BBa_M36246 1 BBa_M36246 Cadmium Sensor 2011-12-10T12:00:00Z 2015-05-08T01:14:04Z Watson N., et al, (1992) Identification of Elements Involve in Transcriptional Regulation of the Escherichia coli cad Operon by External pH, Journal of Bacteriology, Vol 174, p. 530-540. biofab.org Keselr, I., Collado-Vides, J., Santos-Zavaleta, A., Peralta-Gil, M., Gama-Castro, S., Muniz-Rascado, L., Bonavides-Martinez, C., Paley, S., Krummenacker, M., Altman, T., Kaipa, P., Spaulding, A., Pacheco, J., Latendresse, M., Fulcher, C., Sarker, M., Shearer, A., Mackie A., Paulsen, I., Gunsalus., and Karp, P., (2010), EcoCyc: a comprehensive database of Escherichia coli biology. Escherichia coli str. K-12 substr. MG1655 chromosome, complete genome. NCBI Reference Sequence: NC_000913.2. This part was designed to promote the CadC gene, taken from E.Coli, constitutively with a bicistronic ribosome binding site. CadC serves as a transcription factor for the PCad promoter sequence. PCad serves to produce a PoPs signal that can then be used as an input for a sensor device. In order to detect Cadmium, we used a cadmium sensor from E.Coli. The genome of E.Coli codes for three genes, CadC, CadA, and CadB which allow for some resistance to cadmium. CadC, BBa_M36244, is a transcription factor that along with cadmium binds to a promoter, called Pcad, BBa_M36245, that promotes CadA and CadB. By using Pcad as a promoter, we can control the expression of any gene that follows it, using cadmium. To express Pcad, we also need CadC. We constitutively expressed CadC with a medium strength promoter from biofab.org, apFAB64. Following the promoter is a bicistronic ribosome binding site, BD2, BBa_M36282. CadC is already produced in E.Coli in small amounts, and more so with cadmium present. Our plasmid???s Pcad promoter may use either the CadC expressed from our plasmid or expressed naturally in E.Coli. Either way, Pcad will sense the presence of cadmium. After CadC, we placed a strong terminator, apFAB381. false false _848_ 0 11062 9 Not in stock false During design, we used a medium strength promoter to ensure the promoter did not over work the cell. false Bianca Kapoor, Sally Winkler component2166803 1 BBa_M36282 component2166805 1 BBa_M36248 component2166804 1 BBa_M36244 component2166801 1 BBa_M36247 component2166806 1 BBa_M36245 annotation2166805 1 BBa_M36248 range2166805 1 1687 1768 annotation2166804 1 BBa_M36244 range2166804 1 140 1678 annotation2166801 1 BBa_M36247 range2166801 1 1 37 annotation2166803 1 BBa_M36282 range2166803 1 46 133 annotation2166806 1 BBa_M36245 range2166806 1 1777 1911 BBa_M36245 1 BBa_M36245 PCad Promoter 2011-12-09T12:00:00Z 2015-05-08T01:14:04Z Comes from E.Coli genome. This promoter, taken from E.Coli is designed with the CadC protein as a transcription factor. In nature, it lies upstream of the CadA and CadB genes which code for cadmium resistance. false false _848_ 0 11062 9 Not in stock false Ensuring to encode entire CadC binding site through +1 site for transcription. false Bianca Kapoor, Sally Winkler BBa_M36248 1 BBa_M36248 Strong Terminator 2011-12-10T12:00:00Z 2015-05-08T01:14:04Z biofab.org part number: apFAB381 This codes for a strong terminator. false false _848_ 0 11062 9 Not in stock false used in E.Coli. false Bianca Kapoor BBa_M36282 1 BBa_M36282 5' Bicistronic UTR, includes ATG start codon 2011-11-29T12:00:00Z 2015-05-08T01:14:04Z BioFab: apFAB563 This sequence contains an RBS followed by the sequence TAATG. The parts number is BBa_M36000 (PartsRegistry). The first RBS sequence is strong, while the second one is weak. This stops the first polymerase with the stop codon TAA, but allows for a second polymerase to bind to the start codon ATG(BIOFAB). This sequence prevents a hairpin loop from being formed with another part of the sequence, which would decrease the amount of translation. The bicistronic sequence ends with a start codon ATG. false true _848_ 0 11068 9 Not in stock false no false Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso annotation2166677 1 Start Codon range2166677 1 86 88 BBa_M36244 1 BBa_M36244 CadC Transcription Factor 2011-12-09T12:00:00Z 2015-05-08T01:14:04Z Comes from E.Coli genome normally encoding for cadmium resistance This part produces the CadC