LOCUS BBa_M36094 9657 bp DNA linear 16-Jun-2024 DEFINITION S. elongatus cyanobacteria actuator test rig ACCESSION BBa_M36094 VERSION BBa_M36094.1 FEATURES Location/Qualifiers misc_feature 1..861 /label=E. coli ORI misc_feature 1072..2555 /label=NS2 site #1 CDS 2786..3868 /label=lacI repressor promoter 4100..4129 /label=Lac promoter misc_feature 4130..4171 /label=student actuators go here stem_loop 4172..4740 /label=rrnB transcription terminator CDS 4751..6002 /label=KanR cassette misc_feature 6008..6740 /label=NS2 site #2 ORIGIN 1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 301 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 601 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 1021 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttgtca 1081 tctgccggat gaggcaaaac cctgcctacg gcgcgattac atcgtcccag cgcgatcgct 1141 cttactgttg atggctcgtg cttaaaaaca atgcaaactt caccgtttca gctggtgatt 1201 ttcgactgtg atggtgtgct tgttgatagc ggaacgcatc actaatcgcg tctttgcaga 1261 catgctcaat gaactgggtc tgttggtgac tttggatgac atgtttgagc agtttgtggg 1321 tcattccatg gctgactgtc tcaaactaat tgagcgacgg ttaggcaatc ctccaccccc 1381 tgactttgtt cagcactatc aacgccgtac ccgtatcgcg ttagaaacgc atctacaagc 1441 cgttcctggg gttgaagagg ctttggatgc tcttgaattg ccctactgtg ttgcgtccag 1501 tggtgatcat caaaagatgc gaaccacact gagcctgacg aagctctggc cacgatttga 1561 gggacgaatc ttcagcgtga ctgaagtacc tcgcggcaag ccatttcccg atgtcttttt 1621 gttggccgcc gatcgcttcg gggttaatcc tacggcctgc gctgtgatcg aagacacccc 1681 cttgggagta gcggcaggcg tggcggcagg aatgcaagtg tttggctacg cgggttccat 1741 gcccgcttgg cgtctgcaag aagccggtgc ccatctcatt tttgacgata tgcgactgct 1801 gcccagtctg ctccaatcgt cgccaaaaga taactccaca gcattgccca atccctaacc 1861 cctgctcgcg ccgcaactac acactaaacc gttcctgcgc gatcgctctt actgttgatg 1921 gctcgtgctt aaaaacaatg caaccctaac cgtttcagct ggtgattttc ggacgatttg 1981 gcttacaggg ataactgaga gtcaacagcc tctgtccgtc attgcacacc catccatgca 2041 ctggggactt gactcatgct gaatcacatt tcccttgtcc attgggcgag aggggagggg 2101 aatcttctgg actcttcact aagcggcgat cgcaggttct tctacccaag cagtggcgat 2161 cgcttgattg cagtcttcaa tgctggcctc tgcagccatc gccgccacca aagcatcgta 2221 ggcgggacgt tgttgctcca gtaaagtctt cgcccgtaac aatccccagc gactgcgtaa 2281 atccgcttcg gcaggattgc gatcgagttg ccgccacagt tgtttccact gggcgcgatc 2341 gtcagctccc ccttccacgt tgccgtagac cagttgctct gccgctgcac cggccatcaa 2401 cacctgacac cactgttcca gcgatcgctg actgagttgc ccctgtgcgg cttcggcttc 2461 tagcgcagct gcttggaact gcacaccccc gcgaccaggt tgtccttggc gcagcgcttc 2521 ccacgctgag agggtgtagc ccgtcacggg taaccgatat ctgtacaccg cggggtacca 2581 ctagttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt 2641 catcaccgaa acgcgcgagg cagcagatca attcgcgcgc gaaggcgaag cggcatgcat 2701 ttacgttgac accatcgaat ggtgcaaaac ctttcgcggt atggcatgat agcgcccgga 2761 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ttatacgatg tcgcagagta 2821 tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc acgtttctgc 2881 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt 2941 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc 3001 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc 3061 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca 3121 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga 3181 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga 3241 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga 3301 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt 3361 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc 3421 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat 3481 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg 3541 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg 3601 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga 3661 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc 3721 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc 3781 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 3841 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagcgcgaat 3901 tgatctggtt tgacagctta tcatcgactg cacggtgcac caatgcttct ggcgtcaggc 3961 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc 4021 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca 4081 aatattctga aatgagctgt tgacaattaa tcatccggct cgtataatgt tttttatata 4141 tatatataga tgatggatga tagtagagta gagcttggcc ggatccggcc ggatccggga 4201 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg 4261 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa 4321 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa 4381 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac 4441 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg 4501 catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttattgagcc 4561 gttaccccac ctactagcta atcccatctg ggcacatccg atggcaagag gcccgaaggt 4621 ccccctcttt ggtcttgcga cgttatgcgg tattagctac cgtttccagt agttatcccc 4681 ctccatcagg cagtttccca gacattactc acccgtccgc cactcgtcag caaagaagca 4741 agcttagatc gacctgcagg gggggggggg aaagccacgt tgtgtctcaa aatctctgat 4801 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 4861 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgaggccgc 