BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986785
1
-35
range1986785
1
20
25
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
BBa_M36528
1
BBa_M36528
In the presence of ATC, produces lacI
2015-10-21T11:00:00Z
2015-12-03T07:25:47Z
LacI Gene: Part:BBa_K642001:Design (parts.igem.org)
When this part is put into a yeast cell that has TetR, the TetR will bind to the operator and prevent gene expression. By adding ATC, gene expression will be allowed to proceed and LacI protein will be made.
true
false
_848_
29107
29107
9
false
Ensured codons were optimized for yeast. Short random (10 bp) region RBS and the actual gene put in. Eliminated restriction enzyme sites (Bbs1, Bsa1,BsmB1).
false
Valerie Wesley Scott
component2478335
1
BBa_R0040
component2478341
1
BBa_M36525
component2478340
1
BBa_M36526
annotation2478335
1
BBa_R0040
range2478335
1
1
54
annotation2478340
1
BBa_M36526
range2478340
1
63
74
annotation2478341
1
BBa_M36525
range2478341
1
81
1163
BBa_M36525
1
BBa_M36525
LacI
2015-10-22T11:00:00Z
2015-12-03T07:17:53Z
A selected portion of the BBa_K642001 part on iGem's Registry of Standard Biological Parts.
The coding DNA sequence for the LacI protein. This protein can be used to bind to a pLac operator and repress expression of another gene.
false
false
_848_
29107
29107
9
false
Had to ensure it was yeast optimized.
false
Valerie Wesley Scott
BBa_M36526
1
BBa_M36526
Kozak
2015-10-22T11:00:00Z
2015-12-03T07:22:38Z
Was from part BBa_K792012 on igem's Registry of Standard Biological Parts
A kozak sequence for yeast. Is missing the last three base pairs (ATG) which should be the first three of your protein coding section. Should be placed directly before this protein coding section
true
false
_848_
29107
29107
9
false
Wanted to ensure that it would work in yeast and several other igem projects said it was used successfully.
false
Valerie Wesley Scott
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_M36525_sequence
1
atgaaaccagtcacgttatatgacgttgctgaatatgccggtgtttcttaccaaaccgtgtctcgtgtagtgaatcaagccagtcatgtcagtgccaagacacgtgagaaggtcgaagctgcaatggctgagttaaattacatccccaacagggttgctcaacaactggctggcaaacagagtctattgataggagtggcaacttcttccttagctttgcatgcaccatcccagatagtggctgcaatcaagtctagggctgatcaactaggtgctagtgtagttgtctctatggtcgagagatccggcgttgaagcttgtaaggctgctgttcataatctattagcacaaagagtcagtggtctgattataaactacccccttgatgatcaagacgcaattgctgtcgaagcagcatgtacaaacgttcccgccttgtttttagatgttagtgaccagactccaattaactccattattttttctcacgaagatggtactagattaggcgtcgaacacctggtagcccttggacatcaacaaatagccttattagcaggtcctttgagttctgtatctgcaagactaagattggcaggctggcacaaatacttgacgagaaaccaaatacaacccattgctgaaagagaaggtgattggagtgcaatgagtggctttcaacaaaccatgcaaatgttgaatgagggtatagttccgaccgctatgcttgttgctaatgaccaaatggctcttggtgcgatgagagccataacagaaagtggattgagagttggcgccgatatttcagttgttggttatgatgatactgaagattctagctgttatatccctccattgaccacgatcaagcaggactttagattgttaggtcaaacaagtgtggatagattgctacaactttctcaggggcaggctgttaagggtaaccaattgttgcctgtttctttggtcaaacgtaagaccaccttagctccaaatactcaaaccgcttctcctagagcattggcggattccttaatgcagttggccagacaagtttccagattagaatcaggccaaacctaa
BBa_M36528_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagcccgccgccacctactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagact
BBa_M36526_sequence
1
cccgccgccacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z