaccttt tacatttcag 3361 caatatatat atatatattt caaggatata ccattctaat gtctgcccct aagaagatcg 3421 tcgttttgcc aggtgaccac gttggtcaag aaatcacagc cgaagccatt aaggttctta 3481 aagctatttc tgatgttcgt tccaatgtca agttcgattt cgaaaatcat ttaattggtg 3541 gtgctgctat cgatgctaca ggtgttccac ttccagatga ggcgctggaa gcctccaaga 3601 aggctgatgc cgttttgtta ggtgctgtgg gtggtcctaa atggggtacc ggtagtgtta 3661 gacctgaaca aggtttacta aaaatccgta aagaacttca attgtacgcc aacttaagac 3721 catgtaactt tgcatccgac tctcttttag acttatctcc aatcaagcca caatttgcta 3781 aaggtactga cttcgttgtt gtcagagaat tagtgggagg tatttacttt ggtaagagaa 3841 aggaagatga tggtgatggt gtcgcttggg atagtgaaca atacaccgtt ccagaagtgc 3901 aaagaatcac aagaatggcc gctttcatgg ccctacaaca tgagccacca ttgcctattt 3961 ggtccttgga taaagctaat gttttggcct cttcaagatt atggagaaaa actgtggagg 4021 aaaccatcaa gaacgaattc cctacattga aggttcaaca tcaattgatt gattctgccg 4081 ccatgatcct agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt 4141 ttggtgatat catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat 4201 ctgcgtcctt ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc 4261 acggttctgc tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg 4321 ctgcaatgat gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg 4381 cagttaaaaa ggttttggat gcaggcatca gaactggtga tttaggtggt tccaacagta 4441 ccaccgaagt cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt 4501 ctcttttttt atgatatttg tacataaact ttataaatga aattcataat agaaacgaca 4561 cgaaattaca aaatggaata tgttcatagg gtaacgctat gatccaatat caaaggaaat 4621 gatagcattg aaggatgaga ctaatccaat tgaggagtgg cagcatatag aacagctaaa 4681 gggtagtgct gaaggaagca tacgataccc cgcatggaat gggataatat cacaggaggt 4741 actagactac ctttcatcct acataaatag acgcatataa gtacgcattt aagcataaac 4801 acgcactatg ccgttcttct catgtatata tatatacagg caacacgcag atataggtgc 4861 gacgtgaaca gtgagctgta tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt 4921 cggaaacgct ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc 4981 agagcgcttt tgaaaaccaa aagcgctctg aagtcgcact ttcaaaaaac caaaaacgca 5041 ccggactgta acgagctact aaaatattgc gaataccgct tccacaaaca ttgctcaaaa 5101 gtatctcttt gctatatatc tctgtgctat atccctatat aacctaccca tccacctttc 5161 gctccttgaa cttgcatcta aactcgacct ctacattttt tatgtttatc tctagtatta 5221 ctctttagac aaaaaaattg tagtaagaac tattcataga gtgaatcgaa aacaatacga 5281 aaatgtaaac atttcctata cgtagtatat agagacaaaa tagaagaaac cgttcataat 5341 tttctgacca atgaagaatc atcaacgcta tcactttctg ttcacaaagt atgcgcaatc 5401 cacatcggta tagaatataa tcggggatgc ctttatcttg aaaaaatgca cccgcagctt 5461 cgctagtaat cagtaaacgc gggaagtgga gtcaggcttt ttttatggaa gagaaaatag 5521 acaccaaagt agccttcttc taaccttaac ggacctacag tgcaaaaagt tatcaagaga 5581 ctgcattata gagcgcacaa aggagaaaaa aagtaatcta agatgctttg ttagaaaaat 5641 agcgctctcg ggatgcattt ttgtagaaca aaaaagaagt atagattctt tgttggtaaa 5701 atagcgctct cgcgttgcat ttctgttctg taaaaatgca gctcagattc tttgtttgaa 5761 aaattagcgc tctcgcgttg catttttgtt ttacaaaaat gaagcacaga ttcttcgttg 5821 gtaaaatagc gctttcgcgt tgcatttctg ttctgtaaaa atgcagctca gattctttgt 5881 ttgaaaaatt agcgctctcg cgttgcattt ttgttctaca aaatgaagca cagatgcttc 5941 gttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 6001 tacattcaaa tatgtatccg ctcatgagac aataaccctg atattgg //