BBa_M36124 1 BBa_M36124 Glucose-6-Phosphate Isomerase for manipulation of glycolysis in yeast 2015-11-05T12:00:00Z 2015-11-05T09:22:52Z The part was derived from a uniprot sequence of S cerevisiae (baker's yeast), strain ATCC 204508 / S288c. Glucose-6-phosphate isomerase (Gis a naturally produced enzyme in most organisms that use glycolysis. It catalyzes the second step of the glycogenic cycle, which is the transformation of glucose-6-phosphate to fructose-6-phosphate. Overexpression of this enzyme in a vector with a strong constitutive promoter could lead to interesting metabolic effects and potentially increase the tolerance of the host organism to high concentrations of glucose. false false _848_ 29105 29105 9 false We edited the genetic sequence to remove repeats greater than 10 base pairs in length, so that it could be synthesized and replicated easily. All mutations introduced were silent to maintain an identical function of the wild type protein. This was to allow better comparison of the effects of overexpression. false Daniel Hart, Gustavo Garcia, Raymond Serrano annotation2478405 1 Silent mutation C to T range2478405 1 787 788 annotation2478407 1 Silent mutation A to G range2478407 1 1663 1663 annotation2478406 1 Silent mutation T to C range2478406 1 1543 1543 annotation2478404 1 Silent mutation A to G range2478404 1 493 493 annotation2478403 1 Silent mutation T to C range2478403 1 154 154 BBa_M36124_sequence 1 atggccgctttgaccagagacccacaatttcaaaagttgcaacaatggtacagagaacacagatctgaattaaacttgcgtagattgttcgatgctaataaggaccgtttcaaccacttctctttgaccttgaataccaaccacggtcatatcttggtcgattactccaagaacttagttaccgaagatgtcatgagaatgttagttgatttggccaagtccagaggtgttgaagctgctagagaaagaatgttcaacggtgaaaagattaattacaccgaaggtagagccgttttgcacgtcgctttgcgtaaccgttccaacaccccaattttggtcgatggtaaagatgttatgcctgaagttaacaaagttttggataaaatgaagtctttttgtcaacgtgtcagatctggtgactggaagggttacaccggtaagactatcaccgatgttatcaacattggtattggtggttccgacttgggtccgttgatggtcactgaggccttgaagccatactcctctggtggtccaagagtttggtacgtttctaacattgatggtactcacatcgccaagactttggctcaattaaacccagaatcttctttgtttatcattgcttctaaaactttcactacccaagaaactattactaatgctgaaactgccaaggaatggttcttacaagccgctaaggatccatctgctgtcgccaagcacttcgttgctttatccaccaacaccaccaaggttaaagaatttggtattgaccctcaaaatatgtttgaattctgggactgggttggtggtagatattctttgtggtccgctattggtttgtctattgccttgcacgttggtttcgataactttgaacaattattatctggtgctcactggatggaccaacactttagaaccactccattggaaaagaacgccccagtcttgttggctttgttgggtatctggtatattaactgtttcggttgtgaaactcacgccatgttgccatacgatcaatacttgcacagattcgctgcttacttccaacaaggtgatatggaatccaatggtaaatacatcactaagtccggtactagagttgaccaccaaaccggtccaattgtttggggtgaaccaggtactaacggtcaacatgctttttaccaattgattcatcaaggtactaagatgattccatgtgacttcttgatcccagttcaaactcaacacccaatcagaaagggtttacaccacaagattttattggctaacttcttagcccaaactgaggctttgatgagaggtaagtctactgaagaagctagaaaggagttgcaagctgccggtaagtctcctgaagatttggaaagattgttgccacataaggttttcgaaggtaaccgtccaactaactccattgtttttaccaagttaactccattcatgttgggtgctttggttgctatgtacgaacacaaaatcttcgtccaaggtatcatctgggatattaactccttcgaccaatggggcgttgaattgggtaagcaattggccaagaagattgaaccagaattggatggttccgctcaagtcacttctcatgatgcctctaccaacggtttgatcaactttatcaagcaacaaagagaggctagagttcaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z