BBa_M36009
1
BBa_M36009
5' Bicistronic UTR (strong), does not include ATG start
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
c/o Vivek Mutalik et al. BIOFAB Emeryville
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
please see long description.
false
Drew Endy
BBa_M36103
1
BBa_M36103
prokaryotic HY5 protein gene sequence
2011-05-02T11:00:00Z
2015-05-08T01:14:03Z
Arabidopsis
This part is the HY5 eukaryotic protein modified to be expressed in prokaryotes. The DNA sequence has been translated from the amino acids to be most compatible with prokaryotic DNA transcription.
false
false
_848_
0
9548
9
Not in stock
false
Correctly translating the HY5 amino acid sequence to DNA nucleotides that would be compatible with prokaryotic machinery.
false
Andee Wallace, Taylor Nguyen, Chad Viergever
annotation2119125
1
start codon
range2119125
1
1
3
annotation2119126
1
stop codon
range2119126
1
508
510
BBa_M36104
1
BBa_M36104
protein generator- promoter, UTR, HY5 gene
2011-05-02T11:00:00Z
2015-05-08T01:14:03Z
5' UTR and terminator sequence are provided by Drew Endy for the purposes of this class. The HY5 sequence is from Arabidopsis.
Composite part that includes a 5' UTR upstream of the HY5 DNA sequence, and a terminator sequence.
true
false
_848_
0
9548
9
Discontinued
false
Correct ordering of parts.
false
Andee Wallace, Taylor Nguyen, Chad Viergever
component2118888
1
BBa_M36103
component2118887
1
BBa_M36009
component2118886
1
BBa_J23100
component2118889
1
BBa_M36010
annotation2118887
1
BBa_M36009
range2118887
1
36
82
annotation2118888
1
BBa_M36103
range2118888
1
83
592
annotation2118889
1
BBa_M36010
range2118889
1
593
674
annotation2118886
1
BBa_J23100
range2118886
1
1
35
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_M36010
1
BBa_M36010
Transcription Terminator (Strong)
2011-04-25T11:00:00Z
2015-05-08T01:14:02Z
Guillaume Cambray et al. c/o BIOFAB Emeryville
E. coli RNA pol transcription terminator designed and tested by Guillaume Cambray at BIOFAB Emeryville. Based on the natural E. coli rnpB T1 terminator.
false
true
_848_
0
52
432
Not in stock
false
please see long description
false
Drew Endy
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_M36103_sequence
1
atgcaggaacaggcgaccagcagcctggcggcgagcagcctgccgagcagcagcgaacgcagcagcagcagcgcgccgcatctggaaattaaagaaggcattgaaagcgatgaagaaattcgccgcgtgccggaatttgcgggcggcgaagcggtgggcaaagaaaccagcggccgcgaaagcggcagcgcgaccggccaggaacgcacccaggcgaccgtgggcgaaagccagcgcaaacgcggccgcaccccggcggaaaaagaaaacaaacgcctgaaacgcctgctgcgcaaccgcgtgagcgcgcagcaggcgcgcgaacgcaaaaaagcgtatctgagcgaactggaaaaccgcgtgaaagatctggaaaacaaaaacagcgaactggaagaacgcctgagcaccctgcagaacgaaaaccagatgctgcgccatattctgaaaaacaccaccggcaacaaacgcggcggcggcggcggcagcaacgcggatgcgagcctgtaa
BBa_M36010_sequence
1
tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36104_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctatagagggtattaataatgtatggattaaaaggggaggtataacaaatgcaggaacaggcgaccagcagcctggcggcgagcagcctgccgagcagcagcgaacgcagcagcagcagcgcgccgcatctggaaattaaagaaggcattgaaagcgatgaagaaattcgccgcgtgccggaatttgcgggcggcgaagcggtgggcaaagaaaccagcggccgcgaaagcggcagcgcgaccggccaggaacgcacccaggcgaccgtgggcgaaagccagcgcaaacgcggccgcaccccggcggaaaaagaaaacaaacgcctgaaacgcctgctgcgcaaccgcgtgagcgcgcagcaggcgcgcgaacgcaaaaaagcgtatctgagcgaactggaaaaccgcgtgaaagatctggaaaacaaaaacagcgaactggaagaacgcctgagcaccctgcagaacgaaaaccagatgctgcgccatattctgaaaaacaccaccggcaacaaacgcggcggcggcggcggcagcaacgcggatgcgagcctgtaatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
BBa_M36009_sequence
1
tatagagggtattaataatgtatggattaaaaggggaggtataacaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z