BBa_M36125
1
BBa_M36125
d1 (RNA scaffold)
2012-05-31T11:00:00Z
2015-05-08T01:14:03Z
Unmodified sequence from the paper "Organization of Intracellular Reactions with Rationally Designed RNA Assemblies" (Science 2011, Delebecque, et. al).
Encodes for part of a 1 dimensional self-assembling RNA scaffold.
false
false
_848_
0
13699
9
Not in stock
false
The sequence comes directly from the Delebecque Science paper. To fold into a functional RNA scaffold, it should be paired with the appropriate aptamers and terminator.
false
Daniel McHugh
BBa_M36135
1
BBa_M36135
Transcription terminator (apFAB388)
2012-05-31T11:00:00Z
2015-05-08T01:14:03Z
BIOFAB: part apFAB388, construct pFAB822
Released HQ 2013
Strong terminator from BIOFAB's terminator project
false
false
_848_
0
13700
9
In stock
false
Length similar to terminators used in Delebecque et al., Science 2011
false
Vir Choksi
BBa_M36126
1
BBa_M36126
Theophylline responsive ribozyme
2012-05-31T11:00:00Z
2015-05-08T01:14:03Z
M. N. Win, C. D. Smolke, ???A modular and extensible RNA-based gene-regulatory platform for engineering cellular function??? PNAS 36, 14283-14288 (2007).
Ribozyme that self-cleaves in the presence of theophylline.
false
false
_848_
0
13699
9
Not in stock
false
The part includes two brief spacer sequences to ensure functionality over a range of transcript contexts.
false
Daniel McHugh
BBa_M36134
1
BBa_M36134
1D inducible RNA scaffold
2012-05-31T11:00:00Z
2015-05-08T01:14:03Z
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, ???Organization of intracellular reactions with rationally designed RNA assemblies??? Science 333, 470-474 (2011).
M. N. Win, C. D. Smolke, ???A modular and extensible RNA-based gene-regulatory platform for engineering cellular function??? PNAS 36, 14283-14288 (2007).
Self-assembles into a one-dimensional RNA scaffold with binding domains for the MS2 bacteriophage coat protein. When presented with theophylline, the ribozyme should cleave the scaffold sequences, releasing the aptamers from the existing scaffold and preventing other scaffolds from forming.
false
false
_848_
0
13699
9
Not in stock
false
The riboswitch was inserted into the scaffold sequence directly upstream of the aptamer. The PP7 aptamer was omitted to decrease the length of sequence not involved in scaffold folding. Spacers were used to prevent folding issues with the scaffold and ribozyme components of the transcripts.
false
Daniel McHugh
component2176084
1
BBa_M36129
component2176083
1
BBa_M36126
component2176082
1
BBa_M36125
component2176085
1
BBa_M36135
annotation2176083
1
BBa_M36126
range2176083
1
65
184
annotation2176085
1
BBa_M36135
range2176085
1
237
275
annotation2176084
1
BBa_M36129
range2176084
1
193
228
annotation2176082
1
BBa_M36125
range2176082
1
1
56
BBa_M36129
1
BBa_M36129
PP7 aptamer
2012-05-31T11:00:00Z
2015-05-08T01:14:03Z
C. J. Delebecque, A. B. Lindner, P. A. Silver, F. A. Aldaye, ???Organization of intracellular reactions with rationally designed RNA assemblies??? Science 333, 470-474 (2011).
Encodes for an RNA sequence which functions as the binding domain for the PP7 bacteriophage coat protein.
false
false
_848_
0
13699
9
Not in stock
false
Can be used in the context of the one- and two-dimensional RNA scaffolds described in the Delebecque paper.
false
Daniel McHugh
BBa_M36126_sequence
1
aaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaatgtcgaaaaagaaaaataaaaa
BBa_M36129_sequence
1
gaattccgaccagaagatatggcttcggttgggttc
BBa_M36135_sequence
1
aaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_M36134_sequence
1
taggcgcctagcctaatgtacattaagttatttttccggatgaatagaatatattctactagagaaacaaacaaagctgtcaccggatgtgctttccggtctgatgagtccgtgataccagcatcgtcttgatgcccttggcagcagtggacgaggacgaaatgtcgaaaaagaaaaataaaaatactagaggaattccgaccagaagatatggcttcggttgggttctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
BBa_M36125_sequence
1
taggcgcctagcctaatgtacattaagttatttttccggatgaatagaatatattc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z