LOCUS BBa_M36096 4803 bp DNA linear 29-Nov-2024 DEFINITION Mammalian actuator test rig ACCESSION BBa_M36096 VERSION BBa_M36096.1 FEATURES Location/Qualifiers misc_feature 1..425 /label=rpo_bla transcription terminator promoter 507..1108 /label=CMV promoter misc_feature 1119..1188 /label=student actuators go here polyA_site 1195..1320 /label=BGH polyA promoter 1432..1775 /label=SV40 promoter CDS 1886..2485 /label=puromycinR polyA_site 2613..2743 /label=SV40 polyA CDS 2789..3649 /label=AmpR promoter 3650..3827 /label=Pamp misc_feature 3828..4632 /label=pUCori ORIGIN 1 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg 61 cgggtttttt tatgggggga gtttagggaa agagcatttg tcagaatatt taagggcgcc 121 tgtcactttg cttgatatat gagaattatt taaccttata aatgagaaaa aagcaacgca 181 ctttaaataa gatacgttgc tttttcgatt gatgaacacc tataattaaa ctattcatct 241 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat 301 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg 361 ataatacaaa atataataca aactataaga tgttatcagt atttattatc atttagaata 421 aattttgtgt cgcccttaat tgtgagcgga taacaattac gagcttcatg cacagtggcg 481 ttgacattga ttattgacta gtggggatgc attagttatt aatagtaatc aattacgggg 541 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 601 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 661 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 721 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 781 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 841 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 901 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 961 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 1021 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 1081 ggtttagtga accgtcagat ccgctagcaa aagctagcac tgactgatcg atcgatcgat 1141 cgatcgatcg atccgatcga tccgaataga taagactaga ctacatacct cgagaaaatc 1201 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 1261 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 1321 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg 1381 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga 1441 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1501 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 1561 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 1621 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 1681 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag 1741 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt 1801 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata 1861 atacgacaag gtgaggaact aaaccatgac cgagtacaag cccacggtgc gcctcgccac 1921 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 1981 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 2041 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 2101 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 2161 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct 2221 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 2281 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 2341 gcgcgccggg gtgcccgcct tcctggagac atccgcgccc cgcaacctcc ccttctacga 2401 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 2461 catgacccgc aagcccggtg cctgacacgt gctacgagat ttcgattcca ccgccgcctt 2521 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg 2581 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg 2641 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 2701 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc 2761 tagctagagc ttggcgtaat catggtcatt accaatgctt aatcagtgag gcacctatct 2821 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 2881 cgatacggga gggcttacca tctggcccca gcgctgcgat gataccgcga gaaccacgct 2941 caccggctcc ggatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 3001 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 3061 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat cgctacaggc atcgtggtgt 3121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 3181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 3241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 3301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 3361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 3421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 3481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 3541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 3601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ttcttccttt 3661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 3721 gtatttagaa aaataaacaa ataggggtca gtgttacaac caattaacca attctgaaca 3781 ttatcgcgag cccatttata cctgaatatg gctcataaca ccccttgctc atgaccaaaa 3841 tcccttaacg tgagttacgc gcgcgtcgtt ccactgagcg tcagaccccg tagaaaagat 3901 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa 3961 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa 4021 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt 4081 agcccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt 4141 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata 4201 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt 4261 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac 4321 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga 4381 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg 4441 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa 4501 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat 4561 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc 4621 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga 4681 aggcgagagt agggaactgc caggcatcaa actaagcaga aggcccctga cggatggcct 4741 ttttgcgttt ctacaaactc tttctgtgtt gtaaaacgac ggccagtctt aagctcgggc 4801 ccc //