BBa_K256000 1 pNO NO sensing promoter 2009-10-15T11:00:00Z 2015-05-08T01:11:40Z -- NO sensing promoter false false _359_ 0 4864 9 Not in stock false -- false iGEM09_NTU-Singapore annotation2055459 1 pNO range2055459 1 1 186 BBa_M36210 1 NO Sensor Nitric oxide sensing construct for use in Bacillus subtilis 2011-12-07T12:00:00Z 2015-05-08T01:14:03Z Source: Description of how NO promoter is regulated by lacI false false _848_ 0 11053 9 Not in stock false Add start ___ false Michele Dragoescu, Shaheen Jeeawoody, Lizzie Peiros component2166738 1 BBa_K082004 component2166743 1 BBa_B1002 component2166737 1 BBa_M36282 component2166746 1 BBa_K091110 component2166735 1 BBa_K256000 annotation2166737 1 BBa_M36282 range2166737 1 195 282 annotation2166735 1 BBa_K256000 range2166735 1 1 186 annotation2166743 1 BBa_B1002 range2166743 1 1382 1415 annotation2166746 1 BBa_K091110 range2166746 1 1424 1479 annotation2166738 1 BBa_K082004 range2166738 1 291 1373 BBa_M36282 1 BBa_M36282 5' Bicistronic UTR, includes ATG start codon 2011-11-29T12:00:00Z 2015-05-08T01:14:04Z BioFab: apFAB563 This sequence contains an RBS followed by the sequence TAATG. The parts number is BBa_M36000 (PartsRegistry). The first RBS sequence is strong, while the second one is weak. This stops the first polymerase with the stop codon TAA, but allows for a second polymerase to bind to the start codon ATG(BIOFAB). This sequence prevents a hairpin loop from being formed with another part of the sequence, which would decrease the amount of translation. The bicistronic sequence ends with a start codon ATG. false true _848_ 0 11068 9 Not in stock false no false Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso annotation2166677 1 Start Codon range2166677 1 86 88 BBa_K091110 1 BBa_K091110 LacI Promoter 2008-06-19T11:00:00Z 2015-05-08T01:08:37Z This promoter is a modified version of the LacI promoter This is a modified version of the LacI promoter that should bind LacI tighter than the original. false false _191_ 0 3080 9 It's complicated false no special considerations false Robert Cool annotation1964082 1 -10 range1964082 1 44 49 annotation1964081 1 -35 range1964081 1 17 22 BBa_B1002 1 BBa_B1002 Terminator (artificial, small, %T~=85%) 2006-08-29T11:00:00Z 2015-08-31T04:07:21Z antiquity Artifical terminator, estimated %T~=85 false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues. true Haiyao Huang annotation1898414 1 Poly A tail range1898414 1 25 31 annotation1898415 1 Poly A tail range1898415 1 4 9 annotation1898413 1 stem loop range1898413 1 10 25 annotation1898412 1 B1002 range1898412 1 1 34 BBa_K082004 1 BBa_K082004 LacI- wild type 2008-10-27T12:00:00Z 2015-05-08T01:08:35Z The The false false _228_ 0 1560 9 It's complicated false The false Yanting Xue , He Shen annotation1992777 1 BBa_K082004 range1992777 1 1 1083 BBa_B1002_sequence 1 cgcaaaaaaccccgcttcggcggggttttttcgc BBa_K256000_sequence 1 agcaacctcaatttattcagcgtgttctaaaaagatgtcttgctaattgcagattgcgtgccagttttttatctgattgtttttactctataaataaaattatgcccattaatgagtagtcaaaatgactatcaatcaaatggtcgatatgacaatatctatagtcaaattgacagtgaggcaaag BBa_M36282_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg BBa_M36210_sequence 1 agcaacctcaatttattcagcgtgttctaaaaagatgtcttgctaattgcagattgcgtgccagttttttatctgattgtttttactctataaataaaattatgcccattaatgagtagtcaaaatgactatcaatcaaatggtcgatatgacaatatctatagtcaaattgacagtgaggcaaagtactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagaggtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaagaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgatactagagcgcaaaaaaccccgcttcggcggggttttttcgctactagagcgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccgg BBa_K091110_sequence 1 cgttgacaccatcgaatggcgcaaaacctttcgcggtatggcatgatagcgcccgg BBa_K082004_sequence 1 gtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaagaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z