LOCUS       BBa_M36210              1479 bp    DNA     linear       22-Nov-2024
DEFINITION  Nitric oxide sensing construct for use in Bacillus subtilis
ACCESSION   BBa_M36210
VERSION     BBa_M36210.1  
FEATURES             Location/Qualifiers
     promoter        1..186
                     /label=pNO
     promoter        1..186
                     /label=BBa_K256000
     misc_feature    280..282
                     /label=Start Codon
     RBS             195..282
                     /label=BBa_M36282
     misc_feature    291..1373
                     /label=BBa_K082004
     CDS             291..1373
                     /label=BBa_K082004
     misc_feature    1382..1415
                     /label=B1002
     stem_loop       1391..1406
                     /label=stem loop
     polyA_site      1406..1412
                     /label=Poly A tail
     polyA_site      1385..1390
                     /label=Poly A tail
     terminator      1382..1415
                     /label=BBa_B1002
     promoter        1440..1445
                     /label=-35
     promoter        1467..1472
                     /label=-10
     promoter        1424..1479
                     /label=BBa_K091110
ORIGIN
        1 agcaacctca atttattcag cgtgttctaa aaagatgtct tgctaattgc agattgcgtg
       61 ccagtttttt atctgattgt ttttactcta taaataaaat tatgcccatt aatgagtagt
      121 caaaatgact atcaatcaaa tggtcgatat gacaatatct atagtcaaat tgacagtgag
      181 gcaaagtact agaggggccc aagttcactt aaaaaggaga tcaacaatga aagcaatttt
      241 cgtactgaaa catcttaatc atgctaagga ggttttctaa tgtactagag gtgaaaccag
      301 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
      361 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaga agtggaagcg gcgatggcgg
      421 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
      481 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
      541 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
      601 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
      661 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
      721 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
      781 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
      841 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
      901 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
      961 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
     1021 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     1081 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     1141 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     1201 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     1261 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1321 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgatactaga
     1381 gcgcaaaaaa ccccgcttcg gcggggtttt ttcgctacta gagcgttgac accatcgaat
     1441 ggcgcaaaac ctttcgcggt atggcatgat agcgcccgg
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