BBa_M36414 1 BBa_M36414 Pb (II) Sensor 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z The promoter, RBS, and terminator regions are all standard parts of the E. coli genome. The MTF-1 and following promoter regions come from the mouse genome. The sequence was back translated from the amino acid sequence to avoid introns. This sensor will detect the lead in the form of Pb (II) within the cell environment. The promoter is constitutive, and it promotes the MTF-1 DNA sequence which acts as a transcription factor for the following promoter. Ideally, this sensor is coupled with an actuator or signal output device to detect lead in the cellular environment. false false _848_ 0 24160 9 Not in stock false We made sure that the following promoter bound to the transcription factor we chose. false Kelsey Pian component2428946 1 BBa_M36804 component2428947 1 BBa_M36412 component2428945 1 BBa_M36411 component2428948 1 BBa_M36413 annotation2428945 1 BBa_M36411 range2428945 1 1 40 annotation2428948 1 BBa_M36413 range2428948 1 2101 2143 annotation2428946 1 BBa_M36804 range2428946 1 49 61 annotation2428947 1 BBa_M36412 range2428947 1 68 2092 BBa_M36804 1 BBa_M36804 pRBS-SD1+6A 2014-04-29T11:00:00Z 2015-05-08T01:14:06Z This part comes from GeneDesigner and DNA 2.0 This is a prokaryotic ribosome binding site from GeneDesigner (made by DNA 2.0). It has a 6A tail to provide spacing. false false _848_ 0 22143 9 Not in stock false No considerations made. false Bradley Hammoor BBa_M36412 1 BBa_M36412 MTF1 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z MTF1 derives from the mouse genome. The sequence was back translated from amino acids to avoid introns. Codes for the transcription factor for the Pb (II) sensor. false false _848_ 0 24160 9 Not in stock false None false Kelsey Pian BBa_M36411 1 BBa_M36411 E. coli lac promoter 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z Gene Designer 2.0 The promoter is a standard promoter provided by the Gene Designer 2.0 software. It is constitutive. false false _848_ 0 24177 9 Not in stock false We needed a constitutive protein. false Rocco Cervantes BBa_M36413 1 BBa_M36413 MTF-1 regulated promoter 2014-10-22T11:00:00Z 2015-05-08T01:14:04Z We got this from the mouse genome. This promoter requires MTF-1 as the transcription factor. In conjunction with MTF-1, the promoter will being transcription. false false _848_ 0 24177 9 Not in stock false It needs to be coupled with MTF1 false Rocco Cervantes BBa_M36412_sequence 1 atgggtgaacacagccctgatgacaatattattttttttaaaggtgaagaggacgacctgacgccgcatgacaagatgctgcgctttgttgacgacaacggtctggttccgagcagcagcggtacggtgtacgaccgtaccacggtgctgatcgaacaggacccgggcaccttggaagatgatgaagatgatggccagtgcggcgagccgttgccgtttttggttgagggcgaggagggtttcctgatcgatcaagaggctatgtcgcaaggctatgtccaacatatcatctctccggaccagatccacttgacgattaatccgggtagcacgcctatgccgcgtaacattgagggtgcgaccctgaccctgcagtcggagtgcccggaaaccaagcgcaaggaagttaaacgttaccagtgcaccttcgagggttgtccgcgtacctacagcaccgcgggtaatctccgcacgcaccaaaagacccaccgcggcgagtatacctttgtctgcaaccaagagggttgcggtaaggcattcctgaccagctacagcttacgcattcacgtgcgcgtccatacgaaagagaaaccgttcgagtgcgacgtgcagggctgcgagaaagcgttcaataccctgtaccgtctgaaggcacaccaacgtctgcacaccggcaaaaccttcaactgtgagagccagggctgtagcaaatattttaccaccctgtctgatctgcgtaagcacatccgtacccacaccggcgagaagccgttccgttgcgaccatgacggttgtggcaaggcctttgctgcatctcaccatctgaaaacgcatgtgcgtactcacacgggtgagcgtccgttcttctgcccaagcaatggctgtgaaaagaccttcagcacgcagtattctctgaaatcccacatgaaaggtcatgacaataaaggcacggcgtacagcgcactgccgcaacacaacggctccgaagataccaaccactcgctgtatctgagcgaactgggtctgctgtccactgactccgagctgcaggaaaatagcagcagcacccaggaccaagatctgagcaccatcagcccggcaatcatttttgagtctatgtttcaaaacagcgatgatccgggcatccaagatgacccgctgcaaacggctgcgctgattgatagcttcaacggtgatgcagagagcgttattgacgtgcctccaccggcgggtaacagcgcgagccttagcctgccgctggtgctgcagagcggtatcagcgaaccgccgcagccgctgttgccggcgacggccccatctgcaccgccgccggccccgagcctgggtccgggtagccaaccggctgccttcggctccccaccggcactgctgcagccgccagaagtcccggttccgcatagcacgcagttcgcggcgaatcatcaagaatttctgccgcatccgcaggcaccgccgcaaacgattgtgccaggtctgagcgtcgttgcgggtgcgccagcaagcgcggcaacggtcgcttccgcagtcgctgcaccggcccctccgcagtccactaccgaaccgctgccggccatggttcagaccttgccgctgggtgcaaacagcgttctgactaacaacccgacgattaccattaccccgaccccgaacaccgcgattctgcagagctcgctggttatgggcgagcagaatttgcagtggattctgaatggtgcgacgtcaagcccgcagaaccaagaacagatccagcaagccagcaaggttgaacaagtctatttcgcgacggccgtgccggtggcgagcggtaccggcagcagcgtgcaacaaattggtctgtctgtgccggttatcattatcaaacaagaagaggcgtgccagtgtcaatgcgcgtgtcgtgatagcgcgaaagagcgcgctgccggtcgccgtaagggttgtagctccccgccaccgccggaaccgaatccgcagccgcctgacggtccgagcttgcagctgccgcct BBa_M36411_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtgga BBa_M36804_sequence 1 aggaggtaaaaaa BBa_M36413_sequence 1 cgatctctgcactccgccccgatagggacgtgaggcgggctgc BBa_M36414_sequence 1 ggctttacactttatgcttccggctcgtatgttgtgtggatactagagaggaggtaaaaaatactagatgggtgaacacagccctgatgacaatattattttttttaaaggtgaagaggacgacctgacgccgcatgacaagatgctgcgctttgttgacgacaacggtctggttccgagcagcagcggtacggtgtacgaccgtaccacggtgctgatcgaacaggacccgggcaccttggaagatgatgaagatgatggccagtgcggcgagccgttgccgtttttggttgagggcgaggagggtttcctgatcgatcaagaggctatgtcgcaaggctatgtccaacatatcatctctccggaccagatccacttgacgattaatccgggtagcacgcctatgccgcgtaacattgagggtgcgaccctgaccctgcagtcggagtgcccggaaaccaagcgcaaggaagttaaacgttaccagtgcaccttcgagggttgtccgcgtacctacagcaccgcgggtaatctccgcacgcaccaaaagacccaccgcggcgagtatacctttgtctgcaaccaagagggttgcggtaaggcattcctgaccagctacagcttacgcattcacgtgcgcgtccatacgaaagagaaaccgttcgagtgcgacgtgcagggctgcgagaaagcgttcaataccctgtaccgtctgaaggcacaccaacgtctgcacaccggcaaaaccttcaactgtgagagccagggctgtagcaaatattttaccaccctgtctgatctgcgtaagcacatccgtacccacaccggcgagaagccgttccgttgcgaccatgacggttgtggcaaggcctttgctgcatctcaccatctgaaaacgcatgtgcgtactcacacgggtgagcgtccgttcttctgcccaagcaatggctgtgaaaagaccttcagcacgcagtattctctgaaatcccacatgaaaggtcatgacaataaaggcacggcgtacagcgcactgccgcaacacaacggctccgaagataccaaccactcgctgtatctgagcgaactgggtctgctgtccactgactccgagctgcaggaaaatagcagcagcacccaggaccaagatctgagcaccatcagcccggcaatcatttttgagtctatgtttcaaaacagcgatgatccgggcatccaagatgacccgctgcaaacggctgcgctgattgatagcttcaacggtgatgcagagagcgttattgacgtgcctccaccggcgggtaacagcgcgagccttagcctgccgctggtgctgcagagcggtatcagcgaaccgccgcagccgctgttgccggcgacggccccatctgcaccgccgccggccccgagcctgggtccgggtagccaaccggctgccttcggctccccaccggcactgctgcagccgccagaagtcccggttccgcatagcacgcagttcgcggcgaatcatcaagaatttctgccgcatccgcaggcaccgccgcaaacgattgtgccaggtctgagcgtcgttgcgggtgcgccagcaagcgcggcaacggtcgcttccgcagtcgctgcaccggcccctccgcagtccactaccgaaccgctgccggccatggttcagaccttgccgctgggtgcaaacagcgttctgactaacaacccgacgattaccattaccccgaccccgaacaccgcgattctgcagagctcgctggttatgggcgagcagaatttgcagtggattctgaatggtgcgacgtcaagcccgcagaaccaagaacagatccagcaagccagcaaggttgaacaagtctatttcgcgacggccgtgccggtggcgagcggtaccggcagcagcgtgcaacaaattggtctgtctgtgccggttatcattatcaaacaagaagaggcgtgccagtgtcaatgcgcgtgtcgtgatagcgcgaaagagcgcgctgccggtcgccgtaagggttgtagctccccgccaccgccggaaccgaatccgcagccgcctgacggtccgagcttgcagctgccgccttactagagcgatctctgcactccgccccgatagggacgtgaggcgggctgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z