BBa_M36292 1 BBa_M36292 Transcription Terminator (99% efficient) 2011-12-06T12:00:00Z 2015-05-08T01:14:04Z The part comes from the E. Coli chassis. This is a terminator sequence to stop transcription. Put it at the very end of your sequence. false false _848_ 0 11078 9 Not in stock false The sequence is originally from BIOFAB. false Dominique Dabija, Christopher Jackson, Debha Amatya BBa_M36394 1 BBa_M36394 Spider Silk Actuator 2011-12-06T12:00:00Z 2015-05-08T01:14:04Z RBS Leader and ATG came from BioFAB. Terminator comes from BioFAB. Spider silk monomer sequence was repeated six times, and the monomer AA sequence comes from Xia, X.-X., Z.-G. Qian, C. S. Ki, Y. H. Park, D. L. Kaplan, and S. Y. Lee. "Native-sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber." Proceedings of the National Academy of Sciences 107.32 (2010): 14059-4063. Print. The sequence was codon optimized for E. coli. Fos-Jun leucine zipper sequences were taken from . O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print. They were also codon optimized for E.coli. Our system was placed into a Rhamnex 67K Xbrane plasmid and tested using varying concentrations of rhamnose. This is a Spider Silk Generating Device that uses Fos and Jun leucine zipper sequences flanking a spider silk protein hexamer. The leucine zippers are linked to the spider silk hexamer by 8 AA linkers. RBS+Leader+ATG are part of the start of the gene, and the terminator is at the end. false false _848_ 0 11065 9 Not in stock true Our overall design had a base pair limit of 3000. This pointed to usage of Fos and Jun leucine zippers to attempt to get the spider silk hexamers to link up head to tail via covalent bonds in the E. coli cells. We were not able to spin the spider silk protein out, isolate it, or analyze its structural properties. false Dominique Dabija, Christopher Jackson, Debha Amatya component2166617 1 BBa_M36502 component2166616 1 BBa_K133132 component2166607 1 BBa_M36501 component2166608 1 BBa_K133132 component2166615 1 BBa_M36500 component2166618 1 BBa_M36292 component2166606 1 BBa_M36293 annotation2166616 1 BBa_K133132 range2166616 1 895 918 annotation2166617 1 BBa_M36502 range2166617 1 927 1046 annotation2166618 1 BBa_M36292 range2166618 1 1055 1136 annotation2166607 1 BBa_M36501 range2166607 1 97 216 annotation2166615 1 BBa_M36500 range2166615 1 257 886 annotation2166608 1 BBa_K133132 range2166608 1 225 248 annotation2166606 1 BBa_M36293 range2166606 1 1 88 BBa_K133132 1 linker 8 aa protein domain linker 2008-10-23T11:00:00Z 2015-05-08T01:09:57Z false false _231_ 0 3358 9 It's complicated false false Jan Lonzaric BBa_M36293 1 BCRBS 5' Bicistronic UTR, includes ATG start 2011-12-06T12:00:00Z 2015-05-08T01:14:04Z This part is from the E. Coli chassis. This is a bicistonic sequence which includes a ribosome binding site, a leader, another ribosome binding site, and the start codon ATG. This goes at the beginning of the actuator to help the ribosome bind and start translation. false false _848_ 0 11078 9 Not in stock false This sequence is originally from BIOFAB. It is part BD12, apFAB535. false Dominique Dabija, Christopher Jackson, Debha Amatya BBa_M36502 1 Jun Jun-p1n 2011-12-03T12:00:00Z 2015-05-08T01:14:05Z O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print. Jun-p1n is a protein domain that contains a leucine zipper that covalently bonds to Fos-p1n. Jun-p1n is useful for creating heterodimerized proteins that self-assemble into tightly linked chains. false false _848_ 0 11084 9 Not in stock false This part was codon optimized for expression in the E. coli bacterium. false Dominique Dabija, Christopher Jackson, Debha Amatya BBa_M36501 1 Fos Fos-p1n 2011-12-03T12:00:00Z 2015-05-08T01:14:05Z O'Shea, E., R. Rutkowski, W. Stafford, and P. Kim. "Preferential Heterodimer Formation by Isolated Leucine Zippers from Fos and Jun." Science 245.4918 (1989): 646-48. Print. Fos-p1n is a protein domain that contains a leucine zipper that covalently links to Jun-p1n. Fos-p1n is useful in creating heterodimerized parts that self-assemble into tightly linked chains. false false _848_ 0 11084 9 Not in stock false Design was modified to codon optimized for expression in E. coli bacterium. false Dominique Dabija, Christopher Jackson, Debha Amatya BBa_M36500 1 spilk 7.0. Spider Silk Hexamer 2011-12-03T12:00:00Z 2015-05-08T01:14:05Z The monomer amino acid sequence was taken from Xia, X.