BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986785 1 -35 range1986785 1 20 25 annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986783 1 TetR 1 range1986783 1 1 19 BBa_M36528 1 BBa_M36528 In the presence of ATC, produces lacI 2015-10-21T11:00:00Z 2015-12-03T07:25:47Z LacI Gene: Part:BBa_K642001:Design (parts.igem.org) When this part is put into a yeast cell that has TetR, the TetR will bind to the operator and prevent gene expression. By adding ATC, gene expression will be allowed to proceed and LacI protein will be made. true false _848_ 29107 29107 9 false Ensured codons were optimized for yeast. Short random (10 bp) region RBS and the actual gene put in. Eliminated restriction enzyme sites (Bbs1, Bsa1,BsmB1). false Valerie Wesley Scott component2478335 1 BBa_R0040 component2478341 1 BBa_M36525 component2478340 1 BBa_M36526 annotation2478335 1 BBa_R0040 range2478335 1 1 54 annotation2478340 1 BBa_M36526 range2478340 1 63 74 annotation2478341 1 BBa_M36525 range2478341 1 81 1163 BBa_M36525 1 BBa_M36525 LacI 2015-10-22T11:00:00Z 2015-12-03T07:17:53Z A selected portion of the BBa_K642001 part on iGem's Registry of Standard Biological Parts. The coding DNA sequence for the LacI protein. This protein can be used to bind to a pLac operator and repress expression of another gene. false false _848_ 29107 29107 9 false Had to ensure it was yeast optimized. false Valerie Wesley Scott BBa_M36526 1 BBa_M36526 Kozak 2015-10-22T11:00:00Z 2015-12-03T07:22:38Z Was from part BBa_K792012 on igem's Registry of Standard Biological Parts A kozak sequence for yeast. Is missing the last three base pairs (ATG) which should be the first three of your protein coding section. Should be placed directly before this protein coding section true false _848_ 29107 29107 9 false Wanted to ensure that it would work in yeast and several other igem projects said it was used successfully. false Valerie Wesley Scott BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_M36525_sequence 1 atgaaaccagtcacgttatatgacgttgctgaatatgccggtgtttcttaccaaaccgtgtctcgtgtagtgaatcaagccagtcatgtcagtgccaagacacgtgagaaggtcgaagctgcaatggctgagttaaattacatccccaacagggttgctcaacaactggctggcaaacagagtctattgataggagtggcaacttcttccttagctttgcatgcaccatcccagatagtggctgcaatcaagtctagggctgatcaactaggtgctagtgtagttgtctctatggtcgagagatccggcgttgaagcttgtaaggctgctgttcataatctattagcacaaagagtcagtggtctgattataaactacccccttgatgatcaagacgcaattgctgtcgaagcagcatgtacaaacgttcccgccttgtttttagatgttagtgaccagactccaattaactccattattttttctcacgaagatggtactagattaggcgtcgaacacctggtagcccttggacatcaacaaatagccttattagcaggtcctttgagttctgtatctgcaagactaagattggcaggctggcacaaatacttgacgagaaaccaaatacaacccattgctgaaagagaaggtgattggagtgcaatgagtggctttcaacaaaccatgcaaatgttgaatgagggtatagttccgaccgctatgcttgttgctaatgaccaaatggctcttggtgcgatgagagccataacagaaagtggattgagagttggcgccgatatttcagttgttggttatgatgatactgaagattctagctgttatatccctccattgaccacgatcaagcaggactttagattgttaggtcaaacaagtgtggatagattgctacaactttctcaggggcaggctgttaagggtaaccaattgttgcctgtttctttggtcaaacgtaagaccaccttagctccaaatactcaaaccgcttctcctagagcattggcggattccttaatgcagttggccagacaagtttccagattagaatcaggccaaacctaa BBa_M36528_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagcccgccgccacctactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagact BBa_M36526_sequence 1 cccgccgccacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z