BBa_M36517 1 BBa_M36517 Silaffin Actuator 2012-12-06T12:00:00Z 2015-05-08T01:14:05Z RBS: http://partsregistry.org/Part:BBa_M36516 silaffin encoding gene: http://partsregistry.org/Part:BBa_M36515 transcription terminator:http://partsregistry.org/Part:BBa_J61048 This device consists of the following parts in sequential order: a medium strength bicistronic RBS (BBa_M36516), a silaffin encoding gene (BBa_M36515), and a strong transcription terminator (BBa_J61048). false false _848_ 0 15034 9 Not in stock true We had to optimize for E. coli. This actuator takes in a PoPs signal and produces silaffin, which can then be extracted to precipitate silica. false Kevin Bui, Jordan Shapiro, Kevin Madrigal component2213676 1 BBa_M36516 component2213678 1 BBa_J61048 component2213677 1 BBa_M36515 annotation2213677 1 BBa_M36515 range2213677 1 97 891 annotation2213676 1 BBa_M36516 range2213676 1 1 88 annotation2213678 1 BBa_J61048 range2213678 1 900 1012 BBa_J61048 1 BBa_J61048 [rnpB-T1] Terminator 2007-02-20T12:00:00Z 2015-08-31T02:03:00Z bob bob false false _95_ 0 483 95 In stock false bob true John Anderson BBa_M36515 1 BBa_M36515 Silaffin 1 Peptide Encoding Gene 2012-12-06T12:00:00Z 2015-05-08T01:14:05Z http://www.uniprot.org/uniprot/Q9SE35 This is the source of the amino acid sequence of silaffin 1 in marine diatoms. We reverse engineered this sequence to create our gene for E. coli This gene codes for a peptide called silaffin 1. It is composed of silaffin 1A1, silaffin 1A2, and silaffin 1B peptides. This gene is naturally found in marine diatoms, which use silaffin in order to incorporate silica into various cellular structures. The silaffin 1 peptide precipitates silica when isolated from an organism and reacted with silicic acid. false false _848_ 0 15034 9 Not in stock false The silaffin 1 peptide amino acid sequence found in marine diatoms can be found online at http://www.uniprot.org/uniprot/Q9SE35. Our project entailed expressing silaffin 1 in E. coli, thus we derived the DNA sequence from the online amino acid sequence and codon optimized the sequence for E. coli. We also optimized to eliminate repeats and hidden stop codons. false Kevin Bui, Jordan Shapiro, Kevin Madrigal BBa_M36516 1 BBa_M36516 Bicistronic RBS #5 2012-12-06T12:00:00Z 2015-05-08T01:14:05Z http://io.biofab.org:5000/simple/bd_gois BCD5 BioFab characterized bicistronic ribosome binding site. Useful for its standardization and ability to be used in multiple organisms. It is medium strength with regards to the other bicistronic ribosome binding sites. false false _848_ 0 15034 9 Not in stock false Standardized RBS. false Kevin Bui BBa_M36515_sequence 1 aaacttaccgcaatcttcccactgctgtttaccgctgttggttattgcgcggcgcaaagtatcgccgacctggcggctgccaacctgtccaccgaagattcgaaatccgctcagcttatttcggcggactcttccgacgacgcttccgacagctctgtcgaatccgtcgacgccgcaagcagcgacgtatccggcagctcggtggaatccgtggacgtatcgggcagctccctggaaagcgtggacgtttctggctccagtctcgaatctgtggacgacagcagcgaggactccgaggaagaggagctgcgtatcctcagcagcaaaaaatcaggttcatactactcttacggtaccaagaagagcggctcgtattcgggctactctaccaaaaagtccgcgtcacgtcgtattttgtcaagcaagaagagcggctcttactccggttacagcaccaagaaaagcggttcccgtcgcatcctgtcttccaagaaatctgggtcctattctgggtccaagggctccaaacgacgtattctgtcatcaaaaaaatccggcagttacagcggctcaaaaggcagcaaacggcgcaacttgtccagcaagaagtctggttcttactctggttccaagggttcaaaacgtcgcattctttcctccaagaagtcaggttcgtactctggctccaaaggttccaaacgccgcaacctcagctcgaaaaaatctggctcctactctggcagcaaaggctccaagcgtcgtatcttgtctggtggcctgcgcggttccatgtaa BBa_J61048_sequence 1 ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_M36517_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaggggagggtttctaatgtactagagaaacttaccgcaatcttcccactgctgtttaccgctgttggttattgcgcggcgcaaagtatcgccgacctggcggctgccaacctgtccaccgaagattcgaaatccgctcagcttatttcggcggactcttccgacgacgcttccgacagctctgtcgaatccgtcgacgccgcaagcagcgacgtatccggcagctcggtggaatccgtggacgtatcgggcagctccctggaaagcgtggacgtttctggctccagtctcgaatctgtggacgacagcagcgaggactccgaggaagaggagctgcgtatcctcagcagcaaaaaatcaggttcatactactcttacggtaccaagaagagcggctcgtattcgggctactctaccaaaaagtccgcgtcacgtcgtattttgtcaagcaagaagagcggctcttactccggttacagcaccaagaaaagcggttcccgtcgcatcctgtcttccaagaaatctgggtcctattctgggtccaagggctccaaacgacgtattctgtcatcaaaaaaatccggcagttacagcggctcaaaaggcagcaaacggcgcaacttgtccagcaagaagtctggttcttactctggttccaagggttcaaaacgtcgcattctttcctccaagaagtcaggttcgtactctggctccaaaggttccaaacgccgcaacctcagctcgaaaaaatctggctcctactctggcagcaaaggctccaagcgtcgtatcttgtctggtggcctgcgcggttccatgtaatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa BBa_M36516_sequence 1 gggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcaggggagggtttctaatg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z