BBa_M36306 1 BBa_M36306 FseI restriction endonuclease 2011-12-08T12:00:00Z 2015-05-08T01:14:04Z Frankia species Eul1b (NRRL 18528) The coding sequence for the FseI restriction endonuclease codon optimized for use in E. coli. The enzyme will cleave DNA at sites with the sequence 5'...GGCCGG|CC...3'. The cutting position is indicated by '|' Used to cut specific DNA sequences that FseI recognizes. false false _848_ 0 11095 9 Not in stock false Codon optimization for use with E. coli false Raman Nelakanti, Dennis Te, Darren Jindal BBa_M36282 1 BBa_M36282 5' Bicistronic UTR, includes ATG start codon 2011-11-29T12:00:00Z 2015-05-08T01:14:04Z BioFab: apFAB563 This sequence contains an RBS followed by the sequence TAATG. The parts number is BBa_M36000 (PartsRegistry). The first RBS sequence is strong, while the second one is weak. This stops the first polymerase with the stop codon TAA, but allows for a second polymerase to bind to the start codon ATG(BIOFAB). This sequence prevents a hairpin loop from being formed with another part of the sequence, which would decrease the amount of translation. The bicistronic sequence ends with a start codon ATG. false true _848_ 0 11068 9 Not in stock false no false Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso annotation2166677 1 Start Codon range2166677 1 86 88 BBa_M36307 1 BBa_M36307 FseI restriction endonuclease actuator construct 2011-12-08T12:00:00Z 2015-05-08T01:14:04Z Multiple: E. coli, Frankia, and coliphageT7 respectively. Enter description here: false false _848_ 0 11095 9 Not in stock false Codon optimized for E. coli. false Raman Nelakanti, Darren Jindal, Dennis Te component2166597 1 BBa_M36306 component2166596 1 BBa_M36282 component2166598 1 BBa_M36305 annotation2166598 1 BBa_M36305 range2166598 1 762 809 annotation2166596 1 BBa_M36282 range2166596 1 1 88 annotation2166597 1 BBa_M36306 range2166597 1 97 753 BBa_M36305 1 BBa_M36305 Transcription terminator pT-T7 from bacteriophage 2011-12-08T12:00:00Z 2015-05-08T01:14:04Z T7 Bacteriophage Released HQ 2013 A transcription terminator derived from T7 bacteriophage. Use where transcription should be stopped for the gene of interest upstream of this terminator. false false _848_ 0 11095 9 In stock false None. false Darren Jindal, Raman Nelakanti, Dennis Te BBa_M36307_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagagaccgatgagctgttccctattccggaaccactggtacgtccagtgatcgccctgccgccccacttaaaagagctgatcgacctgctgccgctgaacactccggttcaccgccgtgatctggaagctaaatatggtcgttccaactacgctcgtcgtattcgtaagatcatcagtgagtacggctgggaaatcgaaagccgtcgtcaaagcgaaggcgcgaacgacgattggtatatccgtcgttctgatggtccggttcgccctcagcgcatccgtcgtgaagttccgcgtcgcagtcgtgaaactgtgtaccgccgtgacgactggatttgtcagatctgtcgtatgaaaactgacccagagcgtggttccctggtaccgcaatgcgaccacaaaatcccggcagaccgcggtggcgactctgacgaagaaaatctgcagaccctctgcacccgttgcaacctgaaaaaacgtcaggcttgtggtggttgtgcgctggctagctgcgcggattgcccgttcgcatacccggaaaaattcgatgacgttctgatcctgcatctggaccgcgaacatctgaaacgtattatgaccaccgcatatgctcgtaacgttactgccagcgctgtcgtgtccgatctgagcgacctgctgtagtactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_M36282_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatg BBa_M36305_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_M36306_sequence 1 accgatgagctgttccctattccggaaccactggtacgtccagtgatcgccctgccgccccacttaaaagagctgatcgacctgctgccgctgaacactccggttcaccgccgtgatctggaagctaaatatggtcgttccaactacgctcgtcgtattcgtaagatcatcagtgagtacggctgggaaatcgaaagccgtcgtcaaagcgaaggcgcgaacgacgattggtatatccgtcgttctgatggtccggttcgccctcagcgcatccgtcgtgaagttccgcgtcgcagtcgtgaaactgtgtaccgccgtgacgactggatttgtcagatctgtcgtatgaaaactgacccagagcgtggttccctggtaccgcaatgcgaccacaaaatcccggcagaccgcggtggcgactctgacgaagaaaatctgcagaccctctgcacccgttgcaacctgaaaaaacgtcaggcttgtggtggttgtgcgctggctagctgcgcggattgcccgttcgcatacccggaaaaattcgatgacgttctgatcctgcatctggaccgcgaacatctgaaacgtattatgaccaccgcatatgctcgtaacgttactgccagcgctgtcgtgtccgatctgagcgacctgctgtag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z