BBa_M36268 1 BBa_M36268 LasR-N-(3-oxododecanoyl)-homoserine lactone qsc102 sensor 2014-10-23T11:00:00Z 2015-05-08T01:14:04Z See the sources documented in the individual parts of this device. Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasI produces N-(3-oxododecanoyl)-homoserine lactone, and LasR interacts with this molecule to transcriptionally regulate a variety of quorum sensing genes. One of these genes is qsc102. This composite part consists of a constitutive promoter, a RBS, the LasR gene, a terminator, and the qsc102 promoter. This entire construct is optimized for a plasmid that will be transformed into E. coli. This construct will be coupled to a standard fluorescence actuator so that in the presence of N-(3-oxododecanoyl)-homoserine lactone, the part will induce transcription of the fluorescence actuator. false false _848_ 0 24142 9 Not in stock false The device is optimized for E. coli, and a small sequence of "TAT" was entered between the RBS and the LasR gene for ideal transcription conditions false Diana Gong, Michaela Hinks component2429211 1 BBa_M36135 component2429213 1 BBa_M36265 component2429206 1 BBa_M36269 component2429203 1 BBa_M36266 component2429210 1 BBa_M36264 annotation2429211 1 BBa_M36135 range2429211 1 783 821 annotation2429210 1 BBa_M36264 range2429210 1 63 782 annotation2429203 1 BBa_M36266 range2429203 1 1 47 annotation2429213 1 BBa_M36265 range2429213 1 822 888 annotation2429206 1 BBa_M36269 range2429206 1 48 62 BBa_M36135 1 BBa_M36135 Transcription terminator (apFAB388) 2012-05-31T11:00:00Z 2015-05-08T01:14:03Z BIOFAB: part apFAB388, construct pFAB822 Released HQ 2013 Strong terminator from BIOFAB's terminator project false false _848_ 0 13700 9 In stock false Length similar to terminators used in Delebecque et al., Science 2011 false Vir Choksi BBa_M36265 1 BBa_M36265 qsc102 promoter 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z The promoter sequence was obtained from Figure 3 of this paper: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC95443/pdf/jb005529.pdf Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. When LasR interacts with N-(3-oxododecanoyl)-homoserine lactone which is produced by LasI, the complex binds to the promoter of the qsc102 gene and increases expression. false false _848_ 0 24142 9 Not in stock false N/A false Diana Gong annotation2429161 1 qsc102 promoter range2429161 1 1 67 BBa_M36264 1 BBa_M36264 LasR optimized for E. coli 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z The original LasR sequence was obtained from: http://www.uniprot.org/uniprot/P25084 This sequence was optimized for E. coli and our composite part by DNA 2.0. Quorum sensing is a system bacteria use to coordinate behaviors that correlate to cell density. Each species of bacteria produces and senses a unique, diffusable small molecule. This allows bacteria to "count" how many cells of their species are in the vicinity. Pseudomonas Aeruginosa, an opportunistic human pathogen associated with cystic fibrosis, uses two quorum sensing systems, with one of them being LasI/LasR. LasR binds to N-(3-oxododecanoyl)-homoserine lactone, which is produced by LasI, to form a complex that regulates quorum sensing-related genes. This part begins with a start codon and ends with the stop codon for the LasR gene. false false _848_ 0 24142 9 Not in stock false N/A false Diana Gong annotation2429158 1 Stop Codon range2429158 1 718 720 annotation2429159 1 LasR range2429159 1 1 720 annotation2429157 1 Start Codon range2429157 1 1 3 BBa_M36266 1 BBa_M36266 biofab promoter apFAB39 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z The promoter comes from the Modular Promoter Library from the paper: "Precise and Reliable Gene Expression via Standard Transcription and Translation Initiation" by Vivek Mutalik et al. The name of the promoter in the paper is apFAB39. This is a medium strength promoter for E. coli. In the modular promoter library from which we obtained this promoter, the fluorescent actuator response in arbitrary units ranged from 0.378777 to 897.636, and promoter apFAB39 was 594.378. false false _848_ 0 24142 9 Not in stock false N/A false Diana Gong annotation2429162 1 promoter apFAB39 range2429162 1 1 47 BBa_M36269 1 BBa_M36269 RBS: modified BBa_B0034 2014-10-23T11:00:00Z 2015-05-08T01:14:04Z This is the BBa_B0034 RBS with three extra bases added onto the end. This is a slight modification of the part BBa_B0034, a RBS that currently exists in the registry and has been frequently used. For the purposes of our device, we are annexing the sequence "TAT" onto the end of this RBS to serve as a filler between the RBS and a gene we wish to transcribe. We believe having these few extra bases between the Shine-Dalgarno sequence and the start codon of our gene of interest will optimize transcription of our gene. false false _848_ 0 24142 9 Not in stock false N/A false Diana Gong annotation2429201 1 optimization filler range2429201 1 13 15 annotation2429200 1 BBa_B0034 range2429200 1 1 12 BBa_M36265_sequence 1 acctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattg BBa_M36264_sequence 1 atggccctggttgacggctttttggaattggaacgtagctctggtaaactggagtggtcagcaatcctgcagaaaatggctagcgacctgggcttcagcaagattctgtttggtctgttgccgaaggattcccaggactacgagaacgcctttatcgtcggtaattaccctgctgcgtggcgcgagcactacgatcgtgcgggctatgcgcgtgttgacccgaccgtcagccactgcactcaaagcgttctgccgattttctgggagccgagcatctatcagacgcgcaagcaacatgaattcttcgaagaggcaagcgccgcgggcctggtgtatggtctgacgatgccgctgcatggcgcacgcggcgagctgggtgcgctgagcctgagcgtcgaagcggaaaatcgtgcagaggcgaaccgctttatcgagagcgtactgccaaccctctggatgctgaaagattacgcgctgcagtcgggtgccggtctggctttcgagcacccggtgagcaaaccggttgttttgacgtcttgggagaaagaagtcctgcaatggtgtgcgatcggtaaaaccagctgggagatcagcgtgatttgtaactgctccgaagcgaacgtgaatttccacatgggtaacattcgtcgtaagtttggcgtgaccagccgtcgtgttgccgcaattatggcagtgaatctgggtctgattaccctgtaa BBa_M36266_sequence 1 aaaaagagtattgacttcaggaaaatttttctgatacttacagccat BBa_M36135_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_M36268_sequence 1 aaaaagagtattgacttcaggaaaatttttctgatacttacagccataaagaggagaaatatatggccctggttgacggctttttggaattggaacgtagctctggtaaactggagtggtcagcaatcctgcagaaaatggctagcgacctgggcttcagcaagattctgtttggtctgttgccgaaggattcccaggactacgagaacgcctttatcgtcggtaattaccctgctgcgtggcgcgagcactacgatcgtgcgggctatgcgcgtgttgacccgaccgtcagccactgcactcaaagcgttctgccgattttctgggagccgagcatctatcagacgcgcaagcaacatgaattcttcgaagaggcaagcgccgcgggcctggtgtatggtctgacgatgccgctgcatggcgcacgcggcgagctgggtgcgctgagcctgagcgtcgaagcggaaaatcgtgcagaggcgaaccgctttatcgagagcgtactgccaaccctctggatgctgaaagattacgcgctgcagtcgggtgccggtctggctttcgagcacccggtgagcaaaccggttgttttgacgtcttgggagaaagaagtcctgcaatggtgtgcgatcggtaaaaccagctgggagatcagcgtgatttgtaactgctccgaagcgaacgtgaatttccacatgggtaacattcgtcgtaagtttggcgtgaccagccgtcgtgttgccgcaattatggcagtgaatctgggtctgattaccctgtaaaaaaaaaaaccccgcccctgacagggcggggttttttttacctgcccggaagggcaggttgtccctgccgggctgtgacaatttaattcgaccaggcatttcattg BBa_M36269_sequence 1 aaagaggagaaatat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z