LOCUS       BBa_M36196              2369 bp    DNA     linear       27-Dec-2024
DEFINITION  Bicistronic Lig/MnP -Frost/Thayer
ACCESSION   BBa_M36196
VERSION     BBa_M36196.1  
FEATURES             Location/Qualifiers
     CDS             1..1113
                     /label=BBa_M36188
     misc_feature    1122..1222
                     /label=Framshifted TAAs
     misc_feature    1122..1227
                     /label=BBa_M36192
     misc_feature    1240..1243
                     /label=conserved
     RBS             1236..1247
                     /label=BBa_B0034
     misc_feature    1254..2327
                     /label=Manganese Peroxidase Coding Sequence
     misc_feature    1254..2327
                     /label=BBa_M36187
     misc_feature    2336..2369
                     /label=B1002
     stem_loop       2345..2360
                     /label=stem loop
     polyA_site      2360..2366
                     /label=Poly A tail
     polyA_site      2339..2344
                     /label=Poly A tail
     terminator      2336..2369
                     /label=BBa_B1002
ORIGIN
        1 atggccttca aacaactctt cgctgcaatt tccctcgctc tgctcttgtc cgctgctaat
       61 gctgccgcag ttatccagaa acgtgctacc tgtagcaacg gtaagtacgt tggtgatgca
      121 agctgctgcg cctggtttga tgtcctggat gatattcaga atctgtttca cggtggtcaa
      181 tgcggtgcgg aagcacacga gagcatccgc ctggtgttcc acgacagcat tgcaatcagc
      241 cctgcgatgg aagcgcaggg taagttcggc ggtggcggtg ccgatggtag catcatgatc
      301 ttcgacgaca tcgaaaccgc cttccacccg aatatcggtc tggacgagat tgtgaaactg
      361 caaaaaccgt ttgtgcagaa gcatggtgta accccaggtg acttcatcgc gtttgcaggt
      421 gcggtcgcgc tgagcaactg cccgggtgcg ccgcagatga atttctttac gggccgtgct
      481 ccggcgacgc agccagcacc agacggtttg gtgccggaac cgtttcatac cgttgaccag
      541 attatcaatc gtgtcaacga tgcaggcgag ttcgacgagc tggagttggt ctggatgctg
      601 agcgcgcaca gcgtcgcggc agtgaatgac gttaacccga ctgtccaagg cttgccgttt
      661 gatagcacgc cgggtatttt cgacagccaa ttcttcgttg aaacccaact gcgcggcacg
      721 gccttccctg gtagcggtgg caatcagggt gaagtggaaa gcccgctgcc gggtgagatc
      781 cgcattcaga gcgaccatac gattgcgcgc gattcccgta cggcgtgcga gtggcagtct
      841 ttcgttaaca atcagtcgaa gctggtggat gactttcagt tcatctttct ggcgctgacc
      901 cagctgggcc aagatccgaa cgcaatgacg gactgtagcg acgtgatccc gcagagcaag
      961 ccgatcccgg gcaatctgcc tttttctttc ttccctgcgg gtaaaaccat taaagacgtt
     1021 gagcaggcct gtgccgaaac cccgttccca accctgacga cgctgcctgg cccggaaacg
     1081 tcggtgcaac gtattccgcc accaccgggt gcctactaga gtaataaggt aagtaattag
     1141 gtaacaataa cgttgtgcta agtaagcgta aataaaatgt tctaaaataa cgttaaagtg
     1201 taagtaagga agtaataaaa tatttaatac tagagaaaga ggagaaatac tagatggcct
     1261 tcaagcactt ctcgtccctt gtcctcctga ctttggcatc tcaagccgtc cgaggcgctg
     1321 tcatgaagcg tgctacttgc gctgacggac gtactactgc gaacgccgcc tgctgcgtcc
     1381 tattccccat tctggatgac atccaagagg ccctctttga tggtgccgag tgtggtgaag
     1441 aggttcacga gtcccttcga ctcactttcc atgacgctat tggtttctcg ccaaccaaag
     1501 gtgggggagg tgccgacggc tctatcgtaa ctttcgacga aatcgaaact gctttccacg
     1561 ccaacggtgg aatcgacgac atagtcgatg cgcagaagcc atttattgct cgccacaaca
     1621 tctccgccgg tgacttcatt caatttgctg gcgctgttgg tgtcagtaac tgcccaggag
     1681 ctccaagact caacttcttg cttggtcgcc cgccagctac agcagcttcg cccaatggtt
     1741 tgatccctga accattcgac accgtcaccg atatccttgc ccgtatgggt gacgctggct
     1801 tcagcccgga agaggtcgtt gcccttctgg cctcacactc cgttgctgcc gcagaccacg
     1861 tcgatgacac cattccagga acacccttcg actccactcc aggagaattt gactctcaat
     1921 tcttcatcga aacccaactc cgtggcactg ctttcccagg tgttggcggt aaccaaggag
     1981 aggtcgaatc gcctcttgct ggagaaatcc gtatccaatc agatcacgat cttgcccgtg
     2041 attctcgcac tgcttgcgag tggcaatcct tcgttaacaa ccaggccaag cttcaatccg
     2101 ctttcaaggc tgctatggac aagttggcta cactcggtca agatcgctca aagctcattg
     2161 actgctctga tgttatccct gtccccaagc cattgcagag caaggcccat ctccctgctg
     2221 gcctgacgat gaacaacatc gagcaagcgt gtgcttctac acccttccca gctctcactg
     2281 ccgaccctgg cccagtcact actgtccccc ctgtcccccc ttcataatac tagagcgcaa
     2341 aaaaccccgc ttcggcgggg ttttttcgc
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