BBa_M36201 1 RhmTrm Rhamnex 67K Terminator and pUC ori 2011-12-04T12:00:00Z 2015-05-08T01:14:03Z This part was taken from Gene Designer's Rhamnex 67K (pUC ori Kana) vector. The Rhamnex 67K vector is one of their "Xbrane Vectors" This terminator and pUC ori are a combination of a terminator sequence and an origin or replication. The terminator is a class 1 terminator that ends transcription of the operon. The pUC origin of replication is used for DNA replication and dictates the number of copies of the plasmid that the host organism will make (in this case, is approximately 250 copies per cell) false false _848_ 0 11060 9 Not in stock false n/a false Simon Zheng, Kevin Aliado, Steven Lee annotation2166389 1 terminator and pUC ori range2166389 1 1 1178 BBa_M36200 1 ADH ADH; converts ethanol to/from acetaldehyde; no STOP codon 2011-12-04T12:00:00Z 2015-05-08T01:14:03Z This construct originally codes for Alcohol Dehydrogenase in Acetobacter pasteurianus. Amino acid sequence for Acetobacter pasteurianus can be found here: http://www.ncbi.nlm.nih.gov/nuccore/NC_013211.1?from=15149 ADH = Alcohol Dehydrogenase. This construct codes for both ADH1, which converts acetaldehyde to ethanol, and ADH2, which converts ethanol to acetaldehyde. ADH1 is activated by the presence of acetaldehyde, while ADH2 is activated by the presence of ethanol. This construct is regulated by any PoPS-regulating sensor. false false _848_ 0 11059 9 Not in stock false The construct is codon optimized for Escherichia coli. false Kevin Aliado, Steven Lee, Simon Zheng annotation2166408 1 START range2166408 1 1 3 annotation2166410 1 ADH range2166410 1 4 1614 BBa_M36202 1 6HisTag 6-Histidine Tag 2011-12-05T12:00:00Z 2015-05-08T01:14:03Z N/A This codes for a 6-histidine tag that produces an amino acid chain that can be used with common Western Blots and stains. false false _848_ 0 11067 9 Not in stock false To avoid long repeats, we used alternating His codons for the last 3 histidines. false Steven Lee BBa_M36204 1 BD14 Bicistronic RBS (BD14) 2011-12-06T12:00:00Z 2015-05-08T01:14:03Z Andrew Endy's design. More information found at BIOFAB.org. This is a bicistronic RBS, consisting of a constitutive RBS, followed by a leader sequence that includes the second RBS. The first constitutive RBS uses ribosomes to physically occupy the second RBS, preventing the second RBS from binding to genes of interest downstream. This ensures a more consistent, stable expression level of your gene of interest. This bicistronic RBS was designed by Andrew Endy. More information can be found at BIOFAB.org. false false _848_ 0 11067 9 Not in stock false N/A false Steven Lee BBa_M36203 1 ADHAc ADH Actuator; input is PoPS and output is ADH enzyme 2011-12-06T12:00:00Z 2015-05-08T01:14:03Z The ADH sequence in the actuator originally codes for Alcohol Dehydrogenase in Acetobacter pasteurianus. Amino acid sequence for Acetobacter pasteurianus can be found here: http://www.ncbi.nlm.nih.gov/nuccore/NC_013211.1?from=15149 This actuator receives an input of polymerases per second from a standard sensor and produces ADH1 (Alcohol Dehydrogenase 1) and ADH2 linked to a 6-histidine tag for any necessary for quantitative assays. This actuator utilizes a bicistronic RBS to prevent binding of the RBS with any other parts of the coding sequence and to maintain consistency in the transcription rate. This actuator consists of ADH1, which converts acetaldehyde to ethanol, and ADH2, which converts ethanol to acetaldehyde. ADH1 is activated by the presence of acetaldehyde, while ADH2 is activated by the presence of ethanol. false false _848_ 0 11059 9 Not in stock true (1)Codon optimized for Escherichia coli. (2)6-his tag added. false Kevin Aliado, Steven Lee, Simon Zheng component2166417 1 BBa_M36202 component2166413 1 BBa_M36204 component2166416 1 BBa_M36200 component2166419 1 BBa_M36201 annotation2166419 