LOCUS BBa_M36222 2554 bp DNA linear 25-Nov-2024 DEFINITION ArsAction: Arsenite Removal Device (input: PoPs Signal, output: removal device) ACCESSION BBa_M36222 VERSION BBa_M36222.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..21 /label=Start codon ATG misc_feature 331..336 /label=Mutation:Restriction site BbsI removal misc_feature 493..498 /label=Mutation: Restriction site BsmbI removal CDS 19..1218 /label=BBa_M36220 RBS 1227..1240 /label=BBa_B0035 misc_feature 1247..1249 /label=start codon ATG misc_feature 1514..1520 /label=Restriction Site BsmBI misc_feature 1981..1987 /label=Restriction Site BsaI CDS 1247..2512 /label=BBa_M36221 misc_feature 2521..2554 /label=B1002 stem_loop 2530..2545 /label=stem loop polyA_site 2545..2551 /label=Poly A tail polyA_site 2524..2529 /label=Poly A tail terminator 2521..2554 /label=BBa_B1002 ORIGIN 1 aaagaggaga aatactagat gctgctcgat gtagaaccgc tggaaccgac cctctcgaac 61 attatcgagc agcgctctct taaatggatc ttcgttggcg gtaaaggtgg cgtaggtaag 121 accacctgct cttgctctct ggcagttcag ctgtccaaag gtcgcgagtc cgtgctcatc 181 atctcgaccg atccggccca caacatttcg gacgcgttcg atcagaaatt tagtaaagtt 241 ccaaccaagg ttaagggcta cgataacctc tttgctatgg agatcgaccc aagcctcggt 301 gtcgcggatg tgccggacga attcttcgag aacatgctgt ctatgggaaa aaaaatgatg 361 caggaggcaa tgtccgcgtt cccgggtatc gatgaagcca tgagctacgc ggaagtcatg 421 cgtctggtta aaggtatgaa cttcagcgtt gtggtattcg ataccgcgcc aaccggtcat 481 accctgctga atttcccgac cattgttgaa cgtggtctgg gtcgcctgat gcagatcaaa 541 aatcagatca gtccattcat ttcacagatg tgcaacatgc tgggtctggg tgacatgaac 601 gcggatcagc ttgcatccaa actggaggaa acccttcctg ttatccgtag cgttagcgaa 661 caatttaaag atccggaaca aacgactttt atttgcgttt gtatcgcgga attcctgtcc 721 ctgtacgaaa ccgaacgtct gatccaggaa ctggccaaat gtaaaattga cacccataac 781 atcatcgtaa atcagctggt gttccctgac ccggaaaaac cgtgcaaaat gtgtgaagcc 841 cgtcataaga tccaggccaa gtaccttgac cagatggaag atttgtacga agatttccac 901 atcgtgaagc tgccgctgct gccgcacgaa gttcgcggcg cggacaaagt taacactttc 961 tctgcgctgc tgttagaacc gtacaaaccg ccgtctgcgc agtagcacag ctgccaaccg 1021 cagccactgc cttttcatac tcacccgccg cctagcccac cgccgcatgg ccgcgtgtgt 1081 accaaatccc cgccgtaata ccgtggcagt cacctgggcc gtcgtcaagg tggtgtacac 1141 tcaccgtggt gggggtggtg gggcgcggta gtggccccgt atctgtctca cctgctgctg 1201 tttaacaaat gaagttaata ctagagatta aagaggagaa tactagatgc tgctggcggg 1261 tgctatcttc gtgctgacta tcgttctggt gatttggcag ccgaaaggtc tgggcattgg 1321 ctggtcggcg actctgggcg ctgtgctggc tctggtgacc ggcgtggtgc acccgggtga 1381 catcccagta gtttggaaca tcgtatggaa tgccacggcg gcttttattg cggttattat 1441 catctccctg ctgttggacg aaagcggttt cttcgaatgg gctgccctgc agcgttgggg 1501 taacggtcgt gggcttttca cctggattgt gctgctgggc gcggccgttg cagcgctgtt 1561 tgcgaacgat ggtgctgcac tgattctgac cccgatcgtc attgcgacgc tgctggagct 1621 gggtttctcg aaaggtacta ccctggcgtt cgtaatggct gcgggcttta tcgcggacac 1681 tgcatcttta ccgctgatcg tatctaacct ggtgagcatc gtatctgcgg acttcttcgg 1741 cctgggtttt cgcgagtacg cttctgtaat ggtcccagtg gacattgctg caatcgttgc 1801 gactctcgtg atgctgcacc tgtacttccg taaggatatc cctcagaact acgatatggc 1861 actgctgaaa tcgccggcgg aggctatcaa agatccggct acgttcaaga cgggttgggt 1921 cgtactgctg ctcctgctgg taggtctttt cgtgcttgaa ccgctgggta tccctgcaat 1981 cgcagcggta ggtgcgctga tccttttcgt tgtcgcaaaa cgcggccacg ctatcaacac 2041 tggcaaggta ctgcgtggtg ctccgtggca gattgtgatc ttctctctgg gtatgtatct 2101 tgtcgtgtac ggcctgcgta acgcgggact gactgagtac ctgtccggcg tgctgaacgt 2161 tctggccgac aacggtctgt gggctgccac tctgggcact ggtttcctga ccgcgttcct 2221 gtcgagcatt atgaacaaca tgccgacggt gctggttggt gccctgtcca ttgacggctc 2281 aactgcgtcc ggtgtgatca aagaagcgat ggtatacgca aacgtgatcg gttgcgattt 2341 gggcccgaaa atcactccaa ttggttctct ggcgaccctg ctgtggctgc acgtactttc 2401 ccagaaaaac atgaccatca gctggggtta ttactttcgt accgggatta tcatgaccct 2461 tccagtactg ttcgttactc tggccgcgct ggcgctctcc ttcactctgt aatactagag 2521 cgcaaaaaac cccgcttcgg cggggttttt tcgc //