BBa_B1002 1 BBa_B1002 Terminator (artificial, small, %T~=85%) 2006-08-29T11:00:00Z 2015-08-31T04:07:21Z antiquity Artifical terminator, estimated %T~=85 false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues. true Haiyao Huang annotation1898414 1 Poly A tail range1898414 1 25 31 annotation1898413 1 stem loop range1898413 1 10 25 annotation1898415 1 Poly A tail range1898415 1 4 9 annotation1898412 1 B1002 range1898412 1 1 34 BBa_M36187 1 BBa_M36187 Manganese Peroxidase 2012-05-07T11:00:00Z 2015-05-08T01:14:03Z ???Pleurotus ostreatus manganese-dependent peroxidase silencing impairs decolourization of Orange II.??? Salame et al. Microbiology and Biotechnology. 2010. GenBank: FJ594281.1 This DNA sequence codes for a Manganese Peroxidase protein. This protein catalyzes the reaction of Mn (2+) to Mn (3+) in the presence of water and hydronium ions. false false _848_ 0 13358 9 Not in stock false None false Everett Frost annotation2176102 1 Manganese Peroxidase Coding Sequence range2176102 1 1 1074 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M36196 1 BBa_M36196 Bicistronic Lig/MnP -Frost/Thayer 2012-06-02T11:00:00Z 2015-05-08T01:14:03Z http://partsregistry.org/Part:BBa_M36188, http://partsregistry.org/Part:BBa_M36192, http://partsregistry.org/Part:BBa_B0034, http://partsregistry.org/Part:BBa_M36187, http://partsregistry.org/Part:BBa_B1002 This is the entire bicistronic system that we used to create our genetic design. Ligninase is a protein used for lignin degradation by microbes and fungi. Manganese peroxidase is a necessary coenzyme for ligninase activity. false false _848_ 0 13358 9 Not in stock false This design is codon optimized for expression in escherichia coli. We had some difficulty with synthesis of the repeats in the linker sequence and repeats in the BBa_B0034 terminator. false Everett Frost component2176105 1 BBa_M36192 component2176109 1 BBa_M36187 component2176103 1 BBa_M36188 component2176107 1 BBa_B0034 component2176114 1 BBa_B1002 annotation2176103 1 BBa_M36188 range2176103 1 1 1113 annotation2176107 1 BBa_B0034 range2176107 1 1236 1247 annotation2176109 1 BBa_M36187 range2176109 1 1254 2327 annotation2176105 1 BBa_M36192 range2176105 1 1122 1227 annotation2176114 1 BBa_B1002 range2176114 1 2336 2369 BBa_M36192 1 BBa_M36192 Bicistronic Linker Sequence 2012-06-02T11:00:00Z 2015-05-08T01:14:03Z Aaron Thayer and Everett Frost custom designed this part. This is a custom designed linker sequence designed to fit between two protein coding domains in a bicistronic system. The linker contains multiple frameshifted TAA stop codons and is long enough to prevent ribosome interference. false false _848_ 0 13358 9 Not in stock false Synthesis was difficult due to the number of TAA repeats. false Everett Frost annotation2176101 1 Framshifted TAAs range2176101 1 1 101 BBa_M36188 1 BBa_M36188 Ligninase Coding Region 2012-05-02T11:00:00Z 2015-05-08T01:14:03Z MAFKQLFAAISLALLLSAANAAAVIQKRATCSNGKYVGDASCCAWFDVLDDIQNLFHGGQCGAEAHESIRLVFHDSIAISPAMEAQGKFGGGGADGSIMIFDDIETAFHPNIGLDEIVKLQKPFVQKHGVTPGDFIAFAGAVALSNCPGAPQMNFFTGRAPATQPAPDGLVPEPFHTVDQIINRVNDAGEFDELELVWMLSAHSVAAVNDVNPTVQGLPFDSTPGIFDSQFFVETQLRGTAFPGSGGNQGEVESPLPGEIRIQSDHTIARDSRTACEWQSFVNNQSKLVDDFQFIFLALTQLGQDPNAMTDCSDVIPQSKPIPGNLPFSFFPAGKTIKDVEQACAETPFPTLTTLPGPETSVQRIPPPPGA