LOCUS BBa_M36095 9052 bp DNA linear 25-Nov-2024 DEFINITION S. elongatus cyanobacteria sensor test rig ACCESSION BBa_M36095 VERSION BBa_M36095.1 FEATURES Location/Qualifiers misc_feature 1..1484 /label=NS2 site #1 misc_feature 1715..1751 /label=student sensors go here RBS 1752..1766 /label=RBS CDS 1767..2482 /label=cyanocitrus YFP stem_loop 2496..3064 /label=rrnB transcription terminator CDS 3075..4326 /label=KanR cassette misc_feature 4332..5064 /label=NS2 site #2 misc_feature 7982..8842 /label=E. coli ORI ORIGIN 1 agcttgtcat ctgccggatg aggcaaaacc ctgcctacgg cgcgattaca tcgtcccagc 61 gcgatcgctc ttactgttga tggctcgtgc ttaaaaacaa tgcaaacttc accgtttcag 121 ctggtgattt tcgactgtga tggtgtgctt gttgatagcg gaacgcatca ctaatcgcgt 181 ctttgcagac atgctcaatg aactgggtct gttggtgact ttggatgaca tgtttgagca 241 gtttgtgggt cattccatgg ctgactgtct caaactaatt gagcgacggt taggcaatcc 301 tccaccccct gactttgttc agcactatca acgccgtacc cgtatcgcgt tagaaacgca 361 tctacaagcc gttcctgggg ttgaagaggc tttggatgct cttgaattgc cctactgtgt 421 tgcgtccagt ggtgatcatc aaaagatgcg aaccacactg agcctgacga agctctggcc 481 acgatttgag ggacgaatct tcagcgtgac tgaagtacct cgcggcaagc catttcccga 541 tgtctttttg ttggccgccg atcgcttcgg ggttaatcct acggcctgcg ctgtgatcga 601 agacaccccc ttgggagtag cggcaggcgt ggcggcagga atgcaagtgt ttggctacgc 661 gggttccatg cccgcttggc gtctgcaaga agccggtgcc catctcattt ttgacgatat 721 gcgactgctg cccagtctgc tccaatcgtc gccaaaagat aactccacag cattgcccaa 781 tccctaaccc ctgctcgcgc cgcaactaca cactaaaccg ttcctgcgcg atcgctctta 841 ctgttgatgg ctcgtgctta aaaacaatgc aaccctaacc gtttcagctg gtgattttcg 901 gacgatttgg cttacaggga taactgagag tcaacagcct ctgtccgtca ttgcacaccc 961 atccatgcac tggggacttg actcatgctg aatcacattt cccttgtcca ttgggcgaga 1021 ggggagggga atcttctgga ctcttcacta agcggcgatc gcaggttctt ctacccaagc 1081 agtggcgatc gcttgattgc agtcttcaat gctggcctct gcagccatcg ccgccaccaa 1141 agcatcgtag gcgggacgtt gttgctccag taaagtcttc gcccgtaaca atccccagcg 1201 actgcgtaaa tccgcttcgg caggattgcg atcgagttgc cgccacagtt gtttccactg 1261 ggcgcgatcg tcagctcccc cttccacgtt gccgtagacc agttgctctg ccgctgcacc 1321 ggccatcaac acctgacacc actgttccag cgatcgctga ctgagttgcc cctgtgcggc 1381 ttcggcttct agcgcagctg cttggaactg cacacccccg cgaccaggtt gtccttggcg 1441 cagcgcttcc cacgctgaga gggtgtagcc cgtcacgggt aaccgatatc tgtacaccgc 1501 ggggtaccac tagttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 1561 tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg aaggcgaagc 1621 ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata 1681 gcgcccggaa gagagtcaat tcagggtggt gaattttttt agatgagggt gtagtatgta 1741 tgatgatgta taaggaggaa aaaaaatgtc taagggtgaa gagttgttta caggagttgt 1801 cccaatcttg gtggaactgg atggcgatgt caatggccat aagtttagtg ttagcggtga 1861 aggcgaaggt gatgcaacct acggcaagct gaccctcaaa ctgatctgca ccaccggtaa 1921 gctgcccgtt ccctggccca ccctggttac gaccctcggc tatggattga tgtgctttgc 1981 ccgttaccct gatcatatga agcagcatga tttttttaaa agtgctatgc ctgagggcta 2041 cgtgcaagag cggacgatct tcttcaaaga tgatggcaac tataaaacgc gcgccgaagt 2101 taaatttgaa ggggatacac tggtcaatcg catcgaactc aaggggattg attttaagga 2161 agatgggaac attctgggcc ataaattgga gtacaattac aattctcaca acgtttatat 2221 caccgcagat aaacaaaaga atggtattaa agcgaacttc aaaattcgtc ataatattga 2281 ggatggtggc gtgcaactgg ccgatcatta tcagcagaac acgcccattg gggacggccc 2341 tgtgttgctg ccggacaatc attacttgtc ctatcaatcg aaactgtcga aagacccaaa 2401 tgaaaaacgc gatcacatgg ttctcttgga atttgtcacg gctgcgggca ttacgcatgg 2461 tatggatgag ctgtataaat aaggccgcgc tagcaagctt ggccggatcc ggccggatcc 2521 gggagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg 2581 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt 2641 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat 2701 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc 2761 ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc acttctgagt 2821 tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttattg 2881 agccgttacc ccacctacta gctaatccca tctgggcaca tccgatggca agaggcccga 2941 aggtccccct ctttggtctt gcgacgttat gcggtattag ctaccgtttc cagtagttat 3001 ccccctccat caggcagttt cccagacatt actcacccgt ccgccactcg tcagcaaaga 3061 agcaagctta gatcgacctg cagggggggg ggggaaagcc acgttgtgtc tcaaaatctc 3121 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac 3181 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg 3241 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat 3301 gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg 3361 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta 3421 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat 3481 gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa 3541 