LOCUS BBa_M36098 4667 bp DNA linear 22-Nov-2024 DEFINITION E. coli actuator test rig ACCESSION BBa_M36098 VERSION BBa_M36098.1 FEATURES Location/Qualifiers CDS 1..1962 /label=rhaR and rhaS regulatory proteins promoter 1963..2038 /label=rhaBAD promoter misc_feature 2039..2094 /label=Student actuators go here misc_feature 2095..3272 /label=terminator and pUCori promoter 3273..3400 /label=Pamp CDS 3401..4261 /label=ampR ORIGIN 1 ttaatctttc tgcgaattga gatgacgcca ctggctgggc gtcatcccgg tttcccgggt 61 aaacaccacc gaaaaatagt tactatcttc aaagccacat tcggtcgaaa tatcactgat 121 taacaggcgg ctatgctgga gaagatattg cgcatgacac actctgacct gtcgcagata 181 ttgattgatg gtcattccag tctgctggcg aaattgctga cgcaaaacgc gctcactgca 241 cgatgcctca tcacaaaatt tatccagcgc aaagggactt ttcaggctag ccgccagccg 301 ggtaatcagc ttatccagca acgtttcgct ggatgttggc ggcaacgaat cactggtgta 361 acgatggcga ttcagcaaca tcaccaactg cccgaacagc aactcagcca tttcgttagc 421 aaacggcaca tgctgactac tttcatgctc aagctgacca ataacctgcc gcgcctgcgc 481 catccccatg ctacctaagc gccagtgtgg ttgccctgcg ctggcgttaa atcccggaat 541 cgccccctgc cagtcaagat tcagcttcag acgctccggg caataaataa tattctgcaa 601 aaccagatcg ttaacggaag cgtaggagtg tttatcatca gcatgaatgt aaaagagatc 661 gccacgggta atgcgataag ggcgatcgtt gagtacatgc aggccattac cgcgccagac 721 aatcaccagc tcacaaaaat catgtgtatg ttcagcaaag acatcttgcg gataacggtc 781 agccacagcg actgcctgct ggtcgctggc aaaaaaatca tctttgagaa gttttaactg 841 atgcgccacc gtggctacct cggccagaga acgaagttga ttattcgcaa tatggcgtac 901 aaatacgttg agaagattcg cgttattgca gaaagccatc ccgtccctgg cgaatatcac 961 gcggtgacca gttaaactct cggcgaaaaa gcgtcgaaaa gtggttactg tcgctgaatc 1021 cacagcgata ggcgatgtca gtaacgctgg cctcgctgtg gcgtagcaga tgtcgggctt 1081 tcatcagtcg caggcggttc aggtatcgct gaggcgtcag tcccgtttgc tgcttaagct 1141 gccgatgtag cgtacgcagt gaaagagaaa attgatccgc cacggcatcc caattcacct 1201 catcggcaaa atggtcctcc agccaggcca gaagcaagtt gagacgtgat gcgctgtttt 1261 ccaggttctc ctgcaaactg cttttacgca gcaagagcag taattgcata aacaagatct 1321 cgcgactggc ggtcgagggt aaatcatttt ccccttcctg ctgttccatc tgtgcaacca 1381 gctgtcgcac ctgctgcaat acgctgtggt taacgcgcca gtgagacgga tactgcccat 1441 ccagctcttg tggcagcaac tgattcagcc cggcgagaaa ctgaaatcga tccggcgagc 1501 gatacagcac attggtcaga cacagattat cggtatgttc atacagatgc cgatcatgat 1561 cgcgtacgaa acagaccgtg ccaccggtga tggtataggg ctgcccatta aacacatgaa 1621 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat 1681 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca 1741 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca 1801 cgaggtcagg ttcttacctt aaattttcga cggaaaacca cgtaaaaaac gtcgattttt 1861 caagatacag cgtgaatttt caggaaatgc ggtgagcatc acatcaccac aattcagcaa 1921 attgtgaaca tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt 1981 aacgagaagg tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcaggaggtg 2041 gtcgactcta gaggatcccc gcgccctcat ccgaaagggc gtattggtac cgagctcgaa 2101 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 2161 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 2221 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 2281 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 2341 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 2401 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 2461 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 2521 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 2581 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 2641 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 2701 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 2761 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 2821 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 2881 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 2941 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3001 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3061 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3121 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 3181 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 3241 taaagtatat atgagtaaac ttggtctgac agtcagaatt ggttaattgg ttgtaacact 3301 gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata 3361 accctgataa atgcttcaat aatattgaaa aaggaagaat atgagtattc aacatttccg 3421 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac 3481 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 3541 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat 3601 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga 3661 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac 3721 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat 3781 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 3841 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct 3901 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcga tggcaacaac 3961 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga 4021 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg 4081 gtttattgct gataaatccg gagccggtga gcgtggttct cgcggtatca tcgcagcgct 4141 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac 4201 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta 4261 atcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 4321 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 4381 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 4441 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc 4501 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta 4561 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg 4621 ttttcccagt cacgacgttg taaaacgacg gccagtgcca agcttaa //