BBa_M36221 1 arsB Ars B gene 2011-12-06T12:00:00Z 2015-05-08T01:14:03Z fd Arsenite permease, inner membrane protein responsible for forming the anion-conducting channel false false _848_ 0 11057 9 Not in stock false nbn false Lan Terry Xu, Martha Roman, Cyonna Holmes annotation2166445 1 Restriction Site BsmBI range2166445 1 268 274 annotation2166447 1 Restriction Site BsaI range2166447 1 735 741 annotation2166412 1 start codon ATG range2166412 1 1 3 BBa_B1002 1 BBa_B1002 Terminator (artificial, small, %T~=85%) 2006-08-29T11:00:00Z 2015-08-31T04:07:21Z antiquity Artifical terminator, estimated %T~=85 false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues. true Haiyao Huang annotation1898415 1 Poly A tail range1898415 1 4 9 annotation1898414 1 Poly A tail range1898414 1 25 31 annotation1898413 1 stem loop range1898413 1 10 25 annotation1898412 1 B1002 range1898412 1 1 34 BBa_M36220 1 arsA arsA gene 2011-12-06T12:00:00Z 2015-05-08T01:14:03Z Genecopoeia. http://www.genecopoeia.com/gene/hs-asna1.html 27 October 2011. This part, ArsA, comes from a homolog optimized for Homo sapien jaDHKSGdf false false _848_ 0 11057 9 Not in stock false asdgdfhfh false Designed by Terry Xu, Martha Roman, Cyonna Holmes annotation2166411 1 Start codon ATG range2166411 1 1 3 annotation2166446 1 Mutation: Restriction site BsmbI removal range2166446 1 475 480 annotation2166444 1 Mutation:Restriction site BbsI removal range2166444 1 313 318 BBa_M36222 1 BBa_M36222 ArsAction: Arsenite Removal Device (input: PoPs Signal, output: removal device) 2011-12-06T12:00:00Z 2015-05-08T01:14:04Z asfasdfasdfas The arsenic removal device is designed to prolong cell survival and decrease the rate of cell death due to arsenite toxicity. Our device does this by removing arsenite from the comprimsed cell using a two part removal method. The first coding sequence, arsA, is an ATPase that localizes to the membrane of the cell and confers energy to ArsB. ArsB uses this energy to form an ion efflux pump designed to drive arsenite out of the cell. This device is to be used as an actuator and it responds to the universal PoPs signal. The genes used are specific to the arsenic family and can only be used to create a pump that removes the ion form of arsenic (arsenite). false false _848_ 0 11057 9 Not in stock false In designing this part, we had to take into consideration the form of arsenic we wanted to remove. Removing arsenic from a cell called for the insertion of another coding sequence that coded for the gene, arsC. This addition would have significantly increased the total base pair count of our device. Instead, we decided to have our device remove arsenite. Furthermore, the ars family of genes is usually regulated by the arsR operon, but we did not including a coding sequence for arsR in our design. Because we designed our actuator to respond to the PoPS signal, we decided that inclusion of the arsR operon as a regulatory operon would be redundant. Therefore, we removed it. Also, it was necessary to codon