BBa_M36191
1
BBa_M36191
Ligninase Coding Sequence
2012-06-02T11:00:00Z
2015-05-08T01:14:03Z
???Nucleotide Sequence of a ligninase gene from Phanerochaete Chrsosporium.??? Smith et al. Nucleic Acids Research. Volume 16. 1988.
This is a ligninase coding sequence taken from ???Nucleotide Sequence of a ligninase gene from Phanerochaete Chrsosporium.??? Smith et al. Nucleic Acids Research. Volume 16. 1988. Ligninases are commonly used by fungi to degrade wood into materials useful for cell growth.
false
false
_848_
0
13358
9
Not in stock
false
We had to optimize the codons for expression in Escherichia coli.
false
Everett Frost
annotation2176100
1
Protein Coding Sequence
range2176100
1
1
1110
BBa_M36195
1
BBa_M36195
Bicistronic sequence containing ligninase and required enzyme manganese peroxidase
2012-06-02T11:00:00Z
2015-05-08T01:14:03Z
1. ???Nucleotide Sequence of a ligninase gene from Phanerochaete Chrsosporium.??? Smith et al. Nucleic Acids Research. Volume 16. 1988 2. Custom designed linker. See part BBa_M36191. 3. ???Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.??? Salame et al. Microbiology and Biotechnology. 2010. GenBank: FJ594281.1
The part contains a ligninase coding sequence attached to a manganese sequence through the use of a custom designed linker sequence. All the source information can be found under their part descriptions: 1. http://partsregistry.org/Part:BBa_M36191 2. http://partsregistry.org/Part:BBa_M36192 3. http://partsregistry.org/Part:BBa_M36187
false
false
_848_
0
13358
9
Not in stock
false
The linker can be difficult to synthesize.
false
Everett Frost
component2176098
1
BBa_M36192
component2176097
1
BBa_M36191
component2176099
1
BBa_M36187
annotation2176098
1
BBa_M36192
range2176098
1
1119
1224
annotation2176097
1
BBa_M36191
range2176097
1
1
1110
annotation2176099
1
BBa_M36187
range2176099
1
1231
2304
BBa_M36192
1
BBa_M36192
Bicistronic Linker Sequence
2012-06-02T11:00:00Z
2015-05-08T01:14:03Z
Aaron Thayer and Everett Frost custom designed this part.
This is a custom designed linker sequence designed to fit between two protein coding domains in a bicistronic system. The linker contains multiple frameshifted TAA stop codons and is long enough to prevent ribosome interference.
false
false
_848_
0
13358
9
Not in stock
false
Synthesis was difficult due to the number of TAA repeats.
false
Everett Frost
annotation2176101
1
Framshifted TAAs
range2176101
1
1
101
BBa_M36187
1
BBa_M36187
Manganese Peroxidase
2012-05-07T11:00:00Z
2015-05-08T01:14:03Z
???Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.??? Salame et al. Microbiology and Biotechnology. 2010. GenBank: FJ594281.1
This DNA sequence codes for a Manganese Peroxidase protein. This protein catalyzes the reaction of Mn (2+) to Mn (3+) in the presence of water and hydronium ions.
false
false
_848_
0
13358
9
Not in stock
false
None
false
Everett Frost
annotation2176102
1
Manganese Peroxidase Coding Sequence
range2176102
1
1
1074
BBa_M36191_sequence
1
gcctttaaacagctgttcgctgcgatctctcttgctctgcttctgagcgccgcgaacgcagctgctgttattcagaaacgtgctacttgcagcaacggcaaatatgttggagatgcttcatgctgtgcgtggtttgacgtgctggacgatatccagaacctctttcacggcggtcagtgcggtgcggaagcgcacgaatctattcgcctggtgtttcacgactctatcgcaatttctccagcaatggaggctcagggtaagttcggtggaggcggcgcggatggttctatcatgatctttgatgatattgaaactgcatttcacccgaatatcggtctcgacgaaatcgtgaaactgcagaaaccgttcgttcagaaacatggtgttaccccgggtgacttcatcgcgtttgcaggcgcggtggcactgagcaactgtccaggtgcaccgcagatgaacttcttcaccggccgcgcgccggcaactcaaccggcgccggatggactggttccggaaccgttccatactgtggatcagatcatcaaccgtgttaacgatgcaggcgaatttgacgaactggagctggtttggatgctgtctgctcactctgtggcggccgttaacgacgtaaacccgactgtccagggactgcctttcgactccacccctggcatcttcgattcccaattcttcgttgaaacccagctgcgtggtaccgcattcccgggtagcggtggcaaccagggcgaagttgagtctccgctgccgggtgaaattcgtattcagagcgatcacacgatcgcacgtgattctcgtacagcttgcgaatggcagagcttcgtcaacaaccagagcaagctggttgacgacttccagttcatctttctggccctgacccagctgggccaggatccgaacgcaatgaccgactgcagtgacgttatcccgcagagcaagccgatcccgggcaacctgccgttttccttcttccctgcgggtaaaaccatcaaagacgtcgaacaggcttgcgcagaaaccccatttccgactctgaccaccctgccgggcccagaaacgtccgtacagcgtatcccgccgccgccgggcgct
