BBa_M36177
1
BBa_M36177
pH sensor
2015-10-22T11:00:00Z
2015-12-03T02:25:54Z
rcFB transcriptional regulator in Lactococcus lactis genome.
pH sensor, active within range 5.5-7.0. Our part is based on the acid inducible promoter region of a rcFB gene (procuding rcFB protein) in Lactococcus lactis. Ou assumptions are as follows: (1) generally Lactococcus lactis genes will work in E. coli, (2) all transcription factors specific to this rcFB gene are endogenous to E. coli, (3) there is no additional layer of feedback controlling the rcFB gene, (4) the rcFB gene is more responsive to lactic acid/lactate than carbohydrates (to which it also reacts), (5) base level of transcription is low in comparison to when gene is turned on.
false
false
_848_
29085
29099
9
false
We were limited by the following restrictions (1) 200-1500 bp size limit, (2) no Bbsl, Bsal, or BsmBI sites, (3) no repeats of length greater than 5 base pairs.
false
Majed Magzoub, Guhan Venkataraman, Joe Carpenter
annotation2478349
1
promoter
range2478349
1
1
431
BBa_M36177_sequence
1
tattggctttgttgtcttattaattgctggtggagccttcggtcttggttacggtggtgttgcaccatttggtcaagcaattgctattcggaggaggggagaatgattcaagtaaagaccgtatcggtgttgcaacttctaccttctttggatttttagatttaggtgttggtggaggacctattgttcttgggattggggggagcaattattccaatgcttggaaatggtatgcttggtttcagaaatttgtatctttacagtgctcccgcagttgtaattgtttggattatagggaaatactatctcattcatggtaaacatcaaaaatcaagttctaaagtttgattttaatgatttacataaaaaatgatataatgaaatggctagcagccttatataggcgaataaatgaacaaattagcgagtag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z