1 BBa_M36201 range2166419 1 1718 2895 annotation2166413 1 BBa_M36204 range2166413 1 1 85 annotation2166417 1 BBa_M36202 range2166417 1 1700 1717 annotation2166416 1 BBa_M36200 range2166416 1 86 1699 BBa_M36200_sequence 1 atgtccggccagaacgatttccctaccgagttcgacttcatcatcgtgggtgcaggtgccgcaggttgcgttcttgccaaccgtctgtccgctcgcagcaacttacgtgtggccctgctggaagctggtcaagcagataacacgccgcgtattcacgttccagcaggtaccatttccctgtacaaaagccgtaaatacacataccagtattactccaccccgcagaagtacctgaacaaccgtcgtatccacgtcccgcgcggtcgtatgctgggtgggtcttcatccatgaacagcatgatctacattcgtggtgcgcgcagcgattacgatgactgggaagctatgggttgtaccggctggggctacgatgctgtactgaaatatttcatgcgcgaggaaaacaatcacctgcatcaagacccgcacttccacggcacgggcggcgaactggttgttgaccagccacgtgacccgctgggtgtctctcgtctgtttatcaaagcggcagaagaagtgggtctcaaagagaacactgacttcaacggtgcgaaactggacggcgtaggtatttatgacgtcactcagaaagacggcaaacgtctgtctgcgtaccgtgccttcgtcgcaccggttcgttcccgcccgaacctgcacgttgttaccggttgtaaagttgtaagcttagtcaccgatggcaaagaagttcagggtgtaaccatcgaacgtaacggccaattccatgtgctgcgtgcgcgtcgtgaaaccatcctgtccgcgggtgcgatcgggtccccgcatctgctgatgtcctctggtatcggtaacgctcgtgagctgctggctgcgggtgtaccggtggttgcagatctcccggaagttggccgtaatctgcaggatcacgttgacggcctggtgaccatccgttccgattctgcatctaccttgggtttctccaccgcatccctgagctcagtggttccgagcccactgcagttcctgctgaaacgtaaaggttggctgaccaccaattacgttgaagcaggtggctttgcttctacccgctacgctaaagatgtgccggatatccagttccatttcgttccgggctaccgctctcaccgcggccgcttatttgaatggggtcacggcttcgccctgcacacctgcgtgcttcgtccgtactctcgtggctccatccgtctggcacgtgacggtagccgcaacccagatattgacttcaactttctgtctgatgaacgtgacacccacgttctgctggaaggtgtaaaactggcgcgtagtatcctgcgcgcgtctccgttcgatgccatccgtggcaaagaaatggctccgaccgcgaacatccagactgatgaccagctgattgagtacctgcgcgcatccgcctcgaccgttttccacccgagcggtacttgtcgcatgggcgctgacgacacgtctgtagttacaccggatctgaaagttcgcggtctgaaaggtctgcgtgtagccgatacctctattatgccgactctggtttccggtaacaccaacgcaccgaccatgatgatcggtgacaaagcatccgatatgatcctggcagatgcagtg BBa_M36201_sequence 1 ctcgaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag BBa_M36202_sequence 1 catcatcatcaccatcac BBa_M36203_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcggtggagggtttctaatgtccggccagaacgatttccctaccgagttcgacttcatcatcgtgggtgcaggtgccgcaggttgcgttcttgccaaccgtctgtccgctcgcagcaacttacgtgtggccctgctggaagctggtcaagcagataacacgccgcgtattcacgttccagcaggtaccatttccctgtacaaaagccgtaaatacacataccagtattactccaccccgcagaagtacctgaacaaccgtcgtatccacgtcccgcgcggtcgtatgctgggtgggtcttcatccatgaacagcatgatctacattcgtggtgcgcgcagcgattacgatgactgggaagctatgggttgtaccggctggggctacgatgctgtactgaaatatttcatgcgcgaggaaaacaatcacctgcatcaagacccgcacttccacggcacgggcggcgaactggttgttgaccagccacgtgacccgctgggtgtctctcgtctgtttatcaaagcggcagaagaagtgggtctcaaagagaacactgacttcaacggtgcgaaactggacggcgtaggtatttatgacgtcactcagaaagacggcaaacgtctgtctgcgtaccgtgccttcgtcgcaccggttcgttcccgcccgaacctgcacgttgttaccggttgtaaagttgtaagcttagtcaccgatggcaaagaagttcagggtgtaaccatcgaacgtaacggccaattccatgtgctgcgtgcgcgtcgtgaaaccatcctgtccgcgggtgcgatcgggtccccgcatctgctgatgtcctctggtatcggtaacgctcgtgagctgctggctgcgggtgtaccggtggttgcagatctcccggaagttggccgtaatctgcaggatcacgttgacggcctggtgaccatccgttccgattctgcatctaccttgggtttctccaccgcatccctgagctcagtggttccgagcccactgcagttcctgctgaaacgtaaaggttggctgaccaccaattacgttgaagcaggtggctttgcttctacccgctacgctaaagatgtgccggatatccagttccatttcgttccgggctaccgctctcaccgcggccgcttatttgaatggggtcacggcttcgccctgcacacctgcgtgcttcgtccgtactctcgtggctccatccgtctggcacgtgacggtagccgcaacccagatattgacttcaactttctgtctgatgaacgtgacacccacgttctgctggaaggtgtaaaactggcgcgtagtatcctgcgcgcgtctccgttcgatgccatccgtggcaaagaaatggctccgaccgcgaacatccagactgatgaccagctgattgagtacctgcgcgcatccgcctcgaccgttttccacccgagcggtacttgtcgcatgggcgctgacgacacgtctgtagttacaccggatctgaaagttcgcggtctgaaaggtctgcgtgtagccgatacctctattatgccgactctggtttccggtaacaccaacgcaccgaccatgatgatcggtgacaaagcatccgatatgatcctggcagatgcagtgcatcatcatcaccatcacctcgaattcgtaatcatgtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacag BBa_M36204_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcggtggagggtttcta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z