BBa_M36557
1
BBa_M36557
VItamin D2 Actuator
2012-12-04T12:00:00Z
2015-05-08T01:14:05Z
This part was inspired from the following paper ( Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.???Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe???.7 December 2007) a direct link is available below http://mic.sgmjournals.org/content/154/3/830.full
This device converts ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. In the presence of UV light ergosterol is converted to Vitamin D2. The coding region[BBa_M36655] produces C-24(28) sterol reductase an enzyme that catalyzes the final step in Vitamin D2 synthesis pathway by converting ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol.
false
true
_848_
0
15023
9
Not in stock
false
This construct is uses a strong RBS and 5'UTR so that considerable amounts of C-24(28) sterol reductase to convert the precursor to ergosterol.
false
Elvis Ikwa,Alexis Garduno, Johnathan Bowes
component2213635
1
BBa_B0015
component2213624
1
BBa_M36724
component2213628
1
BBa_M36655
annotation2213624
1
BBa_M36724
range2213624
1
1
85
annotation2213635
1
BBa_B0015
range2213635
1
1462
1590
annotation2213628
1
BBa_M36655
range2213628
1
92
1453
BBa_M36724
1
BBa_M36724
5' Bicistronic UTR (strong), does not include ATG start
2011-04-27T11:00:00Z
2015-05-08T01:14:06Z
Vivek Mutalik et al. c/o BIOFAB Emeryville.
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
false
false
_848_
0
52
432
Not in stock
false
Vivek says, "This is (the) Makoff design, it gives good expression and is seq independent of the Schoner design."
false
Drew Endy
BBa_M36655
1
BBa_M36655
C-24(28) sterol reductase
2012-12-04T12:00:00Z
2015-05-08T01:14:05Z
This sequence is derived from Schizosaccharomyces pombe 972h- (strain: 972h-). Gene ID :2541740 in the NCBI database.
Enzyme coding sequence for C-24(28) sterol reductase, an enzyme which acts in the last step of Vitamin D2 synthesis by reducing ergosta-5,7,22,24(28)-tetraenol to ergosterol. No mutations were made to the enzymes genetic sequence. The genetic sequence is optimized for use in Synechococcus elongatus (PCC 7942).
false
false
_848_
0
15023
9
Not in stock
false
The coding sequence denoted by Gene ID :2541740 has to be codon optimized for Synechococcus elongatus.
false
Elvis Ikwa, Alexis Garduno, Johnathan Bowes
annotation2213462
1
C-24(28) sterol reductase coding region
range2213462
1
1
1362
annotation2213460
1
start
range2213460
1
1
3
annotation2213461
1
Stop
range2213461
1
1360
1362
BBa_M36257
1
BBa_M36257
Vitamin D2 Actuator
2012-12-04T12:00:00Z
2015-05-08T01:14:04Z
This part was designed due to inspiration from [Tomoko Ikwaki,Haruyuki lefuji,Yoshikazu Hiraga,Akira Hosomi, Tomotake Morita, Yuko GigaHamam Kaoru Takegawa.???Multiple functions of ergosterol in the fission yeast Schizosaccharomyces pombe???.7 December 2007 ] a direct link is available below http://mic.sgmjournals.org/content/154/3/830.full
This part converts ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol. In the presence of UV light ergosterol is converted to Vitamin D2. The coding region[BBa_M36655] produces C-24(28) sterol reductase an enzyme that catalyzes the final step in Vitamin D2 synthesis pathway by converting ergosta-5,7,22,24(28)-tetraen-3β-ol to ergosterol.
true
false
_848_
0
15023
9
Discontinued
false
This construct uses a strong promoter and 5'UTR so that the amount of C-24(28) sterol reductase is substancial to efficiently convert the precursor to Vitamin D2.
false
Elvis Ikwa, Alexis Garduno, Johnathan Bowes
component2213591
1
BBa_M36724
component2213602
1
BBa_B0015
component2213595
1
BBa_M36655
annotation2213595
1
BBa_M36655
range2213595
1
92
1453
annotation2213591
1
BBa_M36724
range2213591
1
1
85
annotation2213602
1
BBa_B0015
range2213602
1
1462
1590
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M36557_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctgtactagatgaaatctacagtaaagaaatcagctcctcgtgagtttggcggtgccaaaggtgcattggcaattatgacaggattcccttgtctaatgtattacttatgggcatgctccaaatttaatgattcgcagtttattaaaccagaatcttttacgatagcaggatttcaaaatttttttagaacgttaggccattatatctacgtcggtgcctatccaacacggtatgcattcttggttttttggtcgttttgtatagttcaggctgtcatgtacttaactcttcctggtgttcggacgcaaggtttgcctttaaagcatcgcaataacgaacgtcttccttacttatgcaatgctatctggtctttttacacgacaattgttattcttgccgttcttcacgtgacccacgtatttccaattactacatttatcgacatgttcggtcctttgatgagcgttgcaataataaccgcttttgtctgtacctttgttttgtacactggaactttgctttttggtgatcgactttttgataaacctcatcggttatctggaaatcccatctacgatgcctttatgggagcttgcttgaatcctcgccttggcaaattgcttgattttaaaatgttctttgaagttcgtatcccttggtttatcctattctttatttccgttggtgctgctgttcgtcaatatgaaacttatggcactgtaagtcctcaggtacttttcgtttgtcttggccattatttgtatgcaaacgcgtgctcgaagggagagcagttgattgttccaacttgggacatggcttatgaaaagtttggttttatgcttatcttttggaacatggctggggtcccatttacttattcacactgcaccctttaccttttttcgcatgatccatctgtttacaactggtcaactcaatatacaaccggtatttatgttttgcttctttgctgttattatatatttgatacttgcaatggtcaaaaaaatcatttccgtaatcaaatttatggtactgaggttcatcgcaaaacatttccccaacttccctggcttatcatcaagaaccctacctttattcgttgtgcaaatggcggtactttgttgacttcaggttggtataggtacgctcgtaaaattcattatactgctgatttctttcaatcattgtcatgggcattgatcaccggttttcagtcccctctaccttacttttacccatgctttttctttgtagtccttgtgcatcgtgtttcacgtgatattaaaaagtgcaaagctaagtatggggctgatttcgatgagtattgccgtatatgcccttacttatttattccctatatattttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M36655_sequence
1
atgaaatctacagtaaagaaatcagctcctcgtgagtttggcggtgccaaaggtgcattggcaattatgacaggattcccttgtctaatgtattacttatgggcatgctccaaatttaatgattcgcagtttattaaaccagaatcttttacgatagcaggatttcaaaatttttttagaacgttaggccattatatctacgtcggtgcctatccaacacggtatgcattcttggttttttggtcgttttgtatagttcaggctgtcatgtacttaactcttcctggtgttcggacgcaaggtttgcctttaaagcatcgcaataacgaacgtcttccttacttatgcaatgctatctggtctttttacacgacaattgttattcttgccgttcttcacgtgacccacgtatttccaattactacatttatcgacatgttcggtcctttgatgagcgttgcaataataaccgcttttgtctgtacctttgttttgtacactggaactttgctttttggtgatcgactttttgataaacctcatcggttatctggaaatcccatctacgatgcctttatgggagcttgcttgaatcctcgccttggcaaattgcttgattttaaaatgttctttgaagttcgtatcccttggtttatcctattctttatttccgttggtgctgctgttcgtcaatatgaaacttatggcactgtaagtcctcaggtacttttcgtttgtcttggccattatttgtatgcaaacgcgtgctcgaagggagagcagttgattgttccaacttgggacatggcttatgaaaagtttggttttatgcttatcttttggaacatggctggggtcccatttacttattcacactgcaccctttaccttttttcgcatgatccatctgtttacaactggtcaactcaatatacaaccggtatttatgttttgcttctttgctgttattatatatttgatacttgcaatggtcaaaaaaatcatttccgtaatcaaatttatggtactgaggttcatcgcaaaacatttccccaacttccctggcttatcatcaagaaccctacctttattcgttgtgcaaatggcggtactttgttgacttcaggttggtataggtacgctcgtaaaattcattatactgctgatttctttcaatcattgtcatgggcattgatcaccggttttcagtcccctctaccttacttttacccatgctttttctttgtagtccttgtgcatcgtgtttcacgtgatattaaaaagtgcaaagctaagtatggggctgatttcgatgagtattgccgtatatgcccttacttatttattccctatatattttag
BBa_M36724_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M36257_sequence
1
gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcacaggagactttctgtactagatgaaatctacagtaaagaaatcagctcctcgtgagtttggcggtgccaaaggtgcattggcaattatgacaggattcccttgtctaatgtattacttatgggcatgctccaaatttaatgattcgcagtttattaaaccagaatcttttacgatagcaggatttcaaaatttttttagaacgttaggccattatatctacgtcggtgcctatccaacacggtatgcattcttggttttttggtcgttttgtatagttcaggctgtcatgtacttaactcttcctggtgttcggacgcaaggtttgcctttaaagcatcgcaataacgaacgtcttccttacttatgcaatgctatctggtctttttacacgacaattgttattcttgccgttcttcacgtgacccacgtatttccaattactacatttatcgacatgttcggtcctttgatgagcgttgcaataataaccgcttttgtctgtacctttgttttgtacactggaactttgctttttggtgatcgactttttgataaacctcatcggttatctggaaatcccatctacgatgcctttatgggagcttgcttgaatcctcgccttggcaaattgcttgattttaaaatgttctttgaagttcgtatcccttggtttatcctattctttatttccgttggtgctgctgttcgtcaatatgaaacttatggcactgtaagtcctcaggtacttttcgtttgtcttggccattatttgtatgcaaacgcgtgctcgaagggagagcagttgattgttccaacttgggacatggcttatgaaaagtttggttttatgcttatcttttggaacatggctggggtcccatttacttattcacactgcaccctttaccttttttcgcatgatccatctgtttacaactggtcaactcaatatacaaccggtatttatgttttgcttctttgctgttattatatatttgatacttgcaatggtcaaaaaaatcatttccgtaatcaaatttatggtactgaggttcatcgcaaaacatttccccaacttccctggcttatcatcaagaaccctacctttattcgttgtgcaaatggcggtactttgttgacttcaggttggtataggtacgctcgtaaaattcattatactgctgatttctttcaatcattgtcatgggcattgatcaccggttttcagtcccctctaccttacttttacccatgctttttctttgtagtccttgtgcatcgtgtttcacgtgatattaaaaagtgcaaagctaagtatggggctgatttcgatgagtattgccgtatatgcccttacttatttattccctatatattttagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z