BBa_M36192 1 BBa_M36192 Bicistronic Linker Sequence 2012-06-02T11:00:00Z 2015-05-08T01:14:03Z Aaron Thayer and Everett Frost custom designed this part. This is a custom designed linker sequence designed to fit between two protein coding domains in a bicistronic system. The linker contains multiple frameshifted TAA stop codons and is long enough to prevent ribosome interference. false false _848_ 0 13358 9 Not in stock false Synthesis was difficult due to the number of TAA repeats. false Everett Frost annotation2176101 1 Framshifted TAAs range2176101 1 1 101 BBa_M36191 1 BBa_M36191 Ligninase Coding Sequence 2012-06-02T11:00:00Z 2015-05-08T01:14:03Z ???Nucleotide Sequence of a ligninase gene from Phanerochaete Chrsosporium.??? Smith et al. Nucleic Acids Research. Volume 16. 1988. This is a ligninase coding sequence taken from ???Nucleotide Sequence of a ligninase gene from Phanerochaete Chrsosporium.??? Smith et al. Nucleic Acids Research. Volume 16. 1988. Ligninases are commonly used by fungi to degrade wood into materials useful for cell growth. false false _848_ 0 13358 9 Not in stock false We had to optimize the codons for expression in Escherichia coli. false Everett Frost annotation2176100 1 Protein Coding Sequence range2176100 1 1 1110 BBa_M36195 1 BBa_M36195 Bicistronic sequence containing ligninase and required enzyme manganese peroxidase 2012-06-02T11:00:00Z 2015-05-08T01:14:03Z 1. ???Nucleotide Sequence of a ligninase gene from Phanerochaete Chrsosporium.??? Smith et al. Nucleic Acids Research. Volume 16. 1988 2. Custom designed linker. See part BBa_M36191. 3. ???Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.??? Salame et al. Microbiology and Biotechnology. 2010. GenBank: FJ594281.1 The part contains a ligninase coding sequence attached to a manganese sequence through the use of a custom designed linker sequence. All the source information can be found under their part descriptions: 1. http://partsregistry.org/Part:BBa_M36191 2. http://partsregistry.org/Part:BBa_M36192 3. http://partsregistry.org/Part:BBa_M36187 false false _848_ 0 13358 9 Not in stock false The linker can be difficult to synthesize. false Everett Frost component2176097 1 BBa_M36191 component2176098 1 BBa_M36192 component2176099 1 BBa_M36187 annotation2176099 1 BBa_M36187 range2176099 1 1231 2304 annotation2176097 1 BBa_M36191 range2176097 1 1 1110 annotation2176098 1 BBa_M36192 range2176098 1 1119 1224 BBa_M36187 1 BBa_M36187 Manganese Peroxidase 2012-05-07T11:00:00Z 2015-05-08T01:14:03Z ???Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.??? Salame et al. Microbiology and Biotechnology. 2010. GenBank: FJ594281.1 This DNA sequence codes for a Manganese Peroxidase protein. This protein catalyzes the reaction of Mn (2+) to Mn (3+) in the presence of water and hydronium ions. false false _848_ 0 13358 9 Not in stock false None false Everett Frost annotation2176102 1 Manganese Peroxidase Coding Sequence range2176102 1 1 1074 BBa_M36191_sequence 1 gcctttaaacagctgttcgctgcgatctctcttgctctgcttctgagcgccgcgaacgcagctgctgttattcagaaacgtgctacttgcagcaacggcaaatatgttggagatgcttcatgctgtgcgtggtttgacgtgctggacgatatccagaacctctttcacggcggtcagtgcggtgcggaagcgcacgaatctattcgcctggtgtttcacgactctatcgcaatttctccagcaatggaggctcagggtaagttcggtggaggcggcgcggatggttctatcatgatctttgatgatattgaaactgcatttcacccgaatatcggtctcgacgaaatcgtgaaactgcagaaaccgttcgttcagaaacatggtgttaccccgggtgacttcatcgcgtttgcaggcgcggtggcactgagcaactgtccaggtgcaccgcagatgaacttcttcaccggccgcgcgccggcaactcaaccggcgccggatggactggttccggaaccgttccatactgtggatcagatcatcaaccgtgttaacgatgcaggcgaatttgacgaactggagctggtttggatgctgtctgctcactctgtggcggccgttaacgacgtaaacccgactgtccagggactgcctttcgactccacccctggcatcttcgattcccaattcttcgttgaaacccagctgcgtggtaccgcattcccgggtagcggtggcaaccagggcgaagttgagtctccgctgccgggtgaaattcgtattcagagcgatcacacgatcgcacgtgattctcgtacagcttgcgaatggcagagcttcgtcaacaaccagagcaagctggttgacgacttccagttcatctttctggccctgacccagctgggccaggatccgaacgcaatgaccgactgcagtgacgttatcccgcagagcaagccgatcccgggcaacctgccgttttccttcttccctgcgggtaaaaccatcaaagacgtcgaacaggcttgcgcagaaaccccatttccgactctgaccaccctgccgggcccagaaacgtccgtacagcgtatcccgccgccgccgggcgct