BBa_M36099 1 BBa_M36099 E. coli sensor test rig 2013-04-22T11:00:00Z 2015-05-08T01:14:03Z Gemini coding sequence sourced from Dr. Drew Endy and Martin et al., PlosOne 2009. The rest of the vector is sourced from DNA2.0. E. coli test vector for sensors. Has a Gemini protein coding sequence which produces a LacZalpha-GFP fusion protein. Comes with high copy number origin and ampicillin resistance; can replace with low copy number origin and kanamycin resistance if ordering from DNA 2.0. false false _848_ 0 10436 9 Not in stock true None false Joseph Shih annotation2215545 1 cloning cassette 1 range2215545 1 2314 2372 annotation2215539 1 rpo_bla transcription terminator range2215539 1 1705 2313 annotation2215542 1 Comet GFP range2215542 1 2449 3162 annotation2215544 1 rrnB1T1T2 transcripton terminator range2215544 1 3270 3460 annotation2215538 1 pUCori range2215538 1 900 1704 annotation2215537 1 ampR range2215537 1 1 861 annotation2215540 1 student sensors go here range2215540 1 2373 2434 annotation2215541 1 RBS range2215541 1 2435 2448 annotation2215543 1 T7 terminator range2215543 1 3222 3269 annotation2215546 1 cloning cassette 2 range2215546 1 3163 3221 annotation2215547 1 Pamp range2215547 1 3461 3638 BBa_M36099_sequence 1 atgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcgatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatccggagccggtgagcgtggttctcgcggtatcatcgcagcgctggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaaaagcagagcattacgctgacttgacgggacggcgcaagctcatgaccaaaatcccttaacgtgagttacgcgcgcgtcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttagcccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaggcgagagtagggaactgccaggcatcaaactaagcagaaggcccctgacggatggcctttttgcgtttctacaaactctttctgtgttgtaaaacgacggccagtcttaagctcgggccccctgggcggttctgataacgagtaatcgttaatccgcaaataacgtaaaaacccgcttcggcgggtttttttatggggggagtttagggaaagagcatttgtcagaatatttaagggcgcctgtcactttgcttgatatatgagaattatttaaccttataaatgagaaaaaagcaacgcactttaaataagatacgttgctttttcgattgatgaacacctataattaaactattcatctattatttatgattttttgtatatacaatatttctagtttgttaaagagaattaagaaaataaatctcgaaaataataaagggaaaatcagtttttgatatcaaaattatacatgtcaacgataatacaaaatataatacaaactataagatgttatcagtatttattatgcatttagaataaattttgtgtcgcccttattcgactcactatagaagttcctattctctagaaagtataggaacttcacttcattttgatcgatcgatcagatagtagtagtagtagatgtagtatatgatgatgtagtatgagttagtaggagataaaacatatgacggcattgacggaaggcgcaaaattgttcgaaaaagaaatcccatacatcaccgaactggaaggcgatgttgaaggtatgaagttcatcattaagggtgagggcaccggcgatgcaactacgggcaccattaaagcgaagtatatctgcaccaccggtgacgttccggtgccgtggagcacgctggtcaccaccctgacctatggcgcgcagtgtttcgcgaagtacggtccggaactgaaggacttctataagagctgtatgcctgagggctatgttcaggagcgtaccattacctttgagggtgatggtgtctttaagacgcgtgctgaggtgacctttgagaatggttccgtgtacaatcgcgtgaaactgaatggtcaaggttttaagaaagatggtcacgtgctgggcaaaaacctggagtttaactttactccgcattgcctgtgcatttggggcgaccaagcgaaccacggtctgaaaagcgcgttcaagattatgcacgagattacgggtagcaaagaggacttcatcgtggccgaccacacgcagatgaacaccccgatcggtggcggtccggtccatgtcccggagtaccaccacttgaccgtttggacctctttcggtaaagacccggatgatgacgaaacggatcatctgaatattgttgaggttatcaaagccgtcgacctggaaacttaccgttaaccccacttcagaagttcctatactttctagagaataggaacttcactatagagtcgaatctagcataaccccttggggcctctaaacgggtcttgaggggttttttgaagggcgacacccctcaattagcccgggcgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagtccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaacaaggggtgttatgagccatattcaggtataaatgggctcgcgataatgttcagaattggttaattggttgtaacactgacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagaat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z