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat 3601 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg 3661 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc 3721 tggcctgttg aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca 3781 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata 3841 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta 3901 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt 3961 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa 4021 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc 4081 ggctttgttg aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg 4141 acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca 4201 acaaagctct catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg 4261 cctggtatga gtcagcaaca ccttcttcac gaggcagacc tcagcgcccc cccccccctg 4321 caggtcgatc tggtaacccc agcgcggttg ctaccaagta gtgacccgct tcgtgatgca 4381 aaatccgctg acgatattcg ggcgatcgct gctgaatgcc atcgagcagt aacgtggcac 4441 cccgcccctg ccaagtcacc gcatccagac tgaacagcac caagaggcta aaacccaatc 4501 ccgccggtag cagcggagaa ctacccagca ttggtcccac caaagctaat gccgtcgtgg 4561 taaaaatcgc gatcgccgtc agactcaagc ccagttcgct catgcttcct catctaggtc 4621 acagtcttcg gcgatcgcat cgatctgatg ctgcagcaag cgttttccat accggcgatc 4681 gcgccgtcgc cctttcgctg ccgtggcccg cttacgagct cgtttatcga ccacgatcgc 4741 atccaaatcc gcgatcgctt cccagtccgg caattcagtc tggggcgtcc gtttcattaa 4801 tcctgatcag gcacgaaatt gctgtgcgta gtatcgcgca tagcggccag cctctgccaa 4861 cagcgcatcg tgattgcctg cctcaacaat ctggccgcgc tccatcacca agatgcggct 4921 ggcattacga accgtagcca gacggtgagc aatgataaag accgtccgtc cctgcatcac 4981 ccgttctagg gcctcttgca ccaaggtttc ggactcggaa tcaagcgccg aagtcgcctc 5041 atccagaatt aaaatgcgtg gatcctctac gccggacgca tcgtggccgg catcaccggc 5101 gccacaggtg cggttgctgg cgcctatatc gccgacatca ccgatgggga agatcgggct 5161 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc 5221 gggggactgt tgggcgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac 5281 ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg agagcgtcga 5341 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 5401 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 5461 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 5521 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc 5581 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct 5641 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct 5701 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga 5761 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat 5821 cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt 5881 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc 5941 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc 6001 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct 6061 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc 6121 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta 6181 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga 6241 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc 6301 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc 6361 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc 6421 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta 6481 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc 6541 ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca 6601 tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca 6661 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa 6721 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac 6781 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 6841 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 6901 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 6961 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 7021 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 7621 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 7681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 7741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 7801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 7861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 7921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 7981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8281 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 8581 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 8641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 8821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 8881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 8941 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 9001 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa tt //