BBa_M36187_sequence 1 atggccttcaagcacttctcgtcccttgtcctcctgactttggcatctcaagccgtccgaggcgctgtcatgaagcgtgctacttgcgctgacggacgtactactgcgaacgccgcctgctgcgtcctattccccattctggatgacatccaagaggccctctttgatggtgccgagtgtggtgaagaggttcacgagtcccttcgactcactttccatgacgctattggtttctcgccaaccaaaggtgggggaggtgccgacggctctatcgtaactttcgacgaaatcgaaactgctttccacgccaacggtggaatcgacgacatagtcgatgcgcagaagccatttattgctcgccacaacatctccgccggtgacttcattcaatttgctggcgctgttggtgtcagtaactgcccaggagctccaagactcaacttcttgcttggtcgcccgccagctacagcagcttcgcccaatggtttgatccctgaaccattcgacaccgtcaccgatatccttgcccgtatgggtgacgctggcttcagcccggaagaggtcgttgcccttctggcctcacactccgttgctgccgcagaccacgtcgatgacaccattccaggaacacccttcgactccactccaggagaatttgactctcaattcttcatcgaaacccaactccgtggcactgctttcccaggtgttggcggtaaccaaggagaggtcgaatcgcctcttgctggagaaatccgtatccaatcagatcacgatcttgcccgtgattctcgcactgcttgcgagtggcaatccttcgttaacaaccaggccaagcttcaatccgctttcaaggctgctatggacaagttggctacactcggtcaagatcgctcaaagctcattgactgctctgatgttatccctgtccccaagccattgcagagcaaggcccatctccctgctggcctgacgatgaacaacatcgagcaagcgtgtgcttctacacccttcccagctctcactgccgaccctggcccagtcactactgtcccccctgtccccccttcataa BBa_M36192_sequence 1 taataaggtaagtaattaggtaacaataacgttgtgctaagtaagcgtaaataaaatgttctaaaataacgttaaagtgtaagtaaggaagtaataaaatatttaa BBa_M36195_sequence 1 gcctttaaacagctgttcgctgcgatctctcttgctctgcttctgagcgccgcgaacgcagctgctgttattcagaaacgtgctacttgcagcaacggcaaatatgttggagatgcttcatgctgtgcgtggtttgacgtgctggacgatatccagaacctctttcacggcggtcagtgcggtgcggaagcgcacgaatctattcgcctggtgtttcacgactctatcgcaatttctccagcaatggaggctcagggtaagttcggtggaggcggcgcggatggttctatcatgatctttgatgatattgaaactgcatttcacccgaatatcggtctcgacgaaatcgtgaaactgcagaaaccgttcgttcagaaacatggtgttaccccgggtgacttcatcgcgtttgcaggcgcggtggcactgagcaactgtccaggtgcaccgcagatgaacttcttcaccggccgcgcgccggcaactcaaccggcgccggatggactggttccggaaccgttccatactgtggatcagatcatcaaccgtgttaacgatgcaggcgaatttgacgaactggagctggtttggatgctgtctgctcactctgtggcggccgttaacgacgtaaacccgactgtccagggactgcctttcgactccacccctggcatcttcgattcccaattcttcgttgaaacccagctgcgtggtaccgcattcccgggtagcggtggcaaccagggcgaagttgagtctccgctgccgggtgaaattcgtattcagagcgatcacacgatcgcacgtgattctcgtacagcttgcgaatggcagagcttcgtcaacaaccagagcaagctggttgacgacttccagttcatctttctggccctgacccagctgggccaggatccgaacgcaatgaccgactgcagtgacgttatcccgcagagcaagccgatcccgggcaacctgccgttttccttcttccctgcgggtaaaaccatcaaagacgtcgaacaggcttgcgcagaaaccccatttccgactctgaccaccctgccgggcccagaaacgtccgtacagcgtatcccgccgccgccgggcgcttactagagtaataaggtaagtaattaggtaacaataacgttgtgctaagtaagcgtaaataaaatgttctaaaataacgttaaagtgtaagtaaggaagtaataaaatatttaatactagatggccttcaagcacttctcgtcccttgtcctcctgactttggcatctcaagccgtccgaggcgctgtcatgaagcgtgctacttgcgctgacggacgtactactgcgaacgccgcctgctgcgtcctattccccattctggatgacatccaagaggccctctttgatggtgccgagtgtggtgaagaggttcacgagtcccttcgactcactttccatgacgctattggtttctcgccaaccaaaggtgggggaggtgccgacggctctatcgtaactttcgacgaaatcgaaactgctttccacgccaacggtggaatcgacgacatagtcgatgcgcagaagccatttattgctcgccacaacatctccgccggtgacttcattcaatttgctggcgctgttggtgtcagtaactgcccaggagctccaagactcaacttcttgcttggtcgcccgccagctacagcagcttcgcccaatggtttgatccctgaaccattcgacaccgtcaccgatatccttgcccgtatgggtgacgctggcttcagcccggaagaggtcgttgcccttctggcctcacactccgttgctgccgcagaccacgtcgatgacaccattccaggaacacccttcgactccactccaggagaatttgactctcaattcttcatcgaaacccaactccgtggcactgctttcccaggtgttggcggtaaccaaggagaggtcgaatcgcctcttgctggagaaatccgtatccaatcagatcacgatcttgcccgtgattctcgcactgcttgcgagtggcaatccttcgttaacaaccaggccaagcttcaatccgctttcaaggctgctatggacaagttggctacactcggtcaagatcgctcaaagctcattgactgctctgatgttatccctgtccccaagccattgcagagcaaggcccatctccctgctggcctgacgatgaacaacatcgagcaagcgtgtgcttctacacccttcccagctctcactgccgaccctggcccagtcactactgtcccccctgtccccccttcataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z