BBa_M36144 1 BBa_M36144 EP-B2 His-tag 2015-10-21T11:00:00Z 2015-12-03T12:46:17Z Tag Tag optimized for attachment to EP-B2. Optimal for removal during purification. false false _848_ 29086 29086 9 false Needed optimal tag for EP-B2 false Tatyanna Dadabbo & Richard Hojel & Soraya Karimi BBa_M36243 1 BBa_M36243 Pro EP-B2 YebF Secretion Device 2015-10-21T11:00:00Z 2015-12-03T12:48:09Z Composite Production of EP-B2 using both the inhibitor and EP-B2 false false _848_ 29086 29086 9 false Testing whether Pro EP-B2 is necessary to produce the EP-B2 protein. false Tatyanna Dadabbo & Richard Hojel & Soraya Karimi component2478260 1 BBa_M36120 component2478263 1 BBa_M36144 component2478264 1 BBa_M36935 component2478262 1 BBa_M36143 component2478261 1 BBa_K1510009 component2478258 1 BBa_M36136 annotation2478262 1 BBa_M36143 range2478262 1 805 1854 annotation2478258 1 BBa_M36136 range2478258 1 1 151 annotation2478264 1 BBa_M36935 range2478264 1 1928 2018 annotation2478263 1 BBa_M36144 range2478263 1 1863 1919 annotation2478261 1 BBa_K1510009 range2478261 1 256 796 annotation2478260 1 BBa_M36120 range2478260 1 160 247 BBa_M36136 1 BBa_M36136 Rhamnose-inducible promoter 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z The rhamnose-inducible promoter is described in Giacolone et al., Biotechniques 2006. We used bases 1951-2101 from BBa_K564001. This is a rhamnose-inducible promoter. We used bases 1951-2101 from BBa_K564001. It is induced by 1000 micromolar rhamnose. false false _848_ 0 13700 9 Not in stock false We used bases 1951-2101 from BBa_K564001. false Vir Choksi BBa_M36143 1 BBa_M36143 Inhibitor I29 Attached to Cysteine Proteinase EP-B2 2015-10-21T11:00:00Z 2015-12-03T12:45:40Z Barley Genome Inhibitor attached to EP-B2 to create precursor for mature EP-B2, referred to as Pro EP-B2. false false _848_ 29086 29086 9 false Testing if inhibitor is necessary for survival of cells. false Tatyanna Dadabbo & Richard Hojel & Soraya Karimi BBa_M36120 1 BBa_M36120 5' Bicistronic UTR (strong) contains ATG start Codon. 2013-10-24T11:00:00Z 2015-05-08T01:14:03Z This is based on research done in the paper "Precise and reliable gene expression via standard transcription and translation initiation elements." Used in place of an RBS. This device contains two RBS's to provide more reliability in gene selection. Place upsteam of your gene of interest in an actuator design false false _848_ 0 19058 9 Not in stock false We had to consider the strength in the design of this sequence. Because our protein ( human myoglobin) is not shown to be toxic to e. coli, we want in to be expressed in high numbers and thus we chose a strong BCD. false Alaina Shumate annotation2370704 1 misc range2370704 1 1 88 BBa_K1510009 1 BBa_K1510009 E.coli Signal sequence YebF 2014-10-05T11:00:00Z 2015-05-08T01:10:48Z The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction. Finally, the terminator B0015 is from 2014 igem distribution. This part is composed with a strong promoter, together with ribosomal binding site and signal sequence yebF, finally a double terminator. As a signal protein, YebF would be secreted outside E.coli. The role of the circuit in our project is basically a control circuit. By contrasting the result of this circuit and the other circuit, containing functional protein, we can assume if the protein works more accurately. false false _1890_ 0 21268 9 It's complicated false To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part. false Chien-Yung Huang BBa_M36935 1 BBa_M36935 Term_PhageT7 