optimize each part of our device in order to ensure successful transformation and use in the K-12 strain of E. Coli. false Designed by Cyonna Holmes, Martha Roman, Terry Xu component2166693 1 BBa_B1002 component2166684 1 BBa_B0035 component2166679 1 BBa_B0034 component2166688 1 BBa_M36221 component2166683 1 BBa_M36220 annotation2166684 1 BBa_B0035 range2166684 1 1227 1240 annotation2166688 1 BBa_M36221 range2166688 1 1247 2512 annotation2166693 1 BBa_B1002 range2166693 1 2521 2554 annotation2166683 1 BBa_M36220 range2166683 1 19 1218 annotation2166679 1 BBa_B0034 range2166679 1 1 12 BBa_B0035 1 BBa_B0035 RBS (B0030 derivative) 2004-01-27T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 Not in stock false false Jason Kelly BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B1002_sequence 1 cgcaaaaaaccccgcttcggcggggttttttcgc BBa_B0034_sequence 1 aaagaggagaaa BBa_B0035_sequence 1 attaaagaggagaa BBa_M36221_sequence 1 atgctgctggcgggtgctatcttcgtgctgactatcgttctggtgatttggcagccgaaaggtctgggcattggctggtcggcgactctgggcgctgtgctggctctggtgaccggcgtggtgcacccgggtgacatcccagtagtttggaacatcgtatggaatgccacggcggcttttattgcggttattatcatctccctgctgttggacgaaagcggtttcttcgaatgggctgccctgcagcgttggggtaacggtcgtgggcttttcacctggattgtgctgctgggcgcggccgttgcagcgctgtttgcgaacgatggtgctgcactgattctgaccccgatcgtcattgcgacgctgctggagctgggtttctcgaaaggtactaccctggcgttcgtaatggctgcgggctttatcgcggacactgcatctttaccgctgatcgtatctaacctggtgagcatcgtatctgcggacttcttcggcctgggttttcgcgagtacgcttctgtaatggtcccagtggacattgctgcaatcgttgcgactctcgtgatgctgcacctgtacttccgtaaggatatccctcagaactacgatatggcactgctgaaatcgccggcggaggctatcaaagatccggctacgttcaagacgggttgggtcgtactgctgctcctgctggtaggtcttttcgtgcttgaaccgctgggtatccctgcaatcgcagcggtaggtgcgctgatccttttcgttgtcgcaaaacgcggccacgctatcaacactggcaaggtactgcgtggtgctccgtggcagattgtgatcttctctctgggtatgtatcttgtcgtgtacggcctgcgtaacgcgggactgactgagtacctgtccggcgtgctgaacgttctggccgacaacggtctgtgggctgccactctgggcactggtttcctgaccgcgttcctgtcgagcattatgaacaacatgccgacggtgctggttggtgccctgtccattgacggctcaactgcgtccggtgtgatcaaagaagcgatggtatacgcaaacgtgatcggttgcgatttgggcccgaaaatcactccaattggttctctggcgaccctgctgtggctgcacgtactttcccagaaaaacatgaccatcagctggggttattactttcgtaccgggattatcatgacccttccagtactgttcgttactctggccgcgctggcgctctccttcactctgtaa BBa_M36220_sequence 1 atgctgctcgatgtagaaccgctggaaccgaccctctcgaacattatcgagcagcgctctcttaaatggatcttcgttggcggtaaaggtggcgtaggtaagaccacctgctcttgctctctggcagttcagctgtccaaaggtcgcgagtccgtgctcatcatctcgaccgatccggcccacaacatttcggacgcgttcgatcagaaatttagtaaagttccaaccaaggttaagggctacgataacctctttgctatggagatcgacccaagcctcggtgtcgcggatgtgccggacgaattcttcgagaacatgctgtctatgggaaaaaaaatgatgcaggaggcaatgtccgcgttcccgggtatcgatgaagccatgagctacgcggaagtcatgcgtctggttaaaggtatgaacttcagcgttgtggtattcgataccgcgccaaccggtcataccctgctgaatttcccgaccattgttgaacgtggtctgggtcgcctgatgcagatcaaaaatcagatcagtccattcatttcacagatgtgcaacatgctgggtctgggtgacatgaacgcggatcagcttgcatccaaactggaggaaacccttcctgttatccgtagcgttagcgaacaatttaaagatccggaacaaacgacttttatttgcgtttgtatcgcggaattcctgtccctgtacgaaaccgaacgtctgatccaggaactggccaaatgtaaaattgacacccataacatcatcgtaaatcagctggtgttccctgacccggaaaaaccgtgcaaaatgtgtgaagcccgtcataagatccaggccaagtaccttgaccagatggaagatttgtacgaagatttccacatcgtgaagctgccgctgctgccgcacgaagttcgcggcgcggacaaagttaacactttctctgcgctgctgttagaaccgtacaaaccgccgtctgcgcagtagcacagctgccaaccgcagccactgccttttcatactcacccgccgcctagcccaccgccgcatggccgcgtgtgtaccaaatccccgccgtaataccgtggcagtcacctgggccgtcgtcaaggtggtgtacactcaccgtggtgggggtggtggggcgcggtagtggccccgtatctgtctcacctgctgctgtttaacaaatgaagttaa