BBa_M36188
1
BBa_M36188
Ligninase Coding Region
2012-05-02T11:00:00Z
2015-05-08T01:14:03Z
MAFKQLFAAISLALLLSAANAAAVIQKRATCSNGKYVGDASCCAWFDVLDDIQNLFHGGQCGAEAHESIRLVFHDSIAISPAMEAQGKFGGGGADGSIMIFDDIETAFHPNIGLDEIVKLQKPFVQKHGVTPGDFIAFAGAVALSNCPGAPQMNFFTGRAPATQPAPDGLVPEPFHTVDQIINRVNDAGEFDELELVWMLSAHSVAAVNDVNPTVQGLPFDSTPGIFDSQFFVETQLRGTAFPGSGGNQGEVESPLPGEIRIQSDHTIARDSRTACEWQSFVNNQSKLVDDFQFIFLALTQLGQDPNAMTDCSDVIPQSKPIPGNLPFSFFPAGKTIKDVEQACAETPFPTLTTLPGPETSVQRIPPPPGA
This coding region has been created from Cullen et al "Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium." The sequence was modified so that all introns were removed and potential looping eliminated. Additionally, the amino acids have been chosen such that the tRNA ratios for e. coli has been optimized. Minimizing loops was done using Gene Designer and checking for tRNA in e.coli was accomplished using Optimizer.
The coding sequence is given seen here is not identical to the sequence given by Cullen et al. However, this sequence has been checked against the Blast database and been found to be highly homologous to multiple Ligninase Peroxidase Regions. GIven here is the Amino Acid Sequence.
false
false
_848_
0
13233
9
Not in stock
false
This enzyme works at pH 4.5 as found here: http://www.springerlink.com/content/7616k56005852420/fulltext.pdf?MUD=MP
Sanroman et al. "Optimum stability conditions of pH and temperature for ligninaseand manganese-dependent peroxidase from Phanerochaete chrysosporium. Application to in vitro decolorization of Poly R-478 by MnP." World Journal of Microbiology and Biotechnology. 22: 607-612. 2006.
false
Aaron Thayer
BBa_M36188_sequence
1
atggccttcaaacaactcttcgctgcaatttccctcgctctgctcttgtccgctgctaatgctgccgcagttatccagaaacgtgctacctgtagcaacggtaagtacgttggtgatgcaagctgctgcgcctggtttgatgtcctggatgatattcagaatctgtttcacggtggtcaatgcggtgcggaagcacacgagagcatccgcctggtgttccacgacagcattgcaatcagccctgcgatggaagcgcagggtaagttcggcggtggcggtgccgatggtagcatcatgatcttcgacgacatcgaaaccgccttccacccgaatatcggtctggacgagattgtgaaactgcaaaaaccgtttgtgcagaagcatggtgtaaccccaggtgacttcatcgcgtttgcaggtgcggtcgcgctgagcaactgcccgggtgcgccgcagatgaatttctttacgggccgtgctccggcgacgcagccagcaccagacggtttggtgccggaaccgtttcataccgttgaccagattatcaatcgtgtcaacgatgcaggcgagttcgacgagctggagttggtctggatgctgagcgcgcacagcgtcgcggcagtgaatgacgttaacccgactgtccaaggcttgccgtttgatagcacgccgggtattttcgacagccaattcttcgttgaaacccaactgcgcggcacggccttccctggtagcggtggcaatcagggtgaagtggaaagcccgctgccgggtgagatccgcattcagagcgaccatacgattgcgcgcgattcccgtacggcgtgcgagtggcagtctttcgttaacaatcagtcgaagctggtggatgactttcagttcatctttctggcgctgacccagctgggccaagatccgaacgcaatgacggactgtagcgacgtgatcccgcagagcaagccgatcccgggcaatctgcctttttctttcttccctgcgggtaaaaccattaaagacgttgagcaggcctgtgccgaaaccccgttcccaaccctgacgacgctgcctggcccggaaacgtcggtgcaacgtattccgccaccaccgggtgcc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z