BBa_M36118 1 BBa_M36118 GULO enzyme with start and stop 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z mouse This construct includes the kozacs sequence, the sequence for the L-gulonolactone oxidase enzyme, and a stop codon. This is a standalone part, ie, if this is the only enzyme you want to produce, then this sequences will allow you to do this with minimal work. true false _848_ 0 13354 9 Discontinued false sequence was slightly changed (silent mutation) from published work in order to allow for the use of restriction enzymes. false Samson Phan, Noriko Ishisoko component2176027 1 BBa_M36115 component2176028 1 BBa_M36116 component2176029 1 BBa_M36117 annotation2176027 1 BBa_M36115 range2176027 1 1 9 annotation2176028 1 BBa_M36116 range2176028 1 18 1334 annotation2176029 1 BBa_M36117 range2176029 1 1343 1345 BBa_M36117 1 BBa_M36117 Stop Codon 2012-05-28T11:00:00Z 2015-05-08T01:14:03Z NA Stop Codon false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_M36115 1 BBa_M36115 Kozak consensus sequence 2012-05-27T11:00:00Z 2015-05-08T01:14:03Z NA Kozak consensus sequence initiates translation in eukaryotes false false _848_ 0 13354 9 Not in stock false includes start codon false Samson Phan, Noriko Ishisoko BBa_M36116 1 BBa_M36116 L-gulonolactone oxidase (GULO) enzyme 2012-05-27T11:00:00Z 2015-05-08T01:14:03Z Functional rescue of vitamin C synthesis deficiency in human cells using adenoviral-based expression of murine L-gulono-g-lactone oxidase, 2003 L-gulonolactone oxidase is the final step of the biosynthetic pathway for ascorbic acid creation. Lost in human mammalian cells 63 million years ago. false false _848_ 0 13354 9 Not in stock false NA false Samson Phan, Noriko Ishisoko BBa_M36117_sequence 1 taa BBa_M36115_sequence 1 gccgccatg BBa_M36118_sequence 1 gccgccatgtactagaggtgcacggctacaaaggcgtgcagtttcagaactgggccaaaacctacgggtgctctccagagatgtattatcaacccacgagcgtgggggaggtgcgagaagtgctggcccttgctaggcagcagaacaaaaaggtcaaggtggttggtggcgggcattcaccttccgatatagcctgcacagatggatttatgattcacatgggaaagatgaatcgggtgctccaagtggataaggaaaaaaaacaggtgacggtggaagcggggatcctgctgacagacctgcatcctcagttagacaaacacgggctggccctttctaatctgggggccgtttccgatgtgactgtcggtggcgttatcggcagcggcacgcacaatacgggtatcaagcacggtatccttgccacacaagtggtagctcttaccctcatgaaagcagacggcacggtgctggaatgctctgaatcctcgaacgccgatgtattccaagcggctagagtgcacctaggttgcctgggagtcattctgacggttaccctgcagtgtgttccacagtttcatttattagagacttcattcccttcaacactgaaagaagtcttggataatctggattcacacttgaagaagtctgagtattttcgattcttatggttccctcatagtgagaacgtgagcatcatctatcaggatcatacgaataaggaaccgtcatccgcctcaaattggttctgggattatgccatcgggttctacctgttggagttcttgttatggactagcacctatctgccgcgccttgtgggatggattaatcgttttttcttttggttgctgtttaattgcaaaaaggagtcgtccaacctgagtcataagattttttcgtatgagtgcaggtttaagcaacatgtacaggattgggctattccgcgagagaagaccaaagaagcgctcttagagttgaaagctatgctcgaggcccatccaaaagtcgttgcccactacccagtggaggtgcgctttactagaggggacgacatactgcttagcccttgtttccagagagattcctgttatatgaatatcataatgtatcggccctatgggaaagatgtccccaggttggattactggctggcttacgaaaccataatgaaaaaattcggcgggcggccacactgggccaaggcgcataattgcacccgaaaagattttgaaaaaatgtatcctgcttttcataagttttgcgatatccgagagaagctggatcctaccgggatgtttctgaattcctatctcgagaaagtgttttactactagagtaa BBa_M36116_sequence 1 gtgcacggctacaaaggcgtgcagtttcagaactgggccaaaacctacgggtgctctccagagatgtattatcaacccacgagcgtgggggaggtgcgagaagtgctggcccttgctaggcagcagaacaaaaaggtcaaggtggttggtggcgggcattcaccttccgatatagcctgcacagatggatttatgattcacatgggaaagatgaatcgggtgctccaagtggataaggaaaaaaaacaggtgacggtggaagcggggatcctgctgacagacctgcatcctcagttagacaaacacgggctggccctttctaatctgggggccgtttccgatgtgactgtcggtggcgttatcggcagcggcacgcacaatacgggtatcaagcacggtatccttgccacacaagtggtagctcttaccctcatgaaagcagacggcacggtgctggaatgctctgaatcctcgaacgccgatgtattccaagcggctagagtgcacctaggttgcctgggagtcattctgacggttaccctgcagtgtgttccacagtttcatttattagagacttcattcccttcaacactgaaagaagtcttggataatctggattcacacttgaagaagtctgagtattttcgattcttatggttccctcatagtgagaacgtgagcatcatctatcaggatcatacgaataaggaaccgtcatccgcctcaaattggttctgggattatgccatcgggttctacctgttggagttcttgttatggactagcacctatctgccgcgccttgtgggatggattaatcgttttttcttttggttgctgtttaattgcaaaaaggagtcgtccaacctgagtcataagattttttcgtatgagtgcaggtttaagcaacatgtacaggattgggctattccgcgagagaagaccaaagaagcgctcttagagttgaaagctatgctcgaggcccatccaaaagtcgttgcccactacccagtggaggtgcgctttactagaggggacgacatactgcttagcccttgtttccagagagattcctgttatatgaatatcataatgtatcggccctatgggaaagatgtccccaggttggattactggctggcttacgaaaccataatgaaaaaattcggcgggcggccacactgggccaaggcgcataattgcacccgaaaagattttgaaaaaatgtatcctgcttttcataagttttgcgatatccgagagaagctggatcctaccgggatgtttctgaattcctatctcgagaaagtgttttac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z