BBa_M36118
1
BBa_M36118
GULO enzyme with start and stop
2012-05-28T11:00:00Z
2015-05-08T01:14:03Z
mouse
This construct includes the kozacs sequence, the sequence for the L-gulonolactone oxidase enzyme, and a stop codon. This is a standalone part, ie, if this is the only enzyme you want to produce, then this sequences will allow you to do this with minimal work.
true
false
_848_
0
13354
9
Discontinued
false
sequence was slightly changed (silent mutation) from published work in order to allow for the use of restriction enzymes.
false
Samson Phan, Noriko Ishisoko
component2176027
1
BBa_M36115
component2176028
1
BBa_M36116
component2176029
1
BBa_M36117
annotation2176027
1
BBa_M36115
range2176027
1
1
9
annotation2176028
1
BBa_M36116
range2176028
1
18
1334
annotation2176029
1
BBa_M36117
range2176029
1
1343
1345
BBa_M36117
1
BBa_M36117
Stop Codon
2012-05-28T11:00:00Z
2015-05-08T01:14:03Z
NA
Stop Codon
false
false
_848_
0
13354
9
Not in stock
false
NA
false
Samson Phan, Noriko Ishisoko
BBa_M36115
1
BBa_M36115
Kozak consensus sequence
2012-05-27T11:00:00Z
2015-05-08T01:14:03Z
NA
Kozak consensus sequence initiates translation in eukaryotes
false
false
_848_
0
13354
9
Not in stock
false
includes start codon
false
Samson Phan, Noriko Ishisoko
BBa_M36116
1
BBa_M36116
L-gulonolactone oxidase (GULO) enzyme
2012-05-27T11:00:00Z
2015-05-08T01:14:03Z
Functional rescue of vitamin C synthesis deficiency in human cells using
adenoviral-based expression of murine L-gulono-g-lactone oxidase, 2003
L-gulonolactone oxidase is the final step of the biosynthetic pathway for ascorbic acid creation. Lost in human mammalian cells 63 million years ago.
false
false
_848_
0
13354
9
Not in stock
false
NA
false
Samson Phan, Noriko Ishisoko
BBa_M36117_sequence
1
taa
BBa_M36115_sequence
1
gccgccatg
BBa_M36118_sequence
1
gccgccatgtactagaggtgcacggctacaaaggcgtgcagtttcagaactgggccaaaacctacgggtgctctccagagatgtattatcaacccacgagcgtgggggaggtgcgagaagtgctggcccttgctaggcagcagaacaaaaaggtcaaggtggttggtggcgggcattcaccttccgatatagcctgcacagatggatttatgattcacatgggaaagatgaatcgggtgctccaagtggataaggaaaaaaaacaggtgacggtggaagcggggatcctgctgacagacctgcatcctcagttagacaaacacgggctggccctttctaatctgggggccgtttccgatgtgactgtcggtggcgttatcggcagcggcacgcacaatacgggtatcaagcacggtatccttgccacacaagtggtagctcttaccctcatgaaagcagacggcacggtgctggaatgctctgaatcctcgaacgccgatgtattccaagcggctagagtgcacctaggttgcctgggagtcattctgacggttaccctgcagtgtgttccacagtttcatttattagagacttcattcccttcaacactgaaagaagtcttggataatctggattcacacttgaagaagtctgagtattttcgattcttatggttccctcatagtgagaacgtgagcatcatctatcaggatcatacgaataaggaaccgtcatccgcctcaaattggttctgggattatgccatcgggttctacctgttggagttcttgttatggactagcacctatctgccgcgccttgtgggatggattaatcgttttttcttttggttgctgtttaattgcaaaaaggagtcgtccaacctgagtcataagattttttcgtatgagtgcaggtttaagcaacatgtacaggattgggctattccgcgagagaagaccaaagaagcgctcttagagttgaaagctatgctcgaggcccatccaaaagtcgttgcccactacccagtggaggtgcgctttactagaggggacgacatactgcttagcccttgtttccagagagattcctgttatatgaatatcataatgtatcggccctatgggaaagatgtccccaggttggattactggctggcttacgaaaccataatgaaaaaattcggcgggcggccacactgggccaaggcgcataattgcacccgaaaagattttgaaaaaatgtatcctgcttttcataagttttgcgatatccgagagaagctggatcctaccgggatgtttctgaattcctatctcgagaaagtgttttactactagagtaa
BBa_M36116_sequence
1
gtgcacggctacaaaggcgtgcagtttcagaactgggccaaaacctacgggtgctctccagagatgtattatcaacccacgagcgtgggggaggtgcgagaagtgctggcccttgctaggcagcagaacaaaaaggtcaaggtggttggtggcgggcattcaccttccgatatagcctgcacagatggatttatgattcacatgggaaagatgaatcgggtgctccaagtggataaggaaaaaaaacaggtgacggtggaagcggggatcctgctgacagacctgcatcctcagttagacaaacacgggctggccctttctaatctgggggccgtttccgatgtgactgtcggtggcgttatcggcagcggcacgcacaatacgggtatcaagcacggtatccttgccacacaagtggtagctcttaccctcatgaaagcagacggcacggtgctggaatgctctgaatcctcgaacgccgatgtattccaagcggctagagtgcacctaggttgcctgggagtcattctgacggttaccctgcagtgtgttccacagtttcatttattagagacttcattcccttcaacactgaaagaagtcttggataatctggattcacacttgaagaagtctgagtattttcgattcttatggttccctcatagtgagaacgtgagcatcatctatcaggatcatacgaataaggaaccgtcatccgcctcaaattggttctgggattatgccatcgggttctacctgttggagttcttgttatggactagcacctatctgccgcgccttgtgggatggattaatcgttttttcttttggttgctgtttaattgcaaaaaggagtcgtccaacctgagtcataagattttttcgtatgagtgcaggtttaagcaacatgtacaggattgggctattccgcgagagaagaccaaagaagcgctcttagagttgaaagctatgctcgaggcccatccaaaagtcgttgcccactacccagtggaggtgcgctttactagaggggacgacatactgcttagcccttgtttccagagagattcctgttatatgaatatcataatgtatcggccctatgggaaagatgtccccaggttggattactggctggcttacgaaaccataatgaaaaaattcggcgggcggccacactgggccaaggcgcataattgcacccgaaaagattttgaaaaaatgtatcctgcttttcataagttttgcgatatccgagagaagctggatcctaccgggatgtttctgaattcctatctcgagaaagtgttttac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z