BBa_M36344 1 BBa_M36344 Actuator for Grape Scent 2012-12-04T12:00:00Z 2015-05-08T01:14:04Z The amino acid sequence is from the UniProt database originating from the below paper. Wang, J., De Luca, V. ???The biosynthesis and regulation of biosynthesis of Concord grape fruit esters, including ???foxy??? methylanthranilate.??? The Plant Journal. 2005. Vol. 44, pp. 606-619. Our gene produces the AMAT enzyme (Anthraniloyl-coenzymeA: methanol acyltransferase), which catalyzes the reaction between anthraniloyl coA and methanol to produce methyl anthranilate. false false _848_ 0 15027 9 Not in stock false We obtained the original amino acid sequence and used OPTIMIZER, referenced below, to arrive at the desired nucleotide sequence. A base pair had to be modified from this codon translation to avoid a palindrome in the nucleotide sequence. Specifically, base pair 1078 of our sequence was changed from a G to a C. Puigb??, P., Guzm??n, E., Romeu A., and Garcia-Vallv?? S. ???OPTIMIZER: a web server for optimizing the codon usage of DNA sequences.??? Nucleic Acids Res. July, 2007. Issue 35. false Chris Aholt, Arjun Bhargava, Nikhil Bhargava annotation2251379 1 polya range2251379 1 1894 1907 annotation2251369 1 stem_loop range2251369 1 1653 1696 annotation2251363 1 BBa_M36282 range2251363 1 193 280 annotation2251362 1 Start Codon range2251362 1 182 184 annotation2251364 1 Start Codon range2251364 1 278 280 annotation2251368 1 BBa_B0010 range2251368 1 1642 1721 annotation2251378 1 T7 TE range2251378 1 1874 1893 annotation2251377 1 BBa_B0012 range2251377 1 1867 1907 annotation2251366 1 point mutation to avoid palindrome range2251366 1 1270 1270 annotation2251384 1 T7 TE range2251384 1 2011 2030 annotation2251376 1 stem_loop range2251376 1 1790 1833 annotation2251367 1 BBa_B0015 range2251367 1 1642 1770 annotation2251360 1 BBa_M36282 range2251360 1 1 88 annotation2251383 1 BBa_B0012 range2251383 1 2004 2044 annotation2251373 1 stop range2251373 1 1763 1763 annotation2251381 1 BBa_B0010 range2251381 1 1916 1995 annotation2251372 1 polya range2251372 1 1757 1770 annotation2251387 1 BBa_B0015 range2251387 1 1916 2044 annotation2251359 1 Start Codon range2251359 1 86 88 annotation2251371 1 T7 TE range2251371 1 1737 1756 annotation2251385 1 polya range2251385 1 2031 2044 annotation2251380 1 stop range2251380 1 1900 1900 annotation2251370 1 BBa_B0012 range2251370 1 1730 1770 annotation2251374 1 BBa_B0010 range2251374 1 1779 1858 annotation2251375 1 BBa_B0015 range2251375 1 1779 1907 annotation2251386 1 stop range2251386 1 2037 2037 annotation2251361 1 BBa_M36282 range2251361 1 97 184 annotation2251382 1 stem_loop range2251382 1 1927 1970 annotation2251365 1 BBa_M36344 range2251365 1 287 1633 BBa_M36344_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagaggggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctaaggaggttttctaatgtactagatggcgtctccgtcttctccgctggttttctctgttaaccgttgcgttccgcagatcgttcgtccggcgaacccgaccccgcgtgaagttaaacagctgtctgacatcgacgaccaggaaggtcgtcgtttccagatcccggttatcatgttctaccgtaacaacccgctgatggaaggtaaagacccggttaaagttatccgtgaagcgctgggtaaagcgctggtttactactacccgttcgcgggtcgtctgatcgaaggtgacaaccgtaaactgatggttgactgcaccggtgaaggtgttctgttcatcgaagcggacgcggacaccaccctggaaaacctgggtgacgcgatccagccgatgtgcccgtgcttcgaagaactgctgtacgacgttccgggttctaccaccatcctgggttctccgctgatcctgatccaggttacccgtctgcgttgcggtggtttcatcttcgcgctgcgtctgaaccacaccatgtctgacgcggcgggtctgatccagttcctggacaccatcggtgaaatggcgcagggtctgtctgttccgtctctgctgccgatctggcagcgtgaactgctgaacgcgcgtaacccgccgcgtatcacccgtatccaccacgaatacgaaaaagttaccaacaccaaaggtaccctgatggcgatggacgaaaactctctggttcaccgttctttcttcttcggtcgtgaagaaatccgtgcgctgcgtaaccgtctgccggcgtctctgggtgcgtgctctaccttcgaagttctgatggcgtgcgtttggcgttgccgtaccatcgcgttcgcggttgacccggacgaagttgttcgtatctcttgcatcatcaacatgcgtggtaaacacggtttcgaactgccgccgggttactacggtaacgcgttcgttaccccggcgtctatcaccaaagcgggtatgctgtgcaaaaacccgctggaattcgcgatccgtctggttaaaaaagcgaaagcggaaatgtctcaggaatacatcaaatctgttgcggacctgatggttatcaaaggtcgtccgctgttcacccagccgggtaacttcaccgtttctgacgttacccgtgcgggtctgggtgaagttgacttcggttggggtaaacctgtttacggtggtgttgcgcgtgcgtgcccgatcatctctttccgtatgctgttccgtaactctaaaggtgaagaaggttctgttatcccgatctggctgccgccgccggttatggaacgtttcgaacaggaactgaaacgtatgaccaaaaaagcggaactgctgatcacctctatgctgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z