BBa_M36125 1 BBa_M36125 d1 (RNA scaffold) 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z Unmodified sequence from the paper "Organization of Intracellular Reactions with Rationally Designed RNA Assemblies" (Science 2011, Delebecque, et. al). Encodes for part of a 1 dimensional self-assembling RNA scaffold. false false _848_ 0 13699 9 Not in stock false The sequence comes directly from the Delebecque Science paper. To fold into a functional RNA scaffold, it should be paired with the appropriate aptamers and terminator. false Daniel McHugh BBa_M36135 1 BBa_M36135 Transcription terminator (apFAB388) 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z BIOFAB: part apFAB388, construct pFAB822 Released HQ 2013 Strong terminator from BIOFAB's terminator project false false _848_ 0 13700 9 In stock false Length similar to terminators used in Delebecque et al., Science 2011 false Vir Choksi BBa_M36126 1 BBa_M36126 Theophylline responsive ribozyme 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z M. N. Win, C. D. Smolke, ???A modular and extensible RNA-based gene-regulatory platform for engineering cellular function??? PNAS 36, 14283-14288 (2007). Ribozyme that self-cleaves in the presence of theophylline. false false _848_ 0 13699 9 Not in stock false The part includes two brief spacer sequences to ensure functionality over a range of transcript contexts. false Daniel McHugh BBa_M36134 1 BBa_M36134 1D inducible RNA scaffold 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, ???Organization of intracellular reactions with rationally designed RNA assemblies??? Science 333, 470-474 (2011). M. N. Win, C. D. Smolke, ???A modular and extensible RNA-based gene-regulatory platform for engineering cellular function??? PNAS 36, 14283-14288 (2007). Self-assembles into a one-dimensional RNA scaffold with binding domains for the MS2 bacteriophage coat protein. When presented with theophylline, the ribozyme should cleave the scaffold sequences, releasing the aptamers from the existing scaffold and preventing other scaffolds from forming. false false _848_ 0 13699 9 Not in stock false The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. The PP7 aptamer was omitted to decrease the length of sequence not involved in scaffold folding. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts. false Daniel McHugh component2176084 1 BBa_M36129 component2176083 1 BBa_M36126 component2176082 1 BBa_M36125 component2176085 1 BBa_M36135 annotation2176083 1 BBa_M36126 range2176083 1 65 184 annotation2176085 1 BBa_M36135 range2176085 1 237 275 annotation2176084 1 BBa_M36129 range2176084 1 193 228 annotation2176082 1 BBa_M36125 range2176082 1 1 56 BBa_M36129 1 BBa_M36129 PP7 aptamer 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, ???Organization of intracellular reactions with rationally designed RNA assemblies??? Science 333, 470-474 (2011). Encodes for an RNA sequence which functions as the binding domain for the PP7 bacteriophage coat protein. false false _848_ 0 13699 9 Not in stock false Can be used in the context of the one- and two-dimensional RNA scaffolds described in the Delebecque paper. false Daniel McHugh BBa_M36126_sequence 1 aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaatgtcgaaaaagaaaaataaaaa BBa_M36129_sequence 1 gaattccgaccagaagatatggcttcggttgggttc BBa_M36135_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_M36134_sequence 1 taggcgcctagcctaatgtacattaagttatttttccggatgaatagaatatattctactagagaaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaatgtcgaaaaagaaaaataaaaatactagaggaattccgaccagaagatatggcttcggttgggttctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_M36125_sequence 1 taggcgcctagcctaatgtacattaagttatttttccggatgaatagaatatattc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z