BBa_M36162
1
ROSES
RhNUDX1 - Rose Scent (Geraniol) Precursor Catalyzing Gene
2015-10-21T11:00:00Z
2015-12-03T05:10:55Z
Sequence found by converting amino acid sequence of RhNUDX1 protein native to Rosa x hybrida roses (Papa Meilland breed).
Coding sequence for the enzyme RhNUDX1. This protein catalyzes the removal of a phosphate group from geranyl diphosphate to produce geranyl monophosphate (phosphatase is required to convert geranyl monophospate to geraniol, the actual scented compound).
false
false
_848_
29079
29079
9
true
This DNA sequence was created by converting the amino acid sequence of RhNUDX1 using the Genedesigner 2.0. We used the IDT Codon Optimizer tool to improve compatibility with E. coli, altering base pairs to preserve amino acid sequence. To remove restriction site problems with BsaI, we modified nucleotide 12 (G -> A) and nucleotide 33 (G -> A).
false
Luke Murphy, Julia Duncan, Bruce Tiu
annotation2478219
1
Replace G with A
range2478219
1
33
33
annotation2478428
1
Added TAG
range2478428
1
451
453
annotation2478218
1
Replace G with A
range2478218
1
12
12
BBa_M36162_sequence
1
atgggcaacgaaaccgtagtggtcgcggagacagccggcagcatcaaagtagcagtggtggtctgtttactgcgcggtcagaacgtcttactgggccgccgccgtagctcactgggcgattccaccttctcgttaccttcggggcacttggaattcggagaatcgtttgaagaatgcgcggcgcgcgaactgaaagaggaaaccgacctggatattggaaaaattgaactgctgacggttacaaataacctgtttctggatgaagcaaaaccctcacagtatgtggcagtgttcatgcgggctgtgctggcggatccgcggcaagaaccacaaaatattgaaccggaattttgcgacggttgggggtggtatgaatgggacaatctcccgaagccgttgttttggccgctggataatgtcgtccaggatggcttcaacccgtttcctacgtag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z