-X., Z.-G. Qian, C. S. Ki, Y. H. Park, D. L. Kaplan, and S. Y. Lee. "Native-sized Recombinant Spider Silk Protein Produced in Metabolically Engineered Escherichia Coli Results in a Strong Fiber." Proceedings of the National Academy of Sciences 107.32 (2010): 14059-4063. Print. This is a spider silk hexamer protein. The monomer amino acid sequence was taken from Xia et al. and codon optimized for expression in E. coli. Repeats above 10 base pairs were also minimized to allow for ease of synthesis. false false _848_ 0 11065 9 Not in stock false Needed to minimize repeats and allow for ideal mRNA forming without too many loops while still maintaining codon optimization for E. coli. false Dominique Dabija, Christopher Jackson, Debha Amatya annotation2166602 1 monomer 3 range2166602 1 211 315 annotation2166604 1 monomer 5 range2166604 1 421 525 annotation2166605 1 monomer 6 range2166605 1 526 630 annotation2166603 1 monomer 4 range2166603 1 316 420 annotation2166600 1 monomer 1 range2166600 1 1 105 annotation2166601 1 monomer 2 range2166601 1 106 210 BBa_M36501_sequence 1 ctgaccgataccctgcaggcagaaaccgatcagctggaagataaaaaaagcgcactgcagaccgaaattgcaaatctgctgaaagaaaaagaaaaactggaatttattctggcagcatat BBa_M36394_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatgtactagagctgaccgataccctgcaggcagaaaccgatcagctggaagataaaaaaagcgcactgcagaccgaaattgcaaatctgctgaaagaaaaagaaaaactggaatttattctggcagcatattactagagtccgcttgttactgtgagctttcctactagagtcaggacgggggggacttggagggcaaggagcggggatggcggcggcggccgcaatggggggagcgggacaggggggatacggagggttaggatcacaaggaacctctggaagaggaggattgggaggacagggggcagggatggcggcagctgctgctatgggcggtgctggccaaggtgggtacgggggtttagggtcccaagggacgtctgggagaggagggttgggtgggcaaggggctgggatggctgctgcagcggctatggggggcgctggacaagggggctatgggggacttgggtcccaaggaacgagcggtcggggcgggcttggggggcaaggggccggaatggcagcggctgccgcgatgggaggtgcgggacaaggagggtacggggggcttgggtctcaaggtactagcggccgggggggattaggtggccaaggagctggaatggccgcggctgcggccatgggcggagcaggtcaaggtggctatggcggattgggaagtcagggcacgtccggacgcggtggcctagggggacaaggtgcaggaatggcggctgctgctgccatgggaggggcgggccagggcggatacggtggccttgggtcacaaggtacctactagagtccgcttgttactgtgagctttcctactagagcgtattgcacgtctggaagaaaaagttaaaaccctgaaagcacagaatagcgaactggcaagcaccgcaaatatgctgcgtgaacaggttgcacagctgaaacagaaagttatgaattattactagagtcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_K133132_sequence 1 tccgcttgttactgtgagctttcc BBa_M36293_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgctgcggagggtttctaatg BBa_M36292_sequence 1 tcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtc BBa_M36500_sequence 1 tcaggacgggggggacttggagggcaaggagcggggatggcggcggcggccgcaatggggggagcgggacaggggggatacggagggttaggatcacaaggaacctctggaagaggaggattgggaggacagggggcagggatggcggcagctgctgctatgggcggtgctggccaaggtgggtacgggggtttagggtcccaagggacgtctgggagaggagggttgggtgggcaaggggctgggatggctgctgcagcggctatggggggcgctggacaagggggctatgggggacttgggtcccaaggaacgagcggtcggggcgggcttggggggcaaggggccggaatggcagcggctgccgcgatgggaggtgcgggacaaggagggtacggggggcttgggtctcaaggtactagcggccgggggggattaggtggccaaggagctggaatggccgcggctgcggccatgggcggagcaggtcaaggtggctatggcggattgggaagtcagggcacgtccggacgcggtggcctagggggacaaggtgcaggaatggcggctgctgctgccatgggaggggcgggccagggcggatacggtggccttgggtcacaaggtacc BBa_M36502_sequence 1 cgtattgcacgtctggaagaaaaagttaaaaccctgaaagcacagaatagcgaactggcaagcaccgcaaatatgctgcgtgaacaggttgcacagctgaaacagaaagttatgaattat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z