BBa_M36515
1
BBa_M36515
Silaffin 1 Peptide Encoding Gene
2012-12-06T12:00:00Z
2015-05-08T01:14:05Z
http://www.uniprot.org/uniprot/Q9SE35
This is the source of the amino acid sequence of silaffin 1 in marine diatoms. We reverse engineered this sequence to create our gene for E. coli
This gene codes for a peptide called silaffin 1. It is composed of silaffin 1A1, silaffin 1A2, and silaffin 1B peptides. This gene is naturally found in marine diatoms, which use silaffin in order to incorporate silica into various cellular structures. The silaffin 1 peptide precipitates silica when isolated from an organism and reacted with silicic acid.
false
false
_848_
0
15034
9
Not in stock
false
The silaffin 1 peptide amino acid sequence found in marine diatoms can be found online at http://www.uniprot.org/uniprot/Q9SE35. Our project entailed expressing silaffin 1 in E. coli, thus we derived the DNA sequence from the online amino acid sequence and codon optimized the sequence for E. coli. We also optimized to eliminate repeats and hidden stop codons.
false
Kevin Bui, Jordan Shapiro, Kevin Madrigal
BBa_M36515_sequence
1
aaacttaccgcaatcttcccactgctgtttaccgctgttggttattgcgcggcgcaaagtatcgccgacctggcggctgccaacctgtccaccgaagattcgaaatccgctcagcttatttcggcggactcttccgacgacgcttccgacagctctgtcgaatccgtcgacgccgcaagcagcgacgtatccggcagctcggtggaatccgtggacgtatcgggcagctccctggaaagcgtggacgtttctggctccagtctcgaatctgtggacgacagcagcgaggactccgaggaagaggagctgcgtatcctcagcagcaaaaaatcaggttcatactactcttacggtaccaagaagagcggctcgtattcgggctactctaccaaaaagtccgcgtcacgtcgtattttgtcaagcaagaagagcggctcttactccggttacagcaccaagaaaagcggttcccgtcgcatcctgtcttccaagaaatctgggtcctattctgggtccaagggctccaaacgacgtattctgtcatcaaaaaaatccggcagttacagcggctcaaaaggcagcaaacggcgcaacttgtccagcaagaagtctggttcttactctggttccaagggttcaaaacgtcgcattctttcctccaagaagtcaggttcgtactctggctccaaaggttccaaacgccgcaacctcagctcgaaaaaatctggctcctactctggcagcaaaggctccaagcgtcgtatcttgtctggtggcctgcgcggttccatgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z