gene which can bind to the PCad promoter and act as inducer false false _848_ 0 11062 9 Not in stock false Ensuring we included all of CadC, but not CadA or CadB false Bianca Kapoor, Sally Winkler BBa_M36247 1 BBa_M36247 Constitutive Promoter 2011-12-10T12:00:00Z 2015-09-08T05:50:45Z biofab.org part number: apFAB64 This part promotes constitutively at medium strength. false false _848_ 21980 11062 9 Not in stock false none false Bianca Kapoor BBa_M36246_sequence 1 ttgacatcaggaaaatttttctgatacttacagccattactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagatgcaacaacctgtagttcgcgttggcgaatggcttgttactccgtccataaaccaaattagccgcaatgggcgtcaacttacccttgagccgagattaatcgatcttctggttttctttgctcaacacagtggcgaagtacttagcagggatgaacttatcgataatgtctggaagagaagtattgtcaccaatcacgttgtgacgcagagtatctcagaactacgtaagtcattaaaagataatgatgaagatagtcctgtctatatcgctactgtaccaaagcgcggctataaattaatggtgccggttatctggtacagcgaagaagagggagaggaaataatgctatcttcgcctccccctataccagaggcggttcctgccacagattctccctcccacagtcttaacattcaaaacaccgcaacgccacctgaacaatccccagttaaaagcaaacgattcactaccttttgggtatggttttttttcctgttgtcgttaggtatctgtgtagcactggtagcgttttcaagtcttgatacacgtcatcctatgagcaaatcgcgtattttgctcaatccacgcgatattgacattaatatggtaaataaaagttgtaacagctggagttccccgtatcagctctcttacgcgataggcgtgggtgatttggtggcgacatcacttaacaccttctccacctttatggtgcatgacaaaatcaactacaacattgatgaaccgagcagttccggtaaaacattatctattgcgtttgttaatcagcgccaataccgtgctcaacaatgctttatgtcgataaaattggtagacaatgcagatggttcaaccatgctggataaacgttatgtcatcactaacggtaatcagctggcgattcaaaatgatttactggagagtttatcaaaagcgttaaaccaaccgtggccacaacgaatgcaggagatgctccagaaaattttgccgcatcgtggtgcgttattaactaatttttatcaggcacatgattatttactgcatggcgatgataaatcattgaaccgtgccagtgaattattaggtgagattgttcaatcatccccagaatttacctacgcgagagcagaaaaagcattagttgatatcgtgcgccattctcaacatcctttagatgaaaaacaattagcagcactgaacacagaaatagataacattgttacactgccggaattgaacaacctgtccattatatatcaaataaaagcggtcagtgctctggtaaaaggtaaaacagatgagtcttaccaggcgataaatactggcattgatcttgaaatgtcctggctaaattatgtgttgcttggcaaggtttatgaaatgaaggggatgaaccgggaagcagctgatgcatatctcaccgcctttaatttacgcccaggggcaaacaccctttactggattgaaaatggtatattccagacttctgttccttatgttgtaccttatctcgacaaatttcttgcttcagaataatactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctactagagtttgtgttatttcacctaatctttaggattaatccttttttcgtgagtaatcttatcgccagtttggtctggtcaggaaatagttatacatcatgacccggactccaaattcaaaaatgaaattaggagaagagc BBa_M36244_sequence 1 atgcaacaacctgtagttcgcgttggcgaatggcttgttactccgtccataaaccaaattagccgcaatgggcgtcaacttacccttgagccgagattaatcgatcttctggttttctttgctcaacacagtggcgaagtacttagcagggatgaacttatcgataatgtctggaagagaagtattgtcaccaatcacgttgtgacgcagagtatctcagaactacgtaagtcattaaaagataatgatgaagatagtcctgtctatatcgctactgtaccaaagcgcggctataaattaatggtgccggttatctggtacagcgaagaagagggagaggaaataatgctatcttcgcctccccctataccagaggcggttcctgccacagattctccctcccacagtcttaacattcaaaacaccgcaacgccacctgaacaatccccagttaaaagcaaacgattcactaccttttgggtatggttttttttcctgttgtcgttaggtatctgtgtagcactggtagcgttttcaagtcttgatacacgtcatcctatgagcaaatcgcgtattttgctcaatccacgcgatattgacattaatatggtaaataaaagttgtaacagctggagttccccgtatcagctctcttacgcgataggcgtgggtgatttggtggcgacatcacttaacaccttctccacctttatggtgcatgacaaaatcaactacaacattgatgaaccgagcagttccggtaaaacattatctattgcgtttgttaatcagcgccaataccgtgctcaacaatgctttatgtcgataaaattggtagacaatgcagatggttcaaccatgctggataaacgttatgtcatcactaacggtaatcagctggcgattcaaaatgatttactggagagtttatcaaaagcgttaaaccaaccgtggccacaacgaatgcaggagatgctccagaaaattttgccgcatcgtggtgcgttattaactaatttttatcaggcacatgattatttactgcatggcgatgataaatcattgaaccgtgccagtgaattattaggtgagattgttcaatcatccccagaatttacctacgcgagagcagaaaaagcattagttgatatcgtgcgccattctcaacatcctttagatgaaaaacaattagcagcactgaacacagaaatagataacattgttacactgccggaattgaacaacctgtccattatatatcaaataaaagcggtcagtgctctggtaaaaggtaaaacagatgagtcttaccaggcgataaatactggcattgatcttgaaatgtcctggctaaattatgtgttgcttggcaaggtttatgaaatgaaggggatgaaccgggaagcagctgatgcatatctcaccgcctttaatttacgcccaggggcaaacaccctttactggattgaaaatggtatattccagacttctgttccttatgttgtaccttatctcgacaaatttcttgcttcagaataa BBa_M36247_sequence 1 ttgacatcaggaaaatttttctgatacttacagccat BBa_M36282_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg BBa_M36248_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36245_sequence 1 tttgtgttatttcacctaatctttaggattaatccttttttcgtgagtaatcttatcgccagtttggtctggtcaggaaatagttatacatcatgacccggactccaaattcaaaaatgaaattaggagaagagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z