This coding region has been created from Cullen et al "Nucleotide sequence of a ligninase gene from Phanerochaete chrysosporium." The sequence was modified so that all introns were removed and potential looping eliminated. Additionally, the amino acids have been chosen such that the tRNA ratios for e. coli has been optimized. Minimizing loops was done using Gene Designer and checking for tRNA in e.coli was accomplished using Optimizer. The coding sequence is given seen here is not identical to the sequence given by Cullen et al. However, this sequence has been checked against the Blast database and been found to be highly homologous to multiple Ligninase Peroxidase Regions. GIven here is the Amino Acid Sequence. false false _848_ 0 13233 9 Not in stock false This enzyme works at pH 4.5 as found here: http://www.springerlink.com/content/7616k56005852420/fulltext.pdf?MUD=MP Sanroman et al. "Optimum stability conditions of pH and temperature for ligninaseand manganese-dependent peroxidase from Phanerochaete chrysosporium. Application to in vitro decolorization of Poly R-478 by MnP." World Journal of Microbiology and Biotechnology. 22: 607-612. 2006. false Aaron Thayer BBa_B1002_sequence 1 cgcaaaaaaccccgcttcggcggggttttttcgc BBa_B0034_sequence 1 aaagaggagaaa BBa_M36188_sequence 1 atggccttcaaacaactcttcgctgcaatttccctcgctctgctcttgtccgctgctaatgctgccgcagttatccagaaacgtgctacctgtagcaacggtaagtacgttggtgatgcaagctgctgcgcctggtttgatgtcctggatgatattcagaatctgtttcacggtggtcaatgcggtgcggaagcacacgagagcatccgcctggtgttccacgacagcattgcaatcagccctgcgatggaagcgcagggtaagttcggcggtggcggtgccgatggtagcatcatgatcttcgacgacatcgaaaccgccttccacccgaatatcggtctggacgagattgtgaaactgcaaaaaccgtttgtgcagaagcatggtgtaaccccaggtgacttcatcgcgtttgcaggtgcggtcgcgctgagcaactgcccgggtgcgccgcagatgaatttctttacgggccgtgctccggcgacgcagccagcaccagacggtttggtgccggaaccgtttcataccgttgaccagattatcaatcgtgtcaacgatgcaggcgagttcgacgagctggagttggtctggatgctgagcgcgcacagcgtcgcggcagtgaatgacgttaacccgactgtccaaggcttgccgtttgatagcacgccgggtattttcgacagccaattcttcgttgaaacccaactgcgcggcacggccttccctggtagcggtggcaatcagggtgaagtggaaagcccgctgccgggtgagatccgcattcagagcgaccatacgattgcgcgcgattcccgtacggcgtgcgagtggcagtctttcgttaacaatcagtcgaagctggtggatgactttcagttcatctttctggcgctgacccagctgggccaagatccgaacgcaatgacggactgtagcgacgtgatcccgcagagcaagccgatcccgggcaatctgcctttttctttcttccctgcgggtaaaaccattaaagacgttgagcaggcctgtgccgaaaccccgttcccaaccctgacgacgctgcctggcccggaaacgtcggtgcaacgtattccgccaccaccgggtgcc BBa_M36187_sequence 1 atggccttcaagcacttctcgtcccttgtcctcctgactttggcatctcaagccgtccgaggcgctgtcatgaagcgtgctacttgcgctgacggacgtactactgcgaacgccgcctgctgcgtcctattccccattctggatgacatccaagaggccctctttgatggtgccgagtgtggtgaagaggttcacgagtcccttcgactcactttccatgacgctattggtttctcgccaaccaaaggtgggggaggtgccgacggctctatcgtaactttcgacgaaatcgaaactgctttccacgccaacggtggaatcgacgacatagtcgatgcgcagaagccatttattgctcgccacaacatctccgccggtgacttcattcaatttgctggcgctgttggtgtcagtaactgcccaggagctccaagactcaacttcttgcttggtcgcccgccagctacagcagcttcgcccaatggtttgatccctgaaccattcgacaccgtcaccgatatccttgcccgtatgggtgacgctggcttcagcccggaagaggtcgttgcccttctggcctcacactccgttgctgccgcagaccacgtcgatgacaccattccaggaacacccttcgactccactccaggagaatttgactctcaattcttcatcgaaacccaactccgtggcactgctttcccaggtgttggcggtaaccaaggagaggtcgaatcgcctcttgctggagaaatccgtatccaatcagatcacgatcttgcccgtgattctcgcactgcttgcgagtggcaatccttcgttaacaaccaggccaagcttcaatccgctttcaaggctgctatggacaagttggctacactcggtcaagatcgctcaaagctcattgactgctctgatgttatccctgtccccaagccattgcagagcaaggcccatctccctgctggcctgacgatgaacaacatcgagcaagcgtgtgcttctacacccttcccagctctcactgccgaccctggcccagtcactactgtcccccctgtccccccttcataa BBa_M36196_sequence 1 atggccttcaaacaactcttcgctgcaatttccctcgctctgctcttgtccgctgctaatgctgccgcagttatccagaaacgtgctacctgtagcaacggtaagtacgttggtgatgcaagctgctgcgcctggtttgatgtcctggatgatattcagaatctgtttcacggtggtcaatgcggtgcggaagcacacgagagcatccgcctggtgttccacgacagcattgcaatcagccctgcgatggaagcgcagggtaagttcggcggtggcggtgccgatggtagcatcatgatcttcgacgacatcgaaaccgccttccacccgaatatcggtctggacgagattgtgaaactgcaaaaaccgtttgtgcagaagcatggtgtaaccccaggtgacttcatcgcgtttgcaggtgcggtcgcgctgagcaactgcccgggtgcgccgcagatgaatttctttacgggccgtgctccggcgacgcagccagcaccagacggtttggtgccggaaccgtttcataccgttgaccagattatcaatcgtgtcaacgatgcaggcgagttcgacgagctggagttggtctggatgctgagcgcgcacagcgtcgcggcagtgaatgacgttaacccgactgtccaaggcttgccgtttgatagcacgccgggtattttcgacagccaattcttcgttgaaacccaactgcgcggcacggccttccctggtagcggtggcaatcagggtgaagtggaaagcccgctgccgggtgagatccgcattcagagcgaccatacgattgcgcgcgattcccgtacggcgtgcgagtggcagtctttcgttaacaatcagtcgaagctggtggatgactttcagttcatctttctggcgctgacccagctgggccaagatccgaacgcaatgacggactgtagcgacgtgatcccgcagagcaagccgatcccgggcaatctgcctttttctttcttccctgcgggtaaaaccattaaagacgttgagcaggcctgtgccgaaaccccgttcccaaccctgacgacgctgcctggcccggaaacgtcggtgcaacgtattccgccaccaccgggtgcctactagagtaataaggtaagtaattaggtaacaataacgttgtgctaagtaagcgtaaataaaatgttctaaaataacgttaaagtgtaagtaaggaagtaataaaatatttaatactagagaaagaggagaaatactagatggccttcaagcacttctcgtcccttgtcctcctgactttggcatctcaagccgtccgaggcgctgtcatgaagcgtgctacttgcgctgacggacgtactactgcgaacgccgcctgctgcgtcctattccccattctggatgacatccaagaggccctctttgatggtgccgagtgtggtgaagaggttcacgagtcccttcgactcactttccatgacgctattggtttctcgccaaccaaaggtgggggaggtgccgacggctctatcgtaactttcgacgaaatcgaaactgctttccacgccaacggtggaatcgacgacatagtcgatgcgcagaagccatttattgctcgccacaacatctccgccggtgacttcattcaatttgctggcgctgttggtgtcagtaactgcccaggagctccaagactcaacttcttgcttggtcgcccgccagctacagcagcttcgcccaatggtttgatccctgaaccattcgacaccgtcaccgatatccttgcccgtatgggtgacgctggcttcagcccggaagaggtcgttgcccttctggcctcacactccgttgctgccgcagaccacgtcgatgacaccattccaggaacacccttcgactccactccaggagaatttgactctcaattcttcatcgaaacccaactccgtggcactgctttcccaggtgttggcggtaaccaaggagaggtcgaatcgcctcttgctggagaaatccgtatccaatcagatcacgatcttgcccgtgattctcgcactgcttgcgagtggcaatccttcgttaacaaccaggccaagcttcaatccgctttcaaggctgctatggacaagttggctacactcggtcaagatcgctcaaagctcattgactgctctgatgttatccctgtccccaagccattgcagagcaaggcccatctccctgctggcctgacgatgaacaacatcgagcaagcgtgtgcttctacacccttcccagctctcactgccgaccctggcccagtcactactgtcccccctgtccccccttcataatactagagcgcaaaaaaccccgcttcggcggggttttttcgc BBa_M36192_sequence 1 taataaggtaagtaattaggtaacaataacgttgtgctaagtaagcgtaaataaaatgttctaaaataacgttaaagtgtaagtaaggaagtaataaaatatttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z