BBa_M36222_sequence 1 aaagaggagaaatactagatgctgctcgatgtagaaccgctggaaccgaccctctcgaacattatcgagcagcgctctcttaaatggatcttcgttggcggtaaaggtggcgtaggtaagaccacctgctcttgctctctggcagttcagctgtccaaaggtcgcgagtccgtgctcatcatctcgaccgatccggcccacaacatttcggacgcgttcgatcagaaatttagtaaagttccaaccaaggttaagggctacgataacctctttgctatggagatcgacccaagcctcggtgtcgcggatgtgccggacgaattcttcgagaacatgctgtctatgggaaaaaaaatgatgcaggaggcaatgtccgcgttcccgggtatcgatgaagccatgagctacgcggaagtcatgcgtctggttaaaggtatgaacttcagcgttgtggtattcgataccgcgccaaccggtcataccctgctgaatttcccgaccattgttgaacgtggtctgggtcgcctgatgcagatcaaaaatcagatcagtccattcatttcacagatgtgcaacatgctgggtctgggtgacatgaacgcggatcagcttgcatccaaactggaggaaacccttcctgttatccgtagcgttagcgaacaatttaaagatccggaacaaacgacttttatttgcgtttgtatcgcggaattcctgtccctgtacgaaaccgaacgtctgatccaggaactggccaaatgtaaaattgacacccataacatcatcgtaaatcagctggtgttccctgacccggaaaaaccgtgcaaaatgtgtgaagcccgtcataagatccaggccaagtaccttgaccagatggaagatttgtacgaagatttccacatcgtgaagctgccgctgctgccgcacgaagttcgcggcgcggacaaagttaacactttctctgcgctgctgttagaaccgtacaaaccgccgtctgcgcagtagcacagctgccaaccgcagccactgccttttcatactcacccgccgcctagcccaccgccgcatggccgcgtgtgtaccaaatccccgccgtaataccgtggcagtcacctgggccgtcgtcaaggtggtgtacactcaccgtggtgggggtggtggggcgcggtagtggccccgtatctgtctcacctgctgctgtttaacaaatgaagttaatactagagattaaagaggagaatactagatgctgctggcgggtgctatcttcgtgctgactatcgttctggtgatttggcagccgaaaggtctgggcattggctggtcggcgactctgggcgctgtgctggctctggtgaccggcgtggtgcacccgggtgacatcccagtagtttggaacatcgtatggaatgccacggcggcttttattgcggttattatcatctccctgctgttggacgaaagcggtttcttcgaatgggctgccctgcagcgttggggtaacggtcgtgggcttttcacctggattgtgctgctgggcgcggccgttgcagcgctgtttgcgaacgatggtgctgcactgattctgaccccgatcgtcattgcgacgctgctggagctgggtttctcgaaaggtactaccctggcgttcgtaatggctgcgggctttatcgcggacactgcatctttaccgctgatcgtatctaacctggtgagcatcgtatctgcggacttcttcggcctgggttttcgcgagtacgcttctgtaatggtcccagtggacattgctgcaatcgttgcgactctcgtgatgctgcacctgtacttccgtaaggatatccctcagaactacgatatggcactgctgaaatcgccggcggaggctatcaaagatccggctacgttcaagacgggttgggtcgtactgctgctcctgctggtaggtcttttcgtgcttgaaccgctgggtatccctgcaatcgcagcggtaggtgcgctgatccttttcgttgtcgcaaaacgcggccacgctatcaacactggcaaggtactgcgtggtgctccgtggcagattgtgatcttctctctgggtatgtatcttgtcgtgtacggcctgcgtaacgcgggactgactgagtacctgtccggcgtgctgaacgttctggccgacaacggtctgtgggctgccactctgggcactggtttcctgaccgcgttcctgtcgagcattatgaacaacatgccgacggtgctggttggtgccctgtccattgacggctcaactgcgtccggtgtgatcaaagaagcgatggtatacgcaaacgtgatcggttgcgatttgggcccgaaaatcactccaattggttctctggcgaccctgctgtggctgcacgtactttcccagaaaaacatgaccatcagctggggttattactttcgtaccgggattatcatgacccttccagtactgttcgttactctggccgcgctggcgctctccttcactctgtaatactagagcgcaaaaaaccccgcttcggcggggttttttcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z