4921 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 4981 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc 5041 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 5101 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 5161 catggttact caccactgcg atccccggga aaacagcatt ccaggtatta gaagaatatc 5221 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 5281 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 5341 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 5401 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg 5461 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 5521 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 5581 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 5641 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag 5701 aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcggct 5761 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa 5821 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa 5881 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcctg 5941 gtatgagtca gcaacacctt cttcacgagg cagacctcag cgcccccccc cccctgcagg 6001 tcgatctggt aaccccagcg cggttgctac caagtagtga cccgcttcgt gatgcaaaat 6061 ccgctgacga tattcgggcg atcgctgctg aatgccatcg agcagtaacg tggcaccccg 6121 cccctgccaa gtcaccgcat ccagactgaa cagcaccaag aggctaaaac ccaatcccgc 6181 cggtagcagc ggagaactac ccagcattgg tcccaccaaa gctaatgccg tcgtggtaaa 6241 aatcgcgatc gccgtcagac tcaagcccag ttcgctcatg cttcctcatc taggtcacag 6301 tcttcggcga tcgcatcgat ctgatgctgc agcaagcgtt ttccataccg gcgatcgcgc 6361 cgtcgccctt tcgctgccgt ggcccgctta cgagctcgtt tatcgaccac gatcgcatcc 6421 aaatccgcga tcgcttccca gtccggcaat tcagtctggg gcgtccgttt cattaatcct 6481 gatcaggcac gaaattgctg tgcgtagtat cgcgcatagc ggccagcctc tgccaacagc 6541 gcatcgtgat tgcctgcctc aacaatctgg ccgcgctcca tcaccaagat gcggctggca 6601 ttacgaaccg tagccagacg gtgagcaatg ataaagaccg tccgtccctg catcacccgt 6661 tctagggcct cttgcaccaa ggtttcggac tcggaatcaa gcgccgaagt cgcctcatcc 6721 agaattaaaa tgcgtggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca 6781 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc 6841 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg 6901 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc 6961 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgatcga 7021 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact 7081 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca 7141 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg 7201 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc 7261 accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga cgcgctgggc 7321 tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat gattcttctc 7381 gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca ggtagatgac 7441 gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac ttcgatcact 7501 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca 7561 tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg 7621 agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga ttcaccactc 7681 caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc 7741 agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc tcgggcagcg 7801 ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct 7861 ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg 7921 actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg 7981 tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca 8041 tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc 8101 attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct 8161 cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct 8221 ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag 8281 tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa 8341 cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc 8401 ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg 8461 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 8521 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 8581 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 8641 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 8701 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 8761 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 8821 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 8881 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 8941 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 9001 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 9061 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 9121 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 9181 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 9241 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 9301 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 9361 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9421 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9481 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9541 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 9601 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacag //