BBa_M36294
type: ComponentDefinition
persistent identity: BBa M36294
display id: BBa_M36294
version: 1
was generated by: iGEM to SBOL conversion
description: Spider Silk Actuator
created: 2011-12-06T12:00:00Z
modified: 2015-05-08T01:14:04Z
mutable provenance: This sequence was used with E. Coli cells.
owned by: james
owned by: myers
mutable description: This is the full sequence for the spider silk actuator. It includes the bicistonic sequence, FOS, a linker, the silk hexamer, another linker, JUN, a stop codon, and a transcription terminator. It is triggered by PoPS.
discontinued: true
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11078
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: There were no complications when creating the full sequence. However, some parts, such as the hexamer, took special considerations which are detailed on their basic part pages.
star: false
creator: Dominique Dabija
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_M36501
component: BBa_M36500
component: BBa_M36502
component: BBa_M36293
component: BBa_M36292
component: BBa_M36394
component: BBa_M36296
sequence annotation: BBa_M36292
sequence annotation: BBa_M36293
sequence annotation: BBa_M36296
sequence annotation: BBa_M36394
sequence annotation: BBa_M36500
sequence annotation: BBa_M36501
sequence annotation: BBa_M36502
sequence: https://synbiohub.org/public/igem/BBa_M36294_sequence/1

BBa_M36501
type: Component
persistent identity: component2166486
display id: component2166486
version: 1
top level: BBa_M36294
definition: Fos
access: public

BBa_M36500
type: Component
persistent identity: component2166485
display id: component2166485
version: 1
top level: BBa_M36294
definition: spilk 7.0.
access: public

BBa_M36502
type: Component
persistent identity: component2166487
display id: component2166487
version: 1
top level: BBa_M36294
definition: Jun
access: public

BBa_M36293
type: Component
persistent identity: component2166475
display id: component2166475
version: 1
top level: BBa_M36294
definition: BCRBS
access: public

BBa_M36292
type: Component
persistent identity: component2166474
display id: component2166474
version: 1
top level: BBa_M36294
definition: BBa_M36292
access: public

BBa_M36394
type: Component
persistent identity: component2166484
display id: component2166484
version: 1
top level: BBa_M36294
definition: BBa_M36394
access: public

BBa_M36296
type: Component
persistent identity: component2166476
display id: component2166476
version: 1
top level: BBa_M36294
definition: halt
access: public

BBa_M36292
type: SequenceAnnotation
persistent identity: annotation2166474
display id: annotation2166474
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166474/range2166474/1
component: BBa_M36292

https://synbiohub.org/public/igem/BBa_M36294/annotation2166474/range2166474/1
type: Range
persistent identity: range2166474
display id: range2166474
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 1
end: 82
orientation: inline

BBa_M36293
type: SequenceAnnotation
persistent identity: annotation2166475
display id: annotation2166475
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166475/range2166475/1
component: BBa_M36293

https://synbiohub.org/public/igem/BBa_M36294/annotation2166475/range2166475/1
type: Range
persistent identity: range2166475
display id: range2166475
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 91
end: 178
orientation: inline

BBa_M36296
type: SequenceAnnotation
persistent identity: annotation2166476
display id: annotation2166476
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166476/range2166476/1
component: BBa_M36296

https://synbiohub.org/public/igem/BBa_M36294/annotation2166476/range2166476/1
type: Range
persistent identity: range2166476
display id: range2166476
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 187
end: 189
orientation: inline

BBa_M36394
type: SequenceAnnotation
persistent identity: annotation2166484
display id: annotation2166484
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166484/range2166484/1
component: BBa_M36394

https://synbiohub.org/public/igem/BBa_M36294/annotation2166484/range2166484/1
type: Range
persistent identity: range2166484
display id: range2166484
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 198
end: 1333
orientation: inline

BBa_M36500
type: SequenceAnnotation
persistent identity: annotation2166485
display id: annotation2166485
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166485/range2166485/1
component: BBa_M36500

https://synbiohub.org/public/igem/BBa_M36294/annotation2166485/range2166485/1
type: Range
persistent identity: range2166485
display id: range2166485
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 1342
end: 1971
orientation: inline

BBa_M36501
type: SequenceAnnotation
persistent identity: annotation2166486
display id: annotation2166486
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166486/range2166486/1
component: BBa_M36501

https://synbiohub.org/public/igem/BBa_M36294/annotation2166486/range2166486/1
type: Range
persistent identity: range2166486
display id: range2166486
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 1980
end: 2099
orientation: inline

BBa_M36502
type: SequenceAnnotation
persistent identity: annotation2166487
display id: annotation2166487
version: 1
top level: BBa_M36294
location: https://synbiohub.org/public/igem/BBa_M36294/annotation2166487/range2166487/1
component: BBa_M36502

https://synbiohub.org/public/igem/BBa_M36294/annotation2166487/range2166487/1
type: Range
persistent identity: range2166487
display id: range2166487
version: 1
top level: BBa_M36294
direction: direction/reverse
start: 2108
end: 2227
orientation: inline

spilk 7.0.
type: ComponentDefinition
persistent identity: BBa M36500
display id: BBa_M36500
version: 1
was generated by: iGEM to SBOL conversion
description: Spider Silk Hexamer
created: 2011-12-03T12:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: The monomer amino acid sequence was taken from Xia, X.-X., Z.-G. Qian, C. S. Ki, Y. H. Park, D. L. Kaplan, and S. Y. Lee. "Native-sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber." Proceedings of the National Academy of Sciences 107.32 (2010): 14059-4063. Print.
owned by: james
owned by: myers
mutable description: This is a spider silk hexamer protein. The monomer amino acid sequence was taken from Xia et al. and codon optimized for expression in E. coli. Repeats above 10 base pairs were also minimized to allow for ease of synthesis.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11065
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Needed to minimize repeats and allow for ideal mRNA forming without too many loops while still maintaining codon optimization for E. coli.
star: false
creator: Dominique Dabija, Christopher Jackson, Debha Amatya
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: monomer 1
sequence annotation: monomer 2
sequence annotation: monomer 3
sequence annotation: monomer 4
sequence annotation: monomer 5
sequence annotation: monomer 6
sequence: https://synbiohub.org/public/igem/BBa_M36500_sequence/1

monomer 1
type: SequenceAnnotation
persistent identity: annotation2166600
display id: annotation2166600
version: 1
top level: spilk 7.0.
location: https://synbiohub.org/public/igem/BBa_M36500/annotation2166600/range2166600/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M36500/annotation2166600/range2166600/1
type: Range
persistent identity: range2166600
display id: range2166600
version: 1
top level: spilk 7.0.
direction: direction/forward
start: 1
end: 105
orientation: inline

monomer 2
type: SequenceAnnotation
persistent identity: annotation2166601
display id: annotation2166601
version: 1
top level: spilk 7.0.
location: https://synbiohub.org/public/igem/BBa_M36500/annotation2166601/range2166601/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M36500/annotation2166601/range2166601/1
type: Range
persistent identity: range2166601
display id: range2166601
version: 1
top level: spilk 7.0.
direction: direction/forward
start: 106
end: 210
orientation: inline

monomer 3
type: SequenceAnnotation
persistent identity: annotation2166602
display id: annotation2166602
version: 1
top level: spilk 7.0.
location: https://synbiohub.org/public/igem/BBa_M36500/annotation2166602/range2166602/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_M36500/annotation2166602/range2166602/1
type: Range
persistent identity: range2166602
display id: range2166602
version: 1
top level: spilk 7.0.
direction: direction/forward
start: 211
end: 315
orientation: inline

monomer 4
type: SequenceAnnotation
persistent identity: annotation2166603
display id: annotation2166603
version: 1
top level: spilk 7.0.
location: https://synbiohub.org/public/igem/BBa_M36500/annotation2166603/range2166603/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M36500/annotation2166603/range2166603/1
type: Range
persistent identity: range2166603
display id: range2166603
version: 1
top level: spilk 7.0.
direction: direction/forward
start: 316
end: 420
orientation: inline

monomer 5
type: SequenceAnnotation
persistent identity: annotation2166604
display id: annotation2166604
version: 1
top level: spilk 7.0.
location: https://synbiohub.org/public/igem/BBa_M36500/annotation2166604/range2166604/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M36500/annotation2166604/range2166604/1
type: Range
persistent identity: range2166604
display id: range2166604
version: 1
top level: spilk 7.0.
direction: direction/forward
start: 421
end: 525
orientation: inline

monomer 6
type: SequenceAnnotation
persistent identity: annotation2166605
display id: annotation2166605
version: 1
top level: spilk 7.0.
location: https://synbiohub.org/public/igem/BBa_M36500/annotation2166605/range2166605/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M36500/annotation2166605/range2166605/1
type: Range
persistent identity: range2166605
display id: range2166605
version: 1
top level: spilk 7.0.
direction: direction/forward
start: 526
end: 630
orientation: inline

Fos
type: ComponentDefinition
persistent identity: BBa M36501
display id: BBa_M36501
version: 1
was generated by: iGEM to SBOL conversion
description: Fos-p1n
created: 2011-12-03T12:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print.
owned by: james
owned by: myers
mutable description: Fos-p1n is a protein domain that contains a leucine zipper that covalently links to Jun-p1n. Fos-p1n is useful in creating heterodimerized parts that self-assemble into tightly linked chains.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11084
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Design was modified to codon optimized for expression in E. coli bacterium.
star: false
creator: Dominique Dabija, Christopher Jackson, Debha Amatya
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_M36501_sequence/1

Jun
type: ComponentDefinition
persistent identity: BBa M36502
display id: BBa_M36502
version: 1
was generated by: iGEM to SBOL conversion
description: Jun-p1n
created: 2011-12-03T12:00:00Z
modified: 2015-05-08T01:14:05Z
mutable provenance: O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print.
owned by: james
owned by: myers
mutable description: Jun-p1n is a protein domain that contains a leucine zipper that covalently bonds to Fos-p1n. Jun-p1n is useful for creating heterodimerized proteins that self-assemble into tightly linked chains.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11084
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part was codon optimized for expression in the E. coli bacterium.
star: false
creator: Dominique Dabija, Christopher Jackson, Debha Amatya
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_M36502_sequence/1

BBa_M36292
type: ComponentDefinition
persistent identity: BBa M36292
display id: BBa_M36292
version: 1
was generated by: iGEM to SBOL conversion
description: Transcription Terminator (99% efficient)
created: 2011-12-06T12:00:00Z
modified: 2015-05-08T01:14:04Z
mutable provenance: The part comes from the E. Coli chassis.
owned by: james
owned by: myers
mutable description: This is a terminator sequence to stop transcription. Put it at the very end of your sequence.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11078
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence is originally from BIOFAB.
star: false
creator: Dominique Dabija, Christopher Jackson, Debha Amatya
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_M36292_sequence/1

BCRBS
type: ComponentDefinition
persistent identity: BBa M36293
display id: BBa_M36293
version: 1
was generated by: iGEM to SBOL conversion
description: 5' Bicistronic UTR, includes ATG start
created: 2011-12-06T12:00:00Z
modified: 2015-05-08T01:14:04Z
mutable provenance: This part is from the E. Coli chassis.
owned by: james
owned by: myers
mutable description: This is a bicistonic sequence which includes a ribosome binding site, a leader, another ribosome binding site, and the start codon ATG. This goes at the beginning of the actuator to help the ribosome bind and start translation.
discontinued: false
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11078
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This sequence is originally from BIOFAB. It is part BD12, apFAB535.
star: false
creator: Dominique Dabija, Christopher Jackson, Debha Amatya
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_M36293_sequence/1

halt
type: ComponentDefinition
persistent identity: BBa M36296
display id: BBa_M36296
version: 1
was generated by: iGEM to SBOL conversion
description: Stop Codon TAA
created: 2011-12-06T12:00:00Z
modified: 2015-05-08T01:14:04Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: This is a stop codon for E.coli. The sequence is TAA
discontinued: true
dominant: false
experience: experience/None
group u list: _848_
m user id: 0
owner id: 11065
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Codon optimized for E.coli
star: false
creator: Christopher Jackson
type: DnaRegion
role: partType/Protein Domain
role: SO:0000417
sequence: https://synbiohub.org/public/igem/BBa_M36296_sequence/1

BBa_M36394
type: ComponentDefinition
persistent identity: BBa M36394
display id: BBa_M36394
version: 1
was generated by: iGEM to SBOL conversion
description: Spider Silk Actuator
created: 2011-12-06T12:00:00Z
modified: 2015-05-08T01:14:04Z
mutable provenance: RBS Leader and ATG came from BioFAB. Terminator comes from BioFAB. Spider silk monomer sequence was repeated six times, and the monomer AA sequence comes from Xia, X.-X., Z.-G. Qian, C. S. Ki, Y. H. Park, D. L. Kaplan, and S. Y. Lee. "Native-sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber." Proceedings of the National Academy of Sciences 107.32 (2010): 14059-4063. Print. The sequence was codon optimized for E. coli. Fos-Jun leucine zipper sequences were taken from . O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print. They were also codon optimized for E.coli. Our system was placed into a Rhamnex 67K Xbrane plasmid and tested using varying concentrations of rhamnose.
owned by: james
owned by: myers
mutable description: This is a Spider Silk Generating Device that uses Fos and Jun leucine zipper sequences flanking a spider silk protein hexamer. The leucine zippers are linked to the spider silk hexamer by 8 AA linkers. RBS+Leader+ATG are part of the start of the gene, and the terminator is at the end.
discontinued: false
dominant: false
experience: experience/Works
group u list: _848_
m user id: 0
owner id: 11065
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: Our overall design had a base pair limit of 3000. This pointed to usage of Fos and Jun leucine zippers to attempt to get the spider silk hexamers to link up head to tail via covalent bonds in the E. coli cells. We were not able to spin the spider silk protein out, isolate it, or analyze its structural properties.
star: false
creator: Dominique Dabija, Christopher Jackson, Debha Amatya
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M36293
component: BBa_K133132
component: BBa_M36501
component: BBa_M36500
component: BBa_M36292
component: BBa_M36502
sequence annotation: BBa_M36293
sequence annotation: BBa_M36501
sequence annotation: BBa_K133132
sequence annotation: BBa_M36500
sequence annotation: BBa_M36502
sequence annotation: BBa_M36292
sequence: https://synbiohub.org/public/igem/BBa_M36394_sequence/1

BBa_M36293
type: Component
persistent identity: component2166606
display id: component2166606
version: 1
top level: BBa_M36394
definition: BCRBS
access: public

BBa_K133132
type: Component
persistent identity: component2166608
display id: component2166608
version: 1
top level: BBa_M36394
definition: linker
access: public

BBa_M36501
type: Component
persistent identity: component2166607
display id: component2166607
version: 1
top level: BBa_M36394
definition: Fos
access: public

BBa_K133132
type: Component
persistent identity: component2166616
display id: component2166616
version: 1
top level: BBa_M36394
definition: linker
access: public

BBa_M36500
type: Component
persistent identity: component2166615
display id: component2166615
version: 1
top level: BBa_M36394
definition: spilk 7.0.
access: public

BBa_M36292
type: Component
persistent identity: component2166618
display id: component2166618
version: 1
top level: BBa_M36394
definition: BBa_M36292
access: public

BBa_M36502
type: Component
persistent identity: component2166617
display id: component2166617
version: 1
top level: BBa_M36394
definition: Jun
access: public

BBa_M36293
type: SequenceAnnotation
persistent identity: annotation2166606
display id: annotation2166606
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166606/range2166606/1
component: BBa_M36293

https://synbiohub.org/public/igem/BBa_M36394/annotation2166606/range2166606/1
type: Range
persistent identity: range2166606
display id: range2166606
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 1
end: 88
orientation: inline

BBa_M36501
type: SequenceAnnotation
persistent identity: annotation2166607
display id: annotation2166607
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166607/range2166607/1
component: BBa_M36501

https://synbiohub.org/public/igem/BBa_M36394/annotation2166607/range2166607/1
type: Range
persistent identity: range2166607
display id: range2166607
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 97
end: 216
orientation: inline

BBa_K133132
type: SequenceAnnotation
persistent identity: annotation2166608
display id: annotation2166608
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166608/range2166608/1
component: BBa_K133132

https://synbiohub.org/public/igem/BBa_M36394/annotation2166608/range2166608/1
type: Range
persistent identity: range2166608
display id: range2166608
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 225
end: 248
orientation: inline

BBa_M36500
type: SequenceAnnotation
persistent identity: annotation2166615
display id: annotation2166615
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166615/range2166615/1
component: BBa_M36500

https://synbiohub.org/public/igem/BBa_M36394/annotation2166615/range2166615/1
type: Range
persistent identity: range2166615
display id: range2166615
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 257
end: 886
orientation: inline

BBa_K133132
type: SequenceAnnotation
persistent identity: annotation2166616
display id: annotation2166616
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166616/range2166616/1
component: BBa_K133132

https://synbiohub.org/public/igem/BBa_M36394/annotation2166616/range2166616/1
type: Range
persistent identity: range2166616
display id: range2166616
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 895
end: 918
orientation: inline

BBa_M36502
type: SequenceAnnotation
persistent identity: annotation2166617
display id: annotation2166617
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166617/range2166617/1
component: BBa_M36502

https://synbiohub.org/public/igem/BBa_M36394/annotation2166617/range2166617/1
type: Range
persistent identity: range2166617
display id: range2166617
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 927
end: 1046
orientation: inline

BBa_M36292
type: SequenceAnnotation
persistent identity: annotation2166618
display id: annotation2166618
version: 1
top level: BBa_M36394
location: https://synbiohub.org/public/igem/BBa_M36394/annotation2166618/range2166618/1
component: BBa_M36292

https://synbiohub.org/public/igem/BBa_M36394/annotation2166618/range2166618/1
type: Range
persistent identity: range2166618
display id: range2166618
version: 1
top level: BBa_M36394
direction: direction/reverse
start: 1055
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linker
type: ComponentDefinition
persistent identity: BBa K133132
display id: BBa_K133132
version: 1
was generated by: iGEM to SBOL conversion
description: 8 aa protein domain linker
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:09:57Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/None
group u list: _231_
m user id: 0
owner id: 3358
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Jan Lonzaric
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence: https://synbiohub.org/public/igem/BBa_K133132_sequence/1

https://synbiohub.org/public/igem/BBa_M36294_sequence/1
type: Sequence
persistent identity: BBa M36294 sequence
display id: BBa_M36294_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatgtactagagtaatactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatgtactagagctgaccgataccctgcaggcagaaaccgatcagctggaagataaaaaaagcgcactgcagaccgaaattgcaaatctgctgaaagaaaaagaaaaactggaatttattctggcagcatattactagagtccgcttgttactgtgagctttcctactagagtcaggacgggggggacttggagggcaaggagcggggatggcggcggcggccgcaatggggggagcgggacaggggggatacggagggttaggatcacaaggaacctctggaagaggaggattgggaggacagggggcagggatggcggcagctgctgctatgggcggtgctggccaaggtgggtacgggggtttagggtcccaagggacgtctgggagaggagggttgggtgggcaaggggctgggatggctgctgcagcggctatggggggcgctggacaagggggctatgggggacttgggtcccaaggaacgagcggtcggggcgggcttggggggcaaggggccggaatggcagcggctgccgcgatgggaggtgcgggacaaggagggtacggggggcttgggtctcaaggtactagcggccgggggggattaggtggccaaggagctggaatggccgcggctgcggccatgggcggagcaggtcaaggtggctatggcggattgggaagtcagggcacgtccggacgcggtggcctagggggacaaggtgcaggaatggcggctgctgctgccatgggaggggcgggccagggcggatacggtggccttgggtcacaaggtacctactagagtccgcttgttactgtgagctttcctactagagcgtattgcacgtctggaagaaaaagttaaaaccctgaaagcacagaatagcgaactggcaagcaccgcaaatatgctgcgtgaacaggttgcacagctgaaacagaaagttatgaattattactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtctactagagtcaggacgggggggacttggagggcaaggagcggggatggcggcggcggccgcaatggggggagcgggacaggggggatacggagggttaggatcacaaggaacctctggaagaggaggattgggaggacagggggcagggatggcggcagctgctgctatgggcggtgctggccaaggtgggtacgggggtttagggtcccaagggacgtctgggagaggagggttgggtgggcaaggggctgggatggctgctgcagcggctatggggggcgctggacaagggggctatgggggacttgggtcccaaggaacgagcggtcggggcgggcttggggggcaaggggccggaatggcagcggctgccgcgatgggaggtgcgggacaaggagggtacggggggcttgggtctcaaggtactagcggccgggggggattaggtggccaaggagctggaatggccgcggctgcggccatgggcggagcaggtcaaggtggctatggcggattgggaagtcagggcacgtccggacgcggtggcctagggggacaaggtgcaggaatggcggctgctgctgccatgggaggggcgggccagggcggatacggtggccttgggtcacaaggtacctactagagctgaccgataccctgcaggca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36500_sequence/1
type: Sequence
persistent identity: BBa M36500 sequence
display id: BBa_M36500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcaggacgggggggacttggagggcaaggagcggggatggcggcggcggccgcaatggggggagcgggacaggggggatacggagggttaggatcacaaggaacctctggaagaggaggattgggaggacagggggcagggatggcggcagctgctgctatgggcggtgctggccaaggtgggtacgggggtttagggtcccaagggacgtctgggagaggagggttgggtgggcaaggggctgggatggctgctgcagcggctatggggggcgctggacaagggggctatgggggacttgggtcccaaggaacgagcggtcggggcgggcttggggggcaaggggccggaatggcagcggctgccgcgatgggaggtgcgggacaaggagggtacggggggcttgggtctcaaggtactagcggccgggggggattaggtggccaaggagctggaatggccgcggctgcggccatgggcggagcaggtcaaggtggctatggcggattgggaagtcagggcacgtccggacgcggtggcctagggggacaaggtgcaggaatggcggctgctgctgccatgggaggggcgggccagggcggatacggtggccttgggtcacaaggtacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36501_sequence/1
type: Sequence
persistent identity: BBa M36501 sequence
display id: BBa_M36501_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgaccgataccctgcaggcagaaaccgatcagctggaagataaaaaaagcgcactgcagaccgaaattgcaaatctgctgaaagaaaaagaaaaactggaatttattctggcagcatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36502_sequence/1
type: Sequence
persistent identity: BBa M36502 sequence
display id: BBa_M36502_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtattgcacgtctggaagaaaaagttaaaaccctgaaagcacagaatagcgaactggcaagcaccgcaaatatgctgcgtgaacaggttgcacagctgaaacagaaagttatgaattat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36292_sequence/1
type: Sequence
persistent identity: BBa M36292 sequence
display id: BBa_M36292_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36293_sequence/1
type: Sequence
persistent identity: BBa M36293 sequence
display id: BBa_M36293_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36296_sequence/1
type: Sequence
persistent identity: BBa M36296 sequence
display id: BBa_M36296_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M36394_sequence/1
type: Sequence
persistent identity: BBa M36394 sequence
display id: BBa_M36394_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatgtactagagctgaccgataccctgcaggcagaaaccgatcagctggaagataaaaaaagcgcactgcagaccgaaattgcaaatctgctgaaagaaaaagaaaaactggaatttattctggcagcatattactagagtccgcttgttactgtgagctttcctactagagtcaggacgggggggacttggagggcaaggagcggggatggcggcggcggccgcaatggggggagcgggacaggggggatacggagggttaggatcacaaggaacctctggaagaggaggattgggaggacagggggcagggatggcggcagctgctgctatgggcggtgctggccaaggtgggtacgggggtttagggtcccaagggacgtctgggagaggagggttgggtgggcaaggggctgggatggctgctgcagcggctatggggggcgctggacaagggggctatgggggacttgggtcccaaggaacgagcggtcggggcgggcttggggggcaaggggccggaatggcagcggctgccgcgatgggaggtgcgggacaaggagggtacggggggcttgggtctcaaggtactagcggccgggggggattaggtggccaaggagctggaatggccgcggctgcggccatgggcggagcaggtcaaggtggctatggcggattgggaagtcagggcacgtccggacgcggtggcctagggggacaaggtgcaggaatggcggctgctgctgccatgggaggggcgggccagggcggatacggtggccttgggtcacaaggtacctactagagtccgcttgttactgtgagctttcctactagagcgtattgcacgtctggaagaaaaagttaaaaccctgaaagcacagaatagcgaactggcaagcaccgcaaatatgctgcgtgaacaggttgcacagctgaaacagaaagttatgaattattactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K133132_sequence/1
type: Sequence
persistent identity: BBa K133132 sequence
display id: BBa_K133132_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccgcttgttactgtgagctttcc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z