BBa_M36291 1 BBa_M36291 Transcription terminator (apFAB391) (99% efficient) 2011-12-09T12:00:00Z 2015-05-08T01:14:04Z BIOFAB.org This part is a transcription terminator. false false _848_ 0 11080 9 Not in stock false none. false Victoria Robles, Kyung Ri Park, Ken Xiong BBa_M36363 1 BBa_M36363 PoPS -> DbjA 2013-10-24T11:00:00Z 2015-05-08T01:14:04Z DbjA haloalkane dehalogenase is from the Bradyrhizobium japonicum USDA110 genome. The 5' BCD UTR and terminator are both from the BIOFAB database. This composite part is an actuator that converts PoPS into production of the DbjA haloalkane dehalogenase. This device has a low strength 5' BCD UTR and a strong terminator. false false _848_ 0 19036 9 Not in stock false The 5' BCD UTR was not originally intended to be weak, but an error in selection led to the use of the incorrect BCD part. false Will Connors, Ethan Li, and Shirbi Ish-Shalom component2370621 1 BBa_M36361 component2370622 1 BBa_M36362 component2370623 1 BBa_M36291 annotation2370623 1 BBa_M36291 range2370623 1 1031 1112 annotation2370621 1 BBa_M36361 range2370621 1 1 83 annotation2370622 1 BBa_M36362 range2370622 1 90 1022 BBa_M36361 1 BBa_M36361 5' Bicistronic UTR (apFAB866, GENE10_BCD2), No ATG Start 2013-10-24T11:00:00Z 2015-05-08T01:14:04Z This part was sourced from the Supplemental Table 1 of Mutalik, et al., where it was constructed as a candidate sequence-independent BCD. 5' UTR based on bicistronic junction architecture, composed of a GENE10 SD1 context and a BCD2 SD2 region. Has low strength. false false _848_ 0 19036 9 Not in stock false This sequence was copied verbatim. false Ethan Li BBa_M36362 1 BBa_M36362 DbjA Haloalkane Dehalogenase 2013-10-24T11:00:00Z 2015-05-08T01:14:04Z This part was sourced from the genomic sequence of the soil bacterium Bradyrhizobium japonicum USDA110, at Accession NP_767727 and Gene ID 27376198. No mutations were made. This part encodes an enzyme with dehalogenase activity for haloalkane substrates. DbjA catalyzes the cleavage of carbon-halogen bonds to form a primary alcohol and a halide. DbjA generally has higher overall activity than other haloalkane dehalogenases. Unlike most other known haloalkane dehalogenases, DbjA has very high activity on 2-bromo-1-chloropropane and chlorocyclopentane, and it has significant activity towards 1,2-dichloropropane (http://www.biochemj.org/bj/435/0345/bj4350345.htm). ATG and TAA stop codons are included, so this part can just be flanked by a 5' UTR translation initiator and a 3' transcription terminator for use. false false _848_ 0 19036 9 Not in stock false The amino acid sequence was backtranslated through Gene Designer's genetic algorithm (Maximum Generations: 100; Maximum Mutation Rate: 0.05; Minimum Best Individual Selection Rate: 0.9; Population Size: 50; Number of Progeny: 25) into a codon set optimized for Escherichia coli K12. This algorithm was given additional parameters to avoid BbsI, BsmBI, and BsaI restriction sites (at a weight of 1), hairpins (at a weight of 1), and repeats of at least 10 bp (at a weight of 1). A TAA stop codon was then added at the end of the resulting DNA sequence. false Will Connors, Ethan Li, and Shirbi Ish-Shalom BBa_M36361_sequence 1 aataattttgtttaactttaagaaggaggtatatccatggctagcatgactaaacatcttaatcatgctaaggaggttttcta BBa_M36362_sequence 1 atgtctaaaccgattgaaattgagattcggcgtgctccggtcttgggcagctcgatggcctatcgtgagactggtgcacaggatgctcccgttgtgctgttcctccacggcaatccgacctctagccatatttggcgcaacatcctgccactggttagtcccgttgctcactgcattgcgccggatctgattggtttcggccaatccggtaaaccggatattgcgtaccgctttttcgaccatgtccgttatttagacgcctttatcgaacagcgcggcgttacatcagcatatctggtggcgcaggactggggtaccgcgctggcgtttcatctggcagcgcgccgccccgactttgttcgtggactcgcctttatggaattcattcgcccgatgccgacgtggcaggacttccaccatacagaagtggccgaggaacaggatcacgcggaagctgcccgtgctgtgtttcgaaaattccgcacgccgggagagggagaagcaatgattcttgaagcaaacgccttcgtcgaaagggtgttaccgggcggcatcgtacgcaaactgggtgacgaggaaatggccccatatcgtactccgttcccgaccccggagagccgccgaccggtgttagcctttccacgtgagttgccgatagctggcgaaccggctgacgtttacgaggctcttcagtcggcgcatgcggccttggcggcgagttcttaccccaaactgttatttaccggagaaccaggcgccctggtgtcgccggaatttgcggagcgttttgcagcgtcgttaactcgttgcgcattgattcgccttggcgcgggcctgcattacctgcaagaggatcatgctgatgcgataggccgctccgtggcgggctggattgccggcattgaagccgtccgcccgcagcttgccgcgtaa BBa_M36291_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36363_sequence 1 aataattttgtttaactttaagaaggaggtatatccatggctagcatgactaaacatcttaatcatgctaaggaggttttctatactagatgtctaaaccgattgaaattgagattcggcgtgctccggtcttgggcagctcgatggcctatcgtgagactggtgcacaggatgctcccgttgtgctgttcctccacggcaatccgacctctagccatatttggcgcaacatcctgccactggttagtcccgttgctcactgcattgcgccggatctgattggtttcggccaatccggtaaaccggatattgcgtaccgctttttcgaccatgtccgttatttagacgcctttatcgaacagcgcggcgttacatcagcatatctggtggcgcaggactggggtaccgcgctggcgtttcatctggcagcgcgccgccccgactttgttcgtggactcgcctttatggaattcattcgcccgatgccgacgtggcaggacttccaccatacagaagtggccgaggaacaggatcacgcggaagctgcccgtgctgtgtttcgaaaattccgcacgccgggagagggagaagcaatgattcttgaagcaaacgccttcgtcgaaagggtgttaccgggcggcatcgtacgcaaactgggtgacgaggaaatggccccatatcgtactccgttcccgaccccggagagccgccgaccggtgttagcctttccacgtgagttgccgatagctggcgaaccggctgacgtttacgaggctcttcagtcggcgcatgcggccttggcggcgagttcttaccccaaactgttatttaccggagaaccaggcgccctggtgtcgccggaatttgcggagcgttttgcagcgtcgttaactcgttgcgcattgattcgccttggcgcgggcctgcattacctgcaagaggatcatgctgatgcgataggccgctccgtggcgggctggattgccggcattgaagccgtccgcccgcagcttgccgcgtaatactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z