LOCUS BBa_M36099 3638 bp DNA linear 16-Dec-2024 DEFINITION E. coli sensor test rig ACCESSION BBa_M36099 VERSION BBa_M36099.1 FEATURES Location/Qualifiers CDS 1..861 /label=ampR misc_feature 900..1704 /label=pUCori misc_feature 1705..2313 /label=rpo_bla transcription terminator misc_feature 2373..2434 /label=student sensors go here RBS 2435..2448 /label=RBS CDS 2449..3162 /label=Comet GFP stem_loop 3222..3269 /label=T7 terminator misc_feature 3270..3460 /label=rrnB1T1T2 transcripton terminator misc_feature 2314..2372 /label=cloning cassette 1 misc_feature 3163..3221 /label=cloning cassette 2 promoter 3461..3638 /label=Pamp ORIGIN 1 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 61 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 121 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 181 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 241 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 301 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 361 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 421 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 481 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 541 cctgtagcga tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 601 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 661 tcggcccttc cggctggctg gtttattgct gataaatccg gagccggtga gcgtggttct 721 cgcggtatca tcgcagcgct ggggccagat ggtaagccct cccgtatcgt agttatctac 781 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 841 tcactgatta agcattggta aaagcagagc attacgctga cttgacggga cggcgcaagc 901 tcatgaccaa aatcccttaa cgtgagttac gcgcgcgtcg ttccactgag cgtcagaccc 961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 1021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 1081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 1141 gtagccgtag ttagcccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 1201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 1261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 1321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 1381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 1441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 1501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 1561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 1621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 1681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 1741 cgaggaagcg gaaggcgaga gtagggaact gccaggcatc aaactaagca gaaggcccct 1801 gacggatggc ctttttgcgt ttctacaaac tctttctgtg ttgtaaaacg acggccagtc 1861 ttaagctcgg gccccctggg cggttctgat aacgagtaat cgttaatccg caaataacgt 1921 aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaagagc atttgtcaga 1981 atatttaagg gcgcctgtca ctttgcttga tatatgagaa ttatttaacc ttataaatga 2041 gaaaaaagca acgcacttta aataagatac gttgcttttt cgattgatga acacctataa 2101 ttaaactatt catctattat ttatgatttt ttgtatatac aatatttcta gtttgttaaa 2161 gagaattaag aaaataaatc tcgaaaataa taaagggaaa atcagttttt gatatcaaaa 2221 ttatacatgt caacgataat acaaaatata atacaaacta taagatgtta tcagtattta 2281 ttatgcattt agaataaatt ttgtgtcgcc cttattcgac tcactataga agttcctatt 2341 ctctagaaag tataggaact tcacttcatt ttgatcgatc gatcagatag tagtagtagt 2401 agatgtagta tatgatgatg tagtatgagt tagtaggaga taaaacatat gacggcattg 2461 acggaaggcg caaaattgtt cgaaaaagaa atcccataca tcaccgaact ggaaggcgat 2521 gttgaaggta tgaagttcat cattaagggt gagggcaccg gcgatgcaac tacgggcacc 2581 attaaagcga agtatatctg caccaccggt gacgttccgg tgccgtggag cacgctggtc 2641 accaccctga cctatggcgc gcagtgtttc gcgaagtacg gtccggaact gaaggacttc 2701 tataagagct gtatgcctga gggctatgtt caggagcgta ccattacctt tgagggtgat 2761 ggtgtcttta agacgcgtgc tgaggtgacc tttgagaatg gttccgtgta caatcgcgtg 2821 aaactgaatg gtcaaggttt taagaaagat ggtcacgtgc tgggcaaaaa cctggagttt 2881 aactttactc cgcattgcct gtgcatttgg ggcgaccaag cgaaccacgg tctgaaaagc 2941 gcgttcaaga ttatgcacga gattacgggt agcaaagagg acttcatcgt ggccgaccac 3001 acgcagatga acaccccgat cggtggcggt ccggtccatg tcccggagta ccaccacttg 3061 accgtttgga cctctttcgg taaagacccg gatgatgacg aaacggatca tctgaatatt 3121 gttgaggtta tcaaagccgt cgacctggaa acttaccgtt aaccccactt cagaagttcc 3181 tatactttct agagaatagg aacttcacta tagagtcgaa tctagcataa ccccttgggg 3241 cctctaaacg ggtcttgagg ggttttttga agggcgacac ccctcaatta gcccgggcga 3301 aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct 3361 cgcatgggga gtccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat 3421 ggggtcaggt gggaccaccg cgctactgcc gccaggcaaa caaggggtgt tatgagccat 3481 attcaggtat aaatgggctc gcgataatgt tcagaattgg ttaattggtt gtaacactga 3541 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 3601 cctgataaat gcttcaataa tattgaaaaa ggaagaat //