BBa_M36187_sequence
1
atggccttcaagcacttctcgtcccttgtcctcctgactttggcatctcaagccgtccgaggcgctgtcatgaagcgtgctacttgcgctgacggacgtactactgcgaacgccgcctgctgcgtcctattccccattctggatgacatccaagaggccctctttgatggtgccgagtgtggtgaagaggttcacgagtcccttcgactcactttccatgacgctattggtttctcgccaaccaaaggtgggggaggtgccgacggctctatcgtaactttcgacgaaatcgaaactgctttccacgccaacggtggaatcgacgacatagtcgatgcgcagaagccatttattgctcgccacaacatctccgccggtgacttcattcaatttgctggcgctgttggtgtcagtaactgcccaggagctccaagactcaacttcttgcttggtcgcccgccagctacagcagcttcgcccaatggtttgatccctgaaccattcgacaccgtcaccgatatccttgcccgtatgggtgacgctggcttcagcccggaagaggtcgttgcccttctggcctcacactccgttgctgccgcagaccacgtcgatgacaccattccaggaacacccttcgactccactccaggagaatttgactctcaattcttcatcgaaacccaactccgtggcactgctttcccaggtgttggcggtaaccaaggagaggtcgaatcgcctcttgctggagaaatccgtatccaatcagatcacgatcttgcccgtgattctcgcactgcttgcgagtggcaatccttcgttaacaaccaggccaagcttcaatccgctttcaaggctgctatggacaagttggctacactcggtcaagatcgctcaaagctcattgactgctctgatgttatccctgtccccaagccattgcagagcaaggcccatctccctgctggcctgacgatgaacaacatcgagcaagcgtgtgcttctacacccttcccagctctcactgccgaccctggcccagtcactactgtcccccctgtccccccttcataa
BBa_M36192_sequence
1
taataaggtaagtaattaggtaacaataacgttgtgctaagtaagcgtaaataaaatgttctaaaataacgttaaagtgtaagtaaggaagtaataaaatatttaa
BBa_M36195_sequence
1
gcctttaaacagctgttcgctgcgatctctcttgctctgcttctgagcgccgcgaacgcagctgctgttattcagaaacgtgctacttgcagcaacggcaaatatgttggagatgcttcatgctgtgcgtggtttgacgtgctggacgatatccagaacctctttcacggcggtcagtgcggtgcggaagcgcacgaatctattcgcctggtgtttcacgactctatcgcaatttctccagcaatggaggctcagggtaagttcggtggaggcggcgcggatggttctatcatgatctttgatgatattgaaactgcatttcacccgaatatcggtctcgacgaaatcgtgaaactgcagaaaccgttcgttcagaaacatggtgttaccccgggtgacttcatcgcgtttgcaggcgcggtggcactgagcaactgtccaggtgcaccgcagatgaacttcttcaccggccgcgcgccggcaactcaaccggcgccggatggactggttccggaaccgttccatactgtggatcagatcatcaaccgtgttaacgatgcaggcgaatttgacgaactggagctggtttggatgctgtctgctcactctgtggcggccgttaacgacgtaaacccgactgtccagggactgcctttcgactccacccctggcatcttcgattcccaattcttcgttgaaacccagctgcgtggtaccgcattcccgggtagcggtggcaaccagggcgaagttgagtctccgctgccgggtgaaattcgtattcagagcgatcacacgatcgcacgtgattctcgtacagcttgcgaatggcagagcttcgtcaacaaccagagcaagctggttgacgacttccagttcatctttctggccctgacccagctgggccaggatccgaacgcaatgaccgactgcagtgacgttatcccgcagagcaagccgatcccgggcaacctgccgttttccttcttccctgcgggtaaaaccatcaaagacgtcgaacaggcttgcgcagaaaccccatttccgactctgaccaccctgccgggcccagaaacgtccgtacagcgtatcccgccgccgccgggcgcttactagagtaataaggtaagtaattaggtaacaataacgttgtgctaagtaagcgtaaataaaatgttctaaaataacgttaaagtgtaagtaaggaagtaataaaatatttaatactagatggccttcaagcacttctcgtcccttgtcctcctgactttggcatctcaagccgtccgaggcgctgtcatgaagcgtgctacttgcgctgacggacgtactactgcgaacgccgcctgctgcgtcctattccccattctggatgacatccaagaggccctctttgatggtgccgagtgtggtgaagaggttcacgagtcccttcgactcactttccatgacgctattggtttctcgccaaccaaaggtgggggaggtgccgacggctctatcgtaactttcgacgaaatcgaaactgctttccacgccaacggtggaatcgacgacatagtcgatgcgcagaagccatttattgctcgccacaacatctccgccggtgacttcattcaatttgctggcgctgttggtgtcagtaactgcccaggagctccaagactcaacttcttgcttggtcgcccgccagctacagcagcttcgcccaatggtttgatccctgaaccattcgacaccgtcaccgatatccttgcccgtatgggtgacgctggcttcagcccggaagaggtcgttgcccttctggcctcacactccgttgctgccgcagaccacgtcgatgacaccattccaggaacacccttcgactccactccaggagaatttgactctcaattcttcatcgaaacccaactccgtggcactgctttcccaggtgttggcggtaaccaaggagaggtcgaatcgcctcttgctggagaaatccgtatccaatcagatcacgatcttgcccgtgattctcgcactgcttgcgagtggcaatccttcgttaacaaccaggccaagcttcaatccgctttcaaggctgctatggacaagttggctacactcggtcaagatcgctcaaagctcattgactgctctgatgttatccctgtccccaagccattgcagagcaaggcccatctccctgctggcctgacgatgaacaacatcgagcaagcgtgtgcttctacacccttcccagctctcactgccgaccctggcccagtcactactgtcccccctgtccccccttcataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z