2014-10-22T11:00:00Z 2015-05-08T01:14:07Z DNA 2.0 Terminator recommended by DNA 2.0. false false _848_ 0 24151 9 Not in stock false None. false Emily Kelly BBa_M36144_sequence 1 aatagcagcagcgttgataaactggcagcagcactggaacatcatcatcatcatcat BBa_M36935_sequence 1 accgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36136_sequence 1 gggaaaaagcgggaaatgcggacgacatcacaccggcctattagtagaaactgtgaacgctatcacgttcatctttgccttgttgccagcggctcattttcctgtcagtaacgagaaggtaggtctttgagggcttttttagactgtgcgc BBa_M36143_sequence 1 gatccgtgtagcgcaattccgatggaagataaagatctggaaagtgaagaagcactgtgggatctgtatgaacgttggcagagcgcacatcgtgttcgtcgtcatcatgcagaaaaacatcgtcgctttggtacctttaaaagcaatgcacattttattcatagccataataaacgtggtgatcatccttatcgtctgcatctgaatcgttttggtgatatggatcaggcagaatttcgtgcaacctttgttggtgatctgcgtcgtgataccccgagcaaaccgccgagtgttccgggttttatgtatgcagcactgaatgttagcgatctgccgccgagcgttgattggcgtcagaaaggtgcagttaccggtgttaaagatcagggtaaatgtggtagctgttgggcatttagcaccgttgttagtgttgaaggtattaatgcaattcgtaccggtagcctggttagcctgagcgaacaggaactgattgattgtgataccgcagataatgatggttgtcagggtggtctgatggataatgcatttgaatatattaaaaataatggtggtttaattaccgaagcagcatatccgtatcgcgcagcacgtggtacctgtaatgttgcccgtgcagcacagaatagcccggttgttgttcatattgatggtcatcaggatgttccggcaaatagcgaagaagatctggcacgcgcagttgcaaatcagccggtgagcgttgcagttgaagcaagcggtaaagcatttatgttttatagcgaaggtgtttttaccggtgaatgtggtaccgaactggatcatggtgtggcagttgttggttatggtgttgcagaagatggcaaagcatattggaccgttaaaaatagctggggtccgagctggggtgaacagggttatattcgtgttgaaaaagatagcggtgcaagcggtggtctgtgtggtattgcaatggaagcaagctatccggttaaaacctatagcaaaccgaaaccgaccccgcgtcgtgcactgggtgcacgtgaaagcctg BBa_M36243_sequence 1 gggaaaaagcgggaaatgcggacgacatcacaccggcctattagtagaaactgtgaacgctatcacgttcatctttgccttgttgccagcggctcattttcctgtcagtaacgagaaggtaggtctttgagggcttttttagactgtgcgctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggatccgtgtagcgcaattccgatggaagataaagatctggaaagtgaagaagcactgtgggatctgtatgaacgttggcagagcgcacatcgtgttcgtcgtcatcatgcagaaaaacatcgtcgctttggtacctttaaaagcaatgcacattttattcatagccataataaacgtggtgatcatccttatcgtctgcatctgaatcgttttggtgatatggatcaggcagaatttcgtgcaacctttgttggtgatctgcgtcgtgataccccgagcaaaccgccgagtgttccgggttttatgtatgcagcactgaatgttagcgatctgccgccgagcgttgattggcgtcagaaaggtgcagttaccggtgttaaagatcagggtaaatgtggtagctgttgggcatttagcaccgttgttagtgttgaaggtattaatgcaattcgtaccggtagcctggttagcctgagcgaacaggaactgattgattgtgataccgcagataatgatggttgtcagggtggtctgatggataatgcatttgaatatattaaaaataatggtggtttaattaccgaagcagcatatccgtatcgcgcagcacgtggtacctgtaatgttgcccgtgcagcacagaatagcccggttgttgttcatattgatggtcatcaggatgttccggcaaatagcgaagaagatctggcacgcgcagttgcaaatcagccggtgagcgttgcagttgaagcaagcggtaaagcatttatgttttatagcgaaggtgtttttaccggtgaatgtggtaccgaactggatcatggtgtggcagttgttggttatggtgttgcagaagatggcaaagcatattggaccgttaaaaatagctggggtccgagctggggtgaacagggttatattcgtgttgaaaaagatagcggtgcaagcggtggtctgtgtggtattgcaatggaagcaagctatccggttaaaacctatagcaaaccgaaaccgaccccgcgtcgtgcactgggtgcacgtgaaagcctgtactagagaatagcagcagcgttgataaactggcagcagcactggaacatcatcatcatcatcattactagagaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggaggaactatatccgggtaac BBa_M36120_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg BBa_K1510009_sequence 1 ttgacggctagctcagtcctaggtacagtgctagctactagggagaaaaacatgaaaaaaagaggggcgtttttagggctgttgttggtttctgcctgcgcatcagttttcgctgccaataatgaaaccagcaagtcggtcactttcccaaagtgtgaagatctggatgctgccggaattgccgcgagcgtaaaacgtgattatcaacaaaatcgcgtggcgcgttgggcagatgatcaaaaaattgtcggtcaggccgatcccgtggcttgggtcagtttgcaggacattcagggtaaagatgataaatggtcagtaccgctaaccgtgcgtggtaaaagtgccgatattcattaccaggtcagcgtggactgcaaagcgggaatggcggaatatcagcggcgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z